• 한국항해항만학회:학술대회논문집
• /
• 한국항해항만학회 2009년도 공동학술대회
• /
• pp.498-501
• /
• 2009

국민의 여가시간 증대로 친수시설에 대한 해양관광 수요의 증가가 예상되며, 이에 주요 항만이나 지역의 어촌, 경승지 및 명소에 어울리는 조형미를 갖춘 등대시설을 관광자원화하여 이러한 수요를 충족시킬 필요가 있다. 또한 우리나라에 등대가 들어선지 어언 100여년이 지나면서 훼손된 등대를 새롭게 신축하고 있는 시점에서 우리나라 등대건축을 진단하고 그것들의 보존과 적극적인 해양관광자원으로서의 활용방안 연구가 필요하다. 이제 등대는 전통적인 기능과 역할에 충실한 항로표지일 뿐만 아니라 온 국민이 향유하고 체험하는 새로운 관광시설이자 공간으로 적극 개발되어야 할 것이다. 이러한 맥락에서 본 연구는 국내 등대 개발사례를 검토하여 등대의 해양관광자원으로서의 개발을 위한 방안을 제시하였다.

• 한국수산과학회지
• /
• 제44권6호
• /
• pp.665-670
• /
• 2011

The rotifer Brachionus plicatilis is one of the most important food organisms in aquaculture. The resting eggs produced by mictic female rotifers are easily stored and hatched, making them useful as the starter for the mass culture of rotifers in marine larval culture. This study examined the optimum preservation method for resting eggs to ensure a high hatching rate. To produce resting eggs, the marine rotifer B. plicatilis was cultured with Nannochloris oculata (KMMCC 16). The resting eggs were harvested and cryopreserved using 5% and 10% methanol (MeOH), dimethylsulfoxide (DMSO), and glycerol as cryoprotectant agents (CPAs). The cryopreservation comprised slow or rapid freezing and the resting eggs were stored for one month in liquid nitrogen ($-196^{\circ}C$). The resting eggs were also dried at different temperatures (30, 40, and $50^{\circ}C$) and for different times (1, 2, and 3 h). In general, the hatching rates of the resting eggs preserved with CPA were higher than those without CPA and the slow freezing method was better than the rapid freezing method. However, the optimum CPA concentration for the hatching rate of the resting eggs varied with the freezing method and kind of CPA, and the CPA also affected the viability of the resting eggs. Dried resting eggs had a high, rapid hatching rate over 80%. The moisture content of the resting eggs cryopreserved in liquid nitrogen affected the hatching rate. Drying at $30^{\circ}C$ for 1 hour resulted in a high hatching rate of the resting eggs. In conclusion, drying at $30^{\circ}C$ for 1 hour and preservation in liquid nitrogen with the slow freezing method, without CPA, is recommended for a high hatching rate (ca. 95%) of rotifer resting eggs.

• 한국수산과학회지
• /
• 제44권6호
• /
• pp.658-664
• /
• 2011

Rotifers of the genus Brachionus are commonly used as a live food for larval fish, and rotifers of different sizes are preferred according the mouth size of the fish. Rotifer species vary in size, and individual size can depend on the temperature and salinity of the rearing environment. We investigated the effects of temperature and salinity for two species, B. plicatilis (250-300 ${\mu}m$) and B. rotundiformis (100-220 ${\mu}m$). Two strains of B. plicatilis (CCUMP 36 and 48) and two strains of B. rotundiformis (CCUMP 51 and 56) were received from the Culture Collection of Useful Marine Plankton (CCUMP) at Pukyong National University and cultured with the green alga, Nannochloris oculata (KMMCC 16) from the Korea Marine Microalgal Culture Center (KMMCC). The growth and size of rotifers were examined at three water temperatures ($16^{\circ}C$, $24^{\circ}C$, $32^{\circ}C$) and four salinities (20 psu, 25 psu, 30 psu, 35 psu) under continuous light (40 ${\mu}molm^{-2}s^{-1}$). The maximum density and growth rate of B. rotundiformis were greater than those of B. plicatilis. The lorica length of B. plicatilis ranged from 215.4 to 269.7 ${\mu}m$ and from 154.9 to 206.6 ${\mu}m$ for B. rotundiformis, depending on strain, temperature and salinity. Rotifers were smaller when cultured at high temperatures, regardless of salinity. B. rotundiformis preferred higher salinity than B. plicatilis. The results demonstrated that the size of rotifers could be controlled to some extent by temperature and salinity.

• ALGAE
• /
• 제30권sup호
• /
• pp.1-188
• /
• 2015

• Journal of Microbiology and Biotechnology
• /
• 제16권12호
• /
• pp.1947-1953
• /
• 2006

There have been a number of studies of methods for recycling animal wastewater to provide new bioresources. In the present work, a marine algal culture medium, designated KEP II, was prepared by adding swine waste (3% v/v) fermented by soil bacteria to a dilution of f/2 culture medium (CT). When Phaeodactylum tricornutum was grown in batch culture in KEP II, the cells lasted long at the exponential phase producing the specific growth rate and biomass; the production of total amino acids and secondary metabolites rose up to 5-fold. It also substantially enhanced the maximum quantum yield of photo system (PS) II of P. tricornutum, greatly increased the level of thylakoid membranes containing PS, and stimulated the production of pyrenoids, including enzymes for $CO_2$ fixation in chloroplasts. KEP II should improve the cost efficiency of industrial mass batch cultures and the value of microalgae for long-term preservation of fresh aquaculture feed as well as production of anticancer and antioxidant agents. Specifically, a low-cost medium for growing the diatoms of aquaculture feed will be economically advantageous.

