• Biomedical Science Letters
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• v.8 no.3
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• pp.179-184
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• 2002

We investigated the morphological changes and TUNEL reaction of apoptotic cells in the liver of D-galactosamine (20 mg/mouse) and lipopolysaccharide (5 $\mu\textrm{g}$/mouse)-treated 30 mice (BALB/c), and in additioa also of apoptotic cells in kidney and spleen. The livers and other some organs of mice at 6, 12, 24, 48 and 72 hrs after treatment were collected and were fixed with 10% neutral formalin and paraffin sections were stained with hematoxylin-eosin or terminal deoxynucleotidly transferase-mediated dUTP nick end labeling (TUNEL) method. Morphological changes in apoptotic hepatocytes were chondensation of nuclei and density of cytoplasms, then the margination and pyknosis of chromatin, the formation of half-moon- or horse-shoe- or ship-like shapes of condensed chromatin mass, lastly formation of apoptotic bodies, disappearance of nuclear envelopes, decrease of stainability, then lysis and disappearance of apoptotic bodies. TUNEL positive reactions of hepatocytes were appeared first moderate in uncondensed hepatocytes, severe in condensed hepatocytes, moderate in chromatin-marginated hepatocytes. These reactions also were appeared moderate in hepatocytes with half-moon- or horse-shoe- or ship-like pyknotic chromatin mass or apoptotic bodies, and mild or negative in hepatocytes with lysed apoptotic bodies or with disappeared nuclear envelopes. Consequently these results suggested that TUNEL positive reactions of hepatocytes appeared at more early stages than appearance of chromatin condensation and disappeared at more early stage than disappearance of histological findings of apoptosis. We also confirmed that the differentiation of apoptotic cells from normal healthy cells of Kupffer cells and vascular endothelial cells in liver, reticular cells and lymphocytes in spleen and epithelial cells of tubules and ducts in kidney was impossible in H-E preparations but was possible in TUNEL preparations.

• Korean Journal of Food Science and Technology
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• v.25 no.1
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• pp.52-56
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• 1993

Flavor compounds formed in the ramyon fried in palm oil at $148-150^{\circ}C$ for 1 minute during storage at $65^{\circ}C$ were isolated and identified by gas chromatography-mass spectrometry. Their changes in the amount when the ramyon was stored at 20, 40 and $65^{\circ}C$ were also studied by using static headspace gas chromatography. Pentane, hexane, butanal, heptane, 1-pentanol, hexanal, and octane were formed during $65^{\circ}C$ storage of the ramyon and they were thought to be from linoleic and oleic acid present in ramyon. Formation of the flavor compounds was shown to increase with the storage temperature and/or storage time. Hexanal showed the highest correlation with the sensory score(r=0.87).

• Advances in concrete construction
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• v.10 no.5
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• pp.443-454
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• 2020

In recent years, many studies have been done on the performance of concrete containing glass powder (GP). For the purpose of widespread use of GP in concrete mixes, a knowledge of the performance of such a mixture after a fire is essential for the perspective of structural use. This research work was carried out to evaluate the performance of High Performance Concrete (HPC) made with GP after being exposed to elevated temperature. The studied mixtures include partial replacement of cement by GP with up to 30%. The mechanical performance and structural alterations were assessed after high temperature treatment from 200℃ to 800℃. The mechanical performance was evaluated by testing the specimens to the compressive and tensile strength. In addition, the mass loss and the porosity were measured to notice the structural alterations. Changes in microstructure due to temperature was also investigated by the X-ray diffraction (XRD) and thermal gravimetric analyses (TGA) as well as porosity adsorption tests. The results of the concrete strength tests showed a slight difference in compressive strength and the same tensile strength performance when replacing a part of the cement by GP. However, after high temperature exposition, concrete with GP showed better performance than the reference concrete for temperature below 600℃. But, after heating at 800℃, the strength of the concrete with GP drop slightly more than reference concrete. This is accompanied by an important increase in mass loss and water porosity. After the microstructure analysis, no important changes happened differently for concrete with GP at high temperature except a new calcium silica form appears after the 800℃ heating.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.2
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• pp.272-278
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• 2011

