• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.101-111
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• 2017

The task of improving a fungal strain is highly time-consuming due to the requirement of a large number of flasks in order to obtain a library with enough diversity. In addition, fermentations (particularly those for fungal cells) are typically performed in high-volume (100-250 ml) shake-flasks. In this study, for large and rapid screening of itaconic acid (IA) high-yielding mutants of Aspergillus terreus, a miniaturized culture method was developed using 12-well and 24-well microtiter plates (MTPs, working volume = 1-2 ml). These miniaturized MTP fermentations were successful, only when highly filamentous forms were induced in the growth cultures. Under these conditions, loose-pelleted morphologies of optimum sizes (less than 0.5 mm in diameter) were casually induced in the MTP production cultures, which turned out to be the prerequisite for the active IA biosynthesis by the mutated strains in the miniaturized fermentations. Another crucial factor for successful MTP fermentation was to supply an optimal amount of dissolved oxygen into the fermentation broth through increasing the agitation speed (240 rpm) and reducing the working volume (1 ml) of each 24-well microtiter plate. Notably, almost identical fermentation physiologies resulted in the 250 ml shake-flasks, as well as in the 12-well and 24-well MTP cultures conducted under the respective optimum conditions, as expressed in terms of the distribution of IA productivity of each mutant. These results reveal that MTP cultures could be considered as viable alternatives for the labor-intensive shake-flask fermentations even for filamentous fungal cells, leading to the rapid development of IA high-yield mutant strains.

• The Journal of Korean Physical Therapy
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• 제28권6호
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• pp.381-384
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• 2016

Purpose: Previous studies reported changes in the first metatarsophalangeal (MTP) joint angle in relation with and without weight bearing, but it is unclear whether sesamoid bone of the great toe changes in weight bearing conditions particularly in subjects with hallux valgus (HV). To investigate how weight bearing conditions can affect the position of the medial sesamoid bone (MSB), first MTP joint angle, and second intermetatarsal angle (IMA) in the recruited subjects. Methods: Subjects were recruited 24 with HV and 21 without HV in study. X-rays were taken in the weight bearing and non-weight bearing conditions. The distance of the MSB, first MTP joint angle, and second IMA were measured from the radiographs. Data were analyzed by paired and Independent t-test. The statistical significance level was p<0.05. Results: In both groups, the first MTP joint angles and the distance of the MSB were significantly smaller, while the second IMA was significantly greater in the weight bearing condition. The difference in the distance of the MSB between the two postures was significantly greater in the group with HV. Conclusion: Weight bearing can affect the first MTP joint angle, second IMA, and position of the MSB; the change in the position of the MSB in weight bearing was greater in the group with hallux valgus. The difference in these variables between weight bearing and non-weight bearing conditions may be considered when measuring HV.

• 한국염색가공학회지
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• 제6권4호
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• pp.40-45
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• 1994

To improve the blood compatibility of cellulose membrane, 2-(methacryloyloxy)ethyl-2-(trimethylammonium)ethyl phosphate(MTP), which is a methacrylate with phospholipid polar group, and glycidyl methacrylate(GMA) were grafted simultaneously on the surface of membrane and the biocompatibility of grafted membrane was investigated. There was no difference of permeability between the MTP and GMA-grafted and the original cellulose membrane. The permeation pathway for a solute whose molecular weight was above 10$^{4}$ is maintained after grafting on the surface of membrane. The cellulose membrane grafted with MTP and GMA effectively suppressed thrombogenicity for the rabbit blood. This effect became more clear with increasing the surface distribution of phospholipid polar groups.

• 대한족부족관절학회지
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• 제19권3호
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• pp.128-131
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• 2015

Capsuloligamentous injury at the first metatarsophalangeal (MTP) joint is a common traumatic injury during physical activity, particularly on artificial turf. Mechanism of injury include excessive flexion, extension, or valgus stress. We report a non-operatively treated capsuloligamentous injury at the first MTP joint, which did not occur traumatically but developed by a stress-related mechanism in a collegiate rower.

