• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.312-320
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• 2000

Abstract The full sequence of the plasmid pKJ36, which was derived from Bifidobacterium longum KJ, was determined and analyzed to construct shuttle vectors between E. coli and Bifidobacterium. The plasmid pKJ36 was composed of 3,625 base pairs with a 65.1% G+C content. The structural organization of pKJ36 was highly similar to that of pKJ50, and the three major ORFs on pKJ36 showed high amino acid sequence homologies with those of pKJ50. The putative proteins coded by these three ORFs were designated as RepB (32.0 kDa, pI=9.25), MembB (29.0 kDa, pI=12.25), and MobB (39.0 kDa, pI=IO.66), respectively. The amino acid sequence of RepB showed a 57% identity and 70% similarity with that of the RepA protein of pKJ50. Upstream of the repB gene, the so-called iteron sequence was directly repeated four-and-ahalf times and a conserved dnaA box was identified. An amino acid sequence comparison between the MobB and MobA of pKJ50 revealed a 48% identity and 61 % similarity. A conserved oriT sequence with an inverted repeat identical to that of pKJ50 was also found upstream of the mobB gene. A hydropathy analysis of MembB revealed four possible transmembrane regions. The expressions of the repB and membB genes were confirmed by RT-PCR. The in vitro translation reaction of pKJ36 showed protein bands with anticipated sizes with respect to each putative gene product. S 1 endonuclease treatment and Southern hybridization suggested that pKJ36 replicates by a rolling circle mechanism via a single-stranded DNA (ssDNA) intermediate. A shuttle vector between E. coli and Bifidobacterium sp. was constructed using the pKJ36, pBR322, and staphylococcal chloramphenicol acetyl transferase (CAT) gene. The successful transformation of the Bifidobacterium strains was shown by Southern hybridization and PCR. The transformation efficiency differed from strain to strain and, depending on the electroporation conditions, with a range between $1.2{\times}10^1-2.6{\times}10^2{\;}cfu/\mu\textrm{g}$ DNA.X> DNA.

• Proceedings of the Korea Multimedia Society Conference
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• 2000.11a
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• pp.542-545
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• 2000

최근 무선 통신 관련 서비스가 활발하게 이루어짐으로써 새로운 종류의 서비스들이 많이 등장하고 있다. 하지만 사업자 측에서 이러한 서비스를 제공하고 사용자로부터 요금을 받을 수 있는 과금 시스템이 아직까지는 미흡한 수준이다. 따라서 본 논문에서는 이러한 상거래 시장 변화에 맞추어 무선 인터넷에서 과금 시스템의 개념을 알아보고, 무선 인터넷 환경에서 안전하고 신뢰할 수 있는 과금 시스템을 제안한다.

• Digital Contents
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• no.10 s.125
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• pp.78-79
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• 2003

지난 1996년 설립돼 최고의 인터넷 커뮤니티 솔루션 업체로 성장한 네트빌이 최근 기업변신을 꾀하고 있다. 유선 인터넷 기반 솔루션 기업에서 유무선 토탈 솔루션 기업으로의 변신이 그것이다. 이 회사는 지난해 말부터 추진해 왔던 모바일 콘텐츠 퍼블리싱 툴 MobPub의 시제품 개발을 최근 완료하면서 이 솔루션을 기업의 새로운 핵심 솔루션으로 내세우겠다는 계획이다. 끊임없는 변화와 도전으로 새로운 시장을 만들어가고 있는 네트빌을 찾았다.

• Journal of Advanced Navigation Technology
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• v.20 no.1
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• pp.93-98
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• 2016

The purpose of this study is to design for MOB antenna attached on safety helmet using microstrip patch antenna. The patch antenna is fabricated in 0.2 mm FR-4 substrate with $64{\times}64mm$ size. The proposed antenna is based on a slot-ring design which cover Inmarsat (1.52 ~ 1.65 GHz) and GPS (1.575 GHz) frequency band. To obtain the optimized parameters, HFSS simulator is used, and antenna is designed by optimized parameters. After antenna was etched, SMA connector was attached to the microstrip feeding line and the result between antenna simulation and measurement was analyzed. Proposed antenna is satisfied the -10 dB bandwidth requirement while simultaneously covering the 1.53 ~ 1.65 GHz.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.1
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• pp.42-47
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• 2005

