• Korean Journal of Veterinary Research
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• v.41 no.4
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• pp.565-570
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• 2001

The diagnosis of swine sarcoptic mange (Sarcoptes scabiei var. suis) was investigated by ELISA in order to replace current diagnostic methods such as skin scraping, scratching index, or lesion score of dermatitis. The current methods need many efforts and much times and cost much. They can not handle many samples simultaneously. Therefore, in this research we developed ELISA that can handle many samples at a time. The antigens of swine sarcoptic mite were isolated and examined by 12.5% SDS-PAGE and silver staining. The antigenicity of antigen was confirmed by Western blotting using the swine from the artificailly-infested swine with swine sarcoptic mite. The optical density (OD) values of the artificailly-infested positive sera and the naturally-infested positive sera of sows were measured and read in order to confirm the stability of antigens, the reproducibility and validity (sensitivity and specificity) of the manufactured ELISA of swine sarcoptic antigens. In above results, the developed ELISA would be possible to use the diagnostic tool of sarcoptic mange if OD values of piglets, fattening pigs and sows are interpreted reasonably and classified as mange-free and infested.

• Journal of Biosystems Engineering
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• v.30 no.5 s.112
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• pp.306-311
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• 2005

In this study, a biosensor was developed to rapidly diagnose the swine sarcoptic mange (Sarcoptes scabies var. suis). The ELISA was modified to reduce the processing time for rapid diagnosis. The biosensor consists of a biological reaction part, and a measurement and control part. The biological reaction part was designed for using micro-pumps and valves for fluid transportation, and the measurement control part composed of a photodiode, a light-emitting diode fur light measurement, and a microcomputer to implement assay A polystyrene covet was used as a reaction chamber. Signal output was read as the rate of change in optical density at 645nm. Eighteen pigs diagnosed with sacroptic mange and 19 control pigs were tested. Fifteen sacbies-infested pigs showed positive results ($83.3\%$ sensitivity). Sixteen control pigs showed negative results ($84.2\%$ specificity). The system could execute a diagnosis cycle in about 45 min. The results suggest that this biosensor is useful for the rapid diagnosis of swine sacroptic mange.

• Parasites, Hosts and Diseases
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• v.60 no.6
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• pp.423-427
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• 2022

The long-tailed goral, Naemorhedus caudatus (Mammalia: Bovidae), is one of the endangered animals in the Republic of Korea (Korea). Sarcoptic mange mites infested in diverse species of mammals, including humans, but no case has been reported in long-tailed gorals. We report 2 cases of mange mite, Sarcoptes scabiei, infestation in longtailed gorals. Mange mites were sampled in the skin legions of the 2 long-tailed gorals, which were rescued in 2 different regions, Uljin-gun, Gyeongsangbuk-do and Cheorwon-gun, Gangwon-do, Korea. Our results showed that the ectoparasite was the itch mite that burrowed into skin and caused scabies on the morphological inspection and placed within the phylogenetic relations of the species. The present study confirmed for the first time in Korea that mange mites are pathogenic scabies of long-tailed goral. Closer surveillance of this pathogenic ectoparasite in zoonotic and infectious ecosystems is warranted.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.2
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• pp.165-168
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• 1999

To determine the effects of genetic and environmental influences on cell mediated immune (CMI) responses in broiler rabbits, contact sensitivity to 2,4-Dinitrochlorobenzene (DNCB) was assessed in three temperate broiler breeds of rabbits, namely Soviet Chinchilla, New Zealand White and Grey Giant. The feasibility of using the contact sensitivity to DNCB as a marker trait in selection for disease resistance was examined. There were highly significant differences between breeds (p<0.01) in initial skin thickness and contact sensitivities to DNCB at 24, 48 and 72 hours. Initial skin thickness was greatest in the Soviet Chinchilla breed (mean 2.2484 mm), and was significantly greater (p<0.01) in males (2.4963 mm) than in females (1.7846 mm) (p<0.01). Highest contact sensitivity to DNCB was in the New Zealand White breed with mean increase in skin thickness of 1.1884, 0.9072 and 0.5879 mm at 24, 48 and 72 hours post challenge respectively. Delayed type hypersensitivity (DTH) reaction to DNCB at 24 hours post challenge had a highly significant association (p<0.01) with the incidence of body mange in rabbits. The results indicated a lowered contact sensitivity to DNCB at 24 hours post challenge was associated significantly (p<0.01) with an increase in incidence and severity of body mange, suggesting its potential value as a marker. The correlation s among contact sensitivities at 24, 48 and 72 hours were positive and highly significant (p<0.01); correlations between initial skin thickness and contact sensitivities were negative and highly significant (p<0.01). Another notable significant correlation was between body weight and delayed type hypersensitivity at 24 hours indicating that an enhanced CMI might be associated with better growth rate and general wellbeing.