Bovine whey protein expression patterns of colostrum are much different from that of milk. Moreover, bovine colostrum is an important source of protective, nutritional and developmental factors for the newborn. However, to our knowledge, no research has been performed to date using a comparative proteomic method on the changes in the bovine whey proteome during the transition from colostrum to milk. This study therefore separated whey protein of days 1, 3, 7 and 21 after calving using two dimension electrophoresis. Differentially expressed proteins at different collection times were identified using high-performance liquid chromatography in tandem with mass spectrometry (LC/MS) and validated by enzyme-linked immunosorbent assay (ELISA) in order to understand the developmental changes in the bovine whey proteome during the transition from colostrum to milk. The expression patterns of whey protein of days 1 and 3 post-partum were similar except that immunoglobulin G was down-regulated on day 3, and four proteins were found to be down-regulated on days 7 and 21 compared with day 1 after delivering, including immunoglobulin G, immunoglobulin M, albumin, and lactotransferrin, which are involved in immunity and molecule transport. The results of this study confirm the comparative proteomic method has the advantage over other methods such as ELISA and immunoassays in that it can simultaneously detect more differentially expressed proteins. In addition, the difference in composition of milk indicates a need for adjustment of the colostrum feeding regimen to ensure a protective immunological status for newborn calves.

• Journal of Digital Convergence
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• v.18 no.2
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• pp.427-437
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• 2020

This article researched the changes of terrestrial local broadcasting companies in a crisis of management. According to the following: First, the structure of the internal organization of local broadcasting companies has been changed to put more emphasis on 'management'. Second, most of the interviewees responded that this change was an inevitable choice. However, some responded that their 'role identity' was changing in a negative direction. Third, these changes have led to the emergence of programs that could undermine public interest. It also showed an increase in the production of 'funding-based' programs and 'mobile first' programs.

• Journal of The Korean Society of Integrative Medicine
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• v.8 no.3
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• pp.83-91
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• 2020

Purpose : To identify changes and relationships in the fat mass and obesity associated (FTO) gene polymorphism, body composition, and physical fitness from childhood to adolescence over a three-year period spanning elementary school to middle school (2015-2018) Methods : A total of 84 male student participants were divided into two groups based on FTO genotype: aa+at (group A) and tt (group T) and tracked down. Body composition, cardiovascular endurance, flexibility, muscle strength, power, and other characteristics were measured in the two groups in both 2015 and 2018, respectively, and the changes over the three-year period were analyzed and compared. Results : Increases in height and weight did not differ significantly between the two groups, but body mass index (BMI) was significantly higher in group A (p=.035). With regard to physical fitness, there was no significant difference in flexibility, but cardiovascular endurance, strength, and power were significantly higher in group T (p<.001, p=.063, and p=.040, respectively). Conclusion : Group A is more likely to become obese than group T because of their lower level of physical fitness and increased BMI relative to group T. This result supports previous studies showing that group A has a relatively low level of physical activity and a greater tendency to eat fatty foods as compared with group T. Therefore, we suggest that the FTO gene polymorphism should be identified early and that students educated on diet and physical activity to help prevent adult obesity.

• Journal of Environmental Science International
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• v.32 no.4
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• pp.205-220
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• 2023

In this study, impact of the COVID-19 outbreak on PM_2.5 mass and its five chemical components (NH₄⁺, NO₃^-, SO₄^2-, OC, EC) in Busan was evaluated, and compared with that of Seoul. The study period over the recent three years was sub-divided into two periods: Pre-COVID (2018~2019) and COVID (2020) periods, and the differences in observed annual and monthly variations between the two periods were explored here. The results indicated that annual mean PM_2.5 mass concentrations decreased during the COVID period by 16% in Seoul and 29% in Busan, and the satellite-observed annual average of aerosol optical depth (AOD) over the Korean Peninsula also decreased by approximately more than 10% compared with that of the Pre-COVID period. All of the five chemical components decreased but no particular changes were found in their fractions occupied during the COVID period. However, over the Lock-down period (2020-March), the sulfate fraction decreased in Seoul, mostly reflecting the recent Chinese trends of aerosol characteristics, whereas the nitrate fraction considerably decreased in Busan, which was attributable to the local emission changes and their variabilities in Busan. Other meteorological characteristics such as higher frequencies of easterly winds in the Busan area during the COVID period were also discussed in comparison with those in the Seoul area.

