• Title/Summary/Keyword: Marker-set

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Verification of X-sight Lung Tracking System in the CyberKnife (사이버나이프에서 폐종양 추적 시스템의 정확도 분석)

  • Huh, Hyun-Do;Choi, Sang-Hyoun;Kim, Woo-Chul;Kim, Hun-Jeong;Kim, Seong-Hoon;Cho, Sam-Ju;Min, Chul-Ki;Cho, Kwang-Hwan;Lee, Sang-Hoon;Choi, Jin-Ho;Lim, Sang-Wook;Shin, Dong-Oh
    • Progress in Medical Physics
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    • v.20 no.3
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    • pp.174-179
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    • 2009
  • To track moving tumor in real time, CyberKnife system imports a technique of the synchrony respiratory tracking system. The fiducial marker which are detectable in X-ray images were demand in CyberKnife Robotic radiosurgery system. It issued as reference markers to locate and track tumor location during patient alignment and treatment delivery. Fiducial marker implantation is an invasive surgical operation that carries a relatively high risk of pneumothorax. Most recently, it was developed a direct lung tumor registration method that does not require the use of fiducials. The purpose of this study is to measure the accuracy of target applying X-sight lung tracking using the Gafchromic film in dynamic moving thorax phantom. The X-sight Lung Tracking quality assurance motion phantom simulates simple respiratory motion of a lung tumor and provides Gafchromic dosimetry film-based test capability at locations inside the phantom corresponding to a typical lung tumor. The total average error for the X-sight Lung Tracking System with a moving target was $0.85{\pm}0.22$ mm. The results were considered reliable and applicable for lung tumor treatment in CyberKnife radiosurgery system. Clinically, breathing patterns of patients may vary during radiation therapy. Therefore, additional studies with a set real patient data are necessary to evaluate the target accuracy for the X-sight Lung Tracking system.

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Use of Microsatellite Markers to Identify Commercial Melon Cultivars and for Hybrid Seed Purity Testing (Microsatellite Marker를 이용한 멜론 시판품종의 품종식별과 F1 순도검정)

  • Kwon, Yong-Sham;Hong, Jee-Hwa
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.525-534
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    • 2014
  • Microsatellite markers were used to identify 58 major commercial melon cultivars, and to assess hybrid seed purity of a melon breeding line known as '10H08'. A set of 412 microsatellite primer pairs were utilized for fingerprinting of the melon cultivars. Twenty-nine markers showed hyper-variability and could discriminate all cultivars on the basis of marker genotypes, representing the genetic variation within varietal groups. Cluster analysis based on Jaccard's distance coefficients using the UPGMA algorithm categorized 2 major groups, which were in accordance to morphological traits. The DNA bulks of female and male parents of breeding line '10H08' were tested with 29 primer pairs based on microsatellites to investigate purity testing of $F_1$ hybrid seeds, and 5 primer pairs exhibited polymorphism. One microsatellite primer pair (CMGAN12) produced unambiguous polymorphic bands among the parents. Among 192 seeds tested with CMGAN12, progeny possibly generated by self-pollination of the female parent were clearly distinguished from the hybrid progeny. These markers will be useful for fingerprinting melon cultivars and can help private seed companies to improve melon seed purity.

A Study on Function of the Delineation System by Pattern for Safety Audit on Road Exit Ramp (국도유출부 안전진단을 위한 시선유도시설의 유형별 기능검토)

  • Kum, Ki-Jung;Kim, Hong-Sang;Min, Kyeong-Tae;Yang, Gye-Seung
    • International Journal of Highway Engineering
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    • v.8 no.4 s.30
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    • pp.1-11
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    • 2006
  • Currently, road mobility improved from the National Road Improvement. Nevertheless delineation system is facility that enhanced driver's safety, that was set up often inconsistent or nonexistent over the road exit ramp So it judged functional investigation will be necessary. This study suggested setting type of the delineation system. That was based on a field study and reviews the legal standard of it and considering driver's cognition behavior. For the study, make a 3D-simulation and so could objectively a comparative test. Comparison variable between delineation setting type is selected conspicuity and visibility. Cased that illustrated characteristics of driver's visual cognition behavior. The experiment was used Eye Marker Recorder for measure the gaze frequency more quantitatively and objectively. And used the ANOVA analysis for significance testing between delineation setting type. A significant percent of the conspicuity analyzed types(Safe mark, Obstacle Sign, Warning Light, and Tubular Maker) in road exit ramp for recognize. And gaze frequency that measure of effectiveness of visibility are measured. On the analysis result, the visibility was significance difference between delineation setting type and visibility of types was best.

