• Title/Summary/Keyword: Magnesium ($Mg^{++}$)

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Changes in hematological parameters and plasma components of olive flounder, Paralichthys olivaceus exposed to acute microplastics (넙치, Paralichthys olivaceus의 미세플라스틱 급성 노출에 따른 혈액성상 및 혈장성분의 변화)

  • Kim, So-Hee;Kim, Ga-Hyun;Kim, Ji-Su;Kim, Jun-Hwan;Jeon, Yu-Hyeon;Cho, Jae-Hwang;Kim, Seok-Ryel;Kim, Dae-Hee
    • Korean Journal of Environmental Biology
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    • v.39 no.3
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    • pp.344-353
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    • 2021
  • Juvenile olive flounder, Paralichthys olivaceus (mean weight 66.7±7.1 g; mean length 19.2±0.9 cm) in a bio-floc environment were exposed to microplastic (PE: polyethylene, size 40-48 ㎛) at 0, 4, 20, 100, 500 and 2,500 mg L-1 for 96 hours. No P. olivaceus deaths were observed following microplastic exposure. In the plasma components, calcium was significantly decreased whereas there was no significant change with magnesium following microplastic exposure. Glucose was significantly decreased with over 100mg L-1 at 48 hours and 20mg L-1 at 96 hours. Cholesterol was significantly decreased with over 20mg L-1 after 48 hours, whereas there was no significant change in the total protein content. In enzymatic plasma components, the AST(Aspartate aminotransferase) was significant decreased by microplastic exposure. The results of this study indicate that acute exposure to microplastic induces blood physiological changes in P. olivaceus.

Development of Analytical methods for Chinomethionat in Livestock Products (축산물 중 살균제 Chinomethionat의 개별 잔류분석법 확립)

  • Yang, Seung-Hyun;Kim, Jeong-Han;Choi, Hoon
    • Korean Journal of Environmental Agriculture
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    • v.40 no.2
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    • pp.134-141
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    • 2021
  • BACKGROUND: The analytical method was established for determination of fungicide chinomethionat in several animal commodities using gas chromatography (GC) coupled with electron capture detector (ECD). METHODS AND RESULTS: In order to verify the applicability, the method was optimized for determining chinomethonat in various livestock products including beef, pork, chicken, milk and egg. Chinomethionat residual was extracted using acetone/dichloromethane(9/1, v/v) with magnesium sulfate and sodium chloride (salting outassociated liquid-liquid extraction). The extract was diluted by direct partitioning into dichloromethane to remove polar co-extractives in the aqueous phase. The extract was finally purified with optimized silica gel 10 g. CONCLUSION: The method limit of quantitation (MLOQ) was 0.02 mg/kg, which was in accordance with the maximum residue level (MRL) of chinomathionate as 0.05 mg/kg in livestock product. Recovery tests were carried out at two levels of concentration (MLOQ, 10 MLOQ) and resulted in good recoveries (84.8~103.0%). Reproducibilities were obtained (Coefficient of variation <5.2%), and the linearity of calibration curves were reasonable (r2>0.995) in the range of 0.01-0.2 ㎍/mL. This established analytical method was fully validated and could be useful for quantification of chinomathionat in animal commodities as official analytical method.

Synthesis of akermanite bioceramics by solid-state reaction and evaluation of its bioactivity (고상반응법에 의한 아커마나이트 분말의 합성 및 생체활성도 평가)

  • Go, Jaeeun;Lee, Jong Kook
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.32 no.5
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    • pp.191-198
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    • 2022
  • Zirconia and titanium alloys, which are mainly used for dental implant materials, have poor osseointegration and osteogenesis abilities due to their bioinertness with low bioactivity on surface. In order to improve their surface bioinertness, surface modification with a bioactive material is an easy and simple method. In this study, akermanite (Ca2MgSi2O7), a silicate-based bioceramic material with excellent bone bonding ability, was synthesized by a solid-state reaction and investigated its bioactivity from the analysis of surface dissolution and precipitation of hydroxyapatite particles in SBF solution. Calcium carbonate (CaCO3), magnesium carbonate (MgCO3), and silicon dioxide (SiO2) were used as starting materials. After homogeneous mixing of starting materials by ball milling and the drying of at oven, uniaxial pressing was performed to form a compacted disk, and then heat-treated at high temperature to induce the solid-state reaction to akermanite. Bioactivity of synthesized akermanite disk was evaluated with the reaction temperature from the immersion test in SBF solution. The higher the reaction temperature, the more pronounced the akermanite phase and the less the surface dissolution at particle surface. It resulted that synthesized akermanite particles had high bioactivity on particle surface, but it depended on reacted temperature and phase composition. Moderate dissolution occurred at particle surfaces and observed the new precipitated hydroxyapatite particles in synthetic akermanite with solid-state reaction at 1100℃.

