• Korean Journal of Microbiology
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• v.55 no.2
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• pp.103-111
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• 2019

DNA methylation is involved in diverse processes in bacteria, including maintenance of genome integrity and regulation of gene expression. CcrM, the DNA methyltransferase conserved in Alphaproteobacterial species, carries out $N^6$-adenine or $N^4$-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Celeribacter marinus IMCC12053 from the Alphaproteobacterial group was isolated from a marine environment. Single molecule real-time sequencing method (SMRT) was used to detect the methylation patterns of C. marinus IMCC12053. Gibbs motif sampler program was used to observe the conversion of adenosine of 5'-GANTC-3' to $N^6$-methyladenosine and conversion of $N^4$-cytosine of 5'-GpC-3' to $N^4$-methylcytosine. Exocyclic DNA methyltransferase from the genome of strain IMCC12053 was chosen using phylogenetic analysis and $N^4$-cytosine methyltransferase was cloned. IPTG inducer was used to confirm the methylation activity of DNA methylase, and cloned into a pQE30 vector using dam-/dcm- E. coli as the expression host. The genomic DNA and the plasmid carrying methylase-encoding sequences were extracted and cleaved with restriction enzymes that were sensitive to methylation, to confirm the methylation activity. These methylases protected the restriction enzyme site once IPTG-induced methylases methylated the chromosome and plasmid, harboring the DNA methylase. In this study, cloned exocyclic DNA methylases were investigated for potential use as a novel type of GpC methylase for molecular biology and epigenetics.

• 재활복지
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• v.17 no.4
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• pp.127-149
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• 2013

This study analyzed the hazard of unemployment and the influencing factors on the rate. Data came from the Panel Survey of Employment for the Disabled(PSED), 2010-2012, which is a longitudinal survey for 5,092 disabled people in Korea. For the main purpose of this study, the life-table method was used for describing the patterns of unemployment duration by disabled, and the cox proportional hazard model was used to identify significant factors on the unemployment duration. The results were as follows. First, according to the life table analysis, the unemployment rate to remain until the longest period of unemployment(25month) is 90.5%, and the rate of entry into the labor market was only 9.5%. Overall, the unemployment maintenance rate was high, the unemployment escape rate decreased after 12month. Second, looking at the results from the cox proportional hazards model, the unemployment escape possibility were increased for those who are male, are non-public benefit recipient with disability, have mild disability, and have less discrimination experiences. With these results, disability discrimination act which can reduce the disability discrimination in employment site should be strengthened. Also, the scheme of Nation Basic Protection Program should be modified to attract the employment of recipients with disability. Finally, policy targets having employment escape difficulty, such as women with disability, people with severe disabilities should be departmentalized. And employment service is provided in accord with the individual needs and characteristics.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.05a
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• pp.496-499
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• 2014

Xe Gas is moved to Target from GPM. It is Used to feasible nuclear reaction from proton of 30MeV Cyclotron being investigated by the Xe-124 Gas target System. This system is divided into four parts. Hardware was constructed by solidworks and Helium Supply is to cool the Havor Foil. Water has the job of cooling down the temperature when Xe Gas is being investigated in the target. Temperature and pressure gauges are attached to be checked easily. GPM(Gas Process Manifold) has the part that prepares to transport Xe Gas. There are Storage Vessel that stores Xe Gas, the cold trap that filters humidity and impurity and lastly storage vessel that temporarily stores Xe Gas. HCS(Helium Circulation System) using the Helium to cleaning and cooling. these parts are used to SIEMENS PLC and Pcvue Program. Because It is more comfortable and easy maintenance.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.10a
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• pp.243-246
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• 2016

Data transfer technique is one of the key elements of the IT infrastructure, in accordance with each environment and function, using some kind of protocol. Using the three protocols such as WCDMA, AIS, and TRS at sea IT industry. so It uses three protocols saw has a problem that the operation of each protocol-specific server. so server maintenance costs increase and sea Device Manager has been placed in a situation which operates to develop each of the protocol-specific analysis program. The reason of the protocol is divided into three, marine equipment (lighthouses, buoys, light buoy) manufacturing companies and the date of manufacture, such as, communication environment (distance, communication speed) because such is wrong all, trying to unify this and enormous cost and time is a situation that can not be the exchange occurred when. So in the Ministry of Maritime Affairs and Fisheries, the number of frequency of use is low, communication performance is seeking to refrain from Kure each local organizations and companies the use of low TRS protocol. his paper is intended to conduct research on the basis of the actual data to analyze whether the method is how economical to store a single DB by analyzing other types of protocols. This reduces the shortage of marine IT industry, even a little, shall helps reduce administrative costs.

