• 한국균학회지
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• 제44권3호
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• pp.166-170
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• 2016

Ectomycorrhizal fungi are associated with plants roots and acquire significant amounts of nitrogen sources from the soil. For artificial cultivation, mass production of ectomycorrhizal fungi in liquid media is required. We studied the edible ectomycorrhizal mushrooms Hygrophorus russula, Ramaria fumigata, Sarcodon aspratus, and Tricholoma matsutake. All strains except S. aspratus NIFoS 2031 grew generally well on modified Melin-Norkran's (MMN) medium compared to on other media. All strains analyzed in this study showed significantly higher growth on organic nitrogen. Specifically, two strains of H. russula significantly responded to both tryptone and neopeptone media. Among different species and strains, there were clear differences in the capacity to grow on animal-based organic nitrogen sources.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.103.2-103.2
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• 2007

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• 식품과학과 산업
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• 제51권4호
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• pp.334-342
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• 2018

People in Korea, a peninsular, have acquired a variety of marine food resources including seaweeds. Ecklonia stolonifera, a brown algae, is commonly dwelling in Korean coasts and their cultivation methods were developed for a mass-production. Recently, studies have revealed that Ecklonia stolonifera is a promising material for the development of health functional foods. In an effort to carefully review the current understating in the effects and mechanisms of Ecklonia stolonifera on liver functions by deduction from relevant literatures, the effective components were identified as phlorotannins, including dieckol, eckstolonol, eckol, phlorofucofuroeckol A, and phlorosterol. Their aiding action on the hepatic functions is categorized as follows. A) Regulation of oxidative stress by anti-oxidant capacity, B) Protection of hepatocytes from toxins, C) Prevention of alcoholic fatty liver and fibrogenesis, D) Regulation of chronic disease by improvement of inflammatory responses and lipid metabolisms, and E) indirect benefit conferred by a personal total wellness.

• ALGAE
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• 제36권4호
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• pp.327-332
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• 2021

Raw material of gelidioid red algae yielding high-quality agar has been in short supply due to overharvesting, but in situ farming of gelidioids has not been practical due to their slow growth. To produce vegetative seedstock of a cosmopolitan species, Pterocladiella capillacea, we investigated the number and length of regenerated branches arising from sectioned fragments during 3 weeks of laboratory culture at 10, 15, 20, and 25℃. All sectioned fragments formed axis-like branches mostly from the upper cut edge and stolon-like branches mostly from the lower cut edge, showing a high capacity of regeneration and intrinsic bipolarity. At 20℃, the number of regenerated branches increased to 2.74 ± 1.29 on the upper cut edge and 4.26 ± 2.66 on the lower cut edge. Our study reveals that the use of fragments bearing regenerated branches as seedstock can be a simple method to initiate fast propagation for mass cultivation in the sea or outdoor tank.

• 한국염색가공학회지
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• 제34권2호
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• pp.77-92
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• 2022

Natural colorants are regarded as substitutes for synthetic colorants in the fields of fiber dyeing, hair dyeing, cosmetic, and food industries. The use of natural colorants has been increased in view of global environment and sustainability. However, scientific approach to the production is required because the supply of natural colorants depends on the climate, soil, and cultivation methods. This brief review contains the advances in science and technology related to natural colorants including characterization, analysis, CIEL^*a^*b^* and Munsell color systems, and future prospects. For the characterization and the analysis of natural colorants, chromatography, some spectroscopic techniques (UV-Vis, FT-IR, NMR), mass spectrometry, and X-ray diffraction are generally used.

• KSBB Journal
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• 제21권6호
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• pp.479-483
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• 2006

본 연구는 효모의 세포벽에 존재하는 본 연구는 효모의 세포벽에 존재하는 ${\beta}-Glucan$을 대량생산하기 위해 Saccharomyces cerevisiae JUL3의 균체량을 증가시키기 위한 연구를 수행하였다. S. cerevisiae JUL3의 배양학적 특성을 알아보기 위해 kinetic parameter를 조사하였을 때 specific growth rate (${\mu}$)는 $0.145\;h^{-1}$, yield ($Y_{x/s}$)는 0.332 g/g, glucose 소모속도($q_{s}$)는 $0.437\;h^{-1}$이며 productivity (P)는 $0.4827\;g/{\ell}{\cdot}h$을 나타내었다. 균체량이 가장 높게 나타난 탄소원과 질소원은 고과당 (high fructose syrup)과 yeast extract이었다. 효모균체 대량생산 및 scale up을 위하여 반응표면 분석법을 통하여 고과당과 yeast extract에 대한 최적농도를 조사하였다. 균체량이 가장 많이 생산되어질 수 있는 조건으로 제시된 고과당과 yeast extract의 최적 농도는 각각 8.0 %와 5.2 %였으며, 이 때 예측되는 균체량은 $16.95\;g/{\ell}$ 이었다.

