• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2001.05a
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• pp.383-387
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• 2001

The sea flower begins at the water-sediment Interface. In the ocean basins, the sound velocity of the sediments at the interface vary from a few percent less than the sound speed in water just above the interface to somewhat greater. Marine sediments are unconsolidated; that is, the particles are not cemented of fused together. Samples feel like mud, muddy sand, sand, and so on. With the theoretical knowledge, the systematic research on the searching capability of Ultra Sonic Signal will be continued to identify the influence against the sea water subject. In this research, signal will be analyzed according to the influence range, power and sensitiveness of Ultra Sonic Generator. In addition, the radius of Ultra Sonic Signal will be included. The experimental field work will be executed at Nockdong, Pulkyo and other places well known as a habitat of Pan Shell.

• Development and Reproduction
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• v.18 no.1
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• pp.33-41
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• 2014

We investigated the process of yolk absorption in Korean rose bitterling, Rhodeus uyekii, and determined the changes in its morphometric characteristics. The R. uyekii from 1 days post hatching (DPH) to 21 DPH, the eye diameter (ED) was 5.4 at 5 DPH. Thereafter, the ED/total length (TL) ratio increased to 10.7 at 21 DPH (p<0.05). The yolk length (YL) decreased from 95.4 to 1.1 by 21 DPH, and this rate of decrease was greater than that for any other dimension (p<0.05). 12 morphometric dimensions/TL for the R. uyekii were measured at each sampling day from 21 DPH to 170 DPH. At just hatching, the average TL and BW were $6.1{\pm}0.09mm$ and $4.9{\pm}0.07mg$, respectively. At 53 DPH, the average TL was $12.9{\pm}0.28mm$ and the average BW was $14.7{\pm}0.72mg$; the total length growth equation was $TL=5.507e^{0.038t}$ ($R^2=0.916$). Further, the body weight growth equation was $BW=3.3647e^{0.0296t}$ ($R^2=0.9354$). The dimensions with regard to body depth showed the greatest growth rates in the external characteristics of the fish (p<0.05). The patterns of the morphometric characteristics measured in this study can be classified in three ways. The patterns were shown to be increased (y=0.0992x+12.07, $R^2=0.8333$), decreased (y=-0.0569x+42.029, $R^2=0.8395$) or maintained (y=0.005x+18.263, $R^2=0.3678$) from 21 DPH to 170. These results will provide useful indices for the successful rearing of the R. uyekii.

• Development and Reproduction
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• v.18 no.1
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• pp.13-23
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• 2014

This study is conducted to monitor the morphological developmental features of the egg development, larvae and juvenile of Epinephelus septemfasciatus, the fertilized eggs were gotton using artificial insemination. Matured parents are collected from marine caged fish farms in Geomun-ri, Samsan-myeon, Yeosu-si, Jeollanamdo Korea in June 2012. The fertilized eggs were pelagic eggs containing one oil globule, and measured 0.81~0.89 mm ($0.85{\pm}0.04mm$, n=50) in diameter. In regard to rearing environment, the water temperature is $21.0{\sim}23.0^{\circ}C$ and the salinity is 32.0~33.2‰. Hatching was observed from 48 hours after fertilization, the mouth and anus of prelarvae was not opened but had egg yolk at newly hatched. 4 days after hatching, the mouth and anus of postlarvae was opened and began to eat Rotifer and was measured 2.40~2.49 mm ($2.45{\pm}0.03mm$ n=10) in total length. 12 days after hatching, postlarvae was measured 3.77~4.67 mm ($4.27{\pm}0.33mm$) in total length, its the second pole tide of dorsal fin and the first pole tide of pelvic fin was extended longitudinally. 71 days after hatching, juvenile was measured 40.5~45.4 mm ($42.6{\pm}2.04mm$) in total length. Seven bands were observed in body, and pole tides of dorsal and pelvic fins were shortened.

