• Interdisciplinary Bio Central
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• 제1권3호
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• pp.9.1-9.6
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• 2009

Introduction: In the mass spectrometry-based proteomics, biological samples are analyzed to identify proteins by mass spectrometer and database search. Database search is the process to select the best matches to the experimental mass spectra among the amino acid sequence database and we identify the protein as the matched sequence. The match score is defined to find the matches from the database and declare the highest scored hit as the most probable protein. According to the score definition, search result varies. In this study, the difference among search results of different search engines or different databases was investigated, in order to suggest a better way to identify more proteins with higher reliability. Materials and Methods: The protein extract of human mesenchymal stem cell was separated by several bands by one-dimensional electrophorysis. One-dimensional gel was excised one by one, digested by trypsin and analyzed by a mass spectrometer, FT LTQ. The tandem mass (MS/MS) spectra of peptide ions were applied to the database search of X!Tandem, Mascot and Sequest search engines with IPI human database and SwissProt database. The search result was filtered by several threshold probability values of the Trans-Proteomic Pipeline (TPP) of the Institute for Systems Biology. The analysis of the output which was generated from TPP was performed. Results and Discussion: For each MS/MS spectrum, the peptide sequences which were identified from different conditions such as search engines, threshold probability, and sequence database were compared. The main difference of peptide identification at high threshold probability was caused by not the difference of sequence database but the difference of the score. As the threshold probability decreases, the missed peptides appeared. Conversely, in the extremely high threshold level, we missed many true assignments. Conclusion and Prospects: The different identification result of the search engines was mainly caused by the different scoring algorithms. Usually in proteomics high-scored peptides are selected and low-scored peptides are discarded. Many of them are true negatives. By integrating the search results from different parameter and different search engines, the protein identification process can be improved.

• 한국심리학회지 : 문화 및 사회문제
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• 제11권3호
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• pp.67-89
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• 2005

본 연구에서는 대중문화를 학문적 관점에서 개관해 보고 이를 바탕으로 대중문화에 대한 심리학적 접근을 탐색해 보았다. 지금까지 이루어진 대중문화 연구를 연구대상에 따라 살펴 보았으며, 산업과 소비자학, 교육 및 발달심리학의 관점에서도 분석해 보았다. 이와 더불어 대중문화를 바라보는 각각의 관점에 따라 어떻게 연구의 경향과 한계점이 나타나는지도 논의해 보았다. 본 연구는 이러한 분석을 바탕으로 대중문화 연구에 대한 전반적인 심리학적 관심의 촉구, 대중문화에 대한 정서적 체험의 분석과 측정의 필요성, 기획 문화적 관점에서 산업 및 소비자학적 접근의 역할 증대, 청소년 문화를 긍정적으로 수용하는 발달심리학적 시각의 변화, 대중문화 관련 학제 간 연구 등을 제안하고 있다. 이미 대중문화는 우리 실생활에 밀접하게 침투되어 있으나 이를 해석하고 분석하는 일은 심리학적 관점에서 보면 매우 미미하였다. 본 연구는 우리 생활에 함께하는 대중문화에 대한 관심과 그 실체를 인정하는 입장에서 대중문화 논의를 시도하였다는데 의미가 있으며, 본 연구를 통하여 심리학의 관심 영역이 확대되고 대중문화에 대한 접근 방법이 다양화되었으면 한다.

• Mass Spectrometry Letters
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• 제4권3호
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• pp.41-46
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• 2013

The goal of this study is to find an experimental condition which enables us to perform enzymatic studies on the cellular behavior of PTEN (phosphatase and tensine homolog) through identification of molecular species of phosphatidylinositol 3,4,5-trisphosphates and their quantitative analysis in a mammalian cell line using mass spectrometry. We initially exployed a two-step extraction process using HCl for extraction of phosphatidylinositol 3,4,5-trisphosphates from two mammalian cell lines and further analyzed the extracted phosphatidylinositol 3,4,5-trisphosphates using tandem mass spectrometry for the identification of them. We finally quantified the concentration of phosphatidylinositol 3,4,5-trisphosphates using internal standard calibration. From these observation, we found that HEK 293-T cells is a good model to examine the enzymatic behavior of PTEN in a cell, and the minimum amount of phosphatidylinositol 3,4,5-trisphosphates is more than 50 pmol for quantification in a mass spectrometer. These results suggest that the well-optimized experimental conditions are required for the investigation of the cellular PTEN in terms of the catalytic mechanism and further for the detailed identification of cellular substrates.

