• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.59-63
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• 2021

To study for germination rate of apple artificial seeds encapsulated with 2% alginic acids, the rates of germination for artificial seeds of various embryos with partial elimination of cotyledon measured by adding 80 mg/L gibberellic acid or/and nutritional media. The germination rate of artificial seeds produced embryo with 3/4 cotyledon (3/4 CE), embryo with 1/2 cotyledon (1/2 CE), embryo with 1/4 cotyledon (1/4 CE), and embryo without cotyledon (NCE) were gradually decreased with compare to normal embryo, but it's considerably recovered on the alginic acid containing MS solution or/and 80 mg/L GA3. In special, the low frequency (55.81%) of germination for artificial seeds of 1/2 cotyledon embryo with alginic acid was increased by treatment with combination of alginic acid, 3% sucrose, and 80 mg/L gibberellic acid (98.74%). In case of embryo without cotyledon, the low frequency of embryo without cotyledon (24.44%) in alginic acid almost recovered to 82.45% in alginic acid with MS solution and 80 mg/L gibberellic acid. The results shown that the germination of embryo was decreased by the removal of cotyledon, and demonstrated that the low rate of germination could be considerably increased by adding GA3 (80 mg/L) or/and nutrition.

• Journal of the Korean Society of Floral Art and Design
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• no.37
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• pp.157-166
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• 2017

To select fruit trees for urban garden with Elaeagnus umbellata var. coreana, Malus domestica 'Alps Otome', Prunus mume, Ribes nigrum, Rubus fructicosus, and Vaccinium corymbosum 'Reka', growth characteristics of these 6 species were compared and effect of trickle irrigation and shading on the growth of these fruit trees were investigated that these treatments were carried out how much these trees are endurable exposed to improper conditions. After passing the winter, E. umbellata var. coreana, M. domestica 'Alps Otome', P. mume, and V. corymbosum 'Reka' showed the highest survival rate, in contrast to R. nigrum with the lowest of 56.0%. In 9 months after planting, the hight growth rate of 62.3% was shown in P. mume but that of E. umbellata var. coreana was stagnant during experimental period. Flowering rate over 80.0% were shown in E. umbellata var. coreana, M. domestica 'Alps Otome', R. fructicosus, and V. corymbosum 'Reka'. On the other hand, R. nigrum has not ever flowering branches. Other species except E. umbellata var. coreana and R. nigrum have about 10 days in flowering duration. After flowering, fruition rate has shown significant difference among 6 species. As a result of both treatment of trickle irrigation and shading, leaf area and leaf weight of E. umbellata var. coreana, M. domestica 'Alps Otome' significantly inceased with treatments that have not affected other species. Based on these result, it is possible to deploy suitable fruit trees to specific garden condition after selecting various fruit trees.

• Journal of Plant Biotechnology
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• v.50
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• pp.176-182
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• 2023

Plant regeneration protocols for adventitious shoot organogenesis from apple (Malus domestica 'Fuji') leaf explants were developed in the present study. The effects of dark incubation periods in the early stages of culture, pre-treatment methods, the number of explants per culture container, the type of culture containers, and the orientation of the explants on culture media were evaluated to determine the optimal shoot regeneration conditions for 'Fuji' apple leaf explants. Light incubation of explants produced minimal response. However, dark incubation of explants for 4 weeks during the initial culture period enhanced shoot regeneration frequency. Comparing the number of explants per container, a higher percentage of shoot regeneration was obtained with nine explants per container compared with four explants per container. Pre-treatment, before culture, by dipping explants in a liquid regeneration medium containing 40 g/L of sorbitol for 2 hours produced the highest shoot formation rate, and the time of shoot formation was accelerated. The percentage of shoot regeneration and number of shoots per regenerating explant reached a maximum of 87.5% and 4.7, respectively. The regenerated shoots were elongated and rooted on a rooting medium of 1/4 MS with 0.2 mg/L IBA. The plantlets were successfully acclimatized, and the regenerated plants produced normal phenotypes.

