• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2002.10a
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• pp.17-20
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• 2002

Hasa TM/TC system is composed of one control center, two reservoirs, six pumping stations and twelve canal systems. For this system we developed water management program which includes flood forecast program, drought reduction program, irrigation scheduling program and database program. With these program we expect that operators can improve the irrigation efficiencies of the irrigation systems due to the timely irrigation on a right place, in a proper quantity and reduce the cost of maintenance and reduce flood and drought damages of the Hasa district. In agricultural engineering respect, the databases including water level, rainfall, the amount of flowing can be useful to the researcher who make a study of hydrology and hydraulics in rural area. Water management program records all of the TM/TC data to MOB format file per 10 minutes.

• Journal of Industrial Technology
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• v.16
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• pp.217-230
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• 1996

This paper deals with storage and retrieval policies for S/R machine with capacity constraints in Man-On-Board AS/RS. It is assumed that storage sequence is based on SFC(spacefilling curve) routine and that storage layout is dedicated by storage policies. We present several heuristic algorithms for storage and retrieval policies which minimize total distance travelled by the S/R machine. These algorithms are based on COI, group COI, frequency of order, similarity between items and capacity of S/R machine. Experimental results of 24 combinastorial policies are provided to illustrate the performance of the heuristics under various rack utilization ratios. In storage policies, the results show that algorithms considering both similarity and frequency are better than those with COI as rack utilization is increasing. And algorithm using group COI is superior to others. In retrieval policies, the method with revision expression is shown to be better than others.

• Biomolecules & Therapeutics
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• v.1 no.1
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• pp.93-97
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• 1993

We have previously reported that an NADPH-dependent succinic semialdehyde reductase was purified homogeneously from bovine brain by several chromatographic procedures, and was found to be a monomeric protein with a molecular mass of 28 kDa (Cho et al., Eur. J. Biochem. 1993). Since succinic semialdehyde is an important intermediate in the ${\gamma}$-aminobutyrate(GABA) shunt and GABA level is associated with various forms of human neurological disorders, we have investigated the effects of anticonvulsant drugs on the succinic semialdehrde reductase. Among the drugs tested, sodium valproate and diphenylhydantoin inhibited the enzyme activity, while some other drugs, barbiturate and chlorpromazine, had no inhibitory effects on the enzyme activity. The purified enzyme was also injected as an immunogen into Balb/c mice to obtain monoclonal antibodies (mob) and several mobs to the protein were produced from the fusion experiments.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.837-842
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• 2013

A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.

• Journal of The Korea Institute of Healthcare Architecture
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• v.6 no.11
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• pp.37-45
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• 2000

The purpose of this study was to analyze the ambulatory care facilities according to the change of medical circumstance and to find the proper ones in Korea. A logical model was used with the data which were obtained from the National Statistical Office, the Ministry of Health and Welfare in Korea and OECD Health Data. A proper ambulatory care facility in Korea is suggested as like as MOB (Medical Office Building). It should be considered for outpatient in distance within 1km long. It's proper size may be designed for 10,000persons to 50,000persons by regions.

• Journal of Korean Institute of Industrial Engineers
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• v.19 no.4
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• pp.97-104
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• 1993

This paper deals with the design problem of a man-on-board(MOB) storage and retrieval warehousing system which is suitable for storing items of small size and light weight. It is assumed that the operator carries out a sequence of retrieving(or storing) operations traveling on a specially designed truck. Considering the operating characteristics of the man-on-board system, an optimal design model is developed in which the investment and maintenance costs of the system are minimized over a time horizon satisfying a set of constructional restrictions. The model is formulated as a nonlinear integer program and a search algorithm is proposed to find an optimum solution.

