• Mass Spectrometry Letters
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• v.3 no.4
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• pp.93-100
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• 2012

Cellular processes such as proliferation, differentiation, and adaptation to environmental changes are regulated by protein phosphorylation. In order to enhance the understanding of molecular dynamics for biological process in detail, it is necessary to develop sensitive and comprehensive analytical methods for the determination of protein phosphorylation. Neural stem cells hold great promise for neural repair following an injury or disease. In this study, we made differentiated oligodendrocytes from human neural stem cells using over-expression of olig2 gene. We confirmed using quantitative phosphoproteome analysis approach that combines stable isotope labeling by amino acids in cell culture (SILAC) and $TiO_2$ micro-column for phosphopeptide enrichment with $MS^2$ and $MS^3$ mass spectrometry. We detected 275 phosphopeptides which were modulated at least 2-fold between human neural stem cells and oligodendrocytes. Among them, 23 phosphoproteins were up-regulated in oligodendrocytes and 79 phosphoproteins were up-regulated in F3 cells.

• Journal of Acupuncture Research
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• v.26 no.2
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• pp.171-179
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• 2009

Objectives: The purpose of this study was to investigate the changes of obesity, blood composition and physical ability in obese children on body weight control program. Methods : The body weight control program included exercise, nutrition education and auricular acupuncture was performed 4 times a week during 12 weeks. 32 obese children whose body fat ratio were over 30% participated in this study. Measuring materials were body composition(weight, muscle mass, fat mass, fat ratio, BMI), physical measurement(height, waist circumference, chest circumference, hip circumference), physical ability(grip strength, sit-ups, sitting trunk flexion, sit-down stand-up test, standing long jump, opened eyes foot balance, side step), blood composition(WBC, Hb, ESR, Total Cholesterol, TG, GOT, GPT, BUN, Creatinine, Glucose) and survey(changes of eating habits, changes of living habits, knowledge of obesity, self-confidence, self-esteem). Results : From the results, positive changes were made in BMI, height, waist circumferences, chest circumferences, hip circumferences, sit-ups, sitting trunk flexion, sit-down stand-up test, standing long jump, opened eyes foot balance, side step, Hb, ESR, GOT, GPT and changes of eating habits through the body weight control program. Conclusions : This results suggest that body weight control program including exercise, nutrition education and auricular acupuncture may be effective for helping obese children.

• Journal of Oriental Neuropsychiatry
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• v.3 no.1
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• pp.25-45
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• 1992

Many phychiatrists have reported that the change of serotonin concentration would cause mental disorder and affect the pathological conditions such as schizophrenia, depression and eating behabior. The end product of serotonin metabolism was excreted as 5-HIAA in urine. Serum lipids, according to the report, were concerned with obesity, said it was. This study aims to observe the changes of plasma serotonin, urinary 5-HIAA and serum lipids, making use of Fat Cell Mass rate of 27 normal persons and 42 psychosomatic patients. For this, I also observed the change of serotonin, 5-HIAA and lipids of the psychosomatic patients by the use of 3 kinds of herbs as treatment medication on the basis of physical symptoms and the results were obtained as follows; 1. Urinary 5-HIAA is correlated with the body water rate(r=0.381), while reversely correlated with the Fat Cell Mass rate(r=-0.330). 2. Compared the control group with the patients group for the serum lipids value, they showed the significant results ; $146.4{\pm}5.71$ mg/dl and $166.9{\pm}6.56$ mg/dl in the total cholestrol value over- weights, $471.2{\pm}42.99$ mg/dl in the total lipid value, and $158.1{\pm}6.50$ mg/dl and $181.1{\pm}6.30$ mg/dl in the phospholipid of the obesity, respectively. 3. With comparison of each group to other group to the others for Fat Cell Mass rate, the total cholesterol showed the significant differences when the Fat Cell Mass rate was 20% or more, HDL-cholesterol value when 30% or more, and triglyceride when 30% or more, respectively. 4. there was significant variations in the relations between Fat Cell Mass rate and body water, which body mass index was increased as the body water was decreased. 5. Fat Cell Mass rate was correlated with Cholesterol(r = 0.420), triglyceride (r = 0.443), and $\beta$-lipoprotein(r = 0.450) of serum lipids, while reversely correlated with HDL -Cholesterol(r = -0.354) and urinary 5- HIAA had the correlation coefficient of -0.330. 6. What related with body water rate urinary 5-HIAA (r = 0.381) and $\beta$-lipoprotein(r = -0.405). 7. there were significant changes in the total cholesterol value and HDL-Cholesterol Value of serum lipids after dose of Ondamtang, significant increase in the 5-HIAA value after dose of Samulansintang, and significant devrease in the Trigylceride value of serum lipids after dose of Shihosogansan. As a result, it was seen that there was direct correlations among the Fat Cell Mass, urinary 5-HIAA, and serum lipids and stress from the mental conditions was not correlated directly to Serotonin, 5-HIAA, and serum lipids. I would like to conclude, therefore, that the detailed study should be performed on the function of serotonin of hypothalamus.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.73-79
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• 2012

