• Microbiology and Biotechnology Letters
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• v.43 no.4
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• pp.314-321
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• 2015

Agar-hydrolyzing bacteria were isolated from the coastal sea water of Jeju Island. One isolate, designated as S4, was selected for further study. The S4 cells were Gram-negative and rod-shaped with smooth beige surfaces and single polar flagellum. Cells were grown at $15-42^{\circ}C$, 0.5-5% (w/v) NaCl, between pH 6.0 and 9.0, and in media containing 0.5-5% (w/v) NaCl. The G+C content was 49.93 mol%. The major fatty acids (>15%) were $C_{18:1}{\omega}7c$, $C_{16:0}$ and Summed feature 3 (comprising $C_{16:1}{\omega}7c/iso-C_{15:0}$ 2-OH). Based on 16S rRNA sequencing and biochemical and chemotaxonomic characteristics, the strain was designated as Vibrio sp. S4. In liquid culture supplemented with 0.1% agar the cell density and agarase activity reached a maximum level in 72 h, while agarase activity in the culture without agar was negligible, implying agarose expression is induced by agar. The optimum pH and temperature for the extracellular crude agarase of S4 were 7.0 and $45^{\circ}C$, respectively. However, it also exhibited 98.6% and 87.6% at $40^{\circ}C$ and $50^{\circ}C$, respectively, of the maximum activity seen at $45^{\circ}C$. The crude agarase hydrolyzed agarose into (neo)agarotetraose and (neo)agarohexaose.

• Microbiology and Biotechnology Letters
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• v.40 no.3
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• pp.243-249
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• 2012

This study was conducted to screen an alginate-degrading microorganism and to investigate the characteristics of the alginate-degrading activity of its crude enzyme. A marine bacterium which produces extracellular alginate-degrading enzymes was isolated from the brown alga Sargassum thunbergii. 16S rRNA sequence analysis and physiological profiling resulted in the bacterium's identification as a Vibrio crassostreae strain, named Vibrio crassostreae PKA 1002. Its optimal culture conditions for growth were pH 9, 2% NaCl, $30^{\circ}C$ and a 24 hr incubation time. The optimal conditions for the alginate degrading ability of the crude enzyme produced by V. crassostreae PKA 1002 were pH 9, $30^{\circ}C$, a 48 hr incubation time and 8% alginic acid. The alginate degrading crude enzyme produced 3.035 g of reducing sugar per liter in 4% (w/v) alginate over 1 hr.

• Microbiology and Biotechnology Letters
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• v.38 no.2
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• pp.144-150
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• 2010

This study was aimed to screen bacteria of high alginate-degrading activity, to select the nitrogen source and concentration of NaCl and sodium alginate for the production of alginate-degrading enzyme, and to determine reaction conditions of enzyme. A novel alginate-degrading bacterium was isolated from abalone (Haliotis discus hannai) and named Methylobacterium sp. HJM27 by 16S rDNA sequence analysis. The optimum culture conditions for the production of alginate-degrading enzyme were 1.0% sodium alginate, 0.5% peptone, 0.3% yeast extract, 1.5% NaCl, $25^{\circ}C$ and 48 hours incubation time. The raw enzyme showed the highest activity at $25^{\circ}C$ and pH 9, and produced 1.217 g - reducing sugar per liter in 0.8% (w/v) sodium alginate for 30 minutes. Methylobacterium sp. HJM27 and its alginate-degrading enzyme would be useful for the production of bioenergy and biofunctional oligosaccharides from seaweed.

• Korean Journal of Environmental Agriculture
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• v.30 no.4
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• pp.424-431
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• 2011