• Mycobiology
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• 제38권1호
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• pp.7-12
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• 2010

A green mold species that has not previously been reported in Korea was isolated from oak log beds used for shiitake (Lentinula edodes) cultivation that were infested by mushroom flies. In this study, we identify the mold species as Gliocladium viride (an anamorph of Hypocrea lutea) and describe its mycological properties. The fungus was cottony on both potato dextrose agar (PDA) and Czapek yeast extract agar (CYA), but was colored white on PDA and became yellowish green and brown on CYA. Mycelial growth on PDA attained a diameter of 73 mm at $30^{\circ}C$ after 5 days. The fungus grew faster on malt extract agar (> 80 mm, 5 days at $25^{\circ}C$) compared to CYA and PDA (< 68 mm, 5 days at $25^{\circ}C$). Penicillate conidiophores of the fungus are hyaline, smooth walled, branching above typically in four stages, and $120\sim240\;{\mu}m$ in length. Club-shaped or slender phialides are formed on the metulae. Conidia of the fungus were ovate and elliptic, yellowish brown and green, and $2.5\sim3.0\;{\mu}m\times1.8\sim2.3\;{\mu}m$ in size. Typically, slimy conidia are formed in a mass and colored brown to dark green to almost black. The internal transcribed spacer rDNA and translation elongation factor 1 alpha gene sequences of the fungus isolated here show 99% identity with previously identified G. viride strains.

• 한국자원식물학회지
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• 제16권1호
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• pp.1-7
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• 2003

본 연구는 굼벵이를 이용하여 동충하초의 자실체를 유도하기 위하여 3종의 동충하초(Cordyceps militaris) CHO-7208, CHO-7845, CHO-7846를 사용하여 배양조건을 조사하였다. PDA배지에서 13일간 배양 결과 CHO-7208는 25 $\pm$2mm, CHO-7845는 26$\pm$2mm와 CHO-7846은 16$\pm$2mm 생장하였으며 굼벵이를 배지로서 이용하기 위해서는 전분이나 밀가루, 쌀을 이용하여 사육을 했을 때 전분의 생존율이 높았다. 굼벵이 배지에서는 CHO-7298과 CHO-7846에서 자실체가 발생하였으며 27일간 생장량은 각각 51$\pm$5mm와 51 $\pm$5mm로 생장하였다. 또한 굼벵이 에서의 자실체 발생은 기중균사 형성이 왕성한 것이 균주로서 적합하였으며 굼벵이 배지에 동충하초를 배양하여도 자실체의 발생은 양호하였다.

• Journal of Plant Biotechnology
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• 제42권2호
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• pp.111-116
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• 2015

Interest and great demand for blueberry (Vaccinium corymbosum) have increased, as V. corymbosum is now one of the most economically important crops in Korea. It is expected that blueberry production and the area planted for cultivation will increase consistently in the years ahead because of high profitability and the consumer's demand for healthy ingredients. Effective mass production of blueberry is urgently needed for commercial cultivation establishment, but a main limitation is lack of a propagation system that produces a disease-free plant material for commercial plantation. A large amount of research has focused entirely on developing tissue culture techniques for blueberry propagation. However, controlling fungal and bacterial contamination of woody plant material is extremely difficult. Our study was conducted to investigate the effect of biocide addition during the in vitro culture of blueberry on plantlet growth and contamination occurrence. Four biocides, including Plant Preservative Mixture ($PPM^{TM}$), vancomycin, nystatin and penicillin G, were used in varying concentrations during the in vitro propagation of blueberry. When nystatin was added into the medium at low concentrations, the overall growth of blueberry plantlets was retarded. Addition of vancomycin and penicillin G in high concentrations decreased contamination but induced plantlet mortality. On the other hand, when 1ml/L $PPM^{TM}$ was added, the growth characteristics of blueberry plantlets did not significantly differ from non-treatment (control), and the contamination occurrence rate was very low. From these results, we found that the addition of the appropriate biocide could provide an effective method to reduce contamination in the culture process, thereby raising in vitro production efficiency.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1997년도 하계 학술대회 논문집
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• pp.89-98
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• 1997

This study was conducted to develop an automatic nutrient-solution control system for small-scale growers. The nutrient-solution control system consisted of a low-cost and precise metering device and a personal computer. The system controlled electric conductivity(EC) and pH of nutrient-solution based on the time-based feedback control method with the information about temperature, EC, and pH of the nutrient-soIution. The performance of the nutrient-solution control system was evaluated through the control of EC and pH while compared with those of commercial nutrient-solution control system. Also an experimental cultivation of tomato was conducted to verify and to improve the developed system. Results of this study were as follows. 1. An automatic nutrient-solution control system based on a low-cost and precise metering device was developed. 2. The developed system controlled EC and pH within $\pm$0.05 mS/cm and $\pm$0.2 pH full scale error respectively at $24^{/circ}C$. 3. When using the commercial system, the controlled values of EC and pH of the 500l of water were 1.29 mS/cm and 6.1 pH for the setting points of 1.4 mS/cm and 6.0 pH respectively at $22^{/circ}C$. 4. The developed nutrient-solution control system showed $\pm$0.05 mS/cm of deviation from the setting EC value over the experimental cultivation period. 5. The deviation from the average values of Ca and Mg mass content in the several nutrient-solution were 0.5% and 1.8% respectively.