• The Korean Journal of Malacology
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• v.24 no.2
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• pp.131-136
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• 2008

Growth and survival of the spat of arkshell, Scapharca broughtonii were investigated for the improvement of survival in intermediate culture with different shape of protective net and type of preventive net of spat loss. The growth performance of the spat of arkshell was observed for 60 days by using different forms of protective net, as the exposure form with an average shell length of $12.8{\pm}3.2$ mm(P<0.05), the fish pot form with $12.2{\pm}3.5$ mm, the cylinder form with $11.9{\pm}3.8$ mm and the last one is the rectangular form with $10.9{\pm}3.7$ mm. Their numerical value did not show any marked difference with each other. But in case of survival rate, the fish pot form showed highest survival rate which is 43.7% and the significantly lowest was showed by the exposure form, which is 5.4%(P<0.05). The cylinder form and rectangular form showed 41.2 and 31.6% respectively. And then the growth of the spat of arkshell was observed for 163 days by rearing in a sort of preventive net, the first group was in a blackout curtain with average shell length of $13.9{\pm}3.1$ mm, the next group was in a balsam pear net with $12.9{\pm}3.0$ mm, in a polyethylene net with $11.8{\pm}3.1$ mm and the control with $12.6{\pm}3.3$ mm which was not installed by preventive net of spat loss. The survival rate was 91.5% in a blackout curtain, 90.1% in a polyethylene net, 88.5% in a balsam pear net and 61.5% in a controlled group. It is seen that the highest growth and survival rate were observed in a fish pot form and cylinder form. These results were a little bit difference from those of the spat of arkshell cultured in the form of different preventive nets. There was no significant difference in survival rate in relation to the sorts of preventive net against carrying-away, but there was a difference in survival rate by more than 30% as against the non-installed controlled group. We expect that survival rate would be highly improved in intermediate culture carried out with fish pot form and cylinder form of protective net and preventive net of spat loss.

• Journal of the Korean Society of Food Culture
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• v.26 no.6
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• pp.583-598
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• 2011

This study was intended to outline the characteristics of the food culture in the area of Kyungsangnamdo and its modernization by interpretation and analysis of the novel Toji, which was set in Hadong, Jinjoo in the area of Kyungsangnamdo in the early 20th century. The characteristics of the Kyungsangnamdo area's native dish during the Japanese ruling era in the latter half of the Choson dynasty are as follows. In the first part of the novel, which spans from 1897 to 1908, vegetable and grain food development can be seen in the area of Hadong, the interior plains of Kyungsangnamdo, where there is a typical farming village in the mountains. The second part of the novel, which spans from 1911 through 1917, includes some mentions of the properties of Kyungsangnamdo area's native dishes through the lens of emigrated Koreans living on Gando island. Gando island is in China, and is where Seohee, the heroine, escapes from her homeland and remains for a period of years. There is a unique type of seafood in the Gando area using fresh marine products, exactly the same as in the Kyungsangnamdo area. The third part of the novel spans 1919 through 1929, after Seohee returns to her own country and regains her house. There is a noticeable description of food culture in the area of Jinjoo in Kyungsangnamdo through the description of Seohee focusing on the education of her children. The well-described features of Jinjoo are boiled rice with soup of beef leg bones and Jinjoo bibimbob, with vegetables and a variety of foods using cod. Cod are caught in large quantities in Kyunjgsangnamdo, and cities in the area grow to medium size as the area became traffic-based. The fourth part of the novel spans from 1929 through 1938, and includes very detailed descriptions of characters and background locations. Salted fish combined with the wild ingredients of Mt. Jiri feature prominently in the Kyungsangnamdo's area descriptions. The fifth part spans from 1940 through 1945, and as the Japanese colonization era ends, the foods described in Kyungsangnamdo seem to develop the usage of soybean paste. With abundant fish and shellfish Kyungsangnamdo, the dishes that evolve to use soybean paste include mussel soybean paste soup, picked bean leaves in soybean paste, chaitgook - cold soup from soybean paste, and seolchigook used with seaweed and sea laver.