• 한국막학회:학술대회논문집
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• 한국막학회 1992년도 추계 총회 및 학술발표회
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• pp.47-48
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• 1992

Polymer containing monomers with pendant phospholipid polar group, 2-(metha-cryloyoxy) ethyl-2-(trimethylammonium) ethyl phosphate(MTP) were synthesized and blood compatibility of the copolymers was evaluated. Good permeability of biocomponents of molecular weight below 10$^4$ through cellulosic membrane coated with the copolymer of 2-(methacryloyoxy) ethyl-2-(tri-methylammonium)ethyl phosphate, butylmethacrylate(BMA), and glycidyl methacrylate(GMA) was observed, (Fig.1).

• 대한족부족관절학회지
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• 제11권2호
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• pp.171-176
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• 2007

Purpose: We assess the mid to long term follow up results of arthrodesis of the first metatarso-phalangeal (MTP) joint and resection arthroplasty of the lesser toes in rheumatoid arthritic forefoot deformity. Materials and Methods: Between 1998 to 2001 year, 25 cases (18 patients) rheumatoid forefoot deformities were surgically corrected. Follow up period was 83 months (range, 63 to 90 months). The clinical outcome was evaluated using subjective satisfaction and AOFAS score. The radiological measurements were hallux valgus angle, first and second intermetatarsal angle, second metatarso-phalangeal angle (MTP-$2^{nd}$ angle). Results: Subjective satisfaction was 76%. AOFAS score improved from 37 to 73. The hallux valgus angle improved from preoperative $39^{\circ}$ ($27{\sim}64^{\circ}$) to $14^{\circ}$ ($4{\sim}34$) at the last follow up. The intermetatarsal angle were preoperative $13^{\circ}$ ($6{\sim}22^{\circ}$) to $11^{\circ}$ ($3{\sim}13^{\circ}$) at the last follow up, The MTP-$2^{nd}$ angle were preoperative $24^{\circ}$ ($9{\sim}47$) to last follow up $15^{\circ}$ ($2{\sim}39^{\circ}$) respectively (p>0.05). Complication was intractable callus 10 cases, Interphalangeal arthritis 5 cases. Conclusion: Mid to long term outcomes rheumatoid forefoot reconstruction by first MTP arthrodesis and resection arthroplasty of lesser toes results a satisfaction and pain relief.

• 한국통신학회논문지
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• 제23권5호
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• pp.1397-1408
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• 1998

미래의 통신 망을 위한 서비스 망들은 ATM을 기반으로 하는 B-ISDN 망으로 통합될 것이며 이러한 서버스 망들은 사용자 요구 서비스를 제어하기 위한 신호 전달 망으로 공통선 선호 방식을 사용하게 된다. 이에 따라, ITU-T 에서는 기존의 N-ISDN 공통선 신호의 MTP 신호 계층을 대신하여 B-ISDN 선호 계층들이 권고되었다. 본 논문 에서는 B-ISDN 공중망의 ATM 교환 시스탬에 구현된 공통선 선호 기능에 대해 각 선호 계층별 특성, 기능 및 실현 구조등을 제안하고 성능을 분석하였다. SAAL 계층은 linked-list와 단위 프레임 길이를 사용하는 SSCOP 송수신 버퍼 구조를 제안하고 성능을 분석하였으며, MTP-3b 계층에 대해서도 ATM 교환 시스템 구조에 따른 실현 구조 및 내부 라우팅 방법을 제안하고 그 성능을 분석하였다. 또한, B-ISDN 도입 초기에 나타나는 기존의 N-ISDN 망과 B-ISDN의 SS No.7 연동에 대한 효율적인 연동 구조로서 회선 관련 신호망은 대응 모드만을 사용하는 ISUP/B-ISUP 레벨 연동을 제안하였다.

