• Natural Product Sciences
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• 제11권2호
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• pp.89-91
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• 2005

Effect of the neolignans, honokiol (1) and magnolol (2), isolated from Magnoliae Cortex on mushroom tyrosinase activity was investigated in vitro using L-tyrosine as a substrate. Honokiol (1) inhibited tyrosinase activity significantly in a concentration-dependent manner, on the other hand, magnolol (2) did not show tyrosinase inhibitory effect. Honokiol exhibited tyrosinase inhibitory effect with $IC_{50}$ value of $67.9\;{\mu}M$, and proved to act as a non-competitive inhibitor by the analysis of Lineweaver-Burk plot.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.260.1-260.1
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• 2003

Magnoliae Flos(‘shin-j’). the flower buds of Magnolia kobus, is acrid to taste with a ‘warm’ property. It is a ‘wind-cold’ discutient and nasal decongestant and is principally used in the treatment of nasal congestion with headache, sinusitis and allergic rhinitis. By screening inhibitory activities on the melanin polymer biosysthesis in B-16 mouse melanoma cell lines, methylene chloride extract of Magnoliae Flos was found to have inhibitory effect on melanin polymer biosynthesis. (omitted)

• Journal of Periodontal and Implant Science
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• 제28권4호
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• pp.545-559
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• 1998

Magnoliae cortex has been used as a drug for treatment of fractures in Chinese medicine and safflower(Carthamus tinctorius $Linn{\acute{e}}$) has been traditionally used for treatment of blood stasis. The purpose of present study was to examine the biologic effects of magnoliae cortex extract and safflower extract mixture(MSM) on human periodontal ligament cells and fetal rat calvarial osteoblasts and on healing of rat calvarial defects. The ethanolic extracts of magnoliae cortex(MCE), safflower seed(SSE), Zea May L(ZML) were prepared as positive control group. MSM mixed to the ratios of 1 : 1, 1 : 2, 1 : 5 and 1 : 10 were used as test group. The effects of each agents on the growth and survival, ALPase activity, cell proliferation and tissue regenerative effect of each extracts was evaluated by histomorphometric measuring of newly formed bone on the 8 mm defect in rat calvaria after oral administration of 2 ratio groups(1 : 5 and 1 : 10) at 3 different doses (0.1, 0.25 and 0.5g/kg per day). MSM stimulated the growth and survival rate of osteoblasts and PDL cells more than any other agents. The growth and survival rate were increased as the proportion of safflower seed extract was increased. MCE, SSE, ZML stimulated the ALPase activity of osteoblast and PDL cell in comparison to the negative control group. But all groups of MSM regardless of ratio of safflower seed extract stimulated the ALPase activity than any other agent. The ALPase activity was also increased as the proportion of safflower seed extract was increased. Although MCE, SSE, ZML stimulated the proliferation of osteoblasts. 1 : 5 and 1 : 10 ratio MSM showed significant increase in stimulation of proliferation of osteoblasts. No agent significantly increased proliferation of PDL cells. Significant new bone formation were seen where 1 : 5 ratio, 0.5g/kg group and 1 : 10 ratio, 0.25, 0.5g/kg groups were used. These results show that magnoliae cortex extract and safflower seed extract mixture can potentially increase bone regeneration ability.

• Journal of Applied Biological Chemistry
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• 제65권1호
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• pp.43-48
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• 2022

많은 약리학적 효과가 입증되고 안전하다고 인정되는 생약제를 포함한 천연물은 오랫동안 사용되어 왔다. 다만 생약제로 사용되는 천연물의 안전성 등 부작용은 명확하게 확인되지 않았다. 이번 연구의 목적은 통증, 비염, 폐렴 치료에 사용되는 신이의 안정성 평가를 확인하기 위하여 SD 랫드 동물모델을 이용하여 4주 반복 용량 결정 독성시험으로 신이의 독성평가를 수행하였다. 수컷과 암컷 SD 랫드에 신이추출물을 28일 동안 1,500, 3,000 mg/kg의 농도로 하루 1회 경구 투여하였다. 신이추출물의 안정성 평가는 일반증상관찰, 체중 및 사료섭취량 측정, 혈구 및 혈액생화학 분석, 부검소견 관찰 및 상대적 장기무게 측정으로 판단하였으며 실험기간동안 신이추출물의 투여로 독성 관련 유의미한 변화는 나타나지 않았다. 따라서 이러한 연구결과는 4주 반복 용량 결정 독성시험에서 신이추출물이 독성 등 부작용은 나타나지 않았으며, 신이추출물의 독성이 나타나지 않는 최대무독성량은 3,000 mg/kg 이상으로 추정되었다.

