• Journal of Animal Science and Technology
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• 제44권5호
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• pp.531-540
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• 2002

한우 수송아지에 대한 육성기 항병력 증진물질 투여 효과를 구명하기 위하여 75일령에 이유된 한우수송아지 30두를 대상으로 사료내 항병력 증진물질 첨가 종류에 따라 6개 처리(T1: 관행사육, T2: 황토 2%첨가, T3: Illite 2% 첨가, T4: Oligosacharides 0.04% 첨가, T5: Charcoal powder 2% 첨가, T6: Chromium picolinate 0.1% 첨가)를 두어 3개월령부터 7개월령까지 120일간 사양시험을 실시한 결과, 처리구별 일당 증체량은 T3, T6, T4, T5, T2 및 T1이 각각 0.92, 0.90, 0.90, 0.88, 0.83 및 0.82kg으로 Illite 및 크롬은 이유직후 송아지의 성장발육에 영향을 미쳤으나 가공되지 않은 황토급여는 성장발육에 뚜렷한 효과가 없었다. 육성기 120일간 1일 평균 농후사료 섭취량 및 체중대비 섭취비율은 각각 3.91${\sim}$4.15kg, 평균 4.03kg 및 3.1${\sim}$3.31%, 평균 3.21%였고, 1kg 증체에 소요된 TDN 량은 3.20${\sim}$3.57kg, 평균 3.35kg으로 T5, T3, T6, T4, T2 및 T1 순으로 우수하였다. 송아지의 혈청 내 IgG 농도는 처리구별로 10.2${\sim}$11.6mg/$m\ell$ 내에서 변화하여 정상 축에서 볼 수 있는 범위 내에 있었지만, T3 및 T5는 개시시에 비해 각각 6.9 및 2.8% 증가하였으며, 질병발생은 처리구별로 뚜렷한 차이가 없었다. 이상과 같은 결과들로 볼 때, 점토광물인 Illite는 다른 항병력 증진물질과 마찬가지로 어린 송아지의 발육 및 사료이용성을 증진시키지만 가공되지 않은 황토의 급여는 발육 및 사료이용성에 뚜렷한 차이가 없는 것으로 나타났다.

• 한국동물생명공학회지
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• 제34권2호
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• pp.111-116
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• 2019

This study was conducted to analyze the specific genes associated with sex-determination in Korean native cow. The highly organized spermatogenesis requires accurate spatial and temporal regulation of gene expression, which is governed by transcriptional, post-transcriptional, and epigenetic processes. Recently, farmers have been interested in determining the sexual identity of the calves in their farm. We analyzed the sperm of Korean native and Holstein cows, which were supplied from Hanwoo Improvement Center. We evaluated sperm motility and expression of sperm-specific genes after treating semen with both male- and female reagents. Sperm motility in Korean native cows decreased by approximately 10% in the first 30 minutes after treatment with sex-determination reagent. However, sperm motility of Holstein cows decreased to 60-70% after 15 minutes and to 20-30% after 30 minutes. We selected six specific genes expressing in the spermatozoa to analysis the gene expression level. The Real-time PCR results suggest that the selected genes (Gimap4, Tmeff1, Rac2, Abi2, Rac1, and Clu) were highly expressed in the group treated with the male reagent compared to the group treated the female reagent and to the untreated-group (control). In the present study, we suggest that the selected genes play a pivotal role in sex-determination.

• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.45-49
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• 1992

Bovine drumstick of neutrophil leucocytes was studied on the quantitative and morphological characteristics and was evaluated as a diagnostic measure for bovine freemartin in newborn calves. Nuclear area of neutrophil (A, ${\mu}m^2$) and drumstick area (B, ${\mu}m^2$) were significantly correlated with average diameter of drumstick (ADD, ${\mu}m$) and following regression equations were obtained : $A=45({\pm}3)$ ADD-8, r = 0.74, $s.e.{\pm}0.6$, p < 0.01, $B=1.72({\pm}0.05)$ ADD-0.98, r = 0.93, $s.e.{\pm}0.1$, p < 0.01 Eight female siblings of heterosexual multiplets were diagnosed as freemartin from the results of chromosome analysis. Heterosexual multiplets had a very low frequency of drumstick in the nucleus of neutrophils irrespective of genetic sex. Diameters of drumstick fund in freemartin and male cotwin did not differ from those of normal cows. Examinations of drumstick in 800 neutrophils for both female and male siblings are concluded to be the best way to aid the detection of freemartinism of heterosexual twins at early life.

