• 한국수산과학회지
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• 제50권6호
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• pp.669-674
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• 2017

Paralytic shellfish toxins (PSTs) are produced by marine dinoflagellate phytoplankton Alexandrium spp. and Gymnodinium spp. These toxins accumulate in filter feeding organisms such as bivalves and the ingestion of contaminated shellfish can cause illness in humans. The mouse bioassay (MBA) has been the preferred PST testing method worldwide for more than 50 years. However, this assay has several disadvantages, such as detection limits, non-toxic-profiles, and the ethical issues of using animals. The aim of this study was to establish an alternative to the MBA method for testing for PSTs. We optimized the analysis conditions of a post-column oxidation-high performance liquid chromatography (PCOX-HPLC) method and the Scotia Rapid Test Kit, and then compared the accuracy of these methods to the MBA method. The results demonstrated a strong correlation between the PCOX-HPLC method and the MBA, although the PCOX-HPLC method required expensive equipment and standard material, and was time consuming. The Scotia Rapid Test Kit promises to be a useful tool, as it provided rapid and qualitative results, although the method sometimes gave a false positive result that could not be explained by toxin profiles.

• 한국수산과학회지
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• 제29권6호
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• pp.893-899
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• 1996

The studies on the detoxification of paralytic shellfish poison (PSP)-infested blue mussels, Mytilus edulis and oyster, Crassostrea gigas were performed for using of available processing resource. Toxic blue mussel and oysters from Nampo in Masan Bay, Hachong in Koje Bay and Woepori in Koje were used for experimental samples. The toxicity of low toxic blue mussel $(A,\;84{\mu}g/100g;\;B,\;166{\mu}g/100g;\;C,\;295{\mu}g/l00g;\;D,\;557{\mu}g/100g)$ and oyster $(740{\mu}g/100g)$ were reduced below the regulation limit of PSP $(80{\mu}g/100g)$ or undetected level by mouse bioassay after boiling at $98^{\circ}C$ for 10 min and retorting at $115^{\circ}C$ for 70 min, while the toxicity of high toxic blue mussel $(E,\;8,760{\mu}g/100g)$ remained beyond the regulation limit after boiling and retorting at same condition. These results suggested that the regulation limit of PSP could be level up from $(80{\mu}g/100g)$ to about $160{\mu}g/100g$.

• 한국수산과학회지
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• 제33권6호
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• pp.550-553
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• 2000

해수에서 분리한 마비성패류독 분해 균주 Enterobactersp. CW-6 균주의 마비성 패류독소 최적 분해 온도 및 시험 균주의 독소 분해과정 중의 독소 함량 및 구성성분을 조사한 결과는 다음과 같다. 시험 균주는 배양 적온으로 확인된 $30, 35^{\circ}C$에서 배양 5일만에 각각 최초 첨가 독력의 $56.8, 44.1{\%}$, 12일 후에는 $93.0, 79.5{\%}$를 분해하였다. 그러나, 배양 적온에서 벗어날수록 균주의 독소 분해 활성은 감소하여 $20^{\circ}C$에서는 배양 12일 후에도 최초 독력의 $69.0{\%}$가 잔존하였다. 시험 균주 Enterobacter sp. CW-6는 $30^{\circ}C$에서의 조독소를 이용한 분해 활성 측정에서 초기 농도 38.2nmole/g의 마비성패류독을 배양 8일 및 12일째 각각 $88.4, 92.7{\%}$ 분해하였다. 정제 독소를 이용한 분해 시험에서, 조독소 분해과정 중에 일시적으로 증가하는 STX group은 GTX2, 3에서 전환된 것으로 확인되었다. 시험 균주 Enterobactersp. CW-6는 정제 독소에 대해서도 강한 분해 활성을 나타내었으며, 최초 농도 47 nmole/g 의 GTX1과 37 nmole/g GTX4를 분해 과정 12일 후에 각각 $100, 90.8{\%}$ 분해하였으며, GTX2, 3 혼합물에 대하여는 최초농도 25.6 nmole/g의 독소를 12일 후에 $66.4{\%}$까지 분해하였다.