• 복식문화연구
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• 제18권1호
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• pp.13-28
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• 2010

This study is focused on the elucidation of efficient and correct marking methods by comparing and analyzing marker efficiency depending on the marking job condition such as interval of checking-pattern and marker orientation using woman's Jacket and Slacks. Research Method intended to compare the marking efficiency of Jacket and Slacks, check-less and check- patterned materials with the intervals of $1.5{\times}1.5cm$ and $5{\times}5cm$ were selected. First, in the comparison of marking efficiency depending on check-pattern interval, regardless of the direction of marker position, single-item makers and set-item makers, the efficiency of check-less materials was higher than those of check-patterned materials and increasing the intervals decreased marking efficiency and vice versa. Marking efficiency of Slacks was less influenced than marking efficiency of Jacket by check-pattern interval. Second, in the comparison of marking efficiency depending on the direction of marker position, regardless of check-pattern interval and number of maker pieces, the efficiency of nap-up-and-down position was higher than those of nap-one-way position. Third, the marking efficiency of single-item was more effective than one of set-item in all working condition except nap-one-way position in checkless materials.

• 정보처리학회논문지A
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• 제17A권4호
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• pp.189-196
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• 2010

본 논문에서는 모서리점, 경계선 및 영역, 적응적 임계값 등과 같은 복합특징을 이용하여 증강현실 시스템에서 마커의 차단현상이 발생되거나 어두운 환경에서도 사용 가능하면서 정합 성능을 개선한 마커검출 알고리즘을 제안한다. 기존의 ARToolkit에서는 마커의 일부분이 사용자에 의해 가려지거나 주위 조명 변화에 의해 입력영상의 밝기 변화가 크게 될 경우, 마커를 추출할 수 없는 반면 제안한 마커추적 알고리즘에서는 마커영역 추출시 적응적 임계값 기법을 사용하여 조명의 변화에 둔감하게 반응하여 정확한 마커영역만을 분리 추출할 수 있다. 그리고 모서리 여부를 판단하고 모서리점이 가려진 경우, 추출된 직선의 교점으로부터 모서리점을 추출하므로 차단에 의해 마커가 가려졌을 때에도 정확한 마커 영역을 추출할 수 있다. 또한, 등록된 마커와의 정합시, 와핑에서 발생되는 마커의 크기 및 중심위치 변화를 보정하는 기법을 추가하여 정합 성능을 개선 시켰다. 실험 결과 제안한 알고리즘은 주위 조명 변화와 차단 현상에 강인하게 마커를 검출하였으며, 유사한 마커 태그를 구분 할 수 있는 정합 유사도가 종전보다 30% 증가한 것을 확인 할 수 있었다.

• 한국안전학회지
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• 제21권6호
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• pp.82-87
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• 2006

The existing raised pavement marker, however, have some limitations. Most of them are square-shaped which makes only forward and backward retro-reflection, their orientation can be easily changed by impact of vehicle, and the surface reflector can be readily contaminated leading poor functioning. In addition, maintenance for broken raised pavement marker is not easy. The purpose of the study is to design and manufacture a new and improved type of raised pavement marker. The new raised pavement marker has round-shaped upper structure. Its body was separated from the holder and the reflector was surrounded by acrylic cover. We tested performances of the constituent parts, optimum load after installation, and reflection efficiency. The new raised pavement marker will be an initiation of studies on the improvement of the shape and performance of raised pavement markers.

• 한국수산과학회지
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• 제40권3호
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• pp.128-132
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• 2007

We identified a microsatellite marker, Poli121TUF, which appears to be significantly linked (P<0.001) with a lymphocystis disease virus (LCDV)-resistance gene in the olive flounder, Paralichthys olivaceus. The olive flounder is an economically important food fish, that is widely cultured in Korea, Japan, and China. Lymphocystis disease has spread in these countries and has seriously reduced the economic value of the fish. LCDV causes lymphocystis cells (LC) to form on the body surface, fins, gills, mouth, and intestine. Fish with LC lose commercial value due to their deformed appearance. The identified micro satellite marker can be used as a candidate locus for marker-assisted selection (MAS) in order to enhance the efficiency of selection for LCDV resistance in the olive flounder.

