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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Investigation of the effect of SRSF9 overexpression on HIV-1 production

  • Ga-Na, Kim (Department of Pathology, National Research Laboratory for Molecular Virology, College of Medicine, The Catholic University of Korea) ;
  • Kyung-Lee, Yu (Department of Pathology, National Research Laboratory for Molecular Virology, College of Medicine, The Catholic University of Korea) ;
  • Hae-In, Kim (Department of Pathology, National Research Laboratory for Molecular Virology, College of Medicine, The Catholic University of Korea) ;
  • Ji Chang, You (Department of Pathology, National Research Laboratory for Molecular Virology, College of Medicine, The Catholic University of Korea)
  • Received : 2022.10.20
  • Accepted : 2022.11.02
  • Published : 2022.12.31
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Abstract

Serine-arginine-rich splicing factors (SRSFs) are members of RNA processing proteins in the serine-arginine-rich (SR) family that could regulate the alternative splicing of the human immunodeficiency virus-1 (HIV-1). Whether SRSF9 has any effect on HIV-1 regulation requires elucidation. Here, we report for the first time the effects and mechanisms of SRSF9 on HIV-1 regulation. The overexpression of SRSF9 inhibits viral production and infectivity in both HEK293T and MT-4 cells. Deletion analysis of SRSF9 determined that the RNA regulation motif domain of SRSF9 is important for anti-HIV-1 effects. Furthermore, overexpression of SRSF9 increases multiple spliced forms of viral mRNA, such as Vpr mRNA. These data suggest that SRSF9 overexpression inhibits HIV-1 production by inducing the imbalanced HIV-1 mRNA splicing that could be exploited further for a novel HIV-1 therapeutic molecule.

Keywords

Acknowledgement

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (2020R1F1A1075725, 2017R1A5A1015366 and 2020R1I1A1A01073574).

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