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Development and Application of Learning on Geological Field Trip Utilizing on Social Construction of Scientific Model (과학적 모델의 사회적 구성을 활용한 야외지질학습 개발 및 적용)

  • Choi, Yoon-Sung;Kim, Chan-Jong;Choe, Seung-Urn
    • Journal of the Korean earth science society
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    • v.39 no.2
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    • pp.178-192
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    • 2018
  • The purposes of this study were to develop and apply on learning on geological field trip utilizing the social construction of scientific model. We developed field trip places by considering not only Orion (1993)'s novelty space but also the achievement standards of 2015 national curriculum. The subjects of the study were 8 in the 'G' science gifted education center. We conducted a study using the theme of 'How was formed Mt. Gwanak?' on 5 lessons including a series of 2 field trip lessons and 3 lessons utilizing the social construction of scientific model. Students participated in pre- and post-test on the understanding of scientific knowledge about formation of mountain. Semi-structured interview was used to analyze students' learning about geological field trip in terms of affective domain. Results were as follows. First, there were 2 places of upper-stream valley and down-stream valley separately. They contained outcrops gneiss, granite, joint in the valley, xenolith, fault plane, mineral in the valley. Second, pre- and post-test and semi-structure interview were analyzed in terms of what scientific knowledge students learned about and how Mt. Gwanak was formed. Seven students explained that Mt. Gwanak was volcano during pretest. Seven students described how granite was formed to form Mt. Gwanak. They also understood geological time scale, i.e., metamorphic rock. Third, the geological field trip was effective to low achievement geoscience students as they engaged in the activities of field trip. Using positive responses on affective learning was effective on learning on geological field trip when utilizing the social construction of scientific model. This study suggests that teachers use an example 'model' on geoscience education. This study also suggests that teachers apply the social construction of scientific model to geological field trip.

Molecular Characterization of AceB, a Gene Encoding Malate Synthase in Corynebacterium glutamicum

  • Lee, Heung-Shick;Anthony J. Sinskey
    • Journal of Microbiology and Biotechnology
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    • v.4 no.4
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    • pp.256-263
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    • 1994
  • The aceB gene, encoding for malate synthase, one of the key enzymes of glyoxylate bypass, was isolated from a pMT1-based Corynebacterium glutamicum gene library via complementation of an Escherichia coli aceB mutant on an acetate minimal medium. The aceB gene was closely linked to aceA, separated by 598 base pairs, and transcribed in divergent direction. The aceB expressed a protein product of Mr 83, 000 in Corynebacterium glutamicum which was unusually large compared with those of other malate synthases. A DNA-sequence analysis of the cloned DNA identified an open-reading frame of 2, 217 base pairs which encodes a protein with the molecular weight of 82, 311 comprising 739 aminoo acids. The putative protein product showed only limited amino acid-sequence homology to its counteliparts in other organisms. The N-terminal region of the protein, which shows no apparent homology with the known sequences of other malate synthases, appeared to be responsible for the protein s unusually large size. A potential calciumbinding domain of EF-hand structure found among eukaryotes was detected in the N-terminal region of the deduced protein.

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Determination of the Optimal Strategy for Pump-And-Treat Method

  • Ko, Nak-Youl;Lee, Kang-Kun
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2001.09a
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    • pp.204-207
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    • 2001
  • An optimization process for the design of groundwater remediation is developed by simultaneously considering the well location and the pumping rate. This process uses two independent models: simulation and optimization model. Groundwater flow and contaminant transport are simulated with MODFLOW and MT3D in simulation model. In optimization model, the location and pumping rate of each well are determined and evaluated by the genetic algorithm. In a homogeneous and symmetric domain, the developed model is tested using sequential pairs for pumping rate of each well, and the model gives more improved result than the model using sequential pairs. In application cases, the suggested optimal design shows that the main location of wells is on the centerline of contaminate distribution. The resulting optimal design also shows that the well with maximum pumping rate is replaced with the further one from the contaminant source along flow direction and that the optimal pumping rate declines when more cleanup time is given. But the optimal pumping rate is not linearly proportional to the cleanup time and the minimum total pumping volume does not coincide with the optimal pumping rate.

