• Title/Summary/Keyword: Magnification imaging

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A Phase Unwrapping Algorithm in Three-Wavelength Digital Holographic Microscopy

  • Shin, Sanghoon;Yu, Younghun
    • New Physics: Sae Mulli
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    • v.68 no.12
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    • pp.1378-1383
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    • 2018
  • Multi-wavelength holography has a better axial range than single-wavelength holography, thus allowing unambiguous phase imaging. Noise amplification is the limiting factor in multi-wavelength holography as noise is amplified by a factor equivalent to the magnification of the wavelengths. Here, we propose a new algorithm to remove noise amplification in multi-wavelength holography. The proposed method does not use phase unwrapping and removes $2{\pi}$ ambiguities. Experiments and numerical simulations indicated that the proposed method is fast, has low noise, and is useful for measuring samples with arbitrary step heights.

3D Image Correlator using Computational Integral Imaging Reconstruction Based on Modified Convolution Property of Periodic Functions

  • Jang, Jae-Young;Shin, Donghak;Lee, Byung-Gook;Hong, Suk-Pyo;Kim, Eun-Soo
    • Journal of the Optical Society of Korea
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    • v.18 no.4
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    • pp.388-394
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    • 2014
  • In this paper, we propose a three-dimensional (3D) image correlator by use of computational integral imaging reconstruction based on the modified convolution property of periodic functions (CPPF) for recognition of partially occluded objects. In the proposed correlator, elemental images of the reference and target objects are picked up by a lenslet array, and subsequently are transformed to a sub-image array which contains different perspectives according to the viewing direction. The modified version of the CPPF is applied to the sub-images. This enables us to produce the plane sub-image arrays without the magnification and superimposition processes used in the conventional methods. With the modified CPPF and the sub-image arrays, we reconstruct the reference and target plane sub-image arrays according to the reconstruction plane. 3D object recognition is performed through cross-correlations between the reference and the target plane sub-image arrays. To show the feasibility of the proposed method, some preliminary experiments on the target objects are carried out and the results are presented. Experimental results reveal that the use of plane sub-image arrays enables us to improve the correlation performance, compared to the conventional method using the computational integral imaging reconstruction algorithm.

Reproducibility of panoramic radiography in patients (임상에서 촬영되는 파노라마 방사선사진의 재현성 조사)

  • Nah Kyung-Soo
    • Imaging Science in Dentistry
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    • v.35 no.3
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    • pp.115-119
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    • 2005
  • Purpose : To evaluate the magnification error percentage in repeatedly taken panoramic radiographs of same patient and machine. Materials and Methods : 92 panoramic radiographs from 46 patients were traced and 30 horizontal and vertical measurements were made with digital sliding caliper. The results were compared with paired t-test. Results : There was no statistically significant difference between the two measurements. The overall difference as percentage error was $6.19\pm5.60\%$. The largest error as $14.61\pm12.44\%$ was found at condylar height 1, and smallest as $1.86\pm1.61\%$ at mandibular height. Overall vertical error excluding condylar height 1 was $3.76\pm3.97\%$, and the horizontal error $6.88\pm5.92\%$. Conclusion . Repeatedly taken panoramic radiographs of the same patient and machine was reliable since there was no significant percentage error difference but the percentage error ranged from $1.86\pm1.61\%\;to\;14.61\pm12.44\%$ indicating the error depends on the measuring site.

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Quantitative localization of impacted mesiodens using panoramic and periapical radiographs