• Journal of fish pathology
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• v.28 no.2
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• pp.79-85
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• 2015

A specimen of porous-head eelpout Bothrocara hollandi (Zoarcidae: Perciformes) caught from the East Sea was found to harbour a myxosporean parasite. Numerous whitish pseudocysts were scattered throughout the body musculature of this individual specimen. Fresh myxosporean spores were found from the squashed pseudocysts under light microscopy. They were subspherical in frontal view with a length of $11.9(11.0{\sim}13.5){\mu}m$, width of $11.6(10.7{\sim}13.6){\mu}m$, and thickness of $7.8(6.9{\sim}8.8){\mu}m$. Two polar capsules were almost equally pyriform with a length of $4.4(3.2{\sim}5.3){\mu}m$ and width of $3.3(2.4{\sim}4.2){\mu}m$. Morphometric and host ecology analysis revealed that this myxosporean parasite could be identified as Myxobolus aeglefini Auerbach 1906. Phylogenetic analysis based on 18S rDNA sequences also revealed that M. aeglefini was clustered with M. albi and M. groenlandicus in the same branch, sharing 97.7% and 96.9% sequence similarities with M. albi and M. groenlandicus, respectively.

• Korean Journal of Food Science and Technology
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• v.47 no.6
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• pp.719-724
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• 2015

The bactericidal effects of strongly acidic hypochlorous acid water (StAHA) and slightly acidic hypochlorous acid water (SlAHA) against Vibrio parahaemolyticus contaminated on surface of raw fish and shellfish were examined. V. parahaemolyticus contaminated with about 7.0 log CFU/g on the meat chunk of olive flounder (Paralichthys olivaceus), and yellow tail (Seriola quinqueradiata), and 4.0 log CFU/g on the shucked scallop (Patinopecten yessoensis) were not detected after washing with StAHA and SlAHA at a ratio of 30:1 on a sample weight basis. However, 1.0 log CFU/g of V. parahaemolyticus was survived on shucked oyster (Crassostrea gigas) under same treatment conditions. The bactericidal effects of acidic hypochlorous acid water against V. parahaemolyticus contaminated on surface of shucked oyster were not as effective as those against V. parahaemolyticus contaminated on surface of meat chunk of olive flounder, yellow tail, and shucked scallop. Such differences can be attributed to the complicated surface conformation of oyster.

• Journal of Life Science
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• v.13 no.1
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• pp.128-135
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• 2003

102 ESTs (Expressed Sequence Tags) were obtained by sequencing clones from a library of rainbow trout kidney cDNAs. Of the sequences generated, 55.8% of the ESTs were represented by 37 known genes. The 45 clones of unknown gene products potentially represent 40 novel genes. The genes involved in structural function (14.5%) and transcription/translation (11.6%) account for the major gene expression activities in the kidney Microarray experiment was conducted to compare gene expression of the unique ESTs in young and adult rainbow trout kidneys. While mitochondrion, cytochrome b, rho G, spastin protein, and three unknown genes were down-regulated in the mature fish kidney, calponin 1, calcium binding protein, histone deacetylase 1, and an unknown gene were up-regulated in the mature fish kidney. This research demonstrates the feasibility and power of functional genomics in rainbow trout.

• Journal of Aquaculture
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• v.1 no.1
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• pp.1-11
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• 1988

There are many problems to be solved for the establishment of more feasible and simplified method of seed production of marine fishes. One of the most important tasks in seed production is to clarify the feeding ecology of larvae and juveniles under. rearing conditions. In the present study, two groups of postlarvae-juveniles of the flounder, Paralichthys olivaceus were used. One ranging 11.38 to 17.08 mm in mean total length was fed with Artemia nauplii at two different water temperatures (19 to $21^{\circ}C$ and 24 to $25^{\circ}C$) and the other ranging 4.39 to 10.64 cm in mean total length was fed with prepared diet. Time required from the start of feeding to satiation was 45 to 55 minutes at 24 to $25^{\circ}C$ and nearly one hour at 19 to $21^{\circ}C$. Mean numbers of Artemia nauplii in digestive tract were 236 to 375 individuals per fish at 24 to $25^{\circ}C$. The amount of food remained in the intestine was $24.8\%$ of the total food in the digestive tract. In juveniles of 4.39 to 10.64 cm in mean total length, amount of prepared diet consumed at satiation was 0.04 to 0.46 g per fish. Digestive tract index in juveniles was rapidly decreased for 24 hours after satiation and kept nearly constant after 48 hours, showing that the time to completely digest the prepared diet in juveniles was estimated to be about 48 hours.