BACKGROUND: Tributyltin chloride is among the most toxic compounds known for aquatic ecosystems. Microorganisms are responsible for removal of TBTCl. Nevertheless, only a limited number of marine bacteria were investigated for biodegradation of TBTCl in Korea. METHODS AND RESULTS: The number of TBTCl resistant bacteria ranged from $2.5{\times}10^3$ to $3.8{\times}10^3$ cfu/mL in the seawater, and ranged from $3.2{\times}10^5$ to $9.1{\times}10^5$ cfu/g in the surface sediment, respectively. The morphological, physiological, and biochemical characteristics of TBTCl resistant bacteria were investigated by API 20NE and other tests. The most abundant species of TBTCl resistant bacteria were Vibrio spp. (19.2%), Bacillus spp. (16.2%), Aeromonas spp. (15.2%), and Pseudomonas spp. (13.1%), etc. Eleven TBTCl resistant isolates also had a resistance to heavy metals (Cd, Cu, Hg, and Zn). Among them, isolate T7 showing the strong TBTCl-resistance was selected. This isolate was identified as the genus Pantoea by 16S rRNA gene sequencing and designated as Pantoea sp. T7. In addition, this bacterium was cultivated up to the growth of 50.7% after 60 hrs at TBTCl concentration of $500{\mu}M$. TBTCl-degrading activity of Pantoea sp. T7 was measured by GC-FPD analysis. As a result of biological TBTCl-degradation at TBTCl concentration of $100{\mu}M$, TBTCl-removal efficiency of Pantoeasp. T7 was 62.7% after 40 hrs. CONCLUSION(S): These results suggest that Pantoea sp. T7 is potentially useful for the bioremediation of TBT contamination.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.5
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• pp.547-556
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• 2016

Myometrial relaxation of mouse via expression of two-pore domain acid sensitive (TASK) channels was studied. In our previous report, we suggested that two-pore domain acid-sensing $K^+$ channels (TASK-2) might be one of the candidates for the regulation of uterine circular smooth muscles in mice. In this study, we tried to show the mechanisms of relaxation via TASK-2 channels in marine myometrium. Isometric contraction measurements and patch clamp technique were used to verify TASK conductance in murine myometrium. Western blot and immunehistochemical study under confocal microscopy were used to investigate molecular identity of TASK channel. In this study, we showed that TEA and 4-AP insensitive non-inactivating outward $K^+$ current (NIOK) may be responsible for the quiescence of murine pregnant longitudinal myometrium. The characteristics of NIOK coincided with two-pore domain acid-sensing $K^+$ channels (TASK-2). NIOK in the presence of $K^+$ channel blockers was inhibited further by TASK inhibitors such as quinidine, bupivacaine, lidocaine, and extracellular acidosis. Furthermore, oxytocin and estrogen inhibited NIOK in pregnant myometrium. When compared to non-pregnant myometrium, pregnant myometrium showed stronger inhibition of NIOK by quinidine and increased immunohistochemical expression of TASK-2. Finally, TASK-2 inhibitors induced strong myometrial contraction even in the presence of L-methionine, a known inhibitor of stretch-activated channels in the longitudinal myometrium of mouse. Activation of TASK-2 channels seems to play an essential role for relaxing uterus during pregnancy and it might be one of the alternatives for preventing preterm delivery.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.2
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• pp.59-69
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• 2003

As part of study on the microbial food web in the southern Jeju Island of Korea, Planktonic tintinnids and aloricate oligotrichs were investigated from July 1998 to June 2000 to understand seasonal variation and water mass indication with environmental factors. 39 species of tintinnids were identified representing 1 order, 11 families, 20 genera. Tintinnid abundance ranged from 100 to 5,400 cells.1$^{-1}$ (mean 314 cells.1$^{-1}$ ), and oceanic species were mainly dominant in fall and winter season, while neritic species were a little pre- dominant in spring and summer season. 15 species of aloricate oligotrichs were identified representing 1 order, 5 families, 7 genera, and many belonged to genus Strombidium which was most abundant and most frequently occurred in many months. Abundance of aloricate oligotrichs ranged from 140 to 21,000 cells.1$^{-1}$ (mean 2,356 cells.1$^{-1}$ ). Species diversity and standing crops of tintinnids were quite different according to seasons, but few seasonal variations were detected in aloricate oligotrichs. In terms of water mass indication tintinnids represented obvious characteristics which were affected by marine environmental factors, but alor- icate oligotrichs had no such apparent indication as tintinnids. Ciliates were more abundant and more diverse in inshore than in offshore station. Total carbon biomass of ciliates ranged from 0.01 to 136.06 $\mu\textrm{g}$C.1$^{-1}$ (mean 5.01 $\mu\textrm{g}$C.1$^{-1}$ ). The carbon biomass, however, did not coincided with seasonal variations of abun- dance Vertical profiles of mean abundance of both tintinnids and aloricate oligotrichs were similar, and had same trend as those of mean chlorophyll o concentration. It suggests that phytoplankton and ciliates reflected the prey-predator relationship in the study area.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.43 no.1
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• pp.49-61
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• 2007