• Applied Biological Chemistry
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• v.20 no.1
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• pp.66-80
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• 1977

This research was carried as a part of the basic study, in which the aptitude of theKorean oak varieties as barrels for aging apple fine spirits was investigated, and thefollowing results were obtained. 1. Following was the result of the chemical analysis of the fruits which are now mass-produced and can be used as a substitute for raw materials for wine production. Apple (Malus pumila Miller var. domestica Schneider) : Total sugar. total acid, volatile acid and pectin of Jonathan (Hong-og) were 13.95%, 0.46%, 0.012%, 0.20% respectively. Total sugar, total acid, volatile acid and pectin of Ralls (Koog-kwang) were 13.35%, 0.43%, 0.011%, 0.45% respectively. 2. Because of low yield of apple juice due to cellulose, pectin, hemicellulose which are present besides sugars, acids in apples, the apple juice were treated with xylanase of Aspergillus niger SUAFM-430, cellulase and pectinase of Aspergillus niger SUAFM-6. This treatment increased the yield of apple juice. And the apple juice was sterilized by adding potassium metabisulfite $(K_2S_20_5)$ and Saccharomyces cerevisae var. ellipsoideus Rasse Johannisberg II (SUAFM-1018) as a cultivation yeast, which has a strong fermentation power was used to ferment. The yield of apple wine based on raw material was 86-87%. The amount of ethanol, extract and methanol obtained from Jonathan and Ralls were 13.5%, 5.4%, 0.04-0.05% respectively. 3. Wines were distilled for two times by the pot still method to make fine spirits. The yield of fine spirits from apple wine mash was 86.6%, and the pH of fine spirits from Jonathan and Ralls were 4.1, 4.2 respectively. 4. The oak chips made of inner part or outer part of 24 Korean oak varieties were used to select the barrel for aging fine spirits. Two oak chips (one oak chip: $1{\times}1{\times}5cm$) of the inner part or of the outer part of each oak variety were dipped into 300 ml of fine spirits, which was bottled in 640ml beer bottle, and followed aging. The colors, flavors and tastes of the fine spirits were checked during 6 months. A. As a criterion for the first screening of oak barrels for aging fine spirits, the rate five of color extraction was determined. The oak chips showed good results in their order as follows and the best 5 varieties were selected. Gal-cham: Quercus aliena Blume (Inner part), Gul-cham: Quercus variabilis Blume (Outer part), Gal-chain: Quercus aliena Blume (Outer part), Jol-cham: Quercus serrata Thumb (Inner and Outer part). Sin-gal-cham: Quercus mongolica Fisher (Outer and Inner part) Sang-su-ri: Quercus acutissima Carruthers (Outer and Inner part) B. To find out the influence of aging temperature on aging, apple fine spirits were aged by dipping each oak chip at room temperature $(24-25^{\circ}C)$) and $45^{\circ}C$. Aging at $45^{\circ}C$ gave the best result followed aging at $30^{\circ}C$ and then at room temperature. C. Apple fine spirits was aged for six months by dipping oak chips in Erlenmeyer flasks and was irradiated with U.V light. The U.V irradiation enhanced the aging effect by nearly two times, compared with the aging without U.V irradiation. D. In aging apple fine spirits by dipping two oak chips, it was observed that the extent of the extraction of most components of oak chips were strongly dependent upon the pH of fine spirits. E. Oak chips of five selected oak varieties and a Limousin white oak from France as a control were used. Each apple fine spitits was dipped by two oak chips, and was aged at room temperature $(24-25^{\circ}C)$, $30^{\circ}C$, $45^{\circ}C$, and with the U.V irradiation at room temperature shaking every week. After six months of aging, the panel test of these aged fine spirits (Young Brandy) showed the following result. Young brandy of apples aged at $45^{\circ}C$ by dipping oak chips of Gal-chain was almost as the fine spirits which were aged at room temperature by dipping Limousin white oak chips from France. Young brandy of with U.V. irradiation at room temperature which were aged by dipping oak chips of Gal-chain was a little worse than that from the fine spirits aged at room temperature by dipping Limousin white oak chips from France. And so, Korean oak varieties are thought to be able to be used for aging every apple fine spirit which was here investigated.

• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.3
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• pp.130-144
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• 2013

In this study, we examined the effect of high temperature of urban area on the physiological response of apple tree including the photosynthesis, shoot growth, and fruit quality of 'Fuji'/M.9 apple trees planted at Daegu urban area (DUA) and Gunwi rural area (GRA) for 2 years (2009-2010). During the apple growing season (April-October), the average air temperature of DUA was about $3.0^{\circ}C$ higher than that of GRA and the total rainfall of DUA was 130 mm more than that of GRA. During fruit enlargement stage (June-August), the number of days that recorded daily mean temperature of over $30^{\circ}C$ were ten on DUA in 2010, but there was no day when such temperature was experienced in 2009. Average air temperature of DUA during the maturation stage (September-October) was $19.8^{\circ}C$, which was $4.0^{\circ}C$ higher than that of GRA. The higher temperature of over $30^{\circ}C$ during fruit enlargement stage decreased the photosynthetic rate, shoot growth, fruit weight, and soluble solid content of 'Fuji'/M.9 apple tree. The moderate temperature of about $20^{\circ}C$ during maturation stage increased the photosynthetic rate and soluble solid content of 'Fuji'/M.9 apple tree, but decreased fruit red color. In regional comparison with GRA, photosynthetic rate of DUA was changed from lower before rainy season to higher after rainy season. Fruit weight was higher in DUA than that of GRA. However, fruit weight between DUA and GRA did not show the difference when accumulated days that recorded daily maximum temperature over $35^{\circ}C$ of DUA was increased. Compared to the GRA, soluble solid content of DUA was higher, but fruit red color of DUA was less. These results indicate that the poor red coloring is the most problematic in 'Fuji'/M.9 apple tree by global warming and urbanization.

• Korean Journal of Environment and Ecology
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• v.34 no.1
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• pp.85-92
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• 2020

The purpose of this study is to provide base information that can be utilized in surveying the status of landscape management within apartment complexes and grasping the conditions of the soil for planting plants, as a way to improve the quality of green spaces by repairing poorly vegetated spaces within apartment complexes and remodeling them with diverse species of plants. Considering that trees and shrubs of equal sizes are simultaneously planted upon the completion of apartment complexes, they were divided based on their completion year at the interval of 5 years and differences in the growth of trees and the components of soil over time were analyzed. Out of the species of trees planted in all the 9 apartment complexes surveyed in this study, the growth of 4 species of needleleaf trees and 4 species of broadleaf trees were surveyed. Juniperus chinensis 'Kaizuka' and Pinus densiflora Siebold & Zucc. out of the needleleaf trees and Malus floribunda Siebold ex Van Houtte out of the broadleaf trees showed the highest growth rate when over 5 years passed after planting and their growth rate decreased when over 10 years passed. Platycladus orientalis and Acer palmatum Thunb. in the apartment complexes that were built over 10 years ago showed the highest growth rate, which indicates that the species require a relatively long period of time for growth. The hardness of the soil at the areas where trees were planted but their soil surface was bare was analyzed. When over 5 years passed after the completion of apartment complexes, over 20 mm of the soil was found to be stamped. The physicochemical properties of soil were also surveyed and the pH level was found to have been continuously high ever since the completion. The organic content in the surveyed soil was about 1/3 of the content in fertile soil, which means that additional fertilization is required. These results indicate that the stamped soil and the health of soil can be restored, when replanting plants in bare areas, by adding plans to improve soil, such as designing drain ways around the planted areas, transporting soil for the 50 cm depth of the ground and mixing organic matters such as chaff, and simply by planting groundcover plants in the lower part of tress and shrubs.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.1
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• pp.10-19
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• 2000

Due to the urbanization, concentrated population, and limited land exploitation in the modern society, the environment surrounding that we live in is getting polluted more and more, and it has become hard even to let urban children experience the nature. This research was conducted to help people recognize the importance of our natural resources through the environmental education of elementary school and to use school's practical open-space for the Indigenous Plants education. The results of this study are as follows : First, the status of a plant utilization in our institutional education : There were 362 species totally of 124 species of Trees, 156 species of Herbs, 63 species of Crops, and 19 species of Hydrophytes which appear in the elementary school text book. Of all, the most frequently appearing species of tree were the Malus pumila var. dulcissima, Pinus densijlora, Citrus unshiu, Diospyros kaki. Second, the effect of plant education using the land around schools : The result of research on the open-space of the 19 elementary schools located in Mapo-gu showed that most of the species planted are the Juniperus chinensisrose, Hibiscus syriacus. Pelargonium inquinans in the order of size, and the plants appearing in text book were grown in the botanical garden organized in 7 schools. Especially most of the Indigenous Plants were being planted in botanical garden, and Pinus densijlora, Abeliophyllum distichum, Polygonatum var. plurijlorum, Liriope platyphylla and so on. Last, the result of this research on recognition of Environment, Planting education and Indigenous plants : It showed that educational necessity of students and teachers about environment and Indigenous Plants was more than 80%. The management of botanical garden was conducted by some teachers and managers. The results of this study suggested that we needed the reconstruction of curriculum, the efficient application of plant education for effectiveness of using school environment and monitoring continually and construction information sources for the better environment education in the elementary schools.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.155-165
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• 2015

