• Herbal Formula Science
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• v.18 no.1
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• pp.95-103
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• 2010

Objectives : To develop and validate HPLC-PDA methods for simultaneous determination of seven constituents in Samsoeum(SSE). Methods : Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array(PDA) detection at 254 and 280 nm, were used for quantification of the seven marker components of SSE. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid. Results : Calibration curves were acquired with $r^2$>0.9997, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each compound was in the range of 100.07-112.65%, with an RSD less than 4.0%. The contents of seven compounds in SSE were 1.24-10.53 mg/g. Conclusions : The established method will be helpful to improve quality control of SSE.

• Genomics & Informatics
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• v.19 no.3
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• pp.30.1-30.8
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• 2021

Salmonella species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Salmonella species. We designed LAMP primers targeting the hilA gene as a universal marker of Salmonella species. A total of seven Salmonella species strains and 11 non-Salmonella pathogen strains from eight different genera were used in this study. All Salmonella strains showed positive amplification signals with the Salmonella LAMP assay; however, there was no non-specific amplification signal for the non-Salmonella strains. The detection limit was 100 femtograms (20 copies per reaction), which was ~1,000 times more sensitive than the detection limits of the conventional polymerase chain reaction (PCR) assay (100 pg). The reaction time for a positive amplification signal was less than 20 minutes, which was less than one-third the time taken while using conventional PCR. In conclusion, our Salmonella LAMP assay accurately detected Salmonella species with a higher degree of sensitivity and greater rapidity than the conventional PCR assay, and it may be suitable for point-of-care testing in the field.

• Journal of Information Technology Applications and Management
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• v.29 no.6
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• pp.23-42
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• 2022

This paper introduces two R&D policy instruments of public R&D and business R&D subsidy into the growth model of endogenous innovation simultaneously, and studies how they respectively affect economic growth, as well as how they interact with each other for the growth effects. Main results are as follow. The growth effects of each instrument are non-monotonous but various depending on the structure of economy. For example, the growth effects of public R&D become positive in the economy of more innovation-friendly structure such as larger marker size, more monopolistic market structure and more patient consumer, but the growth effects of business R&D subsidy become positive in the economy of less innovation-friendly structure such as smaller market size, more competitive market structure and more inpatient consumer. Meanwhile business R&D subsidy does not affect the growth effects of public R&D, but public R&D affects the growth effects of business R&D subsidy. Particularly, in the economy of less innovation-friendly structure public R&D becomes complimentary to business R&D subsidy.

• Lingua Humanitatis
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• v.7
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• pp.11-30
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• 2005

Historical origins of both personal names and place names in Korea are reviewed. It is shown that names of native origin have been largely replaced by those of Sino-Korean names. Some statistics are given on the basis of the 2000 census data in South Korea. A unique method of naming personal names which contain a generation marker called hangnyol is reviewed. This enables the person to figure out one's position and others in the family tree up to as many as ten generations without going consulting the book of genealogy. While this practice had a role to play in a vertically structured society where seniority is important, it is less practiced as the society is becoming more egalitarian, so that native names, not writable in Chinese characters, are on the rise. In this global age, a person is not just a member of his family or clan, s/he is also a member of the international community. The author proposes several things that should be considered in naming to fit the modern global age: euphony of names, ambiguity, possible bad connotations when Romanized, unintended homophones with comic meanings, etc.

• Proceedings of the IEEK Conference
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• 2008.06a
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• pp.951-952
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• 2008

This paper proposes a new method for image composition which estimates the rotation angle of human hand and uses the reserved image in real-time camera images. First, we capture a background image and extract a interesting region by background subtraction. Next, we estimate the skin region from the interesting region and calculate the rotation angle of estimated skin region using PCA(Principal Components Analysis). Finally, we composite the reserved image for the calculated rotation angle in camera images. The proposed method can be applied to control the 3D avatar for marker-less augmented reality.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.1723-1726
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• 2012