• Korean Journal of Animal Reproduction
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• v.23 no.4
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• pp.303-311
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• 1999

Nuclear DNA was isolated from the sperm cells representing genetic characteristics and genomic polymorphisms of rainbow trout by polymerase chain reaction(PCR) amplification of DNA using arbitrary primers. Genomic DNA fingerprints were generated from rainbow trout sperm DNA by polymerase chain reaction amplification using 20 arbitrary decamers as primers. Out of these primers, 4 generated 17 highly reproducible RAPD markers, producing almost six polymorphic bands per primers. Four of 6 primers tested generated amplified fragments which were polymorphic between different individuals. Polymorphic DNA fragments were reproducibly amplified from independent DNA preparations made from individuals. Rainbow trout was distinctly observed 3 specific DNA markers (2. 3, 2.0 and 1.3kb) in bandsharing. Individual fragments generated using the same arbitrary primer, demonstrated that a single primer detected at least three independent genomic polymorphisms in rainbow trout sperm DNA. The RAPD polymorphism generated by this primer may be used as a genetic marker for individual identification The RAPD-PCR technique has been shown to reveal informative polymorphism in many species of fish. The present results demonstrate that RAPD markers are abundant, reproducible and provide a basis for future gene mapping and MAS in these important aquaculture species using RAPD polymorphic markers. It is concluded that RAPD polymorphisms are useful as genetic markers for fish breed differentiation.

• Journal of fish pathology
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• v.1 no.1
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• pp.31-37
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• 1988

An experiment was carried out in order to reveal the infection species of trematode and the infection demage in the marine bivalves. Metacercaria of Echinostomatidae were found in Mactra veneriformis, Cyclina sinensis and Solen strictus in the vicinity sea of Naecho-do, the estuary of the Keum River in the western coast of Korea. The metacercaria detected from Solen strictus were fed by Larus crassiostris, at 16 days after infection, the adult worm could be seceded. The metacercaria of Himasthla kusasigi were detected from Mactra veneriformis and Cyclina sinensis, their infection rates were 70.4% and 85.7% respectively. The partial infection rates with respect to the body parts were 40.4% and 77.3% in the foot, 17.0% and 12.4% in the gill, 12.6% and 10.3% in the mantle in order. The metacercaria of Acanthoparyphium were found from Solen strictus. The total infection rate was 63% and the partial infection rate was 63% and the partial infection rate was shown foot, mantle and gill in order. The adult worm ceded from Larus crassiostris was indentified as Acanthoparyphium tyosenensis Yamaguti, 1938 from view point of its physical characteristics. Therefore, Mactra veneriformis, Cyclina sinensis would be added as new intermediate hosts, and Echoinostoma decteded from Mactra sulcataria which was studied by author(1969) was revealed as larvae of Acanthoparyphium tyosenensis.

• Journal of fish pathology
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• v.20 no.3
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• pp.269-279
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• 2007

This study was conducted to investigate the change of antioxidant enzyme activity (catalase and superoxide dismutase) and variation of blood physiology in olive flounder (Paralyticus olivaceus) by hydrogen peroxide (H2O2) treatment. Blood parameters were measured 1, 3 and 5 hours after H2O2 treatment with 0 (control), 100, 300 and 500 ppm for 1 hr. The value of hematocrit was decreased significantly dependently on treatment concentrate and elapsed time in the treatment of H2O2. Hemoglobin concentration in the test groups were lower than that of the control group. Red blood cell value in the test groups were significantly lower compared to that of the control group, but recovered to the level of the control group after 5 hr. Protein concentration was significantly lower compared to that of the control group at 0 and 1 hr, but recovered after 3 hr in 500 ppm treatment group. The superoxide dismutase (SOD) and catalase (CAT) enzyme activities were observed to be increased. Heat-shock protein 70 (HSP70) was significantly increased compared to that of control group in all of the test groups. HSP70 mRNA groups was highly expressed in 500 ppm treatment.

• Journal of fish pathology
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• v.23 no.1
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• pp.37-45
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• 2010

In this study, we carried out research on the level of single and multi-drug resistance of bacteria isolated from cultured flounder Paralichthys olivaceus. One hundred sixty one bacteria were isolated from cultured olive flounder Paralichthys olivaceus in Korea and the isolates consisted of Edwardsiella tarda (n=32), Vibrio ichthyoenteri (n=37), Vibrio spp. (n=54), Streptococcus parauberis (n=28) and Streptococcus spp. (n=10). These E. tarda isolates were highly resistant in the order of tetracycline (84.4%) and oxolinic acid (71.9%). V. ichthyoenteri and Vibrio spp. showed resistance ampicillin (94.6% and 81.5%) and tetracycline (56.8% and 42.6%). S. parauberis isolates were resistant ampicillin (57.1%), tetracycline (57.1%) and erythromycin (35.7%). Of the isolates, 84.4% of E. tarda, 73.0% of V. ichthyoenteri, 57.4% of Vibrio spp., 42.8% of S. parauberis and 70.0% of Streptococcus spp. isolates exhibited multi-drug resistance against more than two antibiotics.