• 제목/요약/키워드: MYO7A

검색결과 114건 처리시간 0.024초

Prediction of Etch Profile Uniformity Using Wavelet and Neural Network

  • Park, Won-Sun;Lim, Myo-Taeg;Kim, Byungwhan
    • International Journal of Control, Automation, and Systems
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    • 제2권2호
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    • pp.256-262
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    • 2004
  • Conventionally, profile non-uniformity has been characterized by relying on approximated profile with angle or anisotropy. In this study, a new non-uniformity model for etch profile is presented by applying a discrete wavelet to the image obtained from a scanning electron microscopy (SEM). Prediction models for wavelet-transformed data are then constructed using a back-propagation neural network. The proposed method was applied to the data collected from the etching of tungsten material. Additionally, 7 experiments were conducted to obtain test data. Model performance was evaluated in terms of the average prediction accuracy (APA) and the best prediction accuracy (BPA). To take into account randomness in initial weights, two hundred models were generated for a given set of training factors. Behaviors of the APA and BPA were investigated as a function of training factors, including training tolerance, hidden neuron, initial weight distribution, and two slopes for bipolar sig-moid and linear function. For all variations in training factors, the APA was not consistent with the BPA. The prediction accuracy was optimized using three approaches, the best model based approach, the average model based approach and the combined model based approach. Despite the largest APA of the first approach, its BPA was smallest compared to the other two approaches.

Association of selected gene polymorphisms with thermotolerance traits in cattle - A review

  • Hariyono, Dwi Nur Happy;Prihandini, Peni Wahyu
    • Animal Bioscience
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    • 제35권11호
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    • pp.1635-1648
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    • 2022
  • Thermal stress due to extreme changes in the thermal environment is a critical issue in cattle production. Many previous findings have shown a decrease in feed intake, milk yield, growth rate, and reproductive efficiency of cattle when subjected to thermal stress. Therefore, selecting thermo-tolerant animals is the primary goal of the efficiency of breeding programs to reduce those adverse impacts. The recent advances in molecular genetics have provided significant breeding advantages that allow the identification of molecular markers in both beef and dairy cattle breeding, including marker-assisted selection (MAS) as a tool in selecting superior thermo-tolerant animals. Single-nucleotide polymorphisms (SNPs), which can be detected by DNA sequencing, are desirable DNA markers for MAS due to their abundance in the genome's coding and non-coding regions. Many SNPs in some genes (e.g., HSP70, HSP90, HSF1, EIF2AK4, HSBP1, HSPB8, HSPB7, MYO1A, and ATP1A1) in various breeds of cattle have been analyzed to play key roles in many cellular activities during thermal stress and protecting cells against stress, making them potential candidate genes for molecular markers of thermotolerance. This review highlights the associations of SNPs within these genes with thermotolerance traits (e.g., blood biochemistry and physiological responses) and suggests their potential use as MAS in thermotolerant cattle breeding.

Streptomyces coelicolor의 3-Phytase 상동성 유전자 ID1103135의 기능분석 (Functional Analysis of Gene ID1103135 Encoding a 3-Phytase Precursor Homologue of Streptomyces coelicolor)

  • 김미순;강대경;이홍섭;연승우;김태영;홍순광
    • 미생물학회지
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    • 제40권2호
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    • pp.81-86
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    • 2004
  • Streptomyces coelicolor의 전 유전체 청보를 분석한 결과(7), 유전자 ID1103135가 코드 하는 open reading frame SCO7697이 phytase[myo-inositol hexakisphosphate phosphohydrolase상동성 (3-6,8,23)]에 유의하게 유사한 것으로 판단되었다. S. coelicolor A3(2)M의 염색체 DNA를 주형으로 PCR 방법으로 SCO7697 전체를 포함하는 DNA 단편을 클로닝하였다. 두 가지의 서로 다른 길이를 갖는 클로닝 된 ID1103135 DNA 단편을 E. coli 발현용 벡터pET728a(+)에 삽입하여,두 종의 재조합 벡터 pET28-SP와 pET28-LP를 얻었다. pET28-SP 와 pET28-LP를 각각 E. coli BL2l에 도입하여, IPTG로 발현 유도된 단백질을 SDS-polyacrylamide 전기영동으로 확인한 결과, 발현은 성공적으로 이루어 졌으나 대부분불용체를 형성하고 분자량은 예상보다 약간 큰 것으로 나타났다. 불용체 형성은 단백질의 불활성화를 수반 함으로서, 배양 온도를 $37^{\circ}C$에서 $30^{\circ}C$로 변화시켜 배양하는 방법으로 발현된 단백질을 가용화 시켰다. 발현된 단백질을 추출하여 조추출물 또는 정제한 상태로 phytase활성을 측청하였으나 효소활성은 관찰할 수 없었다. 대장균 시스템에서의 발현이 효소 활성의 소실을 초래했을 가능성이 있으므로, ID1103135 유전자를 자신의 promoter를 함유하도록 PCR 클로닝하여, E. coli - Streptomyces의 shuttle vector인 pWHM3에 삽입하고, 이를 방선균 호스트인 S. lividans에 도입하였다. 형질 전환체의 세포조추출액 및 세포배양액의 phytase 활성을 측청하였으나, 역시 활성을 확인할수 없었다. 이와 같은 결과는 SCO7697이 아주 높은 확률(E value; $6e^{-89}$)로 phytase일 것으로 annotation 되었으나, 실제는 이와는 다른 기능을 함유하고 있음을 시사하고 있다.