• Asian-Australasian Journal of Animal Sciences
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• 제5권3호
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• pp.533-539
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• 1992

Sixteen crossbred (Sahiwal $\times$ Holstein) male rumen fistulated calves of 18 to 24 months of age were randomly divided into four groups of four animal, each. Animals in all the groups were fed wheat straw ad lib as basal roughage. However, the animals in group I were fed concentrate mixture at maintenance level, whereas, the animals in groups II, III and IV had free access to existing, modified (A) and modified (B) urea molasses mineral block licks respectively. Daily wheat straw intake (kg) was significantly (p<0.01) higher in groups II ($4.20{\pm}0.13$), III ($4.07{\pm}0.16$) and IV ($4.22{\pm}0.20$) as compared to group I ($3.21{\pm}0.14$). Total N and TCA precistrained rumen liquor) was significantly higher in groups II ($22.36{\pm}0.25$), III ($21.63{\pm}0.25$) and IV ($21.77{\pm}0.55$) as compared to group I ($18.31{\pm}0.41$). Bacterial production rate (g/day and g/kg digestible organic matter intake) were non-significantly different amongst groups I ($214.4{\pm}13.28;\;85.38{\pm}3.69$); II ($198.7{\pm}5.70;\;86.17{\pm}3.53$); III ($214.4{\pm}8.19;\;96.15{\pm}2.16$) and IV ($218.2{\pm}10.62;\;94.44{\pm}5.52$). Similarly, percent efficiency of N incorporation into bacterial protein was not found significantly different amongst groups I, II, III and IV. These studies indicate that when concentrate mixture (upto maintenance level) in the diet of ruminants was replaced with UMMB licks, various N fraction in SRL and efficiency of bacterial production rates in the rumen were not affected.

• 대한한의학회지
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• 제42권2호
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• pp.107-119
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• 2021

Objectives: This study was aimed to report changes in clinical symptoms and signs after treatment with Korean medicine in patients who suffered from calf edema due to deep vein thrombosis (DVT). Methods: A 46-year-old male patient, who discharged home after receiving standardized treatment for acute DVT in the right leg, suffered pain and heat sense due to edema of the affected calf. Oral administration of herbal medicine (Gyejibongnyeong-hwan 4 g, twice daily) and acupuncture therapy were given to him for 6 weeks. The change in chief complaints, Villalta score, the right and left side difference of the circumference and the skin surface temperature of both calves, and blood level of D-dimer were observed before and after treatment. Results: The patient's chief complaints began to significantly improve from 2 weeks after treatment, and disappeared completely within 4 weeks. At the end of the treatment, a substantial decrease in the difference between the right and left calves in the circumference and skin surface temperature was observed. This effect was maintained even at the time of follow-up 3 months after the end of treatment, and the patient completely recovered indoor and outdoor life to the level before onset. Conclusions: This case suggests that Korean medicine treatment, including Gyejibongnyeong-hwan administration and acupuncture therapy, can be a viable option to improve edema and related clinical problems in the affected limbs due to DVT.

• 한국융합학회논문지
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• 제13권5호
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• pp.307-312
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• 2022

