• Title/Summary/Keyword: MS0

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Plant Regeneration from Cotyledon and Hypocotyl Culture in Apple Hybrid Seedling (P.16 $\times$ Malus prunifolia) (사과 P.16 $\times$ Malus prunifolia 교잡실생의 자엽 및 배축배양에 의한 식물체 재분화)

  • 김송남;오성도;김영숙
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.191-195
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    • 2000
  • Adventitious shoots were induced from cotyledon and hypocotyl explants of apple hybrid seedlings (P.16$\times$ Malus prunifolia) on MS medium supplement with 2,4-D and various cytokinine (Kn. BA, TDZ) The shoot regeneration from the cotyledon culture was the highest on MS medium supplemented with 1.0 mg/L NAA and 2.0 mg/L BA. Whereas in case of hypocotyl culture, it was the highest on MS medium supplemented with 0.5 mg/L NAA and 0.5 mg/L BA. However, in the MS medium without BA there was no shoot regeneration. Hypocotyl culture seemed to be more effective than cotyledon culture in shoot regeneration. Specially, the top position of the hypocotyl found to be the best explant for shoot induction among the other segments of hypocotyls. Regenerated shoots were rooted on half-stength MS medium with 1.0 mg/L NAA. Above results suggest that Apple hybrid (P.16 $\times$ Malus prunifolia) can be multiplied via cotyledon or hypocotyl culture systems.

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Callus Induction from Seeds of Italian ryegrass and Plant Regeneration (이탈리안 라이그라스 종자로부터 캘러스 유도 및 식물체 재분화)

  • 임용우;김기용;최기준;성병렬;신정섭
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.20 no.1
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    • pp.25-30
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    • 2000
  • The conditions for callus formation and plant regeneration were confirmed in Italian ryegrass (Lolium mulfiorum Lam.). Among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu) MS medium was highest degree of efficiencies respectively in callus formation and plant regeneration. In this study, we determined volume of hormones and other compounds appended in media. For callus formation, only $5\;mg/\;{\ell}$ of 2,4-D (2,4-dichlorophenoxy acetic acid) was appended in their media. For plant regeneration, we used MS medium containing $1.0\;mg/\;{\ell}$ of BA and $0.1\;mg/\;{\ell}$ of NAA.

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Development of Simultaneous Analytical Method for Five Lignans in Edible Seeds (종자류 식품에 함유된 5종 리그난의 동시 분석법 개발)

  • Yoonjeong, Kim;Jiye, Pyeon;In-hwan, Baek;Younghwa, Kim
    • The Korean Journal of Food And Nutrition
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    • v.35 no.6
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    • pp.543-551
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    • 2022
  • There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R2≥0.99). The limits of detection were 0.040~0.765 ㎍/100 g and the limits of quantification were 0.114~1.532 ㎍/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 ㎍/100 g, 78.965 ㎍/100 g, and 165.521 ㎍/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.

Sensitive Determination of Felodipine in Human Plasma by LC-MS/MS

  • Kim, Ho-Hyun;Roh, Hyeon-Jin;Lee, He-Joo;Han, Sang-Beom
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.283.2-283.2
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    • 2003
  • This study established a highly sensitive novel quantification method for detecting felodipine in human plasma using LC-MS/MS. The mobile phase used after degassing was composed of 1 mM ammonium acetate and acetonitrile (20:80, pH 6.0), with flow rate of 200 uL/min. One mL plasma were pipetted into glass tubes and spiked with 0.1 mL of internal standard solution. (omitted)

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Determination of Glimepiride in Human Plasma by LC-MS/MS

  • Kim, Ho-Hyun;Roh, Hyeong-Jin;Lee, Hee-Joo;Han, Sang-Beom
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.282.1-282.1
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    • 2003
  • This study established a sensitive novel Quantification method for detecting glimepiride in human plasma using LC-MS/MS for pharmacokinetic studies. The mobile phase used after degassing was composed of 10 mM ammonium acetate and acetonitrile (20:80, pH 3.0), with flow rate of 200uL/min. One mL plasma were pipetted into glass tubes and spiked with 0.1 mL of internal standard solution. (omitted)

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Lifestyle Characteristics, Metabolic Syndrome Risk Factors and Risk of Cardiovascular Disease among Workers in Kyeongki-do (경기도 지역 대사증후군 근로자의 생활습관특성, 대사증후군 위험요인 및 심혈관 위험도)

  • Kim, Chun-Ja;Park, Jae-Bom;Kim, Bom Taek
    • Korean Journal of Occupational Health Nursing
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    • v.17 no.2
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    • pp.230-238
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    • 2008
  • Purpose: This study was to investigate the lifestyle characteristics and risk of cardiovascular disease (CVD) among workers with metabolic syndrome(MS). Methods: The descriptive cross-sectional survey design was used. A total of 187 workers with MS were recruited from a university hospital. The anthropometric measures were used and blood data were reviewed from the health record. Data were analyzed using descriptive statistics and multiple logistic regressions with SPSS/WIN 12.0. Results: While 52.2% of women did not do exercise regularly, 30.5% of men did not at all. The prevalence of MS according to each component was as follows: hypertension was 92.0%, obesity was 89.8%, hyperlipidemia was 63.1%, and hyperglycemia was 61.0%. The high prevalence of each component of MS in workers with MS indicated a significantly higher risk of CVD. The odds ratio of risk of CVD was 16.04 (p=.017) in workers with hypertension, when compared to workers without hypertension. Similarly, the odds ratio of risk of CVD was 11.04 (p=.000) in workers with hyperglycemia, compared to workers without hyperglycemia. Conclusion: Increased risk of CVD was significantly associated with lifestyle characteristics and MS risk factors among Korean workers with MS.

