• Title/Summary/Keyword: Marine Food

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Inhibitory Activity of Intracellular Lipid Accumulation by Various Marine Extracts in HepG2 Cells (HepG2 cell에서 식용수산자원 추출물의 지질축적억제효과)

  • Kim, Byoung-Mok;Jung, Ji-Hee;Kim, Dong-Soo;Kim, Young-Myoung;Jeong, In-Hak
    • Korean Journal of Food Science and Technology
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    • v.44 no.3
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    • pp.362-366
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    • 2012
  • This study was performed to promote the effective utilization of edible marine resources and to develop functional food material from edible marine resource extracts for inhibiting lipid accumulation in liver. Edible marine resource extracts (ME) were prepared by hot water (MWE) and 80% ethanolic (MEE), and both the MWE and MEE extracts were assessed as to their cell cytotoxicity, and Oil Red O staining. Results demonstrated that ME showed no cytotoxic effects. However, treatment with MEE in the concentration of 1000, 500, and 250 ${\mu}g/mL$ significantly inhibited the lipid accumulation in HepG2 cells compared to MWE. Especially, among the 80% ethanolic extracts, Pagrus major, Larimichthys polyactis, Clupea pallasii, Octopus minor, Enteroctopus dofleini, Styela clava, dried sea mustard, and Enteromorpha intestinalis showed greater lipid accumulation inhibitory activity than the others.

Fatty Acid Profiles of Marine Benthic Microorganisms Isolated from the Continental Slope of Bay of Bengal: A Possible Implications in the Benthic Food Web

  • Das, Surajit;Lyla, P.S.;Khan, S. Ajmal
    • Ocean Science Journal
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    • v.42 no.4
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    • pp.247-254
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    • 2007
  • Marine bacteria, actionmycetes and fungal strains were isolated from continental slope sediment of the Bay of Bengal and studied for fatty acid profile to investigate their involvement in the benthic food-web. Fifteen different saturated and unsaturated fatty acids from bacterial isolates, 14 from actinomycetes and fungal isolates were detected. The total unsaturated fatty acids in bacterial isolates ranged from 11.85 to 37.26%, while the saturated fatty acid ranged between 42.34 and 80.74%. In actinomycetes isolates, total unsaturated fatty acids varied from 27.86 to 38.85% and saturated fatty acids ranged from 35.29 to 51.25%. In fungal isolates unsaturated fatty acids ranged between 44.62 and 65.52% while saturated FA ranged from 20.80 to 46.30%. The higher percentages of unsaturated fatty acids from the microbial isolates are helpful in anticipating the active participation in the benthic food-web of Bay of Bengal.

Recovery of serine protease inhibitor from fish roes by polyethylene glycol precipitation

  • Lee, Hyun Ji;Kim, Hyung Jun;Park, Sung Hwan;Yoon, In Seong;Lee, Gyoon-Woo;Kim, Yong Jung;Kim, Jin-Soo;Heu, Min Soo
    • Fisheries and Aquatic Sciences
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    • v.19 no.5
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    • pp.25.1-25.8
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    • 2016
  • The fractionation of serine protease inhibitor (SPI) from fish roe extracts was carried out using polyethylene glycol-4000 (PEG4000) precipitation. The protease inhibitory activity of extracts and PEG fractions from Alaska pollock (AP), bastard halibut (BH), skipjack tuna (ST), and yellowfin tuna (YT) roes were determined against target proteases. All of the roe extracts showed inhibitory activity toward bromelain (BR), chymotrypsin (CH), trypsin (TR), papain-EDTA (PED), and alcalase (AL) as target proteases. PEG fractions, which have positive inhibitory activity and high recovery (%), were the PEG1 fraction (0-5 %, w/v) against cysteine proteases (BR and PA) and the PEG4 fraction (20-40 %, w/v) against serine proteases (CH and TR). The strongest specific inhibitory activity toward CH and TR of PEG4 fractions was AP (9278 and 1170 U/mg) followed by ST (6687 and 2064 U/mg), YT (3951 and 1536 U/mg), and BH (538 and 98 U/mg). The inhibitory activity of serine protease in extracts and PEG fractions from fish roe was stronger than that of cysteine protease toward common casein substrate. Therefore, SPI is mainly distributed in fish roe and PEG fractionation effectively isolated the SPI from fish roes.

