• Macromolecular Research
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• 제11권5호
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• pp.341-346
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• 2003

A poly(ethylene oxide)-b-poly(L-lactide) diblock copolymer (PEO-b-PLLA) is characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and a block length distribution map is constructed. Although the MALDI- TOF mass spectrum of PEO-b-PLLA is very complicated, most of the polymer species were identified by isolating the overlapped isotope patterns and by fitting the overlapped peaks to the Schulz-Zimm distribution function. Reconstructed MALDI-TOF MS spectrum was nearly identical to the measured spectrum and this method shows its potential to be developed as an easy and fast analysis method of low molecular weight block copolymers.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 추계학술대회논문집A
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• pp.465-470
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• 2001

In this study, the seismic analysis of rack structure with fluid-structure interaction is performed through use of the Finite Element Method(FEM) code ANSYS. Fluid-structure interaction can specify in terms of an hydrodynamic effect which is defined as the added mass per unit length divided by the area of the cross section. Using the Floor Response Spectrum(FRS) obtained through the time-history analysis, modal analysis and seismic analysis under Operating Basis Earthquake(OBE) and Safe Shutdown Earthquake(SSE) condition is carried out. The fluid-structure interaction effects on the rack structure are investigated.

• Earthquakes and Structures
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• 제7권4호
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• pp.587-607
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• 2014

Progressive collapse, which is referred to as the collapse of the entire building under local damages, is a common failure mode happened by earthquakes. The collapse process highly depends on the whole structural system. Since, asymmetry of the building plan leads to the local damage concentration; it may intensify the progressive collapse mechanism of asymmetric buildings. In this research the progressive collapse of regular and irregular 6-story RC ordinary moment resisting frame buildings are studied in the presence of the earthquake loads. Collapse process and collapse propagation are investigated using nonlinear time history analyses (NLTHA) in buildings with 5%, 15% and 25% mass asymmetry with respect to the number of collapsed hinges and story drifts criteria. Results show that increasing the value of mass eccentricity makes the asymmetric buildings become unstable earlier and in the early stages with lower number of the collapsed hinges. So, with increasing the mass eccentricity in building, instability and collapse of the entire building occurs earlier, with lower potential of the progressive collapse. It is also demonstrated that with increasing the mass asymmetry the decreasing trend of the number of collapsed beam and column hinges is approximately similar to the decreasing trend in the average story drifts of the mass centers and stiff edges. So, as an alternative to a much difficult-to-calculate local response parameter of the number of collapsed hinges, the story drift, as a global response parameter, measures the potential of progressive collapse more easily.

• Mass Spectrometry Letters
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• 제13권4호
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• pp.177-183
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• 2022

The monomer and dimer structures of the amyloid fragment Aβ(1-16) sequence formed in H₂O were investigated using electrospray ionization mass spectrometry (MS) and tandem MS (MS/MS). Aβ16 monomers and dimers were indicated by signals representing multiple proton adduct forms, [monomer+zH]ⁿ⁺ (=M^z+, z = charge state) and [dimer+zH]^z+ (=D^z+), in the MS spectrum. Fragment ions of monomers and dimers were observed using collision-induced dissociation MS/MS. Peptide bond dissociation was mostly observed in the D1-D7 and V11-K16 regions of the MS/MS spectra for the monomer (or dimer), regardless of the monomer (or dimer) charge state. Both covalent and non-covalent bond dissociation processes were indicated by the MS/MS results for the dimers. During the non-covalent bond dissociation process, the D³⁺ dimer complex was separated into two components: the M¹⁺ and M²⁺ subunits. During the covalent bond dissociation of the D³⁺ dimer complex, the b and y fragment ions attached to the monomer, (M+b_10-15)^z+ and (M+y_9-15)^z+, were thought to originate from the dissociation of the M²⁺ monomer component of the (M¹⁺+M²⁺) complex. Two different D³⁺ complex geometries exist; two distinguished interaction geometries resulting from interactions between the M¹⁺ monomer and two different regions of M²⁺ (the N-terminus and C-terminus) are proposed. Intricate fragmentation patterns were observed in the MS/MS spectrum of the D⁵⁺ complex. The complicated nature of the MS/MS spectrum is attributable to the coexistence of two D⁵⁺ configurations, (M¹⁺+M⁴⁺) and (M²⁺M³⁺), in the Aβ16 solution.

• 한국압력기기공학회 논문집
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• 제16권1호
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• pp.30-36
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• 2020

In SMART, the flow mixing header assembly (FMHA) is used to mix the coolant flowing into the reactor core to maintain a uniform temperature. The FMHA is designed to have enough stiffness so the resonance with reactor internal structures does not occurs during the pipe break and the seismic accidents. Since the gap between the FMHA and the core support barrel assembly is very narrow compared with the diameter of FMHA, the hydrodynamic mass effect acting on the FMHA is not negligible. Therefore the hydrodynamic mass characteristics on the FMHA are investigated to consider the fluid and structure interaction effects. The result of modal analysis for the dry and underwater conditions, the natural frequency of primary vibration mode for the horizontal direction is reduced from 136.67 Hz to 43.76 Hz. Also the result of frequency response spectrum seismic analysis for the dry and underwater conditions, the maximum equivalent stress are increased from 13.89 MPa to 40.23 MPa. Therefore, reactor internal structures located in underwater condition shall consider carefully the hydrodynamic mass effects even though they have sufficient stiffness required for performing its functions under the dry condition.