• Development and Reproduction
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• v.23 no.4
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• pp.367-375
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• 2019

Pufferfish (Takifugu spp.) are economically important edible marine fish. Mistakes in pufferfish classification can lead to poisoning; therefore, accurate species identification is critical. In this study, we used the mtDNA cytochrome c oxidase subunit I gene (COI) to design specific primers for six Takifugu species among the 21 domestic or imported pufferfish species legally sold for consumption in Korea. We rapidly and simultaneously identified these pufferfish species using a highly efficient, multiplex polymerase chain reaction (PCR) system with the six species-specific primers. The results showed that species-specific multiplex PCR (multiplex species-specific polymerase chain reaction; MSS-PCR) either specifically amplified PCR products of a unique size or failed. MSS-PCR yielded amplification fragment lengths of 897 bp for Takifugu pardalis, 822 bp for T. porphyreus, 667 bp for T. niphobles, 454 bp for T. poecilonotus, 366 bp for T. rubripes, and 230 bp for T. xanthpterus using the species-specific primers and a control primer (ca. 1,200 bp). We visualized the results using agarose gel electrophoresis to obtain accurate contrasts of the six Takifugu species. MSS-PCR analysis is easily performed and provides identification results within 6 h. This technique is a powerful tool for the discrimination of Takifugu species and will help prevent falsified labeling, protect consumer rights, and reduce the risk of pufferfish poisoning..

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.2
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• pp.107-117
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• 2012

Korean large purse seine fishery catches chub mackerel, sardine, jack mackerel, Spanish mackerel, etc. which are mainly pelagic fish species. The proportion of chub mackerel was 60% over in Korean large purse seine fishery. Sea surface temperature (SST) increased $0.0253^{\circ}C$ per year and total rising rate was $0.759^{\circ}C$ from 1980 to 2009 in the southern sea of Korea, where is mainly fishing grounds of Korean large purse seine. It was that p<0.01 level was statistically significant. It is northward movement that the center of fishing grounds of chub mackerel by Korean large purse seine fishery moved 4.57km/yr. It was rapidly northward movement about 7.1km/yr, 8.13km/yr to move Spanish mackerel and bluefin tuna fishing grounds. However, the fishing grounds of jack mackerel were moved further south in the 2000s than the 1980s. Catch of tunas and bluefin tuna consistently increased in Korean waters. There was a significantly positive correlation between SST and catch of bluefin tuna in the fishing grounds of Korean waters.

• Development and Reproduction
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• v.19 no.4
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• pp.209-215
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• 2015

Red spotted grouper, Epinephelus akaara is a popular aquaculture species in many Asian countries. This species is a protogynous hermaphrodite that first differentiates into female and changes to male later. Due to this reproductive characteristic, stable supply of male and female gametes is a key to the success of seed production in this species. Thus, understanding early gonadal differentiation is required to develop effective sex control techniques. Red spotted grouper were reared in indoor tanks and sampled every 5 days from 40 days post-hatch (DPH) to 130 DPH. Changes of gonadal tissues were examined and analyzed by means of histology. A pair of gonadal primordium has already existed underneath the kidney in the posterior part of the body cavity at 38 DPH when this study began. Gonadal primordia of 38, 40 DPH consisted of germ cells surrounded by a few somatic cells. The blood vessel was observed in the gonadal primordium at 45 DPH. The number of somatic cells and size of gonadal primordium increased age-dependently up to 60 DPH. Formation of ovarian cavity was obvious by two protuberant aggregations of somatic cells at 65 DPH. Completed ovarian cavity and oogonia were first observed in the gonad of one fish sample at 105 DPH. Based on these histological observations, it can be suggested that induction of primary male differentiation could be more successfully applied at around 60 DPH in this species.

• Applied Microscopy
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• v.29 no.4
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• pp.417-426
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• 1999

The present investigation is designed to provide basic information on fine structure of the skin of the sea bass, Lateolabrax japonicks in relation to study of epidermal change with environmental and physiological change. The skin of the sea bass is divided into the epidermal layer and dermal layer. Epidermal layer consists of supporting cells and unicellular glands. The supporting cells were classified into the superficial cell, intermediated cell and basal cell. Gland cells were classified into the mucous secretory cell and club cell which is more frequently observed. Superficial cell of epidermal layer is squamous or cuboidal and contains well-developed rough endoplasmic reticulum and the surface is covered with numerous microridges. Superficial cells are connected to another cell with membrane interdigitations and desmosomes. Intermediated cell is ovoid and the electron density is higher than the other supporting cells. Basal cell is cuboidal and has a well-developed mitochondria and membrane interdigitation. The mucous secretory cell has a numerous membrane bounded secretory granules. The cytoplasm of club cell is divided into cortex and medullar. The medullar cytoplasm has a nucleus, intracellular organelles and central vacuole, and the cortical cytoplasm has a well-developed tonofilament. Club cells are connected to another cell with well -developed membrane interdigitations and desmosomes.