Otter boards in the trawl are the one of essential equipments for the net mouth to be spread to the horizontal direction. Its performance should be considered in the light of the spreading force to the drag and the stability of towing in the water. Up to the present, studies of the otter boards have focused mainly on the drag and lift force, but not on the stability of otter boards movement in 3 dimensional space. In this study, the otter board is regarded as a rigid body, which has six degrees of freedom motion in three dimensional coordinate system. The forces acting on the otter boards are the underwater weight, the resistance of drag and spread forces and the tension on the warps and otter pendants. The equations of forces were derived and substituted into the governing equations of 6 degrees of freedom motion, then the second order of differential equations to the otter boards were established. For the stable numerical integration of this system, Backward Euler one of implicit methods was used. From the results of the numerical calculation, graphic simulation was carried out. The simulations were conducted for 3 types of otter boards having same area with different aspect ratio(${\lambda}=0.5,\;1.0,\;1.5$). The tested gear was mid-water trawl and the towing speed was 4k't. The length of warp was 350m and all conditions were same to each otter board. The results of this study are like this; First, the otter boards of ${\lambda}=1.0$ showed the longest spread distance, and the ${\lambda}=0.5$ showed the shorted spread distance. Second, the otter boards of ${\lambda}=1.0$ and 1.5 showed the upright at the towing speed of 4k't, but the one of ${\lambda}=0.5$ heeled outside. Third, the yawing angles of three otter boards were similar after 100 seconds with the small oscillation. Fourth, it was revealed that the net height and width are affected by the characteristics of otter boards such as the lift coefficient.

• Resources Recycling
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• v.29 no.5
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• pp.15-27
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• 2020

Microplastics are recognized as critical and serious environmental problem in worldwide. Plastics are inexpensive, lightweight, excellent in processability, and various in material-specific properties. Along industrial development, the production and disposal amount of plastics are also rapidly increasing. In particular, abundant plastic wastes are eventually disposed into marine environment with harmful impacts on the ecosystem. Therefore, lots of relevant studies were recently progressed in various fields. However, many studies are being just conducted due to its difficulty in applying a general treatment method for those small particle sizes and their various characteristics. In the meantime, lots of researches are being conducted on applying methods using physical properties such as specific gravity, magnetic, and electrostatic separation, which are beneficiation processes of minerals. However, since it is still in the laboratory stage, the development of larger scale separation technology for efficient treatment is urgent.

• Journal of the Korean GEO-environmental Society
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• v.17 no.5
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• pp.37-46
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• 2016

In order to determine the variability of environmental characteristics of lightweight air-foamed soil using marine clay according to freezing-thawing and soaking conditions, unconfined compressive strength of the lightweight air-foamed soil samples made by changing the amount of cement under curing conditions of outdoor low temperature, underground or indoor wetting were observed. Compressive strength was not increased under freezing-thawing (temperature range of $-9.1^{\circ}C{\sim}17.2^{\circ}C$) regardless of the amount of cement but the more cement using, it was increased rapidly by underground curing conditions within 30 cm beneath ground level. Therefore, it is necessary to install insulation layer cutting off exterior cold air after construction of lightweight air-foamed soil in condition of freezing-thawing. Bulk density was increased too small under the long-time soaking condition, it tended to decrease rapidly when samples were dried up and had below 6% of water contents. But variability of compressive strength and bulk density was very small for preventing drying and keeping its wet state. The lightweight air-foamed soil that installed beneath ground water level or covered by soil can be evaluated as a long-term reliable construction material.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.10
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• pp.1930-1936
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• 2007

Electronic Navigational Charts(ENCs) are official nautical charts which are equivalent to paper charts with supplementary information. Although their main purpose is to be used for the safe navigation of ships, they also contain much information on coasts and seas which may be interesting to ordinary people. However, there is no easy way to access them because of their specialized data format, access method and visualization. This paper proposes m implementation of SVG for the access and services of ENCs. SVG(Scalable Vector Graphic) makes it possible to make use of Vector graphics for map services in basic internet browsing environment. Implementation of SVG for ENC applications by this research is free of special server side GIS mapping system and client side extra technology. The Implementation of SVG for ENC Applications can be summarized as follows: Firstly, SVG provides spatial information to possess searching engine to embody SVG map. Secondly SVG can provide high-quality vector map graphics and interactive facility without special Internet GIS system. It makes it possible to use services with very low cost. Thirdly, SVG information service targeting on maritime transportation can be used as template, so it can be used dynamically any other purpose such as traffic management and vessel monitoring. Many good characteristics of SVG in mapping at computer screen and reusability of SVG document provide new era of visualization of marine geographic information.