  • Choi, Hang-Moon;Han, Jin-Woo;Park, In-Woo;Baik, Jee-Seon;Seo, Hyun-Woo;Lee, Joo-Hyun;Park, Ho-Won
    • Imaging Science in Dentistry
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    • v.41 no.2
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    • pp.63-69
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    • 2011
  • Purpose : The purpose of this study was to evaluate a new technique for localizing impacted mesiodens using its horizontal magnification ratio on panoramic radiographs. Materials and Methods : Location-magnification equation of a panoramic equipment was obtained from horizontal magnification ratio of a metal ball which was located variable positions from the center of image layer at interval of 2 mm. Panoramic radiographs were obtained from a skull phantom with a metal ball which was a substitute for impacted mesiodens and was embedded 10mm(Group 1), 15mm(Group 2), and 20mm(Group 3) posterior to the central incisor. Each group obtained 7 panoramic radiographs at variable positions and one periapical radiograph. Three methods were used to estimate the actual width of the incisors and the balls which were used to calculate the magnification ratio. The methods included using the actual incisor width and the calculated ball width (Method 1), using the actual incisor width and the ball widths measured on periapical radiograph (Method 2), and using the incisor and the ball widths measured on periapical radiograph (Method 3). The location of the metal ball was calculated by using the location-magnification equation. Results : The smallest difference between the calculated and the actual distance was $0.1{\pm}0.7 \;mm$ in Group 1/ Method 3. The largest difference was $-4.2{\pm}1.6 \;mm$ in Group 3/Method 2. In all groups, method 3 was the most accurate. Conclusion : Quantitative localization of impacted mesiodens is possible by using panoramic radiograph.

Enlargement of Field-of-View (FOV) of the CCD Camera by the Current Adjustment of the Projection Lens System in the KBSI-HVEM (KBSI-HVEM 투사렌즈 전류제어에 의한 CCD Camera의 Field of View(FOV) 확장)

  • Kim, Young-Min;Shim, Hyo-Sik;Kim, Youn-Joong
    • Applied Microscopy
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    • v.35 no.4
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    • pp.98-104
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    • 2005
  • A FOV (field-of-view) of the HV-MSC (high voltage multi-scan CCD, $1024{\times}1024$ pixels) camera mounted in the post-column HV-GIF (high voltage gatan image filter) has been drastically enlarged by the projection lens current adjustment. An imaging area of the HV-MSC camera obtained at the lowest magnification (2,000x) is $112{\mu}m^2$ which corresponds to the recording area of the film at the magnification of 8,800x, while the achievable recording area is only $0.43{\mu}m^2$ at the same magnification without this technique. Ignoring the image distortion of less than 5%, we have designed an on-site reference graph to estimate projection lens currents for microscope magnifications above 8,800x, where the recording area on the HVMSC is same as that on the film.

Lymphovenous anastomoses with three-dimensional digital hybrid visualization: improving ergonomics for supermicrosurgery in lymphedema

  • Will, Patrick A.;Hirche, Christoph;Berner, Juan Enrique;Kneser, Ulrich;Gazyakan, Emre
    • Archives of Plastic Surgery
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    • v.48 no.4
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    • pp.427-432
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    • 2021
  • The conventional approach of looking down a microscope to perform microsurgical procedures is associated with occupational injuries, anti-ergonomic postures, and increased tremor and fatigue, all of which predispose microsurgeons to early retirement. Recently, three-dimensional (3D) visualization of real-time microscope magnification has been developed as an alternative. Despite its commercial availability, no supermicrosurgical procedures have been reported using this technology to date. Lymphovenous anastomoses (LVAs) often require suturing vessels with diameters of 0.2-0.8 mm, thus representing the ultimate microsurgical challenge. After performing the first documented LVA procedure using 3D-augmented visualization in our unit and gaining experience with this technique, we conducted an anonymized in-house survey among microsurgeons who had used this approach. The participants considered that 3D visualization for supermicrosurgery was equivalent in terms of handling, optical detail, depth resolution, and safety to conventional binocular magnification. This survey revealed that team communication, resident education, and ergonomics were superior using 3D digital hybrid visualization. Postoperative muscle fatigue, tremor, and pain were also reduced. The major drawbacks of the 3D visualization microscopic systems are the associated costs, required space, and difficulty of visualizing the lymphatic contrast used.