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A Study on Implementation of Powerline Carrier Modem using 5-ary Frequency Shift Keying Method (5-ary 주파수 천이 키잉 방식을 이용한 전력선 캐리어 모뎀 구현에 관한 연구)

  • Park, Sung-Wook;Park, Jong-Wook
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.42 no.3
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    • pp.39-44
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    • 2005
  • In this paper, we use the 5-ary frequency shift keying method for robust powerline communication. To transmit the data by the 3-ary carrires among 5-ary carrirers and three carriers are 115kHz, 120kHz and 125kHz. Each time of three carrires is 0.33ms and total transmitting time duration from zero crossing point has 1ms. We use the dummy carriers which are 50kHz and 350kHz for monitoring the powerline channel state, dummy signal duration is received during 1ms. Experimentation result is shown that the implemented PLC transceiver has better than chirp transceiver at the capacitive load testing in spite of low transmission power.

The Antioxidant Activity of Various Cultivars of Grape Skin Extract

  • Yoo, Mi-Ae;Kim, Jin-Sook;Chung, Hae-Kyung;Park, Won-Jong;Kang, Myung-Hwa
    • Food Science and Biotechnology
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    • v.16 no.6
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    • pp.884-888
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    • 2007
  • The aim of this study was to analyze the antioxidant properties of different cultivars of grape skin extract in an in vitro system. The extracts were prepared from eight grape cultivars: 'Campbell Early' (CE), 'Kyoho' (K), 'New Kyoho' (NK), 'Muscat of Alexandria' (MOA), 'Seibel' (S), 'Morgen Schow' (MS), 'Gold Finger' (GF), and 'Meru' (M). The total phenolic acid contents were highest in MS and K. Resveratrol content was high in NK (50.88 mg/l g of coat), and quercetin content was significantly higher in K (0.68 mg/l g of coat) than in the other grape species (0.21-0.44 mg/l g of coat). The K and MS grape species, in which total phenol content was comparatively high (K: $24.15\;{\mu}g/mL$, MS: $25.52\;{\mu}g/mL$), also showed a high level of electron donating activity (K, 53%; MS, 59%). The hydrogen radical scavenging activity of M (50.36%) was significantly higher than the other grape species, including the S (50.21%), MS (49.43%), and K (49.06%) cultivars. Antioxidant activity varied depending on grape species, but overall it was highest in the MS and K cultivars.

A 10b 100MS/s 27.2mW $0.8mm^2$ 0.18um CMOS Pipeline ADC with Various Circuit Sharing Schemes (다양한 회로 공유기법을 사용하는 10비트 100MS/s 27.2mW $0.8mm^2$ 0.18um CMOS Pipeline ADC)

  • Yoon, Kun-Yong;Lee, Se-Won;Choi, Min-Ho;Lee, Seung-Hoon
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.46 no.4
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    • pp.53-63
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    • 2009
  • This work proposes a 10b 100MS/s 27.2mW $0.8mm^2$ 0.18um CMOS ADC for WLAN such as an IEEE 802.11n standard. The proposed ADC employs a three-stage pipeline architecture and minimizes power consumption and chip area by sharing as many circuits as possible. Two multiplying DACs share a single amplifier without MOS switches connected in series while the shared amplifier does not show a conventional memory effect. All three flash ADCs use only one resistor ladder while the second and third flash ADCs share all pre-amps to further reduce power consumption and chip area. The interpolation circuit employed in the flash ADCs halves the required number of pre-amps and an input-output isolated dynamic latch reduces the increased kickback noise caused by the pre-amp sharing. The prototype ADC implemented in a 0.18um n-well 1P6M CMOS process shows the DNL and INL within 0.83LSB and 1.52LSB at 10b, respectively. The ADC measures an SNDR of 52.1dB and an SFDR of 67.6dB at a sampling rate of 100MS/s. The ADC with an active die area of $0.8mm^2$ consumes 27.2mW at 1.8V and 100MS/s.

In Vitro Plantlet Regeneration from Axillary Buds of Tilia amurensis Mature Trees and Clonal Variation in Tissue Culturability (피나무 성숙목(成熟木)의 액아배양(腋芽培養)에 의한 유식물체(幼植物體) 재생(再生)과 조직배양능력(組織培養能力)에 있어서의 클론간(間) 변이(變異))

  • Youn, Yang;Ohba, Kihachiro
    • Journal of Korean Society of Forest Science
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    • v.79 no.2
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    • pp.109-114
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    • 1990
  • The axillary buds of 15-year-old Tilia amurensis were cultured on Saito and Ide (IS), Murashige and Skoog (MS) media and woody plant medium (WPM) to establish an effective micropropagation method. Five levels of 6-benzylaminopurine (BAP) were tested. On IS medium and WPM addition of 1.0/l BAP enhanced shoot development and shoot elongation, whereas addition of 0.5/l BAP was effective on MS medium. A better results were obtained from WPM with 1.0/l BAP and MS with 0.1/l BAP. Developed shoots were subcultured on each basal media but with 0.2/l BAP, Multiple shoots were almost doubled in a month. Root formation could be enhanced at higher concentration of indole-3-butyric acid (IBA). Better rooting rate (83.3%) was achieved on a half-strength MS medium with 3.0 /l IBA. Regenerated plantlets were successfully transferred to soil. To investigate the clonal variation in shoot development and shoot elongation by axillary bud culturing, seven plus tree clones were tested, Clonal variation in tissue culturability among plus trees was recognized by the Duncan's multiple range test at the 5% level. Kang Won No. 12 showed the best response on WPM with 1.0/l BAP.

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