Objective: To summarize and evaluate various urinary markers for early detection, diagnosis and follow-up of human bladder cancer. Methods: A MEDLINE and PUBMED search of the latest literature on urinary markers for bladder cancer was performed. We reviewed these published reports and made a critical analysis. Results: Most urinary markers tend to be less specific than cytology, yielding more false-positive results, but demonstrating an advantage in terms of sensitivity, especially for detecting low grade, superficial tumors. Some tumor markers appear to be good candidates for early detection, diagnosis, and follow-up of human bladder cancer. Conclusion: A number of urinary markers are currently available that appear to be a applicable for clinical detection, diagnosis, and follow-up of bladder cancer. However, further studies are required to determine their accuracy and widespread applicability.

• The Journal of Korea Robotics Society
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• v.2 no.2
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• pp.143-151
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• 2007

This paper deals with a tangible interface system that introduces robot as remote avatar. It is focused on a new method which makes a robot imitate human arm motions captured from a remote space. Our method is functionally divided into two parts: capturing human motion and adapting it to robot. In the capturing part, we especially propose a modified potential function of metaballs for the real-time performance and high accuracy. In the adapting part, we suggest a geometric scaling method for solving the structural difference between a human and a robot. With our method, we have implemented a tangible interface and showed its speed and accuracy test.

• Journal of Sensor Science and Technology
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• v.22 no.2
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• pp.111-117
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• 2013

The autonomous driving method using magnetic sensors recognizes the position by measuring magnetic fields in autonomous robots or vehicles after installing magnetic markers in a moving path. The Position estimate method using magnetic sensors has an advantage of being affected less by variation of driving environment such as oil, water and dust due to the use of magnetic field. It also has the advantages that we can use the magnet as an indicator and there is no consideration for power and communication environment. In this paper, we propose an efficient sensor system for an autonomous driving vehicle supplemented for existing disadvantage. In order to efficiently eliminate geomagnetism, we analyze the components of the horizontal and vertical magnetic field. We propose an algorithm for position estimation and geomagnetic elimination to ease analysis, and also propose an initialization method for sensor applied in the vehicle. We measured and analyzed the developed system in various environments, and we verify the advantages of proposed methods.

• Herbal Formula Science
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• v.18 no.2
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• pp.251-257
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• 2010

Objectives : To develop and validate High-performance liquid chromatography-photodiode array methods for simultaneous determination of two constituents in Oryeong-san(ORS). Methods : Reverse-phase chromatography using a Gemini C18 column operating at $40^{\circ}C$, and photodiode array(PDA) detection at 280 nm, were used for quantification of the two marker components of ORS. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was $H_2O$ and solvent B was acetonitrile. Results : Calibration curves were acquired with correlation coefficient ($r^2$)>0.9999, and the relative standard deviation(RSD) values(%) for intra- and inter-day precision were not exceed 1.0%. The recovery rate of each compound was in the range of 93.01-104.16%, with an RSD less than 2.0%. The contents of two compounds in ORS were 1.10-3.72 mg/g. Conclusions : The established HPLC method will be helpful to improve quality control of ORS.

• Animal cells and systems
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• v.10 no.2
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• pp.65-71
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• 2006

Autosomal Dominant Polycystic Kidney Disease (ADPKD) is one of the most common inherited renal disorders in the world. Mutations in PKD1 located on chromosome 16p13.3 are responsible for 85% of all the ADPKD patients whereas mutations in PKD2 on chromosome 4q21-23 are responsible for the rest of the cases. Genetic heterogeneity and the problems of mutation detection in PKD1 suggest that linkage analysis is an important approach to study the genetics of ADPKD. To evaluate the availability of six (CA)n microsatellite markers for the linkage analysis of ADPKD in the Korean population, we examined the allele frequencies and heterozygosities of the markers. With the exception of KG8, five markers were highly informative, with PIC values over 0.5, but the PIC value of KG8 marker was less informative than other five markers because of the low number of alleles. Therefore, this study will be useful in linkage analysis for ADPKD families in the Korean population.