• Journal of Life Science
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• v.31 no.3
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• pp.266-279
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• 2021

The present study examined the cytotoxic effects of a Smilax china L. extract (SCLE) in human cancer (A-549, MCF-7, MDA-MB-231, U87-MG, AGS, MKN-74, and SNU-601) and normal MRC-5 fibroblasts, as well as in mesenchymal stem cells derived from dental tissue (DSC). The 50% inhibitory concentration (IC50) values for SCLE were significantly (p<0.05) lower in the cancer cell lines (A-549, MCF-7, MDA-MB-231, U87-MG, AGS, MKN-74 and SNU-601) than in the MRC-5 and DSC cells. Cell growth was significantly (p<0.05) more inhibited in the cancer cell lines treated with 200 ㎍/ml SCLE than in the normal MRC-5 and DSC, and anoikis-like floating cell morphology was observed in the SCLE-treated cancer cells. The cells detached by SCLE treatment were retrieved daily and assayed for viability and telomerase activity. Cells retrieved at 4 days showed significantly decreased viability and telomerase activity (p<0.05), as well as apoptosis-like abnormal morphology, when compared to cells retrieved in the previous 3 days. The ratio of apoptosis and cells in the G1 phase was significantly (p<0.05) increased in the A-549, AGS, and MCF-7 cancer cells treated with SCLE for 4 days compared to untreated controls. However, after SCLE treatment, cell adhesion was not increased by application of an inhibitor of the associated protein kinase (ROCK) that mainly contributes to the increase in cell attachment. This suggests that the cellular detachment by SCLE is probably controlled by a Rho-independent mechanism(s). These observations indicate that SCLE readily induces anoikis in cancer cells and could serve as a potent agent for cancer chemotherapy.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.272-281
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• 2023

It has been known that Paeoniae Radix (PR) contains monoterpene glycosides showing a variety of biological activities such as anti-spasmodic, anti-inflammatory, anti-viral, neuroprotective, and sedative effects. This study aimed to find the cytotoxic compounds isolated from the dichloromethane (CH₂Cl₂)- and ethyl acetate-soluble fractions of PR. As results, thirteen compounds (1-13) were isolated and the chemical structures were identified. In addition, the human alveolar adenocarcinoma cell line (A549) was treated with isolated compounds to determine the cytotoxic effect via evaluation of cell viability. The reduction of A549 cell viability was shown as following order; gallic acid (8) > (2S)-naringenin (9) > methyl gallate (10)>6'-O-benzoylpaeoniflorin (7) > palmitic acid (3). Especially, 7 did not show the cytotoxicity in the human lung fibroblast cell line (MRC-5). The effect of 7 on the cell viabilities in A549 and MRC-5 is firstly reported in this study. Further study is required to find out the cytotoxic mechanism and the selectivity for the cancer cells of 7 in detail.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.15 no.2
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• pp.72-77
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• 2015

Purpose: Deficits of the respiratory chain are reported to be the major cause of Leigh syndrome is said to be the underlying causes. The need for biochemical diagnosis to draw more accurate diagnosis or prognosis to support treatments is rapidly increasing. This study tried to analyze the aspects of clinical characteristics and biochemical diagnosis of mitochondrial respiratory chain complex (MRC) defect in Leigh syndrome, using methods of biochemical enzyme assay. Methods: We included total number of 47 patients who satisfied the clinical criteria of Leigh syndrome and confirmed by biochemical diagnosis. All those patients went through muscle biopsy to perform biochemical enzyme assay to analyze MRC enzyme in order to find the underlying cause of Leigh syndrome. Results: MRC I defect was seen in 23 (48.9%) cases taking the first place and MRC IV defect in 15 (31.9%) following it. There were 9 (19.2%) cases of combined MRC defect. Combined cases of type I and IV were detected in 7 (14.9%) patients while type I and V in 2 (4.3%). The onset age of symptom was less than 1 year old in 28 (59.6%). The most common early symptom, observed in 23 (48.9%), was delayed development, but there were other various neurological symptoms observed as well. In regard with the disease progression, 35 (74.5%) patients showed slowly progressive course, the one that progressed continuously but slowly over 2 years of period. As for Maximum motor development, 22 (46.8%) were bed-ridden state, most of them suffering serious delayed development. Patients showed various symptoms with different organs involved, though neuromuscular involvement was most prominent. Delayed development was seen in all cases. Multifocal lesion in brain MRI study was seen in 36 (76.6 %) cases, taking a greater percentage than 11 (23.4%) cases with single lesion. In MR spectroscopy study, the characteristic lactate peak of mitochondrial disease was identified in 20 (42.6%) patients. Conclusions: Further analysis of clinical and biochemical diagnosis on more extended group of patients with Leigh syndrome will enable us to improve diagnostic precision and to understand the natural course of mitochondrial disease.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.7
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• pp.1202-1207
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• 2006