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Construction of a Microsatellite DNA Profile Database for Pear Cultivars and Germplasm (배 품종 및 유전자원에 대한 Microsatellite DNA 프로파일 데이터베이스 구축)

  • Hong, Jee-Hwa;Shim, Eun-Jo;Kwon, Yong-Sham
    • Horticultural Science & Technology
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    • v.35 no.1
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    • pp.98-107
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    • 2017
  • A DNA profile database was constructed to investigate the genetic relatedness of 72 germplasm samples of Pyrus and related cultivars using microsatellite markers. Three P. pyrifolia, four P. commus, and one P. betulifolia cultivars with different morphological traits were screened using 387 pairs of microsatellite primers. A core set of 11 primer pairs was selected to obtain 133 polymorphic amplified fragments meeting three criteria: high polymorphism information contents (PIC), high repeatability, and distinct allele patterns. The number of alleles per locus ranged between 4 and 22. Average PIC was 0.743 (range: 0.557 - 0.879). Cluster analysis using the unweighted pair - group method with arithmetical average (UPGMA) separated the 72 pear cultivars and germplasm samples into four major groups: Chinese, European pears, and a cluster of 55 Asian pears that could be reclassify into two subcluster, I - $1^{st}$ and II - $2^{nd}$, according to pedigree information. Almost all of the cultivars were discriminated by 11 microsatellite marker genotypes. The microsatellite DNA profile database may be utilized as tool to verify distinctness, uniformity, and stability between candidate cultivar, and to verify in the distinctness of existing cultivars.

Efficient Local Decoding Using Bit Stream Map for High Resolution Video (비트 스트림 지도를 이용한 고해상도 영상의 효율적인 지역복호화)

  • Park Sungwon;Won Jongwoo;Lee Sunyoung;Kim Wookjoong;Kim Kyuheon;Jang Euee S
    • Journal of Broadcast Engineering
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    • v.9 no.4 s.25
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    • pp.391-401
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    • 2004
  • In this paper, we introduce a novel coding method to efficiently enable spatial random access for high resolution video. In terms of resolution and display size, standard display devices (such as cathode-ray tubes. monitors. PDAs, and LCDs) do not sufficiently support high resolution video such as digital cinema and panoramic video. Currently, users have no choice but to view video at lower resolution as a result of down-sampling, or only a partial region of the video due to display size limitations. Our proposed method. which we call the B-map, represents the set of starting locations of the coded segments in a picture frame. This information, or B-map, is first sent to the decoder prior to the coded data stream of the frame and is then used for fast local decoding. To test our method, we compare our B-map with JPEG tiling and the JPEG Resynchronization marker. Experimental results show that the proposed coding method requires less overhead than existing methods during the same decoding time. The results show promise for future panoramic or digital cinema applications.

Identification of Pleurotus ostreatus cultivars with the application of multiplex-simple sequence repeat markers (Multiplex SSR마커를 이용한 느타리(Pleurotus ostreatus) 품종 판별)

  • Choi, Jong In;Jung, Hwa Jin;Na, Kyeong sook;Oh, Min-Ji;Kim, Min-Keun;Ryu, Jae-San
    • Journal of Mushroom
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    • v.19 no.1
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    • pp.76-80
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    • 2021
  • To develop a method for the differentiation of Pleurotus ostratus cultivars, the multiplex-simple sequence repeat (SSR) primer set based on the SSRs obtained from whole genomic DNA sequence analysis was designed with two polymerase chain reaction (PCR) primer sets. These SSR primer sets were employed to distinguish 10 cultivars and strains. Twenty polymorphic markers were selected based on the genotyping results. PCR with each primer produced 1-4 distinct bands ranging in size from 150 to 350 bp, which was within the expected range. However, since a sole SSR marker was unable to detect polymorphisms in every cultivar, multiplex PCRs with composite PCR primer sets were employed. The multiplex primer, "166+115," completely discriminated 12 cultivars and strains with 40 loci, which were 12 more than the simple arithmetic addition of each locus of the primers 115 and 166. These results might be useful to provide an efficient method for the differentiation of P. ostreatus cultivars with separate PCRs for the quality control of spawn and protection of breeders' rights.

Development of Chloroplast Genome-based Insertion/Deletion Markers in the Genus Broussonetia (닥나무 속 식물의 엽록체 유전체 기반 InDel 마커의 개발)

  • Eun Jee Lee;Yoon A Kim;Mi Sun Lee;Ju Hyeok Kim;Young Kyu Choi;Jung Sung Kim;Chang Seob Sin;Yi Lee
    • Korean Journal of Plant Resources
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    • v.36 no.4
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    • pp.290-298
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    • 2023
  • Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of Korean paper known as Hanji. Three of these species, Broussonetia kazinoki, Broussonetia monoica, and Broussonetia papyrifera, are found in the Korean Peninsula. Because it is challenging to distinguish different Broussonetia species based on morphology alone, we have developed a set of insertion/deletion (InDel) markers for genetic identification of these species. From twenty-two Broussonetia samples collected throughout Korea, we selected six for next-generation sequencing analysis. InDel marker candidates were identified by comparing this sequence information with the B. kazinoki chloroplast genome sequence. The marker candidates were used to screen the genomes of the twenty-two Broussonetia plants, and five useful chloroplast-based InDel markers were identified. Detailed genotyping using these five markers showed that the twenty-two plants of the genus Broussonetia could be clustered into five groups, verifying that the markers developed here can be used for breeding, identification, and analysis of species in the genus Broussonetia.