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Analysis of Slope Stability by Using Remote Sensing and GIS Around Chungju Area (원격탐사와 지구정보시스템을 이용한 충주지역의 사면안정분석)

  • Shin, Hyunjun;Lee, Younghoon;Min, Kyungduck;Won, Joongsun;Kim, Younjong
    • Economic and Environmental Geology
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    • v.29 no.5
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    • pp.615-622
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    • 1996
  • Slope stability analysis was conducted using remote sensing and Geoscientific Information System (GIS) as a part of natural hazard assessment around Chungju area. Landsat TM band 5 and 7 which contain more information about geological structure and geography are chosen and processed to analyse regional geological structure. Through image processing technique such as PCA, HFF, edge detection and enhancement, regional lineament can be mapped and identified. The lineament density map is constructed based on summed length of lineaments per unit area and the study area can be divided into 7 structural domains. Various factors of slope stability analysis such as geology, slope aspect, degree of slope, landcover, water shed as well as characterized structural domain are constructed as a database of GIS. Rating and weighting of each factor for slope stability analysis is decided by considering environmental geological characteristics of study area. Spatial analysis of regional slope stability is examined through overlaying technique of the GIS. The result of areal distribution of slope stability shows that the most unstable area is all over Jaeogae-ni, Hyangsan-ni and Mt. Daedun.

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Investigation of the effect of SRSF9 overexpression on HIV-1 production

  • Ga-Na, Kim;Kyung-Lee, Yu;Hae-In, Kim;Ji Chang, You
    • BMB Reports
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    • v.55 no.12
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    • pp.639-644
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    • 2022
  • Serine-arginine-rich splicing factors (SRSFs) are members of RNA processing proteins in the serine-arginine-rich (SR) family that could regulate the alternative splicing of the human immunodeficiency virus-1 (HIV-1). Whether SRSF9 has any effect on HIV-1 regulation requires elucidation. Here, we report for the first time the effects and mechanisms of SRSF9 on HIV-1 regulation. The overexpression of SRSF9 inhibits viral production and infectivity in both HEK293T and MT-4 cells. Deletion analysis of SRSF9 determined that the RNA regulation motif domain of SRSF9 is important for anti-HIV-1 effects. Furthermore, overexpression of SRSF9 increases multiple spliced forms of viral mRNA, such as Vpr mRNA. These data suggest that SRSF9 overexpression inhibits HIV-1 production by inducing the imbalanced HIV-1 mRNA splicing that could be exploited further for a novel HIV-1 therapeutic molecule.

Numerical Simulation on the Wind Ventilation Lane and Air Pollutants Transport due to Local Circulation Winds in Daegu Districts (대구지역의 국지순환풍의 환기경로 및 대기오염수송에 관한 수치모의)

  • Koo, Hyun-Suk;Kim, Hae-Dong
    • Journal of the Korean earth science society
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    • v.25 no.6
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    • pp.418-427
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    • 2004
  • Recently, urban planning with consideration of urban climate, represented by the concept of urban ventilation lane is widely practiced in many countries. The concept of urban ventilation lane is mainly aimed to improve the thermal comfort within urban area in summer season. It has also the aim to reduce the urban air pollution by natural cold air drainage flows which are to be intensified by a suitable alignment of buildings as well as use zonings based on scientific reasons. In this study, the prevailing wind ventilation lane of a local wind circulation and around Daegu for a typical summer days was investigated by using a numerical simulation. The transport of air pollutants by the local circulation winds was also investigated by using the numerical simulation model, the RAMS (Reasonal Atmospheric Model System).The domain of interest is the vicinity of Daegu metropolitan city (about 900 km2). The horizontal scale of the area is about 30 km. The simulations were conducted under a late spring synoptic condition with weak gradient wind and almost clear sky. From the numerical experiment, the following three conclusions were obtained: (1) The major wind passages of the local circulation wind generated by radiative cooling over the representative mountains of Daegu (Mt. Palgong and Mt. Ap) were found. The winds blow down along the valley axis over the eastern part of Daegu as a gravity flow during nighttime. (2) At the flatland, the winds blow toward the western part of Daegu through the city center. (3) As the results, the air pollutants were transported toward the western part of Daegu by the winds during nighttime.

Soil Water Monitoring in Below-Ground Ectomycorrhizal Colony of Tricholoma Matsutake