• Proceedings of the Korea Information Processing Society Conference
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• 2009.04a
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• pp.1120-1123
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• 2009

다양한 기능의 휴대 단말 기기와 무선 환경의 보급으로 무선 인터넷 서비스에 대한 사용자들의 요구가 증가하고 있다. 이와 관련하여 IEEE 802.16 표준은 사용자에게 대용량 데이터 서비스를 제공하기 위한 무선 기술을 정의하고 있다. 또한, IEEE 802.16에 MS의 핸드오버 절차를 추가한 EEE 802.16e 표준 기술은 MS에 대한 핸드오버 절차를 지원하고 있다. 그러나 이러한 핸드오버 절차는 MS이 Serving BS과의 연결을 종료한 이후, 새로운 Target BS에 대한 핸드오버 절차를 수행하므로 사용자는 일시적인 데이터 전송 중단으로 인한 서비스 지연을 경험한다. 본 논문에서는 이러한 문제를 해결하고, 사용자에 대한 빠른 핸드오버 절차를 지원하기 위해 주변 BS들의 위치 정보를 MOB_NBR-ADV를 통하여 MS에게 전송하고, 이를 통해 각 BS들에 대한 MS의 접근 정도를 평가하여 특정한 BS에 대해서만 Association을 수행할 수 있는 기법을 제안한다.

• Korean Journal of Microbiology
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• v.52 no.1
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• pp.110-115
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• 2016

A small plasmid (pDK4) from the Antarctic marine organism Pseudoalteromonas sp. PAMC 21150, was purified, sequenced and analyzed. pDK4 was determined to be 3,480 bp in length with a G+C content of 41.64% and contains three open reading frames encoding a replication initiation protein (RepA), a conjugative mobilization protein (Mob) and a hypothetical protein. PCR-amplified pDK4 was cloned in high-copy pUC19 to yield the fusion vector pDOC153. The chloramphenicol resistance gene was inserted into pDOC153 to give an ampicillin and chloramphenicol-resistant, Pseudoalteromonas - Escherichia coli shuttle vector (7,216 bp; pDOC155). The TonB-dependent receptor (chi22718_IV ) and exochitinase (chi22718_III ) genes from Arctic marine P. issachenkonii PAMC 22718 were cloned into pDOC155 to produce pDOC158 and pDOC165, respectively. Both vector derivatives were transferred into plasmid-free Pseudoalteromonas sp. PAMC 22137 by the triparental mating method. PCR experiments showed that the genes were stably maintained both in Pseudoalteromonas sp. PAMC 22137 and E. coli $DH5{\alpha}$ cells, indicating the potential use of pDOC155 as a new gene transfer system into marine Pseudoalteromonas spp.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.8
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• pp.564-570
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• 2013

Domestic treatment facilities for acid mine drainage (AMD) mostly used a passive treatment process. But some passive treatment facility discharged high manganese concentrations because it is required high pH (>9) for abiotic oxidation of Mn(II) to Mn(IV). This study was focused on the feasibility of biological manganese treatment using the manganese-oxidizing bacteria (Pseudomonas sp. MN5) from AMD and economical application method of it. To investigate the various conditions of water quality the most part of the experiments were based on batch test. And result of it showed that maximum manganese oxidation rate were $10.4mg/L{\cdot}h$ at the pH7. We also performed small column tests in which MOB were attached to the functional polyurethane (FPU) media containing alkaline chemicals. Manganese concentration decreased 42 mg/L to below 6 mg/L. But anaerobic condition formed by excessive bacterial respiration in column resulted in increasing effluent manganese concentration.

• Parasites, Hosts and Diseases
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• v.31 no.3
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• pp.193-200
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• 1993

A total of 230 randomly collected formalin-fixed fecal samples (submitted to the Severance Hospital, Yonsei University) were selected for tests for human cryptosporidiosis. The stool specimens were examined for Cwptosporidium oocysts by acid-fast (AF) stain, auramin-rhodamine (AR) stain, and monoclonal antibody (mAb) OW3 fluorescence method specific for oocyst wall. Of the 230 stool specimens, 21% were identified by the AF method, 22% were identified by the AR method, and 10% were Identified by the mob fluorescence method, indicating that human Crvptosporidium infections have been existing in Korea.