Synchrotron Radiation Imaging of Tissues Using Phase Contrast Technique (방사광 위상차 현미경을 이용한 생체조직의 미세구조 영상)

  • Kang, Bo-Sun;Lee, Dong-Yeol;Kim, Ki-Hong
    • Journal of the Korean Society of Radiology
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    • v.2 no.2
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    • pp.23-30
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    • 2008
  • X-ray microscopy with synchrotron radiation(SR) might be a useful tool for novel x-ray imaging in the clinical and laboratory settings. Microscopically, it enables us to observe detailed structure of animal organs samples with a great magnification power and an excellent resolution. The phase contrast mechanisms in image by X-ray are described. The phase-contrast X-ray imaging with SR from in-vivo and in-vitro mouse tail, rat nerve and rat lung were obtained with an 8 KeV monochromatic beam. The visual image was magnified using 10x microscope objective lens and captured using an digital CCD camera. The results showed more structural details and high resolution images with SR imaging system than conventional X-ray radiography system. The SR imaging system may have a potential for imaging in biological researches, material applications and clinical radiography.

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List-event Data Resampling for Quantitative Improvement of PET Image (PET 영상의 정량적 개선을 위한 리스트-이벤트 데이터 재추출)

  • Woo, Sang-Keun;Ju, Jung Woo;Kim, Ji Min;Kang, Joo Hyun;Lim, Sang Moo;Kim, Kyeong Min
    • Progress in Medical Physics
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    • v.23 no.4
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    • pp.309-316
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    • 2012
  • Multimodal-imaging technique has been rapidly developed for improvement of diagnosis and evaluation of therapeutic effects. In despite of integrated hardware, registration accuracy was decreased due to a discrepancy between multimodal image and insufficiency of count in accordance with different acquisition method of each modality. The purpose of this study was to improve the PET image by event data resampling through analysis of data format, noise and statistical properties of small animal PET list data. Inveon PET listmode data was acquired as static data for 10 min after 60 min of 37 MBq/0.1 ml $^{18}F$-FDG injection via tail vein. Listmode data format was consist of packet containing 48 bit in which divided 8 bit header and 40 bit payload space. Realigned sinogram was generated from resampled event data of original listmode by using adjustment of LOR location, simple event magnification and nonparametric bootstrap. Sinogram was reconstructed for imaging using OSEM 2D algorithm with 16 subset and 4 iterations. Prompt coincidence was 13,940,707 count measured from PET data header and 13,936,687 count measured from analysis of list-event data. In simple event magnification of PET data, maximum was improved from 1.336 to 1.743, but noise was also increased. Resampling efficiency of PET data was assessed from de-noised and improved image by shift operation of payload value of sequential packet. Bootstrap resampling technique provides the PET image which noise and statistical properties was improved. List-event data resampling method would be aid to improve registration accuracy and early diagnosis efficiency.

Utility of narrow-band imaging with or without dual focus magnification in neoplastic prediction of small colorectal polyps: a Vietnamese experience

  • Tien Manh Huynh;Quang Dinh Le;Nhan Quang Le;Huy Minh Le;Duc Trong Quach
    • Clinical Endoscopy
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    • v.56 no.4
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    • pp.479-489
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    • 2023
  • Background/Aims: Accurate neoplastic prediction can significantly decrease costs associated with pathology and unnecessary colorectal polypectomies. Narrow-band imaging (NBI) and dual-focus (DF) mode are promising emerging optical technologies for recognizing neoplastic features of colorectal polyps digitally. This study aimed to clarify the clinical usefulness of NBI with and without DF assistance in the neoplastic prediction of small colorectal polyps (<10 mm). Methods: This cross-sectional study included 530 small colorectal polyps from 343 consecutive patients who underwent colonoscopy at the University Medical Center from September 2020 to May 2021. Each polyp was endoscopically diagnosed in three successive steps using white-light endoscopy (WLE), NBI, and NBI-DF and retrieved for histopathological assessment. The diagnostic accuracy of each modality was evaluated with reference to histopathology. Results: There were 295 neoplastic polyps and 235 non-neoplastic polyps. The overall accuracies of WLE, WLE+NBI, and WLE+NBI+NBI-DF in the neoplastic prediction of colorectal polyps were 70.8%, 87.4%, and 90.8%, respectively (p<0.001). The accuracy of WLE+NBI+NBI-DF was significantly higher than that of WLE+NBI in the polyp size ≤5 mm subgroup (87.3% vs. 90.1%, p<0.001). Conclusions: NBI improved the real-time neoplastic prediction of small colorectal polyps. The DF mode was especially useful in polyps ≤5 mm in size.