Growth Environment and Vegetation Structure of Habitats of Acer tegmentosum Maxim. (산겨릅나무(Acer tegmentosum Maxim.) 자생지의 서식환경 특성 및 식생구조)

  • Son, Ho-Jun;Kim, Se-Chang;Lee, Da-Hyun;Kwon, Soon-Jae;Park, Wan-Geun;Kim, Young-Seol
    • Journal of agriculture & life science
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    • v.50 no.3
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    • pp.69-80
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    • 2016
  • The present study was to survey the site environment, vegetation structure and soil characteristics in the wild habitats of Acer tegmentosum Maxim. and offers basic information for habitats conservation and restoration. Most of the wild habitats were located at altitudes between 605~1,413m with inclinations ranged as 8~30°. The bare rock rate were 8~50%. The vegetation structure by the PC-ORD based on the Two Way Cluster Analysis were divided into three groups Community I(Acer tegmentosum - Quercus mongolica), Community II(Acer tegmentosum - Carpinus cordata), Community III(Quercus mongolica - Tilia amurensis). The species diversity(H') was highest in Community II as 1.474, Community I was 1.471, Community III was 1.219. The soil textures were Clay loam, The average soil pH was 4.8, Soil organic matter was 15.15% and available phosphorus was 2.33ppm. Ordination analysis result by soil characteristics, community, characteristic species showed that the greatest effect factors were slope, altitude, tree and shrub's cover rate, organic matter, total-nitrogen, calcium, magnesium. Correlation analysis between environment factor result showed that O.M. - (T.N., K+, Mg2+, CEC, EC), T.N. - (K+, Mg2+, CEC, EC) were positive correlations.

Nutritional Properties of Cookies Made with Israeli Carp Cyprinus carpio (향어(Cyprinus carpio) 함유 쿠키의 영양특성)

  • Ye Youl Kim;Mi-Soon Jang;Jae-Young Oh;Sang In Kang;Sun Young Park;Yu Ri Choe;Ji Hoon Park;Si Hyeong Park;Jin-Soo Kim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.6
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    • pp.766-772
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    • 2023
  • This study was conducted to compare the nutritional properties of cookies made with Israeli carp Cyprinus carpio (C-IC) to those made without Israeli carp (control). The proximate composition of C-IC per 100 g was 4.1 g moisture, 9.7 g protein, 29.2 g lipid, 1.4 g ash, and 55.6 g carbohydrates. Moisture, protein and ash contents were significantly higher and the carbohydrate content was significantly lower (P<0.05) in C-IC than control, but there was no difference in lipid content (P>0.05). The total amino acid content of C-IC per 100 g was 9.46 g and the major amino acid was glutamic acid (2.49 g). The mineral contents of C-IC per 100 g were 216.6 mg calcium, 193.2 mg phosphorus, 170.9 mg potassium, and 18.2 mg magnesium, which were all significantly higher than the contents of the control (P<0.05). The major fatty acids of C-IC were 16:0, 18:1n-9, and 18:2n-6. The digestibility of C-IC in the small intestine was 51.3%, which was higher than the digestibility of the control. These results suggest that C-IC have better nutritional properties than the control.

Nutrient Absorption Pattern by Analysis of Drainage through Growth Stages in Cucumber Coir Bag Culture (오이 코이어 자루재배시 배액분석을 통한 생육단계별 적정 양분흡수패턴 구명)