• The Journal of Korean Society for School & Community Health Education
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• v.20 no.1
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• pp.1-14
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• 2019

Objectives: There are few studies which reflects of each generation's own features, of the New Middle Age and the Elderly's hearing status and their quality of life even though the population of old age has been weighted as baby boom generation's entering into old age. This study is to identify the effects of the new middle age and the elderly's hearing status on their quality of life and to prepare the customized health policies for their health promotion and quality of life improvement as well as the base line data for hearing enhancement programs. Methods: This study was analyzed using the data of the 7th term of the National Health and Nutrition Survey of the Disease Management headquarters. Out of 8,150 adults over the age of 50 who participated in the hearing test, 3,306 were selected and analyzed. Results: At the result of identifying the correlation of hearing status and the quality of life between the New Middle Age and the Elderly, it was confirmed that hearing loss affects low quality of life. The average of quality of life between the New Middle Age and the Elderly was 0.95 and 0.85 each, resulting in higher quality of life in New Middle Age than in the Elderly. The factors affecting the quality of life of the New Middle Age were the level of the education, household income, the type of health insurance, subjective health status. The factors affecting the quality of life of the Elderly were gender, the type of health insurance, subjective health status. Conclusion: Hearing loss of the New Middle Age and the Elderly affects their low quality of life. Therefore the development and the provision of policy program is needed, so as to maintain and manage hearing through age-specific health education. It is expected that the second half of the New Middle Age's life will be much happier, if not taking the treatment-oriented approach of hearing loss only but strengthening the education needed for the maintenance and the management of healthy hearing at their work place, where 50 and 60 generations' workforce has been increased.

• Journal of Ginseng Research
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• v.43 no.2
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• pp.305-311
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• 2019

Background: Gintonin is a ginseng-derived exogenous ligand of the G protein-coupled lysophosphatidic acid (LPA) receptor. We previously reported that gintonin stimulates gliotransmitter release in primary cortical astrocytes. Astrocytes play key roles in the functions of neurovascular systems. Although vascular endothelial growth factor (VEGF) is known to influence the normal growth and maintenance of cranial blood vessels and the nervous system, there is little information about the effect of gintonin on VEGF regulation in primary astrocytes, under normal and hypoxic conditions. Methods: Using primary cortical astrocytes of mice, the effects of gintonin on the release, expression, and distribution of VEGF were examined. We further investigated whether the gintonin-mediated VEGF release protects astrocytes from hypoxia. Results: Gintonin administration stimulated the release and expression of VEGF from astrocytes in a concentration- and time-dependent manner. The gintonin-mediated increase in the release of VEGF was inhibited by the LPA1/3 receptor antagonist, Ki16425; phospholipase C inhibitor, U73122; inositol 1,4,5- triphosphate receptor antagonist, 2-APB; and intracellular $Ca^{2+}$ chelator, BAPTA. Hypoxia further stimulated astrocytic VEGF release. Gintonin treatment stimulated additional VEGF release and restored cell viability that had decreased due to hypoxia, via the VEGF receptor pathway. Altogether, the regulation of VEGF release and expression and astrocytic protection mediated by gintonin under hypoxia are achieved via the LPA receptor-VEGF signaling pathways. Conclusion: The present study shows that the gintonin-mediated regulation of VEGF in cortical astrocytes might be neuroprotective against hypoxic insults and could explain the molecular basis of the beneficial effects of ginseng on the central nervous system.