• 생약학회지
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• 제29권2호
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• pp.136-141
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• 1998

To analyze scientifically the polyprescription principle of Daekumeumja(對金飮子), which has been used for alcoholic damage, and Pyungwesan(平胃散), which has been used for indigestion, the transforming rate of hesperidin of these polyprescriptions to hesperetin was investigated. The transforming rate of the former was higher 3 times than that of the latter. The transforming rate of hesperidin of Aurantii nobilis Pericarpium was inhibited by Magnoliae Cortex, but was activated by Glycyrrhizae Radix. The activity of trypsin was inhibited by Glycyrrhizae Radix and Daekumeuja. However, Aurantii nobilis Pericarpium, Atractylodis Rhizoma, Magnoliae Cortex and Pyungwesan did not inhibited it. When human intestinal microflora were cultured with the media containing Daekumeumja, Pyungwesan and herbal medicines consisting of them, Aurantii nobilis Pericarpium and Magnoliae Cortex inhibited the activity of ${\beta}$-glucosidase. These results suggests that the therapeutic effect of Daekumeumja may be better than that of Pyungwesan on alcoholic damage and the therapeutic effect of Pyungwesan may be better than that of Daekumeumja on indigestion, although these prescriptions are consisted of the same herbal medicines.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.117-122
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• 2014

As a Chinese herbal medicine used in East Asia for thousands years, Cortex Magnoliae Officinalis (CMO) was observed to possess a protective effect against OH-induced DNA damage in the study. To explore the mechanism, the antioxidant effects and chemical contents of five CMO extracts were determined by various methods. On the basis of mechanistic analysis, and correlation analysis between antioxidant effects & chemical contents, it can be concluded that CMO exhibits a protective effect against OH-induced DNA damage, and the effect can be attributed to the existence of phenolic compounds, especially magnolol and honokiol. They exert the protective effect via antioxidant mechanism which may be mediated via hydrogen atom transfer (HAT) and/or sequential electron proton transfer (SEPT). In the process, the phenolic-OH moiety in phenylpropanoids is oxidized to the stable quinine-like form and the stability of quinine-like can be ultimately responsible for the antioxidant.

• 한국약용작물학회지
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• 제13권6호
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• pp.245-249
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• 2005

신이는 전통적으로 비염 등의 치료에 사용되어 왔는데, 신이 에탄올 추출물은 그람 양성균인 Staphylococcus aureus 그람 음성균인 Pseudomonas fluorescens균 및 곰팡이균인 Aspergillus niger에 대하여 모두 항균활성을 보였으며, proteinase로 활성화된 receptor-2에 의한 흰쥐 뒷발바닥 부종모델에서 신이 에탄올 추출물을 경구투여한 경우 typsin이나 $tc-NH_2$로 유발된 발바닥 부종, 혈관투과성, myoloperoxidase 활성도에서 유의성 있는 억제율을 보여 항염증 활성이 뚜렷함을 나타냈다.

• KSBB Journal
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• 제18권4호
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• pp.249-254
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• 2003

Candida magnoliae M26에 의한 erythritol의 산업적 생산을 위한 산업용 질소원의 선정과 이를 이용한 유가식 배양에서의 erythritol 생산을 위한 최적 조건의 결정에 대한 실험을 수행하였다. 실험결과에서 산업용 질소원으로 가격이 가장 저렴하면서 erythritol의 수율과 생산성이 거의 yeast extract와 유사한 light steep water (LSW)를 선정하였다. 즉 선정한 LSW 43.3 mL/L과 glucose 250 g/L을 이용한 회분식 발효에서 52.6％의 수율과 0.52 g/L-hr의 생산성으로 131.6 g/L의 erythritol을 생산하였다. 생산성 향상을 위한 2단계 유가식 배양에서 고농도 균체를 얻기 위한 최적이 C/N ratio는 1.7으로서 glucose 100 g/L와 LSW 500 mL/L를 이용한 회분식 배양에서 71.0 g/L의 세포농도를 얻을 수 있었다. 배지의 초기 pH 조절방법에 대한 연구에서는 28％ 암모니아 용액으로 pH 6.5로 조절할 경우 erythritol의 수율은 감소하였으나 높은 생산성으로 erythritol을 얻을 수 있었다. 따라서 erythritol의 수율 향상을 위한 생산단계에서의 최적 glucose 농도는 450 g/L이 되도록 하는 경우이며, 이때에 37.3％의 수율과 1.66 g/L-hr의 생산성으로 185.5 g/L의 erythritol을 생산할 수 있었다. 따라서 산업용 질소원으로 선정된 LSW를 이용한 2단계 유가식 발효 공정을 통하여 회분식 배양과 비교해 볼 때 수율은 1.4배 정도 감소되었지만 erythritol의 생산성은 3.2배로 향상시킬 수 있었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.351-354
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• 2001

In this experiment, we tested various nitrogen sources and then culture condition was optimized for industrial applications. The batch culture of Candida magnoliae SR101 grown in a defined medium supplemented with light steep water (LSW) as a sole nitrogen source showed a relatively high yield of erythritol production (53%), which was slightly higher than that using yeast extract as a nitrogen source, while the productivity and cell mass were maintained at similar levels. For the optimization of culture condition, the batch culture was performed. When the concentration of LSW was 65 mL/L in the defined medium containing 250 g/L of glucose, the concentration, yield and productivity of erythritol were 110 g/L, 44%, and 0.66 g/L-hr, respectively. The high yield and comparable productivity obtained with a cheap nitrogen source could be expected as a basis for the mass production of erythritol in the industrial scale.

• Natural Product Sciences
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• 제19권2호
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• pp.160-165
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• 2013

The 12 compounds were isolated from MeOH extract of Magnolia biondii and their structures were identified as seven lignans, two phenolics, one coumarin, and two flavonoid compounds, respectively. Among these constituents, tiliroside (3), kaempferol-7-methyl ether (4), 4-hydroxybenzoic acid (5), vanilic acid (6), and scopoletin (9) were isolated from Magnolia biondii for the first time.