한국에 거주하는 북한 이탈 주민의 명절음식에 대한 인식도 조사 (Awareness Survey on Korean Traditional Festival Food of North Korean Defectors Living in South Korea)

  • 최미경;김묘정;강명화
    • 동아시아식생활학회지
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    • 제25권4호
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    • pp.565-573
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    • 2015
  • The study interviewed 102 North Korean defectors residing in South Korea after completing the Hanawon program through face-to-face interviews. The most celebrated holiday in South Korea was found to be Seollal at 65.7%, followed by Chuseok at 23.2%, Christmas at 7.8%, and Hansik at 1%. With respect to the question of wheather or not North Korea creates a more festive mood compared to South Korea, 33.3% of respondents answered that they felt similar in both countries 2.6% said "yes", they were in a more festive mood in North Korea, whereas 21.6% said "no", and 18.6% said they felt "very different". The most representative traditional food was ranked in the order of rice dishes with kimchi (19.6%), rice cake (11.8%) and boiled rice (8.9%) on National Liberation Day of Korea; rice dishes (17.6%), rice cake (7.8%) and boiled rice (4.9%) on North Korea Constitution Day; and rice cake (57.8%), noodles (9.8%), dumplings (9.8%) and boiled rice (8.8%) on Lunar New Year's Day. In regard to positive recognition about festival foods, "festival food of South Korea has diverse recipes" showed the highest positively in South Korea. The respondents positively recognized that festival foods of North Korea are not sweet, have a unique taste are traditional and have a table setting.

폐경 중년여성의 건강상태 및 삶의 질의 변화 양상: 최근 10년 국민건강영양조사 제4기(2007~2009)와 제7기(2016~2018) 자료 이용 (Changes in Health Status and Quality of Life in Middle-aged Women in Menopause: Using Data from the 4th (2007~2009) and 7th (2016~2018) Korea National Health and Nutrition Examination Survey over the Past 10 Years)

  • 김묘경;오두남
    • 근관절건강학회지
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    • 제30권2호
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    • pp.83-92
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    • 2023
  • Purpose: This study aims to compare and analyze the changes in the physical and mental health status of middle-aged women before and after menopause and their quality of life over the past 10 years. Methods: A descriptive study was conducted by using secondary data from the 4th and 7th Korea National Health and Nutrition Examination Survey (KNHANES) involving middle-aged women in menopause (N=8,363). Data were analyzed using the complex sample x2 test. Results: Compared to 10 years ago, notable changes were observed in general characteristics. These included increase in age, education level, economic activity, and urban residency. In addition, there was an increase in alcohol consumption, a decrease in smoking, and a decline in regular exercise. The study also observed changes in obesity rates and an increase in dyslipidemia and stress levels, along with positive changes across all aspects of quality of life. Conclusion: The results indicate temporal changes in general characteristics, major physical and mental health factors, and the quality of life of middle-aged women in menopause. Thus, it is essential to consider these changes when designing health interventions such as health promotions and education initiatives for middle-aged women experiencing menopause. Further research is necessary for identifying factors influencing the quality of life of middle-aged women in menopause.