In this paper we analyze the performance improvement of the M-ary FSK systems for low power and low data rate applications. This contribution presents a unified analysis of its MRC diversity, uncoded and performance in A WGN, m=2, m=3, Rayleigh and one sided Gaussian fading channels using optimum noncoherent demodulation with Reed-Solomon(RS) codes. The results of this paper should be useful as benchmarks of obtainable performance and as a reference for validating the results of simulation studies when slow fading models are applicable.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.5 no.2
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• pp.263-268
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• 2001

This paper analyzes error probability performance adopted the Ray Tracing method efficiently analyzing millimeter wave or optic wave in Inter Vehicle Communication(IVC). Analysis environment analyzed bit error characteristic in 60㎓ band with very powerful to multipath wave, to be large to oxygen absorption and to be good to frequency reuse efficiency. We analyzed bit error characteristic of DS/CDMA system by multi access user in Two-Ray rician channel adopted reflect wave of grand, reflect wave of concrete wall and reflect wave of driving vehicle at side road. Improvement performance is adopted MRC diversity.

• Journal of Electrical Engineering and Technology
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• v.13 no.1
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• pp.250-255
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• 2018

This paper presents a method for minimizing standby power consumption in wireless power transfer (WPT) system via magnetic resonance coupling (MRC) that operates at 6.78 MHz. The proposed circuit controls the required capacitance according to operational condition in order to reduce standby power consumption. Based on an impedance characteristic of the class-E power amplifier, operational principles of the proposed circuit are analyzed. Moreover, to verify the effectiveness of the proposed class-E power amplifier, an 8 W prototype for WPT system is implemented. The measured input power of the proposed class-E power amplifier at standby condition is reduced from 5.81 W to 3.53 W.

• Proceedings of the KIPE Conference
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• 2001.07a
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• pp.260-263
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• 2001

In this paper, we experimentally considered the stability of AT forward MRC with the characteristics of high efficiency and high power density. The converter ratings are input 48V, output 5V/50W, The maximum voltage stress is measured to about 170V of 2 times the input voltage when the input is 58V. The $81.66\%$ of maximum efficiency could be obtained. For the stability of the converter, it is compensated through the error op amp in MC34067. A phase margin and a gain margin for relative stability are measured using HP4194A network analyzer.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.5
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• pp.11-16
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• 2012

Opportunistic transmit cooperative relaying (OTR) system has been interested for its ability to mitigate the fading in wireless channel without multiple antennas in a small terminal. In OTR system, only the relays that the received Signal-to-noise ratio (SNR) from a source is greater than the threshold transmit to the destination. However, the receiving branches of a destination in a realistic system is fixed, the excess number of signals from the transmit relays does not improve the system performance and consequently increases power consumption. In this paper, we adopt Double Opportunistic Transmit (DOT) cooperative diversity system which controls the average number of transmit relays. Although the average number of the transmit relays can be controlled by adjusting the two thresholds in DOT system, the instantaneous number of transmit relays is varying in fading channel. Thus we propose Maximal Ratio Combining (MRC) or Generalized Selection Combining (GSC) according to the number of the signals from relays at the destination. The outage probability of the proposed system is derived in closed form. The analytical results show that the system performance is improved with the number of the branches. Also it is noticed that when the number of the branches is fixed, the outage probability decreases with the increase of the average SNR of S-R path and R-D path.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.386-392
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• 1999

A large-scale culture of hepatitis A virus in human diploid MRC-5 cells was conducted. In a roller bottle culture, the virus was grown to a maximum titer in 3 weeks after infection. Over 95％ of the cell-associated virus was excreted after culturing the infected cells in suspension media without fetal bovine serum for 3 days. The cultured virus was inactivated with formalin, concentrated by ultrafiltration, and partially purified by ultracentrifugation in a non-ionic gradient medium of Renocal. Two separate peak fractions showing high anti-HAY ELISA titer were pooled and about 40％ of HAV antigen was recovered by this purification procedure. Of the partially purified vaccine, the protein pattern in SDS-PAGE and immunogenicity in mice were compared with a commercial HAV vaccine. In SDS-PAGE, the purified vaccine in this study and the commercial vaccine showed almost the same protein pattern. The seroconversion rate of the purified vaccine in mice was not different from that of the commercial vaccine. Therefore, we could prepare a good grade of HAV vaccine by a simple purification procedure although the purification itself was not completed.

• The Proceeding of the Korean Institute of Electromagnetic Engineering and Science
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• v.5 no.1
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• pp.22-30
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• 1994

The error probability of the DS-CDMA / DPSK cellular mobile communication system with CCI canceller and MRC diversity reception technique has been analyzed in the cellular radio channel which is characterized by AWGN, Multi-User Interference(MUI) and m-distribution fading. System capacity i. e., number of user per cell has been derived and the evaluated results are shown in figures as a function of PN code sequence length, fading index, BER, number of diversity branches and $E_b/N_o$, Here, the voice activity factor is assumed to be 3/8, the number of sectors in a cell 3 and Multi-User Iner- ference is modeled as Gaussian process.