  • Kim, Sung Eun;Lee, Jae Eun;Sim, Sang Youn;Kim, Young Shik
    • Journal of Bio-Environment Control
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    • v.23 no.3
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    • pp.229-234
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    • 2014
  • We analyzed drainage water from coir substrate in which cucumber plants were grown in winter and elucidated changes in pH, EC, and major nutrients according to the growth stages to recommend nutrient solution management appropriate to each growth stage. From the analysis of drainage solution the growth stages of cucumber were desirable to be divided into two, planting to fruit setting and fruit setting to harvest in case of nutrient solution management. The time required was about 3 weeks from planting to the first fruit setting and thereafter 7~10 days more until the first harvest. Approximately every 3~4 days were needed until the upper flowers bloomed. The time required from fruit setting to harvest was not different much among flowers as cucumber plants grew. From the experimental results, EC of supplied solution was recommended to maintain a little high to $3.0dS{\cdot}m^{-1}$ until before fruit setting and lower a little to $2.0{\sim}2.3dS{\cdot}m^{-1}$ after that. Of course, the amount of solution supply should be increased as plants grew. In case of each nutrients, the recommendation of concentrations of nitrogen, phosphorus and calcium were 700, 60, and $110mg{\cdot}L^{-1}$ each until before fruit setting, and then 660, 50, and $100mg{\cdot}L^{-1}$ each after fruit setting. The concentrations of potassium and magnesium are recommended to start from 400 and $80mg{\cdot}L^{-1}$ until fruit setting and lower a little after that.

Analysis of Nutritional Components and Antioxidant Activity of Roasting Wooung (Burdock, Arctium lappa L.) and Jerusalem Artichoke (Helianthus tuberosus L.) (볶음 처리한 우엉과 돼지감자의 영양성분 및 항산화 활성)

  • Lee, Youn Ri
    • The Korean Journal of Food And Nutrition
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    • v.29 no.6
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    • pp.870-877
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    • 2016
  • In this study, we evaluated the nutritional components and functional activities of Wooung (burdock, Arctium lappa L.) and Jerusalem artichoke (Helianthus tuberosus L.) tea. Roasting burdock' contained 75.87% carbohydrates; in addition, the moisture content, crude fat, crude protein, and crude fiber were 10.43%, 1.77%, 8.50%, and 3.43%, respectively. Roasting Jerusalem artichoke showed 77.477% carbohydrate content, with moisture content, crude fat, crude protein, and crude fiber of 10.67%, 1.23%, 7.83%, and 2.80%, respectively. Roasting burdock's water-soluble dietary fiber content was 4.8 g/100 g and insoluble dietary fiber content was 1.5 g/100 g; whereas, roasting Jerusalem artichoke' water soluble dietary fiber content was 2.4 g/100 g and insoluble dietary fiber content was 1.6 g/100 g. The highest mineral contents in roasting burdock and Jerusalem artichoke were potassium and magnesium, in order. The results of amino acid analyses s indicated a total of 25 types in roasting burdock, with total amino acid content of 1,382.112 mg/100 g, and essential amino acid content of 766.031 mg/100 g. In total, 24 types of amino acids were separated and identified in roasting Jerusalem artichoke, with total amino acid content of 2,678.018 mg/100 g, and total essential amino acid content of 157.294 mg/100 g. Roasting burdock and Jerusalem artichoke' polyphenol contents were 32.56 and 29.56 mg GAE/g each, and their flavonoid contents were 16.54 and 16.71 CE/g each. $IC_{50}$ values of DPPH radical-scavenging activity of roasting burdock and Jerusalem artichoke were 12.99 and 19.74, respectively; and $IC_{50}$ values of hydroxyl radical-scavenging activity were 25.96 and 22.93, respectively.

A Study on the Adsorption and Desorption Characteristics of Metal-Impregnated Activated Carbons with Metal Precursors for the Regeneration and Concentration of Ammonia (암모니아의 재생 및 농축을 위한 금속 전구체에 따른 금속 첨착 활성탄의 흡착 및 탈착 특성에 관한 연구)

  • Cho, Gwang Hee;Park, Ji Hye;Rasheed, Haroon Ur;Yoon, Hyung Chul;Yi, Kwang Bok
    • Clean Technology
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    • v.26 no.2
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    • pp.137-144
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    • 2020
  • Metal-impregnated activated carbons were prepared via ultrasonic-assisted impregnation method for regeneration and low ammonia concentration. Magnesium and copper were selected as metals, while chloride (Cl-) and nitrate (NO3-) precursors were used to impregnate the surface of activated carbon. The physical and chemical properties of the prepared adsorbents were characterized by TGA, BET, and NH3-TPD. The ammonia breakthrough test was carried out using a fixed bed and flowing ammonia gas (1000 mg L-1 NH3, balanced N2) at 100 mL min-1, under conditions of temperature swing adsorption (TSA) and pressure swing adsorption (PSA, 0.3, 0.5, 0.7, 0.9 Mpa). The adsorption and desorption performance of ammonia were in the order of AC-Mg(Cl) > AC-Cu(Cl) > AC-Mg(N) > AC-Cu(N) > AC through NH3-TPD and TSA and PSA processes. AC-Mg(Cl) using MgCl2 showed the average adsorption amount of 2.138 mmol/g at TSA process. Also, AC-Mg(Cl) showed the highest initial adsorption amount of 3.848 mmol/g at PSA 0.9 Mpa. When metal impregnated the surface of the activated carbon, it was confirmed that not only physical adsorption, but also chemical adsorption increased, making enhancement in adsorption and desorption performances possible. Also, the prepared adsorbents showed stable adsorption and desorption performances despite repeated processes, confirming their applicability in the TSA and PSA processes.