Seasonal changes in biochemical composition of muscle, gonad-viceral, mass and whole body of the cultured ark shell, Scapharca broughtonii in the Gamag bay of Yeosu city were studied from December 2008 to November 2009 in relation to environmental condition and reproductive cycles. Average monthly water temperature in the winter was in the range of $7-12^{\circ}C$ and $20-25^{\circ}C$ in the summer, while the salinity fluctuated in the range of 30.1%-33.8‰ on the average. Seasonal fluctuation of the concentration of nutrient salt was the highest in September ($13.04{\mu}g/L$) with average annual concentration of $4.6{\mu}g/L$. The main spawning season of the ark shell was during the months of July and August, and the gonads were in inactive stage during the winter. The gonad-visceral mass contained lower amounts of proteins than the other body parts. The most marked changes in body composition were lipids and carbohydrates within the gonad-visceral mass, and protein for each of the organs was relatively consistent throughout the year. All the parts in the visceral sac displayed the highest changes during the gametogenic cycle while the contents of moisture and lipid within the visceral act displayed somewhat inverse relations with each other. Moisture content was the lowest during the inactive stage during which the lipid content is the highest. The lipid content was the lowest immediately following spawning with increase in the moisture content as the lipid is being consumed. Protein mass within the visceral sac was low in comparison to the muscle mass. It is deemed that carbohydrates, lipids and proteins in the visceral sac play the major role as the source of energy during the development process of the gonads, and used for maintenance of base metabolism when available food is scarce.

• Journal of Nutrition and Health
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• v.36 no.6
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• pp.577-588
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• 2003

Severe protein-calorie malnutrition, common in patients with advanced liver disease, can seriously undermine the capacity for regeneration and functional restoration of liver. Nutritional supplementation for these patients can improve biochemical and hormonal abnormalities. However, these effects were not identified in patient with nonalcoholic liver cirrhosis. To determine effects of nutritional supplementation in patients with nonalcoholic liver cirrhosis, 77 subjects aged 29 to 69 years participated in this study for 12 weeks and were subdivided into three groups; normal diet group (Control group, n ＝ 16), branched-chain amino acid supplementation group (BCAA group, n ＝ 31), nutritional supplementation group (NS group, n ＝ 30). Anthropometric parameters, hemoglobin, hematocrit, blood cell counts, serum levels of lipids, vitamins, minerals and fatty acid composition, and plasma amino acids were examined. The mean values of age and height, and the initial values of weight and body mass index (BMI) were not different among all groups. After 12 weeks, there were no significant changes in these values in Control group. Only NS group showed significant increases in weight, lean body mass, midarm circumference, triceps skinfold thickness. Serum transferrins were increased both in BCAA and NS groups. Plasma levels of branched-chain amino acids, urea amino acids and glutamic acid were also significantly increased in these groups, but plasma levels of ammonia, serum LDL cholesterol and atherogenic index were decreased. However, there were no significant changes in serum levels of vitamin and mineral and composition of fatty acids in phospholipids in these groups. These results showed that the nutritional supplementation for patients with nonalcoholic liver cirrhosis can more improve nutritional status in these people together with increases of weight, body fat and lean body mass, compared to only BCAA supplementation. To ascertain and investigate the appropriate nutritional supplementation for patients with nonalcoholic liver cirrhosis, further studies are necessary.

• Journal of radiological science and technology
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• v.41 no.5
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• pp.463-469
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• 2018

Morphological information such as shape and margin of micro lesion is important information for diagnosis of disease in clinical imaging. In this study, we investigated the morphological changes of the micro lesions by comparing the contrast and area in grid suppressed DR images according to grid frequency. In the profile analysis of the image, the mass showed an average intensity variation of 8.6 ~ 72.4 after suppression, The higher the grid frequency, the more the contrast was increased. However, in the images obtained using 103 lp / inch, which is a grid frequency less than the sampling frequency, the contrast of the mass in the vertical direction decreased after suppression. In the binary image, the area change of the mass was also large. As a result, the shape, size, and margin of the mass changed. In the case of very small calcification, the higher the grid frequency is the larger the change in contrast, so that a clear image can be obtained in the post-suppression image. However, we could confirm that the margin of the lesion was blurred and the lesion was lost in some of the images using the 103 lp / inch grid. The higher the frequency of the grid, The change of the contrast of fiber occurred largely and clear boundary was confirmed. The decrease of the number of pixels was small and morphological change was small. In conclusion, when using a grid frequency that is not suitable for the sample frequency, morphological changes or lesion loss of micro lesions in the post- suppression image may give the possibility of misdiagnosis in diagnosis and differentiation of the image.