• 한국해양학회지:바다
• /
• 제23권3호
• /
• pp.109-124
• /
• 2018

실험실 내에서의 해양 선충류 최적 배양 조건을 찾기 위해 한천배지로 다양한 배지를 제작하여 선충류를 대상으로 한 연구를 수행하였다. 선충류 채집은 태안 만리포 조간대 중 하부의 사질 퇴적물 표층에서부터 약 5 cm의 퇴적물에서 이루어졌다. 배양에 이용한 한천 배지는 Killian 배지의 조성을 다소 변형하고, 한천 농도에 차이를 두었으며, 이 한천배지들을 직경 60 mm 페트리디쉬에 배지 높이가 2.0 mm가 되도록 분주하였다. 선충류의 먹이 생물로서 순수 배양한 미생물 5종을 혼합하여 각각의 배지에 첨가하였다. 각 배지에 Enoplolaimus sp. 5개체를 접종하여 $20^{\circ}C$, 암배양으로 설정된 배양기 내에서 배양하였다. Enoplolaimus sp.에 대하여 여러 조건의 실험을 반복 수행한 결과, Killian 배지에 소고기 육즙만을 첨가한 배지의 한천농도 1.0%에서 평균 생존시간이 246.8시간으로 가장 높은 생존율을 나타냈다. 가장 낮은 생존시간은 Killian 배지에 bacto peptone과 소고기 육즙(beef extract)을 첨가한 한천농도 0.4%의 먹이 공급을 한 배지에서 평균 27.6시간으로 가장 낮은 생존시간을 나타냈다. Bathylaimus sp.는 Killian 배지 한천농도 1.0%의 먹이 공급을 하지 않은 배지에서 가장 높은 생존시간을 나타내었고, 산란량과 부화율 실험에서는 평균 7개의 알을 산란하였으며 평균 99.5시간 후에 94.7%의 부화율을 보이는 결과를 나타냈다

• 해양환경안전학회지
• /
• 제30권1호
• /
• pp.20-31
• /
• 2024

본 연구는 패류양식업이 밀집한 남해 강진만 해역에서 수하식 및 살포식 양식해역과 비양식해역을 구분하여 양식활동으로 인한 유기물 축적과 저서다모류군집 변화를 파악하고, 어장환경평가 기법을 활용하여 건강도를 평가하고자 수행되었다. 남해 강진만 해역의 평균 입도, 수온, 염분농도, 총유기탄소 등의 시·공간 분포는 정점간에 큰 차이가 없었던 반면, 출현 종수, 개체밀도와 종다양도 등은 살포식 양식 해역의 정점이 다른 해역의 정점과 비교해 상대적으로 낮은 경향을 보였다. 한편 집괴분석과 주요좌표분석 결과에서도 살포식 양식해역 정점의 저서다모류군집이 나머지 정점의 군집과 뚜렷하게 구분되었다. 수하식 양식해역의 정점과 비양식해역 정점에서는 오염지시종인 Scolectoma longifolia와 Sigambra tentaculata가 우점종으로 출현하였으나, 수심과 해수 유통 등의 물리적인 요인에 의한 영향을 받는 일부 정점을 제외하면 점유율이 높지 않았다. 강진만 해역의 저서생태계 건강도는 1~2등급으로 양호한 상태였다. 그러나, 다모류군집구조의 시·공간 분포와 저서생태계 건강도지수를 고려하면 살포식 양식해역의 퇴적환경은 양식으로 인한 물리적인 교란에 영향을 받는 것으로 보여진다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVII)
• /
• pp.343-346
• /
• 2005

The isolated strain, SC2-1 was Gram-positive, catalase positive, facultatively anaerobic, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and sodium chloride required for the bacteria growth. The radical scavenging activity of the culture supernatants was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) method. This bacterium was identified based on cellular fatty acids analysis and 16S rDNA sequencing then named Exiguobacterium sp. SC2-1. The optimum culture conditions for production of antioxidant were $25^{\circ}C,$ pH 7.8 and NaCl concentration were 4%. The modified optimal medium compositions were maltose 2.5% (w/v), yeast extract 1.5% (w/v) and $KH_2PO_4$ 0.05% (w/v). Free radical scavenging activity of under optimal culture conditions were 93%.

• ALGAE
• /
• 제24권4호
• /
• pp.205-212
• /
• 2009

Molecular genetic tools are widely used to learn more about the identical characterization of obscure microalgal strains. At the Korea Marine Microalgae Culture Center (KMMCC), the authors deduced the genetic relationship of 41 strains of the genus Tetraselmis by analysing a small subunit ribosomal DNA (18S rDNA) sequences. Forty-one strains were seperated into five groups, which showed over a 98-99% similarity to Tetraselmis striata or Tetraselmis sp. Tsbre. Also, 13 strains among them had an identical genotype to Tetraselmis striata while 5 strains had with Tetraselmis sp. Tsbre, respectively. The mean size of each strain generally showed the tendency of different variation according to the groups.

• KSBB Journal
• /
• 제14권3호
• /
• pp.351-357
• /
• 1999

A marine bacterium Bacillus cereus ASK202, agarase producing strain, was treated with some mutagenic agents, ultraviolte(UV), 1-methyl-3-nitro-1-nitrosoguanidine(NTG), and ethyl methane sulfonate(EMS), several times for the increasing of the agarase production After mutagen treatment, we isolated one mutant strain treated with NTG showed the highest stability and agarase productivity and named as Bacillus cereus ASK202-N3. This Bacillus cereus ASK202-N3 strain was well grown in the modified marine medium containing 0.5%(w/v) agar, 0.3%(w/v) yeast extract, and 5.0%(w/v) NaCl, and the optimal initial pH, temperature and culture time were 7.8, $25^{\circ}C$ and 32h, respectively. In the optimal culture conditions, the agarase production was increased to 5.3 fold(850units/L) compared to that of the wild type.