• Tribology and Lubricants
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• 제30권4호
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• pp.240-245
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• 2014

Low alloy heat resistant brake disk and sintered brake pad are applied to mechanical brake system for the speed-up of urban rapid transit. In this research, we analyzed how the frictional characteristics between brake disk and pad are influenced by the disk mass. At a high disk mass, the friction stability was the lower value as a result of the lack of tribofilm formation at the disk surface. Wear rates of friction materials showed the higher value at a low disk mass and wear rates of 10 mm and 15 mm showed the similar level. Average friction coefficient was the lower value at the 10 mm disk thickness and range of variation of average friction coefficient was also the smaller value at the 10 mm disk thickness. However, there were no significant changes in the friction coefficients under any of test conditions. Surface roughness of a disk showed the highest value at the 5 mm disk and surface roughnesses of 10 mm and 15 mm showed the similar level. As a result, friction characteristics of disk mass influenced the friction stability, as well as the wear rate of friction pad and disk, but not the friction coefficient.

• 대한기계학회논문집A
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• 제23권11호
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• pp.1940-1951
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• 1999

In this paper, the equations of motion of a Bernoulli-Euler cantilever beam fixed on a moving cart and carrying a lumped mass concentrated at an arbitrary position along the beam is derived. The motion of the beam-mass-cart system is analyzed through unconstrained modal analysis, and a unified characteristic equation for calculating the natural frequencies of the system is obtained. The changes of natural frequencies and the corresponding mode shapes with respect to the changes in mass ratios of the system and to the concentrated position of the lumped mass are investigated with the frequency equation, which can be generally applied to this kind of systems. The exact and assumed-mode solutions including the dynamics of the base cart are obtained, and the open-loop responses of the system by arbitrarily designed forcing function are given by numerical simulations. The results match well with physical phenomena even at the extreme cases where the concentrated mass is attached to the bottom and to the top of the beam.

• Bulletin of the Korean Chemical Society
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• 제26권2호
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• pp.303-308
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• 2005

A miniaturized time-of-flight mass spectrometer with an electron impact ionization source and sheet membrane introduction has been developed. The advantages and features of this mass spectrometer include high sensitivity, simple structure, low cost, compact volume with field portability, and ease of operation. A mass resolution of 400 at m/z 78 has been obtained with a 25 cm flight path length. Under optimized conditions, the detection limits for the volatile organic compounds (VOCs) studied were 0.2-10 ppm by volume with linear dynamic ranges greater than three orders of magnitude. The response times for various VOCs using a silicone membrane of 127 $\mu$m thickness were in the range 4.5-20 s, which provides a sample analysis time of less than 1 minute. These results indicate that the membrane introduction/time-of-flight mass spectrometer will be useful for a wide range of field applications, particularly for environmental monitoring.

• 대한불안의학회지
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• 제8권2호
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• pp.71-78
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• 2012

The mass social trauma, such as organized violence, wars, oppression by dictatorships and massive terrorist attacks, exposes thousands of people to trauma in a short period of time. Therefore, the mass social trauma is distinguished from individualized trauma, such as a violent attack, rape or a traffic accident in that it results in multiple and extended consequences beyond the individual. During the Khmer Rouge regime, one quarter of the Cambodian population was killed as a result of malnutrition, forced labor and mass killings. Until now, its evil continues to affect Cambodian's physical and mental health problems. Although there is ongoing debate, to date, no consensus has been reached supporting a clear set of recommendations for the intervention and longitudinal study regarding the influence of killing field massacre being too little. And comparative cultural studies, such as comparing the East to West or other Asian cultures are also lacking. This article gives an overview of previous study results about the mental health of Cambodians, and suggests a possible research issue and therapeutic interventions to determine the impact of mass trauma to the members of society and post-traumatic recovery factors.