Changes in Antioxidant Contents and Activities of Adzuki Beans according to Germination Time (발아시간에 따른 팥의 항산화성분 및 항산화활성의 변화)

  • Woo, Koan Sik;Song, Seuk Bo;Ko, Jee Yeon;Lee, Jae Saeng;Jung, Tae Wook;Jeong, Heon Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.5
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    • pp.687-694
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    • 2015
  • The purpose of this study was to evaluate the yield, antioxidant content, and antioxidant activity of adzuki beans according to germination time. Cultivated varieties were Vigna angularis var. Nipponensis cv. Chungju-pat (CJP), and Yeonduchae (YDC), and Vigna radiata (L.) R. Wilczek cv. Dahyeon (DH). The moisture, crude protein, calcium, and magnesium contents of YDC significantly changed with increasing germination time, whereas potassium, natrium, and aluminium contents did not significantly change. Sprout yield, total polyphenol, flavonoid, and tannin contents of ethanolic extracts from adzuki and mung beans significantly increased with increasing germination time. Total polyphenol contents of ungerminated CJP, YDC, and DH were 1.96, 2.68, and 2.02 mg/g, and those of CJP and YDC germinated for 144 h were 3.33 and 3.47 mg/g, respectively. Total flavonoid content of adzuki beans substantially decreased with increasing germination time. Total tannin content substantially increased with increasing germination time, and YDC showed higher contents (0.85 mg/g) sample germinated for 120 h. DPPH radical scavenging activities of CJP and YDC substantially decreased with increasing germination time, whereas that of DH increased. ABTS radical scavenging activities of ungerminated CJP, YDC, and DH were 4.57, 6.51, and 2.82 mg/g, respectively, and increased after germination for 72~120 h.

Component Analysis and Antioxidant Effects of Youngia sonchifola Max. (고들빼기의 성분분석과 항산화효과)

  • Kim, Mee-Jeong;Park, Hee-Suk;Lee, Chang-Il;Kim, Sung-Hwan;Kim, Pil-Nyeon;Huh, Wan;Lee, Do-Yeong;Son, Jin-Chang
    • Journal of Food Hygiene and Safety
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    • v.25 no.4
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    • pp.354-359
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    • 2010
  • In the present study, we investigated the chemical composition, antioxidant activities and nitrite scavenging ability in leaf and root of Youngia sonchifolia Max. The leaf powder contained 4.3% of water, 53.9% of crude carbohydrate, 21.6% of crude protein, 3.5% of crude fat and 16.7% of crude ash. The root powder contained 4.8% of water, 65.9% of crude carbohydrate, 17.4% of crude protein, 3.2% of crude fat and 8.7% of crude ash. The major mineral elements both in leaf and root powder were potassium, calcium, and magnesium. Contents of unsaturated fatty acids were higher than those of saturated fatty acids both in leaf and root powder. Total polyphenol and flavonoid contents of methanol extract in leaf were 3,922.4 mg/100 g and 1,903.2 mg/100 g respectively. In comparison, total polyphenol and flavonoid contents of methanol extract in root powder were 1,898.4 mg/100 g and 359.8 mg/100 g. The antioxidative activities of several solvents extract of leaf and root powder were investigated by measuring electron-donating ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Of the each extracts, ethyl acetate extract of leaf and root powder showed relatively higher antioxidant activity; 94.3% in the leaf powder and 92.9% in the root powder. Nitrite scavenging ability was also highest in the ethyl acetate extract of leaf (45.4%) and root powder (28.8%). These results suggest that ethyl acetate extract of Youngia sonchifolia Max. can be used as a functional materials.