휴대폰 동영상 촬영을 활용한 교육이 간호학생의 핵심기본간호술 수행 자신감, 성취도, 실습 만족도에 미치는 효과 (Effects of Core Basic Nursing Education using Cellular Phone Video Recordings on Self-confidence in Performance, Achievement, and Practice Satisfaction)

  • 김묘경;이형숙
    • 기본간호학회지
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    • 제24권2호
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    • pp.128-137
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    • 2017
  • Purpose: This study was conducted to investigate the effects of core basic nursing education using cellular phone video recordings on self-confidence in performance, achievement, and practice satisfaction. Methods: The research design was a nonequivalent control group modified and non-synchronized design. The participants were assigned to either the experimental (n=60) or control group (n=67). During one semester, the experimental group received training for 10 selected core basic nursing skills using cellular phone video recording. The control group, also for one semester, received training with traditional practices. Self-confidence in performance and practice satisfaction were measured using a questionnaire, and achievement was evaluated by professors at the conclusion of the semester. Collected data were analyzed using t-test and Pearson correlation coefficients. Results: Self-confidence in performance increased significantly after training in the experimental group (t=7.94, p<.001). The experimental group showed significantly higher self-confidence in performance (t=2.39, p=.018) and achievement (t=2.40, p=.018) compared to the control group. However, the difference in practice satisfaction was not statistically significant. Conclusion: The results indicate that cellular phone video recording is effective in improving self-confidence in performance and achievement of core basic nursing skills.

다수준 분석을 이용한 청소년의 학교 내 손상 관련 요인 (Factors associated with Unintentional Injuries to Korean Adolescents at School: A Multilevel Study)

  • 유정옥;김묘성
    • 지역사회간호학회지
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    • 제27권4호
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    • pp.337-345
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    • 2016
  • Purpose: This study aims at identifying individual- and school-level factors associated with unintentional injuries to Korean adolescents at school by applying multilevel modeling. Methods: From the database of the eleventh Korea Youth Risk Behavior Web-based Survey (KYRBS), the researchers selected 68,043 adolescents from the $7^{th}$ to the $12^{th}$ grades. Data were analyzed using ${\chi}^2$ test for prevalence and multilevel modeling for related factors of unintentional injuries at school. Results: About 22.9 percent of the adolescents had treatment experiences for unintentional injuries at school in the past 12 months. At the individual level, the significant factors associated with unintentional injuries at school included gender, grade, academic achievement, current smoking, alcohol consumption, frequency of high caffeine intake, depression, and relief of fatigue after sleep. At the group level, the significant factors included number of physical education per week and safety education. Conclusion: School based injury prevention programs should be sensitive to both individual- and school-level factors associated with unintentional injury at school among Korean adolescents.

Antidiabetic Coumarin and Cyclitol Compounds from Peucedanum japonicum

  • Lee, Sung-Ok;Choi, Sang-Zin;Lee, Jong-Hwa;Chung, Sung-Hyun;Park, Sang-Hyun;Kang, Hee-Chol;Yang, Eun-Young;Cho, Hi-Jae;Lee , Kang-Ro
    • Archives of Pharmacal Research
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    • 제27권12호
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    • pp.1207-1210
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    • 2004
  • The antidiabetic activity-guided fractionation and isolation of the 80% EtOH extracts from Peucedani Radix (Peucedanum japonicum, Umbelliferae) led to the isolation and characterization of a coumarin and a cyclitol as active principles, that is, peucedanol 7-O-${\beta}$ -D-glucopyranoside (1) and myo-inositol (2). Their structures were identified by spectroscopic methods. Compound 1 showed 39% inhibition of postprandial hyperglycemia at 5.8 mg/kg dose, and compound 2 also significantly inhibited postprandial hyperglycemia by 34% (P<0.05).

고차 고조파 전류의 보상을 위한 단상 능동전력필터 (Single-Phase Active Power Filter for Higher-order Harmonic Current Compensation)

  • 성기석;우명호;송중호;최익;임묘택
    • 대한전기학회논문지:전기기기및에너지변환시스템부문B
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    • 제49권7호
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    • pp.500-508
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    • 2000
  • Basic design for single-phase active power filter, which aims at railway application provided with PWM-controlled converters, is comprehensively studied and its performance is presented in this paper. Active power filters are used to compensate railway signaling and public telecommunication interference due to the high-order harmonic currents generated in railway traction locomotives. A type of hybrid digital filter which is composed of low pass filter and high pass filter is proposed so that the desired harmonic reference current with accurate magnitude and phase shift can be extracted from catenary line current. A design criteria to determine input inductor L and output capacitor C is also described, considering voltage, current, PWM pattern, and switching frequency of the main converters. Finally, computer simulation and DSP-based experiments resulted from laboratory test are presented.

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