• Journal of the korean veterinary medical association
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• v.38 no.6
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• pp.549-558
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• 2002

3.5살짜리 래브라도 리트리버(Labrador Retriever)는 매일 처방되는 피질스테로이드제에 무반응으로 비계절적, 심한 소양증을 2년간 앓고 있는 병력을 갖고 있었다. 공기유래 알레르겐(알러젠; Allergens)들에 대한 시험관내 RAST1(Radioallergosorbent testing; 방사선 알레르기 흡착 시험법)에 양성으로 나타난 것을 기초로 한 면역요법(Immunotherapy)을 18개월 동안 시행했으나 성공적이지 않았다. 부신피질호르몬(Adrenocorticotropic Hormone, ACTH)자극 시험법은 이것이 본래 임상수의사에 의해 발생(Iatrogenic)된 것임을 예시하여준다. 피부 찰과물(Skin scrapings)을 검사한 결과 개 천공개선충(Sarcoptic mange)이라는 진단이 내려졌다. 이버맥틴(Ivermectin)치료효과와 피질스테로이드(Corticosteroid)의 투약중지에 대한 반응이 극적으로 나타났다. 10개월 추적기간(10-month follow-up period)동안에 이 질병은 재발되지 않았다. 이것은 비록 계절적인 알레르겐을 배제하기 위해 긴 기간동안 추적이 필요하였으나 개 천공개선충이 오히려 피질스테로이드요법에 대한 2차적인 것보다는 1차적인 질병이라는 것을 말해준다. 아토피의 진단을 위해 혈청학적 시험법의 이용이 논의되었다.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• v.10 no.2
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• pp.315-318
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• 2005

• Journal of Veterinary Clinics
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• v.26 no.5
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• pp.450-456
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• 2009

This study evaluated the efficacy of topical eprinomectin (0.5% pour-on solution, $Eprinex^{(R)}$, Merial) against Chorioptes texanus (C texanus) infestation in dairy cows reared in Korea. Dairy cows naturally infected with C texanus showed skin lesions as alopecia, erythema, excoriations and crusts on the tail head, the caudal folds, the back of udder and the rump area. Fourteen adult Holstein dairy cows with chorioptic mange at National Institute of Animal Science were selected on the basis of gross lesion and microscopic examination. Experimental dairy cows infected with chorioptic mange were treated with eprinomectin ($Eprinex^{(R)}$, Merial, France) at the rate of 1 ml /10 kg/BW. Evaluation of therapeutic effect is based on mite counts and gross lesion at weekly interval for 6 weeks. Milk yields were measured every day and calculate at the mean of milk yield at weekly interval. Mange mites absolutely removed after 1 week of topical eprinomectin against C. texanus infestation on dairy cows. The skin lesion of dairy cows infested with C. texanus recovered normal status after 6 weeks of eprinomectin treatment. After eprinomectin treatment, return to the normal level of milk yields in diary cows infested with C. texanus has indirectly indicated that C. texanus infestation have negative effect on productivity of dairy farm. However, it would be suggested that the investigation of actual condition and the effective control method of C. texanus infestation of dairy farms in Korea should be studied in the future.

• Journal of the korean veterinary medical association
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• v.17 no.6
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• pp.10-16
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• 1981

This work was intended to enhance diagnostic probability by the detection of eggs, not only mites, from the scrapings of skin lesions in three pigs infested with Sarcotes scahiei var suis, and the results were summarized as follows. 1. The amount of solve

• Parasites, Hosts and Diseases
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• v.58 no.3
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• pp.315-319
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• 2020

In November 2019 a 5-month-old mixed-breed rabbit presented to Chungbuk National University Veterinary Teaching Hospital, Cheongju-si, Chungbuk, Republic of Korea (Korea) with symptoms comprising pruritus, crusts on skin, poor appetite and reduced defecation. The rabbit was purchased 2 months prior from a pet shop located in a big market, and that the symptoms were first observed about 2 weeks prior to the hospital visit. Physical examination revealed that the patient had crust formation and alopecia on the nose together with lesions on the digits. A skin scraping test was performed using mineral oil and a high density of mites was observed by microscopy. Each mite showed a round, tortoiselike body with 4 comparatively short pairs of legs. The anus was located at the terminal unlike with suspected pathogen, Notoedres cati. Based on morphological characteristics, we identified the mite as Sarcoptes sp. Ivermectin was administered weekly by subcutaneous injection at a dosage of 0.4 mg/kg, and 4 weeks of follow-up study revealed the patient was fully recovered. And no more mites were detected from the case. This is the first case report of sarcoptic mange in a pet rabbit in Korea.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2006.05a
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• pp.129-129
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• 2006