  • Koo, Chang-Duck;Kim, Je-Su;Lee, Sang-Hee;Park, Jae-In;Kwang- Tae Ahn
    • The Korean Journal of Quaternary Research
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    • v.17 no.2
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    • pp.129-133
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    • 2003
  • Water is critically important for Tricholoma matsutake(Tm) growth because it is the major component of the mushroom by over 90%. The mushroom absorbs water through the below ground hyphal colony. Therefore, the objectives of our study were to investigate spatio-temporal water changes in Tm colonies. This study was carried out at Tm fruiting sites in Sogni Mt National Park, where the below-ground mushroom colonies have been irrigated. To identify spatial water status within the Tm soil colony soil moisture and ergosterol content were measured at six positions including a mushroom fruiting position on the line of the colony radius. To investigate temporal soil moisture changes in the soil colony, Time Domain Reflectometry(TDR) sensors were established at the non-colony and colony front edge, and water data were recorded with CR10X data logger from late August to late October. Before irrigation, whereas it was 12.8% at non-colony, the soil water content within Tm colony was 8.0% at 0-5cm from the colony front edge, 6.2% at 10-15cm and 6.5-7.5% at 20-40cm. And the content was 12.1% at 80cm distance from the colony edge, which is similar to that at the non-colony. In contrast, ergosterol content which is proportional to the live hyphal biomass was only 0.4${\mu}g$/g fresh soil at the uncolonized soil, while 4.9 $\mu\textrm{g}$/g fresh soil at the front edge where the hyphae actively grow, and 3.8 ${\mu}g$/g fresh soil at the fruiting position, l.1${\mu}g$/g at 20cm distance and 0.4${\mu}g$/g in the 40cm rear area. Generally, in the Tm fungal colony the water content changes were reversed to the ergosterol content changes. While the site was watered during August to October, the soil water contents were 13.5∼23.0% within the fungal colony, whereas it was 14.5∼26.0% at the non-colony. That is, soil water content in the colony was lower by 1.0∼3.0% than that in the non-colonized soil. Our results show that Tm colony consumes more soil water than other parts. Especially the front 30cm within the hyphal colony parts is more critical for soil water absorption.

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Studies on the Assumption of the Locations and Formational Characteristics in Yigye-gugok, Mt. Bukhansan (북한산 이계구곡(耳溪九曲)의 위치비정과 집경(集景) 특성)

  • Jung, Woo-Jin;Rho, Jae-Hyun;Lee, Hee-Young
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.35 no.3
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    • pp.41-66
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    • 2017
  • The purpose of this research is to empirically trace the junctures of Yigye-gugok managed by Gwan-am Hong Gyeong-mo, a grandson of Yigye Hong Yang-ho who originally designed Yigye-gugok, while reviewing the features of the forms and patterns of gugok. The results of the research are as follows. 1. Ui-dong was part of the domain of the capital during the Chosun dynasty, which also is located in the city of Seoul as a matter of administrative zone. Likewisely, Yigye-gugok is taken as a special meaning for it was one and only gugok. Starting with Mangyeong Waterfall as the $1^{st}$ gok, Yigye follows through the $2^{nd}$ gok of Jeokchwibyeong Rock, the $3^{rd}$ gok of Chanunbong Peak, the $4^{th}$ gok of Jinuigang Rock, the $5^{th}$ gok of Okkyeongdae Rock, the $6^{th}$ gok of Wolyeongdam Pond, the $7^{th}$ gok of Tagyeongam Rock, the $8^{th}$ gok of Myeongoktan Stream, and the $9^{th}$ gok of Jaeganjeong Pavilion. Of these, Mangyeong Waterfall, Chanunbong Peak, and Okkyeongdae Rock are distinct for their locations in as much as their features, while estimated locations for Jinuigang Rock, Wolyeongdam Pond, Myeongoktan Stream, and Jaeganjeong Pavilion were discovered. However, Jeokchwibyeong Rock and Tagyeongam Rock demonstrated multiple locations in close resemblance to documentary literatures within secretive proximity, whereas geography, scenery, and sighted objects were considered to evaluate the 1st estimated location. Through these endeavored, it was possible to identify the shipping routes and structures for the total distance of 2.1km running from the $1^{st}$ gok to the $9^{th}$ gok, which nears Gwanam's description of 5ri(里), or approximately 1.96km for gugok. 2. Set towards the end of the $18^{th}$ century, Yigye-gugok originated from a series of work shaping the space of Hong Yang-ho's tomb into a space for the family. Comparing Yigye-gugok to other gugoks, numerous differences are apparent from beyond the rather more general format such as adjoining the $8^{th}$ gok while paving through the lower directions from the upper directions of the water. This gives rises to the interpretation such that Yigye-gugok was positioned to separate the doman of the family from those of the other families in power, thereby taking over Ui-dong. Yet, the aspect of the possession of the space lends itself to the determination that the location positioned at the $8^{th}$ gok above Mangyeongpok Waterfall representing Wooyi-dong was a consequence of the centrifugal space creation efforts. 3. While writings and poetic works were manufactured in such large quantities in Yigye-gugok whose products of setters and managers seemed intended towards gugok-do and letters carved on the rocks among others, there is yet a tremendous lack of visual media in the same respect. 'Yigye-gugok Daejacheop' Specimens of Handwriting offers the traces of Gwanam's attempts to engrave gakja at the food of Yigye-gugok. This research was able to ascertain that 'Yigye-gugok Daejacheop' Specimens of Handwriting was a product of Hong Yang-ho's collections maintained under the auspices of the National Central Museum, which are renowned for Song Shi-yeol's penmanship.