• 약학회지
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• 제57권6호
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• pp.412-419
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• 2013

Drug discovery and development processes are time consuming and costly endeavors. It has been reported that on average it takes 10 to 15 years and costs more than $ 1billion to bring a molecule from discovery to market. Compounds fail for various reasons but one of the significant reasons that accounts for failures in clinical trials is poor prediction/understanding of pharmacokinetics and drug metabolism in human. In an effort to improve the number of compounds that exhibit optimal absorption, distribution, metabolism, elimination (ADME), and pharmacokinetic properties in human, drug metabolism, pharmacokinetic scientists have been continually developing new technologies and compound screening strategies. Over the last few years, accelerator mass spectrometry (AMS) and its applications to preclinical/clinical pharmacokinetics and ADME studies have significantly increased, particularly for new chemical/biological entities that are difficult to support with conventional radiolabel studies. In this review, the application of AMS for micro-dosing, micro-tracer absolute bioavailability, mass balance and metabolite profiling studies will be discussed.

• 천문학회보
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• 제46권2호
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• pp.69.2-69.2
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• 2021

We present the discovery of rare active galactic nuclei (AGNs) in nearby (z<0.05) compact elliptical galaxies (cEs) located in isolated environments. Using spectroscopic data from the Sloan Digital Sky Survey (SDSS) Data Release 12, four AGNs were identified based on the optical emission-line diagnostic diagram. SDSS optical spectra of AGNs show the presence of distinct narrow-line emissions. Utilizing the black hole (BH) mass-stellar velocity dispersion scaling relation and the correlation between the narrow L([OIII])/L(Hβ) line ratio and the width of the broad Hα emission line, we estimated the BH masses of the cEs to be in the range of 7×10⁵-8×10⁷ solar mass. The observed surface brightness profiles of the cEs were fitted with a double Sérsic function using the Dark Energy Camera Legacy Survey r-band imaging data. Assuming the inner component as the bulge, the K-band bulge luminosity was also estimated from the corresponding Two Micron All Sky Survey images. We found that our cEs follow the observed BH mass-stellar velocity dispersion and BH mass-bulge luminosity scaling relations, albeit there was a large uncertainty in the derived BH mass of one cE. In view of the observational properties of BHs and those of the stellar populations of cEs, we discuss the proposition that cEs in isolated environments are bona fide low-mass early-type galaxies (i.e., a nature origin).

• Journal of Applied Biological Chemistry
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• 제56권1호
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• pp.53-57
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• 2013

비료제품에는 의도적 혹은 비의도적으로 농약으로 등록된 indole-3-acetic acid (IAA) 및 indole-3-butyric acid (IBA)성분에 대한 잔류 가능성이 꾸준히 제기되고 있으나, 생장조정제가 아닌 비료제품에서 mg/L 수준의 옥신류 잔류분석법이 마련되어 있지 않았다. 따라서, 본 연구에서는 생체시료에 미량 잔류하는 IAA 및 IBA 분석에 사용되어 온 liquid chromatography-mass/mass spectrometry (LC-MS/MS)와 gas chromatography-MS 기기분석법을 활용하여 농축 액상비료제품에 적용할 수 있는지 조사하였으며, 액상 비료제품에 적용 가능한 정밀기기 분석법을 개발하고자 하였다. 수용액 상태의 시료에서 식물 생장호르몬인 IAA와 IBA를 가장 손쉽게 정제할 수 있는 방법으로 hydrophile-lipophile balance (HLB) solid phase extraction를 활용하였으나, 제한된 조건에서 LC-MS/MS를 통한 정량분석은 적합한 회수율을 확보하지 못하였고, 정성분석만 가능함을 확인하였다. 반면, 비료제품 250배 희석액을 사용하여 HLB 정제, trimethylsilyl chloride을 이용한 methylation을 통한 GC-MS 분석에서는 검출한계 1.4 mg/L과 93-107%의 회수율로 액상 비료제품에서 IAA와 IBA 정량분석법을 확립할 수 있었다.