• Korean Journal of Food Science and Technology
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• v.22 no.4
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• pp.445-450
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• 1990

Changes in weight, volume and density of petiole tissue of Chinese cabbage during salting and blanching were investigated. Rapid changes in mass and volume occurred within 4 hours during salting in 5% salt solution and the changes were nearly completed after 8h. After salting, the reduction of mass and volume ranged between $22{\sim}27%\;and\;22{\sim}35%$, respectively. Average density of the sample was found to be 0.88g/ml, and increased to 1.020g/ml after salting. Air content of the sample ranged from 0.093 to 0.120ml/ml cabbage, and about 70% of the initial content was expelled from the tissue by salting. The changes of physical properties of the cabbage during steam blanching were similar to those during salting, but their relative values were smaller. A linearization model for physical changes during salting and blanching was proposed.

• Journal of Life Science
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• v.20 no.6
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• pp.885-893
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• 2010

This study measured physical characteristics and physical composition of 16 middle-aged obese women using a body composition analyzer. TC, TG, HDL-C and LDL-C were investigated in order to analyze components of serum liquids. The study also compared changes regarding cardiovascular risk factors before and after a 12-week exercise program by measuring insulin and intensity of TNF-$\alpha$. To examine such changes, the study carried out a 12-week, complex training program for middle-aged obese women who did not regularly exercise. The results of the study were as follows: 1) after 12 weeks of complex training, in changes of physical characteristics, weight, BMI and WHR were significantly reduced. Systolic blood pressure and diastolic blood pressure increased, but there were no significant differences; 2) after 12 weeks of complex training, in changes of physical composition, body fat mass and body fat rate were significantly reduced and fat free mass was significantly increased; 3) after 12 weeks of complex training, in changes of serum liquids, TC, TG and LDL-C were significantly reduced. HDL-C increased, but this was not statistically significant; 4) after 12 weeks of complex training, in changes of cardiovascular risk factors, insulin and TNF-$\alpha$ were significantly reduced.

• Clinical and Experimental Pediatrics
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• v.57 no.5
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• pp.232-239
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• 2014

Purpose: Mitochondrial disease (MD) and Duchenne muscular dystrophy (DMD) are often associated with cardiomyopathy, but the myocardial variability has not been isolated to a specific characteristic. We evaluated the left ventricular (LV) mass by echocardiography to identify the general distribution and functional changes of the myocardium in patients with MD or DMD. Methods: We retrospectively evaluated the echocardiographic data of 90 children with MD and 42 with DMD. Using two-dimensional echocardiography, including time-motion (M) mode and Doppler measurements, we estimated the LV mass, ratio of early to late mitral filling velocities (E/A), ratio of early mitral filling velocity to early diastolic mitral annular velocity (E/Ea), stroke volume, and cardiac output. A "z score" was generated using the lambda-mu-sigma method to standardize the LV mass with respect to body size. Results: The LV mass-for-height z scores were significantly below normal in children with MD ($-1.02{\pm}1.52$, P<0.001) or DMD ($-0.82{\pm}1.61$, P =0.002), as were the LV mass-for-lean body-mass z scores. The body mass index (BMI)-for-age z scores were far below normal and were directly proportional to the LV mass-for-height z scores in both patients with MD (R =0.377, P<0.001) and those with DMD (R =0.330, P=0.033). The LV mass-for-height z score correlated positively with the stroke volume index (R =0.462, P<0.001) and cardiac index (R =0.358, P<0.001). Conclusion: LV myocardial atrophy is present in patients with MD and those with DMD and may be closely associated with low BMI. The insufficient LV mass for body size might indicate deterioration of systolic function in these patients.