본 연구에서는 종아리 근육에 기능성 의류를 착용한 것이 종아리의 근기능에 어떤 영향을 미치는지 규명하고 기능성 의류가 종아리 근기능 증가에 활용가치가 있는지 검증하고자 하는데 그 목적이 있다. 연구의 대상은 하지에 이상이 없는 B 대학교의 남학생 21명을 대상으로 측정 하였다. 측정방법은 등속성근기능측정기인 CSMI사의 Humac Norm을 이용하여 오른쪽, 왼쪽 종아리에 기능성 의류 착용 전, 후 각속도 30°/sec, 120°/sec로 측정하였다. 피실험자들이 기능성 의류를 착용했을 때와 착용하지 않았을 때를 측정 해본 결과 발목 배측굴곡(Dorsi flexion)에서는 기능성 의류를 착용했을 때와 착용하지 않았을 때 유의한 차이가 나타나지 않았으나, 발목 저측굴곡(Plantar flexion)에서는 기능성 의류를 착용하였을 때 근력, 근파워, 근지구력이 유의하게 증가하는 것으로 나타났다. 따라서 종아리의 기능성 의류 착용이 운동기능 향상에 긍정적 효과가 있으며, 향후 연구에서는 대회를 앞두고 훈련을 하는 선수에게 기능성 의류를 착용한 인원과 착용하지 않은 인원의 근기능 향상정도를 비교해보는 연구를 제시하는 바이다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.325-329
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• 2004

Ten male buffalo calves (aged 6-8 months, average body wt. $88.5{\pm}0.5kg$) were divided into two groups of five animals in each. All the animals were fed on urea-ammoniated wheat straw (4% urea, 50% moisture) along with concentrate mixture (50:50 on DM basis). In addition animals in group II were given sodium sulphate to see the effect of sulphur on the utilization of nitrogen added through urea-ammoniation. This feeding practice continued for a period of 120 d, during which fortnightly body weights were taken to assess their growth rate. A metabolism trial was conducted after 90 days of feeding to know the digestibility of nutrients and their balance. Results revealed no significant difference in the intake of DM and other nutrients in two groups. The digestibility of DM, OM, EE, NDF, ADF and cellulose was alike in animals fed ammoniated straw and ammoniated straw+sodium sulphate supplemented group, whereas the digestibilities of CP and hemicellulose was significantly (p<0.01) more in group I and II respectively. There was no significant difference in intake of nitrogen, calcium and phosphorus in 2 groups. Similarly, the balance of these 3 nutrients was positive and statistically alike in two groups. Intake and excretion of sulphur through faeces and urine was significantly (p<0.01) more in group II than in group I. Inspite of higher excretion of sulphur through faeces and urine in group II, the sulphur balance was significantly (p<0.05) more in group II than in group I, probably due to significantly (p<0.01) higher intake of sulphur in this group. There was no significant difference in total body weight gain or average daily gain between two groups, indicating that addition of sodium sulphate did not have any positive effect on these parameters. Similarly the intake of DM, DCP and TDN were also alike in two groups. The DCP and TDN values of the two diets were 8.0, 60.4 and 6.8, 56.6% respectively. Feeding cost/unit gain was alike in both the groups.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1341-1347
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• 2002

An experiment was conducted to determine effects of oral administration of $Diakur^{TM}$ (an additive of glucose and electrolytes for young calves) on growth performance and some physiological responses in male broilers reared in a high temperature. A 2 by 3 factorial arrangement test of 2 temperatures (24 and $36^{\circ}C$) and 3 levels of oral administration of the glucose and electrolytes additive, $Diakur^{TM}$, (0, 150 and 300 mg/day/100 gBW) were applied in the experiment. Male broiler chicks (2 weeks of age) were assigned to six groups and received dietary and temperature treatments for 7 days. The additive of glucose and electrolytes was suspended with water and intubated into crop twice a day (08:00 and 17:00). Oral administration of the additive prevented decreases in food intake and growth rates in broilers due to exposure of the hot environment. Oral administration of the additive also improved a lowered electrolyte ($Na^+$ + $K^+$ - $Cl^-$) balance in plasma, low mitogenic response of blood mononuclear cell and an increase in glucose concentration due to exposure to the high environmental temperature. Oral administration of the additive increased rectal temperature regardless of environmental temperatures. On the other hand, blood pH, $pCO_2$ and $HCO_3$ - concentration, and plasma creatine kinase activity were not affected by the oral administration. The results suggested that oral administration of the glucose and electrolytes additive, $Diakur^{TM}$ during heat stress did not only prevent decrease in growth performance, but also normalized some physiological and immunological responses in male broilers.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1188-1195
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• 2001