• Applied Chemistry for Engineering
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• v.19 no.5
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• pp.491-496
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• 2008

The microsomal epoxide hydrolase gene (referred to as mMCEH) of Mugil cephalus was cloned by PCR, and then inserted to pColdI and pET-21b(+) vector, respectively. The recombinant E. coli possessing the recombinant plasmids exhibited the enantioperference toward (R)-styrene oxide. When enantioselective kinetic resolutions were conducted with 20 mM racemic styrene oxide, enantiopure (S)-styrene oxide was obtained with high enantiopurity more than 99% enantiomeric excess (ee) and 24.50% yield by using the recombinant E. coli harboring pET-21b(+)/mMCEH.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.43 no.4
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• pp.347-354
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• 2007

This paper describes on the real-time monitoring of net setting and hauling process for fishing operations of Danish seine vessels in the southern waters of Korea as an application of a PC based ECDIS system. Tracking of fishing process was performed for the large scale Danish seine vessel of G/T 90 and 350 PS class using the fishing gear which the length of net, ground rope, head rope and sweep line including warp in both sides were 86m, 104m, 118m and 3,200m, respectively. Tracking information for net setting and hauling process was continuously recorded for 23 fishing operations performed on November and December, 2003. All measurement data, such as trawl position, heading, towing course and past track which was individually time stamped during data acquisition, was processed in real time on the ECDIS and displayed simultaneously on the ENC chart. The results indicated that after the separation of a marker buoy from Danish seiner, the averaged running speed of vessel and the averaged setting period while shooting the seine on the course of diamond shape to surround the fish school in the 23 fishing operations were 8.3 knots and 13.1 minutes, respectively. And with the maker buoy taken on board, the averaged running speed of vessel and the averaged towing period while closing the seine on the straight route was 1.0 knots and 47.0 minutes, respectively. After the closing stage of hand rope, the hand rope was towed by the averaged speed of 2.2 knots during the 13.0 minutes. The average area for route of diamond shape swept by sweep lines of the seine in 23 fishing grounds was $709,951.6m^2$. Further investigation is also planed to provide more quantitative tracking information and to achieve more effective surveillance and control of Danish seine vessels in EEZ fishing grounds.

• Fisheries and Aquatic Sciences
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• v.10 no.1
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• pp.30-36
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• 2007

We present age and growth data for Ilisha elongata, based on 363 specimens caught in 1999 and 2000 around Jeju Island and the southwest coast of Korea. Scale and ring radii of each ring group showed a direct one-to-one relationship, with ring radius increasing as scale radius increased. The relationship between fork length and scale radius was FL=39.553SR+60.935 for females, FL=39.474SR+54.026 for males, and FL=39.608SR+57.547 for both sexes. Monthly changes in the marginal index indicated that a new ring appears once each year (in July). Maximum age, mean age and mean fork length of fish were 10 years, 5.0 years, and 349mm, respectively. Von Bertalanffy's growth equations were $FL_t=495.4{\times}[1-\exp(-0.2586(t+0.6487))]$ for fork length (mm) and $W_t=1,112.5{\times}[1-\exp(-0.2586(t+0.6487))]^{2.874}$ for body weight (g) for both sexes. Monthly changes in the gonadosomatic index (GSI) indicated that the spawning period was in June and July, which corresponded closely with ring formation time. Differences in monthly GSI changes in 1999 and 2000 may be related to water temperature variation. Fecundity ranged from 5,664 to 176,290 eggs per individual (n=43, fork length 264-470mm). The relationship between fecundity(F) and fork length was $F=7.7{\times}10^{-7}{\times}FL^{4.9269}$.