Detection of Marine Birnavirus(MABV) from Marine Fish in the Southern Coast of Korea (남해안 자연산 어류에서 Marine birnavirus(MABV)의 검출)

  • Yun, Hyun-Mi;Kim, Seok-Ryel;Lee, Wol-La;Jung, Sung-Ju;Oh, Myung-Joo
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.13-18
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    • 2008
  • Marine birnavirus(MABV) are well known fish pathogens in Asian countries such as Korea, Japan, and China. Prevalence of viral disease, geological distribution and host or reservoir of viruses were investigated from wild marine fishes in southern coast of Korea in 2003 and 2005. RT-PCR results showed that MABV were detected in 17 fishes(10.6%) from 160 fishes. Comparative analysis of the nucleotide sequences and the deduced amino acids of MABV genome from wild fishes were similar to reference strains of MABV and distinguished with IPNV strains.

Mercury and Methylmercury Levels in Marine Fish Species from Korean Retail Markets

  • Oh, Keum-Soon;Suh, Jung-Hyuck;Park, Seong-Soo;Paek, Ock-Jin;Yoon, Hae-Jung;Kim, Hee-Yeon;Lee, Jong-Ok
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.819-823
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    • 2008
  • Mercury and methylmercury concentrations were measured in 225 marine fish samples purchased from retail markets in 5 major Korean cities in 2005. The results showed that the concentrations of mercury and methylmercury ranged from 0.006-0.455 (mean=$0.054{\pm}0.025$) mg/kg and 0.004-0.120 (mean=$0.021{\pm}0.008$) mg/kg, respectively, in fish caught from the coastal waters of the Korean Peninsula. The concentrations in deep-sea fish including tuna ranged from 0.066-2.315 (mean=$0.095{\pm}0.640$) mg/kg for mercury and 0.027-0.897 (mean=$0.281{\pm}0.182$) mg/kg for methylmercury. The estimated weekly intakes (EWIs) of mercury and methylmercury reached 3.92 and 4.70% of the provisional tolerable weekly intakes (PTWI), respectively. Consequently, the levels of mercury and methylmercury in marine fish from current retail markets do not pose any significant health risks for Koreans.

Effects of Three Microalgae, Tetraselmis suecica, Chaetoceros calcitrans, and Phaeodactylum tricornutum on Larvae and Spat Growth of the Trumpet Shell Charonia sauliae

  • Kang, Kyoung-Ho;Seon, Seung-Cheon;Kim, Jae-Min;Zhuo, Liang Liang;Lim, Sang-Min;Kim, Hyeon-Jeong
    • The Korean Journal of Malacology
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    • v.25 no.1
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    • pp.35-40
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    • 2009
  • The trumpet shell Charonia sauliae is an endangered and valuable species with potential for aquaculture. For artificial propagation of C. sauliae, the effects of three different food microalgae on the development, growth, and survival rate of the larvae and spat were investigated. For the larval feeding experiments, we utilized six microalgae species as food sources, namely Pavlova lutheri, Tetraselmis suecica, Nannochloris oculata, Isochrysis galbana, Chaetoceros calcitrans, and Phaeodactylum tricornutum; for the larval and spat growth and survival experiments, we utilized T. suecica, C. calcitrans, and P. tricornutum. The results showed that the temporal digestion index (TDI) for the veliger larvae was significantly different for C. sauliae fed the different microalgae species (p < 0.05), that the T. suecica, C. calcitrans, and P. tricornutum cultivars were better suited for larval consumption (p < 0.05), and that the growth and survival of the larvae and spat were significantly influenced by food type, specifically P. tricornutum (p < 0.05). Further research is needed to evaluate the effects of other microalgae species, different algal concentrations, and biochemical composition on the growth and survival of C. sauliae.

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Antioxidant and Antihypertension Effects of Enzyme Hydrolysate from Hippocampus abdominalis (식용 빅벨리 해마(Hippocampus abdominalis) 유래 단백질 가수분해물의 항산화와 항고혈압 효능)

  • Je, Jun-Geon;Kim, Hyun-Soo;Lee, Hyo-Geun;Oh, Jae-Young;Wang, Lei;Rho, Sum;Jeon, You-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.2
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    • pp.127-133
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    • 2019
  • Seahorses have long been used as ornamental and medicinal products. The sea horse Hippocampus abdominalis has a beautiful color and unique shape and is also used for ornamental purposes and as a traditional medicine in China. This study examined the value of H. abdominalis as a health functional food or food additive. H. abdominalis was hydrolyzed using seven proteases: flavourzyme, neutrase, alcalase, trypsin, kojizyme, pepsin and protamex. The yields of all of the enzyme hydrolysates were higher than that of the aqueous extract. Of the enzymatic hydrolysates, seahorse Protamex hydrolysate (SHP) gave the highest yield and had excellent antioxidant and angiotensin-I converting enzyme inhibitory activities. It protected Vero cells against oxidative by 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH) and antihypertension in Spontaneously Hypertensive Rats. This study attempted to demonstrate H. abdominalis as a health functional food or food additive in the future.