A Design and Implementation of Health Schedule Application

  • Ji Woo Kim;Young Min Lee;Won Joo Lee
    • Journal of the Korea Society of Computer and Information
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    • v.29 no.3
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    • pp.99-106
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    • 2024
  • In this paper, we design and implement the HealthSchedule app, which records exercise data based on the GPS sensor embedded in smartphones. This app utilizes the smartphone's GPS sensor to collect real-time location information of the user and displays the movement path to the designated destination. It records the user's actual path using latitude and longitude coordinates. Users register exercise activities and destination points when scheduling, and initiate the exercise. When measuring the current location, a lime green departure marker is generated, and the movement path is displayed in blue, with the destination marker and a surrounding 25-meter radius circle shown in sky blue. Using the coordinates of the starting point or the previous location and the current GPS sensor-transmitted location coordinates, it measures the distance traveled, time taken, and calculates the speed. Furthermore, it accumulates measurement data to provide information on the total distance traveled, movement path, and overall average speed. Even when reaching the destination during exercise, the movement path continues to accumulate until the completion button is clicked. The completion button is activated when the user moves into the sky blue circular area with a radius of 25 meters, centered around the initially set destination. This means that the user must reach the designated destination, and if they wish to continue exercising without clicking the completion button, they can do so. Depending on the selected exercise type, the app displays the calories burned, aiming to increase user engagement and a sense of accomplishment.

Characterization of QTL for Growth and Meat Quality in Combined Pig QTL Populations

  • Li, Y.;Choi, B.H.;Lee, Y.M.;Alam, M.;Lee, J.H.;Kim, K.S.;Baek, K.H.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1651-1659
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    • 2011
  • This study was conducted to detect quantitative trait loci (QTL) for thirteen growth and meat quality traits in pigs by combing QTL experimental populations. Two F2 reference populations that were sired by Korea native pig (KNP) and dammed by Landrace (LN) or Yorkshire (YK) were generated to construct linkage maps using 123 genetic markers (mostly microsatellites) and to perform QTL analysis on porcine chromosomes (SSCs) 1, 2, 3, 6, 7, 8, 9, 11, 13, 14, and 15. A set of line-cross models was applied to detect QTL, and a series of lack-of-fit tests between the models was used to characterize inheritance mode of QTL. A total of 23, 11 and 19 QTL were detected at 5% chromosome-wise level for the data sets of KNP${\times}$LN, KNP${\times}$YK cross and joint sets of the two cross populations, respectively. With the joint data, two Mendelian expressed QTL for live weight and cooking loss were detected on SSC3 and SSC15 at 1% chromosome-wise level, respectively. Another Mendelian expressed QTL was detected for CIE a on SSC7 at 5% genome-wise level. Our results suggest that QTL analysis by combining data from two QTL populations increase power for QTL detection, which could provide more accurate genetic information in subsequent marker-assisted selection.

Detection of Mendelian and Parent-of-origin Quantitative Trait Loci for Meat Quality in a Cross between Korean Native Pig and Landrace

  • Choi, B.H.;Lee, Y.M.;Alam, M.;Lee, J.H.;Kim, T.H.;Kim, K.S.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1644-1650
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    • 2011
  • This study was conducted to detect quantitative trait loci (QTL) affecting meat quality in an $F_2$ reference population of Korean native pig and Landrace crossbreds. The three-generation mapping population was generated with 411 progeny from 38 $F_2$ full-sib families, and 133 genetic markers were used to produce a sex-average map of the 17 autosomes. The data set was analyzed using least squares Mendelian and parent-of-origin interval-mapping models. Lack-of-fit tests between models were used to characterize the QTL for mode of gene expressions. A total of 10 (32) QTL were detected at the 5% genome (chromosome)-wise level for the analyzed traits. Of the 42 QTL detected, 13 QTL were classified as Mendelian, 10 as paternal, 14 as maternal, and 5 as partial expressed QTL, respectively. Among the QTL detected at 5% genome-wise level, four QTL had Mendelian mode of inheritance on SSCs 5, 10, 12, and 13 for cooking loss, drip loss, crude lipid and crude protein, respectively; two QTL maternal inheritance for pH at 24-h and shear force on SSC11; three QTL paternal inheritance for CIE b and Hunter b on SSC9 and for cooking loss on SSC15; and one QTL partial expression for crude ash on SSC13, respectively. Most of the Mendelian QTL (9 of 13) had a dominant mode of gene action, suggesting potential utilization of heterosis for genetic improvement of meat quality within the cross population via marker-assisted selection.