A tetraparental chimeric bull was successfully produced by aggregating bovine IVF embryos of F1 (female Holstein${\times}$male Japanese Black) and F1(female Japanese Brown${\times}$male Limousin) and culturing in vitro without the zona pellucida at Yamaguchi Research Station in Japan. In the microsatellite genotyping, 12% (28/228) microsatellite primer sets ware potentially useful for this parentage analysis in the chimeric bull, 78.6% (22/28) of microsatellite present in the chimeric bull were uniquely contributed from the Japanese Black and 21.4% (6/28) from Limousin. This chimeric bull semen was used in producing IVF embryos. The chromosome preparations were made from peripheral lymphocytes. Based on chromosome analysis the Chimera had apparently normal chromosomes (29 acrocentric pairs, one large sub metacentric X chromosome and one small sub metacentric Y chromosome). The proportion of acrosome reacted spermatozoa after 1 h of incubation was higher (p<0.01) with the Chimera than with the Holstein and in Japanese Brown bulls. But did not differ from Japanese Black and Limousin bull sperm. Fertilization rates observed after 5 h of sperm-oocyte incubation with Chimera sperm were higher (p<0.05) than with Japanese Brown and (p<0.01) than with Holstein sperm, but did not differ from Japanese Black and Limousin sperm. The cleavage rates of IVF oocytes inseminated with Chimera sperm were also higher (p<0.001) compared with Holstein, (p<0.01) Japanese Brown and (p<0.05) Limousin, but did not differ from Japanese Black sperm. The blastocyst rates of IVM oocytes inseminated with sperm were higher (p<0.05) than in Limousin, Japanese Brown and Holstein, but did not differ from Japanese Black. Chimeric cattles were produced by aggregation of parthenogenetic (Japanese Brown) and in vitro fertilized (Holstein) bovine embryos at the Yamaguchi Research Station in Japan and by aggregation of parthenogenetic (Red Angus) and in vitro fertilized (Holstein) embryos at the St. Gabriel Research Station in Louisiana. The aggregation rate of the reconstructed demi-embryos cultured in vitro without agar embedding was significantly lower than with agar embedding. The aggregation was also lower when the aggregation resulted from a whole parthenogenetic and IVF-derieved embryos cultured without agar than when cultured with agar. The development rate to blastocysts, however, was not different among the treatment. To verify parthenogenetic and the cells derieved from the male IVF embryos in blastocyst formation, 51 embryos were karyotyped, resulting in 27 embryos having both XX and XY chromosome plates in the same sample, 14 embryos with XY and 10 embryos with XX. The viability and the percentage of zonafree chimeric embryos at 24 h following cryopreservation in EG plus T with 10% PVP were significantly greater than those cryopreserved without PVP. Pregnancies were diagnosed in both stations after the transfer of chimeric blastocysts. Twin male and single chimeric calves were delivered at the Yamaguchi station, with each having both XX and XY chromosomes detected. Three pregnancies resulted from the transfer of 40 chimeric embryos at the Louisiana station. Two pregnancies were Jost prior to 4 months and one phenotypically chimeric viable male born.

• Asian-Australasian Journal of Animal Sciences
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• 제3권4호
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• pp.287-292
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• 1990

1.86 million (Indigenous river, swamp, and swamp X river type) buffaloes are distributed mainly in the plain land, sugarcane belt and coastal area of Bangladesh and are raised by the small farm holders. Buffaloes per house-hold ranges from 5.79 to 2.12. Height at wither is $123{\pm}3.09$ and $112.5{\pm}2.15cm$ for buffaloes of central and eastern region respectively. Growth rate of buffalo calves ranges from 360 to 340 g/day. Late maturity ($1411.58{\pm}43.01d$) along with long life span facilitates farmers to use buffaloes longer period. Average daily milk yield is $2.32{\pm}0.63L$ with average lactation yield of $730{\pm}90l$ for $328{\pm}28.76d$. Both male and female individuals are used for draught purpose. A pair of buffalo can prepared $0.23{\pm}0.06ha$ of land daily and can work for $6.1{\pm}0.78hr$.