• Journal of Plant Biotechnology
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• v.2 no.2
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• pp.109-113
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• 2000

Somatic hybrids were produced by electrofusion between embryogenic callus protoplasts of 'Syougun' mandarin and leaf protoplasts of grapefruit. Hybridity of the two plants was confirmed by leaf morphological characteristics and random amplified polymorphic DNA (RAPD) analysis. The cpDNA analysis using PCR-RFLP could not distinguish those of both parents. These plants showed normal growth and had chromosome number of 27. These unexpected triploid somatic hybrids might be derived from fused cells between diaploid protoplast of embryogenic calli and diploid protoplast of leaf, because polysomaty, a mixture of haploid cells and diploid cells was observed in the lactose medium-pretreated embryogenic calli of 'Syougun' by flow cytomehy analysis.

• Food Science and Preservation
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• v.5 no.1
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• pp.1-6
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• 1998

Physicochemical properties of early cultivar of Satsuma mandarin(Citrus unshiu Marc. var. miyagawa) according to harvesting year during 1990 to 1995 were investigated. Climatic conditions of every year affected the quality of citrus fruits. Compared to other harvesting years, flesh ratio and soluble solids were higher, but acid content was lower on citrus fruits produced in 1994, as for good climatic conditions during growth of fruits. Especially, acid content of fruits produced in 1993 was higher, compared to those of years, as for low mean temperature and high rainfalls. Fruit weight, peel thickness and soluble solids had a good linear correlation to fruits size, but acid content and flesh ratio had not correlation derived from difference of individual fruits. From the climatic data, the prediction of fruit quality would be possible in some degree before harvest. It seemed recommend to ably the flexibe method by harvesting year for the quality stanardization of citrus fruit.

• Proceedings of the Korean Society for Language and Information Conference
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• 2007.11a
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• pp.532-541
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• 2007

This paper investigates children's comprehension and production of demonstrative pronouns (DPs), 'zhege' (this) and 'nage' (that), in Mandarin Chinese. Subjects are children of ages three, four, five and six. Based on the results of the present experiment, children's developmental stages and the corresponding age grading are provided. Also, the present study incorporates a physical clue into the experiment. The result suggests that in the acquisition of deixis children rely highly on physical context to work out the meaning distinction. In addition, Piaget's egocentrism hypothesis and H. Clark's marking hypothesis are examined in the study. The result seems to support the egocentrism hypothesis. Subjects under the age of six do fail to shift the deictic center when they and the experimenter have a different perspective. As for the marking hypothesis, the study seems to challenge the hypothesis. The result shows that children actually performed better on the marked term 'zhege' than the unmarked member 'nage'.

• Korean journal of food and cookery science
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• v.6 no.1
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• pp.9-12
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• 1990

This study was carried out in order to investigate the physicochemical properties of isolated pectic substances from Citron, Satsuma mandarin, Marumero, and Natsudaidai. Among varieties, Citron had the highest value of isnoluble pectin and Marumero had the hightest ratio of soluble pection. The total pectin contents isolated by 2% Sodium hexameta-phosphate(SHMP) varied in the range of 1.85% ~ 2.63%. Anhydrogalacturonic acid(AUA) contents were 78.64%~89.14% and were lower than commercial pectin. Isolated pectin contained above 7% methoxyl content and above 50% esterification. The apparent molecular weight of commercial pectin and isolated pectin were 72,000 and 42,000~46,000 respectively. Esterification and ester methoxyl content were the lowest and apparent molecular weight was the highest in Satsuma mandarin. Citron's values were a little higher in esterification and ester methoxyl content and lower in apparent molecular weight than the others.

• The Plant Pathology Journal
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• v.31 no.4
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• pp.421-427
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• 2015

Citrus scab disease is one of the destructive diseases that reduce the value of fruit for the fresh market. We analyzed the process of symptom development after infection with scab pathogen $Elsino{\ddot{e}}$ fawcettii in the susceptible satsuma mandarin leaves to observe the structural modification against pathogen. The cuticle and epidermal cells along with 3-5 layers of mesophyll tissue were degraded 1-2 days post inoculation. Surrounding peripheral cells of degraded tissues grew rapidly and then enveloped the necrotic area along with the growing conidia. Cross sections through the lesion revealed hyphal colonization in epidermis and mesophyll tissues. In response to the pathogen colonization, host cell walls were lignified, inner cells were rapidly compartmentalized and a semi-circular boundary was formed that separated the infected region from the non-infected region, and finally prevented the intercellular pathogen spread.

• Proceedings of the KSPS conference
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• 2003.05a
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• pp.43-47
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• 2003

This paper is phonetic study of $F_{0}$ range and boundary tone in Mandarin Chinese. The production data from 6 Chinese speakers show that there are declination, pitch resetting and tonal variation of boundary tone. In declarative sentence, $F_{0}$ declines gradually over the utterance but mid-sentence boundary prevents $F_{0}$ of following syllable from declining because of pitch resetting. $F_{0}$ range of syllable is expanded before the mid- and final sentence boundaries. In interrogative one, $F_{0}$ ascends gradually over the utterance and mid-sentence boundary makes $F_{0}$ of following syllable rise more. $F_{0}$ range of sentence final syllable is expanded and $F_{0}$ contour shows rising curve.

• YAKHAK HOEJI
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• v.47 no.1
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• pp.25-30
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• 2003

This study is performed to investigate the effects of citrus essential oils on melanin production in B16 melanoma cells. Five kinds of citrus essential oils (Bergamot, Grapefruit, Lemon, Mandarin, Petigrain) did not have any influence on DPPH radical scavenger activity, cell growth and cytotoxicity in B16 melanoma cells. Both mandarin and petigrain essential oils dose-dependently inhibited purified tyrosinase activity, but bergamot did not. In 1$\mu$M MSH-stimulated B16 melanoma cells, all of 5 citrus essential oils inhibited melanin production in a dose dependent manner. From the above results, it is possible that citrus essential oils may be developed to be an anti-melanogenesis agent on the basis of their inhibitory effect on MSH-induced melanin production.

• Proceedings of the Korean Society for Language and Information Conference
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• 1995.02a
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• pp.91-96
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• 1995

• Proceedings of the Korean Society for Language and Information Conference
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• 1995.02a
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• pp.231-236
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• 1995

• Proceedings of the Korean Society for Language and Information Conference
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• 1995.02a
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• pp.237-242
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• 1995