• 대한소아치과학회지
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• 제30권3호
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• pp.530-539
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• 2003

본 연구에서는 4종의 복합레진(Palpique Estelite, Filtek P 60, Spectrum, Charisma)을 선택하였다. 각 제품당 5개의 시편을 제작하여 무게 측정을 한 후, NaOH 용액에 2주 동안 보관하였고 1.23% HCl로 중화하고 세척, 건조시켜 무게 측정을 하였다. 주사전자현미경과 공초점 레이저 주사현미경으로 복합레진의 분해층 표면과 분해깊이를 관찰하였고, 분해 저항성은 용액내로 용출된 Si, Al, Ba의 농도를 근거로 평가하여 다음과 같은 결과를 얻었다. 1. 각 제품의 무게손실량은 Palpique Estelite가 가장 많았고, Filtek P 60, Charisma, Spectrum 순이었다. 2. 각 제품의 표면하 분해층 깊이는 Filtek P 60이 가장 깊었고, Charisma, Palpique Estelite, Spectrum 순이었다. 3. Si 용출량은 Charisma가 가장 많았고, Spectrum, Palpique Estelite, Filtek P 60 순이었다. 4. 무게손실과 분해층 깊이 사이에는 높은 상관관계를 보였다(r=0.704, p<0.05). 5. 주사전자현미경 관찰시 NaOH용액에 보관한 후 레진 기질과 충전제 사이의 결합의 파괴 양상인 분해층 표면을 관찰할 수 있었고 공초점 레이저 현미경 관찰시 레진의 분해층 깊이를 관찰할 수 있었다.

• Structural Engineering and Mechanics
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• 제43권4호
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• pp.527-543
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• 2012

This paper presents a response spectrum analysis procedure suitable for base isolated regular buildings subjected to near fault ground motions. This procedure is based on the fact that the isolation system may be treated separately since the superstructure behaves as a rigid body on well selected isolation systems. The base isolated building is decomposed into several single-degree of freedom systems, the first one having the total weight of the building is isolated while the remainder when superposed they replicate approximately the behavior of the superstructure. The response of the isolation system is governed by a response spectrum generated for a single isolated mass. The concept of the procedure and its application for the analysis of base isolated structures is illustrated with an example. The present analysis procedure is shown to be accurate enough for the preliminary design and overcomes the limits of applicability of the conventional linear response spectrum analysis.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2009년도 추계학술발표대회 논문집
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• pp.86-91
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• 2009

In this paper, we analyze the Transition of Isc by natural solar spectrum of c-Si and a-Si PV module. Commonly, performance of photovoltaic (PV) module is estimated under the standard test condition (STC). That is, solar irradiance $1kW/m^2$, solar spectrum distribution: AM1 5G, module temperature $25^{\circ}C$ This means it rarely meets actual outdoor conditions. The solar spectrum always changes. So it is rare to fit the standard solar spectrum AM1 5G defined in ASTM G173-03 or IEC 60904-3. Thus spectral response of PV module is different depending on the material. so we estimated the variation of Isc at every minutes by comparing c-Si PV module with a-si PV module for outdoor conditions.

• 소음진동
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• 제1권2호
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• pp.115-120
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• 1991

The impact hammer is extensively used in experimental modal analysis as a means to provide force over a broad range of frequencies. The hammer mass and the impact head are often changed to achieve a desired impact time duration with its corresponding input frequency spectrum, these mass changes affect the performance and sensitivity of the force transducer employed to measure the impact force. Both a mathematical model describing the effects of impact head and hammer mass on the performance of the force transducer and experimental verification of this model are presented here.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1054-1059
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• 2005

An extracellular protease was identified from Bacillus subtilis KCCM 10257 by N-terminal sequencing and mass spectral analysis. The molecular mass of the extracellular protease was estimated to be 28 kDa by SDS-PAGE. Sequencing of the N-terminal of the protease revealed the sequence of A(G,S,R)QXVPYG(A)V(P,L)SQ. The N-terminal sequence exhibited close similarity to the sequence of other proteases from Bacillus sp. A mass list of the monoisotopic peaks in the MALDI-TOF spectrum was searched after peptide fragmentation of the protease. Six peptide sequences exhibiting monoisotopic masses of 1,276.61, 1,513.67, 1,652.81, 1,661.83, 1,252.61, and 1,033.46 were observed from the fragmented protease. These monisotopic masses corresponded to the lytic enzyme L27 from Bacillus subtilis 168, and the Mowse score was found to be 75. A doubly charged Top product (MS) at a m/z of 517.3 exhibiting a molecular mass of 1034.6 was further analyzed by de novo sequencing using a PE Sciex QSTAR Hybrid Quadropole-TOF (MS/MS) mass spectrometer. MS/MS spectra of the Top product (MS) at a m/z of 517.3 obtained from the fragmented peptide mixture of protease with Q-star contained the b-ion series of 114.2, 171.2, 286.2, 357.2, 504.2, 667.4, 830.1, and 887.1 and y-ion series of 147.5, 204.2, 367.2, 530.3, 677.4, 748.4, 863.4, and 920.5. The sequence of analyzed peptide ion was identified as LGDAFYYG from the b- and y-ion series by de novo sequencing and corresponded to the results from the MALDI-TOF spectrum. From these results the extracellular protease from Bacillus subtilis KCCM 10257 was successfully identified with the lytic enzyme L27 from Bacillus subtilis 168.