• 한국통신학회논문지
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• 제22권6호
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• pp.1343-1355
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• 1997

Full motion video data is the most effective data type that transmits information in multimedia enviornment. VOD service is system that uses network to transmit full motion video data interactively. Therefore VOD service system must satisfy the following requirements. First, it should transmit high quality full motion video. Second, it should be able to control a large quantity of full motion video. Third, a user should be able to retrieve the full motion vide effectively. However the existing VOD service system is established based n file system that not only does it have disadvantages in managment such as transaction control but also it is not suitable for distributed multimedia environment. The goal of this research is to build a VOD service system that provides high-quality full motion video assuming information retrieval. In order to mainly search video data's bibliographical information and to manage related information, VOD service system and DBMS system are to be closely related. Also, to transmit high quality full motion video data, we use MTP$^{TM}$(Media Transfer Protocol) developed by StarLight. MTP$^{TM}$ guarantees high bandwidth that is necessary to tansmit high quality full motion video data effectively. In other hand TCP/IP which is used for information search does not guarentee high bandwidth as that in MTP$^{TM}$. Therfore VOD service system that seraches information managed by DBMS and transmits requried full motion video data should control different kinds of protocol simultaneously. This paper describes the configuration of VODB system that has solved these problems, implementation and efficiency of data applied system.stem.

• Archives of Pharmacal Research
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• 제28권7호
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• pp.810-815
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• 2005

Previously, we have shown that astrocytes deprived of glucose became highly vulnerable to peroxynitrite, and adenosine and its metabolites attenuated the gliotoxicity via the preservation of cellular ATP level. Here, we found that adenosine and related metabolites prevented the disruption of mitochondrial transmembrane potential (MTP) in glucose-deprived rat primary astrocytes exposed to 3-morpholinosydnonimine (SIN-1), a peroxynitrite releasing agent. Exposure to glucose deprivation and SIN-1(2h) significantly disrupted MTP in astrocytes, and adenosine prevented it in dose-dependent manner with an $EC_{50}\;of\;5.08{\mu}M$. Adenosine also partially prevented the cell death by myxothiazol, a well-known inhibitor of mitochondrial respiration. Blockade of adenosine deamination or intracellular transport with erythro-9-(-hydroxy-3-nonyl)adenosine (EHNA) or S-(4-nitrobenzyl)-6-thioinosine (NBTI), respectively, completely reversed the protective effect of adenosine. Other purine nucleos(t)ides including inosine, guanosine, ATP, ADP, AMP, ITP, and GTP also showed similar protective effects. This study indicates that adenosine and related purine nucleos(t)ides may protect astrocytes from peroxynitrite-induced mitochondrial dysfunction.

• 생명과학회지
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• 제24권7호
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• pp.743-749
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• 2014

Orotic acid 1% 수준으로 지방간을 유발시킨 흰쥐에 Monascus purpureus 균주 발효 당귀 5%를 식이 첨가하여 간 조직 및 혈중의 지질대사에 미치는 영향을 검토하였다. 발효 참당귀 분말을 10일간 식이 급여한 결과, orotic acid 유발 지방간 흰쥐의 간 조직 내 중성지질 농도를 낮추고, 혈청 지질 농도가 정상 수준으로 회복되었음을 확인하였다. 간 조직 내 MTP mRNA 및 PDI mRNA 발현 정도는 정상군에 비해 발효 당귀군에서 약간 증가하는 경향을 보이거나, 유의적으로 증가된 수치를 나타내었다. 간 조직의 형태학적 관찰에서는 세포 간극이 좁고 잘 구성된 소엽구조를 하고 있는 정상군에 반해 OA 대조군은 orotic acid 투여로 소포성 지방 변성이 소엽 중심대에 나타나 있고, 지방세포의 크기와 수가 증가하여 전형적인 지방간 형태의 양상을 보였다. 한편, 발효 당귀군의 간장은 간세포의 소포성 지방세포들이 현저히 감소함으로써, 정상 수준의 형태학적 간 성상을 나타내어 지방간 개선에 긍정적인 영향을 미치는 것으로 판단되며, 간 조직의 지질 축적 억제로 인한 지질대사 개선에도 효과가 있는 것으로 사료된다.