• Korea Science and Art Forum
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• v.37 no.3
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• pp.135-145
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• 2019

IFC(Industry Foundation Classes) is used for information exchange in BIM-based collaboration process, and it does not secure reliability of information exchange results between fields due to structural limitations. In the end, it is a realistic problem that we have to use specialized BIM software by field, and we can not secure smooth interoperability in the process of information exchange and change. This study was conducted to find ways to secure the interoperability of BIM work between architecture and structure fields by utilizing Open Source provided by software developers to solve these problems. First, through expert survey and in-depth interview, information and improvement factors required in the process of collaboration between architecture and structure were derived. Second, in order to find a solution to the improvement factors, existing studies related to Open-API and domestic and overseas APIs were investigated and analyzed. Third, Ad-On was developed to secure interoperability by using Open API, mainly BIM S/W, which is mainly used in each field. As a result of the study, the possibility of securing interoperability through the management of the design change history between fields was confirmed by using API. It is judged that the application range of API will be expanded to the construction and maintenance field in the future. Therefore, in order to increase the usability for application diffusion, further research on interface improvement through user-centered verification is needed.

• Journal of agricultural medicine and community health
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• v.45 no.4
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• pp.194-207
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• 2020

Purpose: The purpose of this study is to develop and evaluate the curriculum, that was the continuing professional development program, for cultivating physician's competencies in public health. Methods: This study was conducted through in-depth interview and survey in the frame of ADDIE, from 2 May to 30 June in 2019. Participants were 7 physicians by in-depth interview and Respondents were 46 physicians worked in public health by survey. Results: The results were analyzed and described in the frame of ADDIE model. In the stage of Analysis, physician's core competencies in public health was presented to practical education, management of organization and labor force, communication with community, and director' role of public health center in the community as core competencies by in-depth interview. The skill - knowledge-attitude competencies were highest in order in the survey for placing priority (by Borich score) of 29 core competencies. In design stage, the educational objective of curriculum was established, that is to develop the competencies of knowledge, skills, and attitude needed for physician of public health in 21th century. In development and implementation stage, it is important to decide to lecturer, to get in advance educational materials to do the maintenance and management of curriculum. In the stage of evaluation, the educational satisfaction was high on the whole and educational effect was statistically significant. Conclusions: This study was an initial study of Korean doctors, and it aims to pursue competency-based education as a continuing professional development (CPD) beyond continuing medical education (CME) including knowledge, skills, and attitudes.

• Journal of Korea Entertainment Industry Association
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• v.15 no.7
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• pp.145-158
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• 2021

This study is aimed at identifying the important factors required by customers through importance-satisfaction analysis of how they choose skin care shops on the basis of certain factors and attributes at this time when there is an urgent need to improve the level of quality that meets their expectations and demands in combination with the quantitative expansion of skin care shops. To achieve this, a survey was carried out to 217 people over the age of 20 in Gwangju and Jeonnam area with experience in using skin care shop services. The SPSS v. 21.0 program was used to analyze the collected data. The results are summarized as follows. IPA grid analysis of the difference in importance-satisfaction of sub-factors for skin care shop choice showed that the items corresponding to the upper-left side of maintenance and strengthening, the upper-right side of stainability, and the lower-left side of gradual improvement were the 'sanitary control of tools and products', 'basic situation(legal compliance)', and 'workers' sanitary control', 'internal control of skin care shops', 'professionalism and skills of workers'and 'quality of services', respectively. Consequently, the results of this study will be based on the efficient operation of skin care shops, and further more the utilization of them is expected to expand the quantitative and qualitative base of the skin care industry.

• Journal of Digital Convergence
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• v.20 no.5
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• pp.653-659
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• 2022

In this study, the awareness of hazardous chemicals and the need for education of dental hygienists were investigated. The subject of this study was an online survey of 103 dental hygienists working in medical institutions from June to August 2021 and analyzed using the SPSS 21.0 program. As a result, work experience was positively correlated with perception (r=.280, p<0.01) and work (r=.303, p<0.01), and work experience with perception (r=.411, p<0.01).Hazardous chemical information showed a positive correlation with practice (r=.371, p<0.01). As a result of the education requirement survey, only 27.2% had experience in hazardous chemical education, and 96.1% of the awareness of the need for education was highly surveyed. As for the desired method of education, 49.5% of them were online education, and the desired time for education was 1 hour. Therefore, in order to create a safe working environment for dental hygienists and to secure the safety of hazardous chemicals, it was necessary to expand educational opportunities at universities, medical institutions, and maintenance education, and to increase accessibility through online education.