• Journal of the Optical Society of Korea
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• v.17 no.2
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• pp.188-199
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• 2013

This paper presents a novel algorithm for nighttime detection of the lane markers painted on a road at night. First of all, the proposed algorithm uses neighborhood average filtering, 8-directional Sobel operator and thresholding segmentation based on OTSU's to handle raw lane images taken from a digital CCD camera. Secondly, combining intensity map and gradient map, we analyze the distribution features of pixels on boundaries of lanes in the nighttime and construct 4 feature sets for these points, which are helpful to supply with sufficient data related to lane boundaries to detect lane markers much more robustly. Then, the searching method in multiple directions- horizontal, vertical and diagonal directions, is conducted to eliminate the noise points on lane boundaries. Adapted Hough transformation is utilized to obtain the feature parameters related to the lane edge. The proposed algorithm can not only significantly improve detection performance for the lane marker, but it requires less computational power. Finally, the algorithm is proved to be reliable and robust in lane detection in a nighttime scenario.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.71.2-71
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• 2003

Ralstonia solancearum infects many solanaceous plants, however race 3 infects only potato and tomato weakly. To identify genes responsible for race specificity of R. solanacearum, we mobilized genomic library of LSD2029 (race 3) into LSD341 (race 1) and inoculated 1,000 transconjugants into hot pepper. One transconjugant that did not induce wilt symptom in hot pepper was isolated. We found that a cosmid clone, pRSl, conferred avirulence to LSD341. By deletion and mutational analyses of pRSl, we found the 0.9-kb PstI/Hindlll fragment carries avirulence functions. We sequenced the fragment and identified one possible open reading frame, a rsal gene, possibly encoding 110 amino acids. The rsal was preceded with a plant-inducible promoter (PIP) box, indicating that the gene might be regulated by HrpB. Interestingly, the promoter region of the rsal homolog in the strain GM11000 (race 1) did not have the PIP box. Rsal did not show any significant homologies with proteins in the database, indicating th e protein is different from the previously reported avirulence proteins. When we mutated the rsal gene by marker-exchange in LSD2029, the mutant was less virulent in potato.

• Language and Information
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• v.18 no.1
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• pp.1-32
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• 2014

This paper provides a corpus study on subjunctives in Korean in a way of comparative semantics. The whole arguments of this paper are bolstered by distributional evidence taken from naturally occurring bitexts (i.e. a bilingual corpus), in which one sentence in a language is aligned with one translation in the other language. Since previous studies regard past tense morphology as the main component to express irrealis and uncertainty, this paper accordingly checks out whether the past tense morpheme (e/a)ss in Korean is also responsible for conveying the meaning of subjunctives. My finding is that the past tense morpheme (e/a)ss is a sufficient condition for forming subjunctives in Korean. The current corpus study verifies that the past tense morpheme is not obligatorily used in present conditional counterfactuals in Korean, unlike English. Yet, if (e/a)ss is used and the antecedent denotes a present situation, the conditional sentence can only be interpreted as conveying counterfactuality. On the other hand, wish constructions in Korean, irrespective of the semantic tense, often contain the past tense morpheme. Hence, this work substantiates Iatridou (2000)'s theory of 'fake past tense' is applicable to Korean subjunctives. The present corpus study, additionally, reveals that a conditional marker telamyen is a component of expressing past counterfactuals in Korean.

• Journal of Microbiology and Biotechnology
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• v.1 no.3
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• pp.151-156
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• 1991

In order to construct a yeast strain having high ethanol tolerance together with good lactose fermentation ability, the protoplast fusion using Saccharomyces cerevisiae STV 89 and Kluyveromyces fragilis CBS 397 was carried out. Auxotrophic mutants of K. fragilis were obtained as a selection marker by treatment of ethylmethane sulfonate. The best mutant for protoplast fusion was selected based on the capabilities of ${\beta}-galactosidase$ production and lactose fermentation. The protoplast fusion using polyethylene glycol and calcium chloride solution led to the fusion frequence of $3{\times}10^{-6}$ and a number of fusants were obtained. Among these fusants, a fusant F-3-19 showed the best results in terms of ethanol tolerance, ${\beta}-galactosidase$ activity and lactose fermentation. The performance of lactose fermentation and ethanol tolerance by this fusant were better than those of K. fragilis. Study on the ethanol tolerance having relation to fatty acid composition and intracellular ethanol concentration revealed that the fusant F-3-19 had a higher unsaturated fatty acids content and accumulated less amount of intracellular ethanol compared with a parent of K. fragilis.

• Microbiology and Biotechnology Letters
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• v.23 no.5
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• pp.536-543
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• 1995

Multicopy integration vector is a very useful vector system in that they can be integrated into chromosomal DNA in several copies and stably maintained under non-selective conditions. To develop a multicopy integration vector system in the yeast Yarrowia lipolytica, P-type ribosomal DNA was cloned from Y lipolytica. A HindIII-BglII fragment of the cloned rDNA and a promoterless URA3 gene were inserted into pGEM1, generating multicopy integration vectors, pMIYL-1 and pMIYL-2. The rDNA fragment is for targeted homologous recombination between the vector and the chromosomal DNA of Y. lipolytica, and the promoterless URA3 gene is a defective selection marker for inducing multicopy integration. pMIYL-1 and pMIYL-2 have an unique restriction enzyme site, KpnI, and two unique restriction enzyme sites, KpnI and EcoRI, repectively, which can be used for targeting of the vectors into the rDNA of Y. lipolytica chromosomal DNA. After transformation of the vectors into Y. lipolytica, copy number and stability were analyzed by Southern hybridization. The vectors were found to be present in less than 5 copies per cell and were stably maintained during growth in non-selective media.