• Journal of Microbiology and Biotechnology
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• v.22 no.11
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• pp.1523-1531
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• 2012

The metabolite profile of meju during fermentation was analyzed using mass spectrometry techniques, including GC-MS and LC-MS, and the bacterial diversity was characterized. The relative proportions of bacterial strains indicated that lactic acid bacteria, such as Enterococcus faecium and Leuconostoc lactis, were the dominant species. In partial least-squares discriminate analysis (PLS-DA), the componential changes, which depended on fermentation, proceeded gradually in both the GC-MS and LC-MS data sets. During fermentation, lactic acid, amino acids, monosaccharides, sugar alcohols, and isoflavonoid aglycones (daidzein and genistein) increased, whereas citric acid, glucosides, and disaccharides decreased. MS-based metabolite profiling and bacterial diversity characterization of meju demonstrated the changes in metabolites according to the fermentation period and provided a better understanding of the correlation between metabolites and bacterial diversity.

• The Korean Journal of Ecology
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• v.23 no.2
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• pp.157-161
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• 2000

Mass loss and changes of mineral nutrients during decomposition of Phragmites communis for 13 months from November 1998 to December 1999, were investigated at the fringe of stream at Boryeong, Chungnam Province in Korea. Plant materials, which were collected in November 1998. were divided into leaves, culms and rhizomes. Litterbags, 15${\times}$15 cm, were made of nylon mesh with 2-mm$^2$ holes. At 13 months after installation, remaining mass of leaves, culms and rhizomes was 29.0%, 57.4%, 20.6%, respectively. Mass loss rate of the culms was significantly lower than those of the leaves and rhizomes. The decay rate of leaves, culms and rhizomes was 1.21. 0.42 and 1.48 per year, respectively. Initial concentration of N, P, K, Ca and Mg of leaves. culms and rhizomes was 22.5, 9.0, 15.5 mg/g for N, 0.34. 0.10, 0.33 mg/g for P, 15.0, 12.5. 12.3 mg/g for K, 2.84. 0.80, 0.03 mg/g for Ca. 1.94. 0.97, 0.40 mg/g for Mg, respectively. Concentrations of nutrients were higher in leaves than in culms and rhizomes. Except for N and Mg in rhizomes, there was no immobilization period during the decomposition. In the case of remaining K and Ca, most are lost during the first 3 months. Without any suitable method for removal of dead part, eutrophication of freshwater may be accelerated by dead macrophytes.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.3
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• pp.402-410
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• 2000

As the combustion process of CWM consists of the water evaporation, the release and combustion of volatile matter, and the combustion of char for every particle, it is more complex than that of existent liquid fuel. Though the many studies on CWM combustion have been carried out by the single droplet using hanging methods or the multiple droplet using atomization methods, any report don't presents definite solution about the effects by the initial water evaporation and combustion of volatile. When CWM is suddenly exposed in the high temperature surroundings, the internal water evaporates and then each droplet builds up pores. Besides, porosity rate changes along the temperature of surroundings, the composition ratio of CWM, and the initial diameter of droplet. In result, because it affects the whole combustion rate, the combustion of CWM has complex mechanism as compared with the combustion of liquid or gas fuel. Therefore, concentrating on porous structure of CWM, this study has proceeded to acquire the basic data on the CWM injection combustion and closely examines the effects of the first stage combustion on the whole combustion by measuring the diameter variations, pore rate, mass fraction burned, and the internal temperature changes of CWM droplet. The results demonstrate that $60{\sim}70%$ of initial mass is reduced during water evaporation and volatile combustion period, and swelling rate, mass faction burned, and density variation are greatly concerned with atomization of CWM etc.

• Korean Journal of Applied Biomechanics
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• v.25 no.3
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• pp.257-263
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• 2015

Objective : The purpose of this study was to investigate the effect of genu valgum on the body mass index, movement of lower limb joints, and ground reaction force. Methods : Gait patterns of 30 college students with genu valgum were analyzed and the static Q angle of the femur was measured for selecting genu valgum of the subjects. To analyze the kinetic changes during walking, the six-camera Vicon MX motion analysis system was used. The subjects were asked to walk 12 meters using the more comfortable walking method for walking. After they walked 12 meters more than 10 times, their most natural walking patterns were chosen three times and analyzed. Results : As a result of measuring a relationship between genu valgum and Q-angle, as the Q-angle increases, it showed a genu valgum also increased. Body Mass Index showed a significant difference between the groups was higher in the genu valgum group.(p<.001). The analysis result showed that genu valgum had a significant effect on the internal rotation moment in the hip joint(p<.05). Also, genu valgum had a significant effect on the internal rotation moment of the knee joint(p<.05). The comparative analysis of the Medial-Lateral ground reaction force in the genu valgum group showed a tendency to increase the medial ground reaction force(p<.05). The vertical ground reaction forces of the middle of the stance phase(Fz0) showed a significant increase in genu valgum group(p<.05), in particular the results showed a decrease in the early stance phase(p<.001). Conclusion : In conclusion, the change in body mass is considered to be made by proactive regular exercise for improvement of the genu valgum. In addition, the prevention of the deformation caused by secondary of the genu valgum in this study may be used as an indicator of the position alignment rehabilitation for structural and functional improvements. Applying a therapeutic exercise program for the next lap will require changes in posture alignment.