• 한국육종학회지
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• 제40권1호
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• pp.48-53
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• 2008

본 연구에서는 벼멸구 저항성 개통 육성과정 중에 수행한 PS와 MAS system간의 선발 효율을 정확성, 소요시간, 소요비용 등의 요인으로 비교 분석함으로써 MAS system의 선발 효율을 제시하고, 이를 이용하여 효과적인 MAS system을 구축하고 실용화하기 위한 방안을 제시하였다. DNA marker를 이용하여 벼멸구 저항성 개체를 선발하고 MAS 체제를 확립하기 위하여 '삼강/낙동' 조합의 $F_2$집단, RIL($F_6$), DH집단, 여교잡집단($BC_6F_5$)을 대상으로 PS와 marker 검정을 병행하여 두 검정 방법 간에 각 잡종집단 및 세대별 저항성과 감수성 계통(개체)의 비율을 비교한 바 95%이상 일치하였다. MAS 체제와 PS간의 선발효율을 비교한 바 MAS는 PS에 비해 약 32%의 비용이 절감되었고, 검정에 소요되는 기간도 기존의 PS에서는 30일인데 비해 MAS에서는 7일로 크게 단축되었다. 이 결과를 바탕으로 MAS에 의한 벼멸구 저항성 계통 선발 체계를 확립 할 수 있었다.

• 생명과학회지
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• 제30권3호
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• pp.239-249
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• 2020

감 꽃은 과실을 맺는 암꽃과 꽃가루를 내는 수꽃, 암술과 수술을 모두 가진 양성화가 있다. 관행 교배육종법으로 부본용 수꽃 신품종을 육성한다면 최종 선발까지 15년 정도의 장기간과 넓은 시험포장이 요구된다. 유묘기에 수꽃 착생 개체를 조기에 선발하여 육종효율을 증진하고자 꽃의 성표현을 알고 있는 주요 품종 88개체를 대상으로 Akagi 등(2014)이 보고한 수꽃 선발 마커들의 적용 효과를 검증하였다. OGI locus marker와 DISx-AF4S marker 적용 결과 각각 83품종, 72품종에서 꽃의 성표현과 분석 결과가 일치하였다. 육성품종인 '미감조생'을 모본으로 한 교배조합 등으로부터 나온 실생 2,509개체에 OGI locus marker 수꽃 선발 마커를 적용하여 890개체를 선발하였고, 분석 완료한 교배실생 중 아직 착과가 되지 않았거나 고사한 개체를 제외한 1,186개체를 대상으로 꽃의 성표현과 마커 분석 결과를 비교하였을 때 401개체가 불일치한 것을 확인하였다(33.8%). 다양한 교배조합으로부터 나온 실생 889개체에 DISx-AF4S marker를 적용하여 분석한 결과 636개체를 선발하였다. 분석 완료한 교배실생 중 아직 착과가 되지 않았거나 고사한 개체를 제외한 379개체를 대상으로 꽃의 성 표현과 마커 분석 결과를 비교하였고 247개체가 불일치한 것을 확인하였다(65.2%). 이상의 결과들을 종합하여 볼 때, 검토한 DISx-AF4S, OGI locus marker는 육종현장에서 활용하기에 유용하지 않을 것으로 판단된다.

• Journal of the Korean Wood Science and Technology
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• 제48권5호
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• pp.745-754
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• 2020

The dye marker Sudan Red 7B was tested as an effective and appropriate marking method for searching the territory of the termite, Reticulitermes speratus, in Korea. The sensitivity of termites to the dye marker was evaluated by measuring the feed consumption amount, survival rate, retention time, and transfer efficiency of R. speratus according to the concentration and feeding period of Sudan Red 7B. As the concentration conditions of dye marker increase, Sudan Red 7B remarkably reduced the feed consumption of the termites. The survival rate was not significantly different from that of the control group (more than 90%), except when feed containing 1% or 2% concentrations of the dye marker was given for five days. The duration of the marking effect persisted for seven days, even at a concentration of 2% dye marker in the feed. No dye transfer by trophallaxis occurred between termites marked with Sudan Red 7B and unmarked termites.