• Journal of Advanced Navigation Technology
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• v.13 no.1
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• pp.126-133
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• 2009

In this paper, we propose an object recognition technique for implementing marker less augmented reality. Scale Invariant Feature Transform (SIFT) is used for finding the local features from object images. These features are invariant to scale, rotation, translation, and partially invariant to illumination changes. Extracted Features are distinct and have matched with different image features in the scene. If the trained image is properly matched, then it is expected to find object in scene. In this paper, an object is found from a scene by matching the template images that can be generated from the first frame of the scene. Experimental results of object recognition for 4 kinds of objects showed that the proposed technique has a good performance.

• Journal of Korea Game Society
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• v.11 no.3
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• pp.63-72
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• 2011

In this paper, we propose a novel database creation and retrieval method of feature descriptors to support real-time marker-less tracking in the augmented reality environments. Each feature descriptor is encoded by integer and multi-level database is created in order to retrieve a feature descriptor efficiently. The retrieval of a feature descriptor is performed as follows: Firstly, candidate feature descriptors are searched by traversing the multi-level database. Secondly, the euclidean distance between input feature descriptor and each candidate one is compared. The shortest one is retrieved. The proposed method is 16 ms faster than previous KD-Tree method for each feature descriptor.

• Korean Journal of Pharmacognosy
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• v.40 no.3
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• pp.224-228
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• 2009

For the quality control of traditional herbal medicine, Galgeun tang, simultaneous determination of glycyrrhizin, paeoniflorin, puerarin, 6-gingerol was established by using a high performance liquid chromatographic (HPLC) method with diode array detector. To separate five four constituents, DIONEX $C_{18}$ column ($5{\mu}m$, $120{\AA}$, $4.6\;mm{\times}150\;mm$) was used with gradient elution system of water and methanol. Validation of the chromatography method was evaluated by linearity, recovery, and precision test. Calibration curve of standard components showed excellent linearity ($R^2$>0.9906). Limits of detection (LOD) and limits of quantification (LOQ) varied from 0.15 to $0.52{\mu}g$/ml and 0.27 to $0.80{\mu}mg$/ml, respectively. The relative standard deviations (RSDs) of data of the intra-day and inter-day experiments were less than 2.88% and 1.21%, respectively. The results of recovery test were ranged from 96.71 to 106.29% with RSD values 0.01-0.80%.

• Natural Product Sciences
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• v.21 no.3
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• pp.192-195
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• 2015

An antibacterial benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, isolated from Ficus foveolata stems was used as a standard marker for establishment of quantitative HPLC analysis for the stem extracts of F. foveolata. The method utilized a TSK-gel ODS-80Ts column (5 μm, 4.6 × 250 mm) with the mixture of methanol and 5% acetic acid in water (24:76, v/v) as the mobile phase at a flow rate of 1 mL/min, and quantitative detection at 289 nm. The parameters i.e. linearity, intraday and interday precision, accuracy, specificity and sensitivity of the method were evaluated for method validation. The recoveries of the method were 99.5 - 103.6% and good linearity (R² ≥ 0.9999) was obtained. A high degree of specificity, sensitivity as well as repeatability and reproducibility (RSD less than 2 and 5%, respectively) were also achieved. Chloroform was served as the most suitable solvent for extraction of 2,6-dimethoxy-1,4-benzoquinone. The optimised sample preparation and HPLC method can be practically used in the routine quality control process of F. foveolata stem extracts.

• The Korean Journal of Cytopathology
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• v.17 no.1
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• pp.18-26
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• 2006

Transitional cell carcinoma of the urinary bladder is common in the genitourinary tract. The gold standard for the diagnosis of bladder cancer has been cystoscopy, along with urine cytology. Cystoscopy is an invasive and relatively expensive technique. By comparison, urine cytology is easy to perform and specific for a diagnosis of bladder cancer, although less sensitive, especially in low-grade tumors. For this reason, there has been a need for superior noninvasive technology to increase our confidence in being able to detect bladder cancer. There are many reports of the various urinary tests that are available to facilitate the diagnosis. In this article, I reviewed the literature on urinary markers and tests that may be clinically useful, including fluorescence in situ hybridization, uCyt+/Immunocyte, the $BTA^{(R)}$ test, the NMP 22TM, the $FDP^{(R)}$ test, the telomerase activity test, the HA and HAse tests, and flow cytometry. Most of these tests have a higher sensitivity and specificity than cytology. However, urine cytology has the highest specificity, especially in individuals with a high-grade tumor. We conclude that no urinary markers or tests can replace the role of cystoscopy along with cytology in the diagnosis of transitional cell carcinoma of the bladder. However, some markers could be used adjunctively to increase the diagnostic accuracy during screening or during the postoperative follow-up examination of patients with bladder cancer.