Well-feathered (over 10 feathers) 'Fuji'/M.9 apple trees were planted at $3.0{\times}1.0m$ and trained to slender spindle with 2.5 m height or to tall spindle with 3.5 m height, and the vegetative growth, productivity, and fruit quality of two training systems were compared for 8 years. The canopy volume of the tall spindle trees surpassed that of the slender spindle trees 4 years after planting and was 25% larger than that of the slender spindle trees 5 years after planting. The accumulated yield over 8 years for the tall spindle system was 14% higher than that of the slender spindle system. Alternate bearing and incidence of marssonina blotch were observed in both treatments after 5 years of planting. There was often vegetative imbalance in the trees however, the degree of yield loss and vegetative imbalance of the tall spindle trees was lower than those of the slender spindle trees. Soluble solid content and fruit red color of the tall spindle trees were higher than that of the slender spindle trees in 5 year after planting, resulting from increased light penetration in the canopy due to even distribution of lateral branches and from fruit bearing in different height locations of the trees. In conclusion, increasing the tree height to about 3.5 m using slender spindle 'Fuji'/M.9 apple trees planted with over 333 trees per 10a led to better light penetration, yield and fruit quality compared to a conventional wide training system with the slender spindle.

• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.1
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• pp.1-8
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• 2013

This study was conducted to compare the local climate conditions and growth periods for the apple (Malus domestica 'Borkh') orchards at different altitudes (330, 500, and 670 m) in Jangsu-Gun, Korea. Observation data for the growth period show that the monthly mean air temperatures at the 'Hongro' apple orchard sites decrease with height at the rate of 1.0 to $3.0^{\circ}C$/100 m. The monthly minimum temperatures in April (blooming period for 'Hongro' apple) were 4.3, 2.9, and $0.4^{\circ}C$ at 330, 500, and 670 m, respectively. The monthly mean temperatures in September (i.e., the coloration and maturation period) were 20.6, 18.7, and $14.5^{\circ}C$, respectively. The annual precipitation range varied from 1,234 to 1,439 mm, which tended to increase with height. The heavy rainfall occurred in summer (June to August) and amounted to 827-933 mm. No significant differences in the duration of sunshine were observed amongst the orchards at three different altitudes. The earliest bud break was observed at the 330 m altitude (18 March 2009), which was 4 and 11 days earlier in comparison to those at 500 and 670 m, respectively. The time of full bloom at 330 m was 12 days ahead of that at 670 m. The optimal maturation of fruit (based on skin redness > 80%) was observed between 7 and 10 September at 330 m, 15 and 18 September at 500 m, and 21 and 23 September at 670 m.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.178-183
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• 2014

This study aimed to evaluate the fruit quality characteristics and incidence of flesh browning in response to air storage at $0{\pm}1^{\circ}C$ a fter controlled atmosphere s torage (CA condition: $O_2$ $2.5{\pm}0.5$%, $CO_2$ $1.5{\pm}0.5$%) at $0{\pm}1^{\circ}C$ in 'Fuji' apples (Malus domestica Borkh.). The storage system was performed as followed: air storage for one month, CA storage 4 months + air storage 3 months ( CA 4M + A ir 3M), CA storage 5 m onths + air storage 2 months ( CA 5M + Air 2M) a nd C A storage 6 m onths + air storage 1months (CA 6M + Air 1M), while the control fruits were stored at CA storage for 8 months right after harvest. The incidence of flesh browning ranged from 17.1% to 30.2% during CA storage but not detected under the treatments of CA 4M + Air 3M and CA 5M + Air 2M. The respiration rate was not affected by storage treatments for 6M while the respiration rate was lower in the treatments of CA 4M + Air 3M and CA 5M + Air 2M than the other storage treatments after 7 months. Ethylene production and internal ethylene concentration were lowest in rapid CA storage and increased with a decreasing CA storage duration. Therefore, the results indicate that CA 5M + Air 2M storage treatment should be recommended to maintain the fruit quality and reduce the risk development of flesh browning rather than typical CA storage in 'Fuji' apples.