• 원예과학기술지
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• 제33권4호
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• pp.550-558
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• 2015

To analyze the linkage relationships among molecular markers recently reported to be linked to onion (Allium cepa L.) Ms, a restorer-of-fertility locus, in onion (Allium cepa L.), three single nucleotide polymorphism markers were converted into cleaved amplified polymorphic sequence (CAPS) markers based on onion transcriptome sequences and the rice genome database. Analysis of the recombinants selected from 4,273 segregating plants using CAPS and other linked markers demonstrated the jnurf13 and jnurf610 markers to perfectly co-segregate with the Ms locus. In contrast to jnurf13, the jnurf610 marker was not in perfect linkage disequilibrium with the Ms locus in diverse breeding lines. Thus, the jnurf13 marker and the marker for identification of cytoplasm types were utilized to enhance the efficiency of onion breeding through four applications. First, 89 maintainer lines containing the normal cytoplasm and homozygous recessive Ms genotypes were successfully identified from 100 breeding lines. Second, these two molecular markers were used to analyze the main sources of male-fertile contaminants frequently found in the male-sterile parental lines during F1 hybrid seed production. The majority of the contaminants contained heterozygous Ms genotypes, indicating that pollen grains harboring the dominant Ms genotype may have been introduced during propagation of the maintainer lines. Therefore, the genetic purity of the two maintainer lines was analyzed in the third application, and the results showed that both maintainer lines contained 13-21% off-types. Finally, the two markers were used to increase the seed yield potentials of two open-pollinated varieties containing sterile cytoplasms by removing the plants harboring homozygous recessive and heterozygous Ms genotypes.

• 미생물학회지
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• 제20권1호
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• pp.1-10
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• 1982

From Aspergillus nidulans, six suppressor mutants, MSI-MS6, were isolated, and their characteristics were analysed. These were the suppressor mutants for acriflavin resistant and nicotinamide auxotrophic mutant phenotypes. MS1, MS2, MS5 and MS6, were linked to the chromosome IV, I, II respectively, and MS2 was linked to one of the rest chromosomes, MS3 and MS6 mutants had both suppressors for acriflavin resistant marker and for nicotinamide auxotrophic marker. In order to know the stability and efficiency of the suppressors, their reversion frequencies, that is, frequencies of losing the suppressibility, were analysed. Only MS3 and MS5 were reversed with high frequency. The four mutants didn't lose their suppressibilities, and this meant that the suppressors of these four were very stable and highly effcient. The suppressor specificities of these mutants were tested for other mutant's phenotype marker. One of the six suppressors, MS1, had the suppressor specificity for acriflavin resistant marker of 163 strain.

• Reproductive and Developmental Biology
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• 제38권2호
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• pp.71-77
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• 2014

The specific genetic modification in porcine somatic cells by gene targeting has been very difficult because of low efficiency of homologous recombination. To improve gene targeting, we designed three kinds of knock-out vectors with ${\alpha}1,3$-galactosyltransferase gene (${\alpha}1,3$-GT gene), DT-A/pGT5'/neo/pGT3', DT-A/NLS/pGT5'/neo/pGT3' and pGT5'/neo/ pGT3'/NLS. The knock-out vectors consisted of a 4.8-kb fragment as the 5' recombination arm (pGT5') and a 1.9-kb fragment as the 3' recombination arm (pGT3'). We used the neomycin resistance gene (neo) as a positive selectable marker and the diphtheria toxin A (DT-A) gene as a negative selectable marker. These vectors have a neo gene insertion in exon 9 for inactivation of ${\alpha}1,3$-GT locus. DT-A/pGT5'/neo/pGT3' vector contain only positive-negative selection marker with conventional targeting vector. DT-A/NLS/pGT5'/neo/pGT3' vector contain positive-negative selection marker and NLS sequences in upstream of 5' recombination arm which enhances nuclear transport of foreign DNA into bovine somatic cells. pGT5'/neo/pGT3'/NLS vector contain only positive selection marker and NLS sequence in downstream of 3' recombination arm, not contain negative selectable marker. For transfection, linearzed vectors were introduced into porcine ear fibroblasts by electroporation. After 48 hours, the transfected cells were selected with $300{\mu}g/ml$ G418 during 12 day. The G418-resistant colonies were picked, of which 5 colonies were positive for ${\alpha}1,3$-GT gene disruption in 3' PCR and southern blot screening. Three knock-out somatic cells were obtained from DT-A/NLS/ pGT5'/neo/pGT3' knock-out vector. Thus, these data indicate that gene targeting vector using nuclear localization signal and negative selection marker improve targeting efficiency in porcine somatic cells.