The possibility of using Moringa oleifera as a ruminant protein supplement was investigated by comparison between nutritive and anti-nutritive value of its different morphological parts with that of conventionally used Leucaena leucocephala leaf meal (LL). Parameters determined were chemical composition, rumen degradable protein (RDP), acid detergent insoluble protein (ADIP), pepsin soluble protein (PESP), non-protein nitrogen (NPN) total soluble protein (TSP) and protein potentially digested in the intestine (PDI). Total phenols (TP) and total extractable tannins (TET) were also evaluated as anti-nutritive factors. In vitro gas production characteristics were measured and organic matter digestibility (OMD) was estimated basing on 24 h-gas production. Crude protein content ranged from 265-308 g/kg DM in M. oleifera leaves (MOL) and seed cake (MOC) respectively. Leucaena leucocephala and Moringa oleifera soft twigs and leaves (MOLSTL) had CP content of 236 and 195 g/kg DM while Moringa oleifera soft twigs alone (MOST) and Moringa oleifera bucks (MOB) had 160, 114 and 69.3 g/kg DM respectively. RDP was highest in (MOC) (181 g/kg DM) followed by (MOL) (177 g/kg DM) and was lowest in MOB (40 g/kg DM). The proportion of the protein that was not available to the animal (ADIP) was (p<0.05) higher in MOL and MOC (72 and 73 g/kg DM) respectively and lowest in LL (29 g/kg DM). The PDI was high in LL (74 g/kg DM) followed by MOC (55 g/kg DM) then MOL (16 g/kg DM). PESP was highest (p<0.05) in MOC followed by MOL then LL (273, 200 and 163 g/kg DM respectively). MOC exhibited highest NPN content (116 g/kg DM) and was lowest in MOB (18 g/kg DM) (p<0.05). Highly (p<0.05) TSP was observed in MOC and MOL (308 and 265 g/kg DM respectively) followed by LL (236 g/kg DM). MOL had negligible TET (20 g/kg DM) when compared with about 70 g/kg DM in LL. Highly (p<0.05) b and a+b values were observed for MOLSTL (602 and 691 g/kg DM respectively) followed by MOL (490 and 538 g/kg DM). Highest c value was observed in MOSTL followed by MOC and MOL (0.064, 0.056 and 0.053 rate/hour) respectively. OMD was highest (p<0.05) for MOSTL followed by MOC and then MOL (579, 579 and 562 g/kg DM respectively). LL exhibited lower (p<0.05) OMD (467 g/kg DM). It was concluded from this study that the high crude protein content in MOL and MOLST could be well utilized by ruminant animals and increase animal performance however, high proportion of unavailable protein to the lower gut of animals and high rumen degradable protein due to negligible tannin content render it a relatively poor protein supplement for ruminants. MOC can be a best alternative protein supplement to leaves and leaves and soft twigs for ruminants.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.21 no.4
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• pp.804-810
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• 2017

Distress accidents occur annually by and failure of sea rescue and time delay lead to fatal accident. In the event of maritime distress, most of the equipments used for search and rescue are in responsible for the position of ships. An MOB equipment is a representative equipment for lifesaving and uses AIS communication method. However, the MOB equipment has problems of interference with existing vessel traffic process and it is difficult to apply because there is no proper certification standard. Therefore, this paper proposes a maritime search and rescue supporting system using smartphone. Utilizing the widely constructed IT infrastructure, it collects position information of the fishing boat and leisure ship using GPS embedded in the smartphone. Also the developed system supports to participate in the rescue by sending the information about the occurrence of the survivor in a certain area to the ship operator.

• 프린팅코리아
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• s.27
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• pp.96-101
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• 2004

하워드 라인골드는 (스마트 몹(Smart Mobs): The Next Social Revolution, 2002))에서 지구촌 사람들이 통신과 컴퓨터 기술의 융합현상(Convergence)에 적응해가는 양상 및 그러한 융합화가 우리 사회에 던지는 의미를 고찰하고 있다. 스마트 몹은, ‘휴대하는 통신과 컴퓨터의 복합기능을 가진 네트워크 기기의 사용을 통해 서로 상대방을 모르는 상태에서도 비슷한 관심사를 가진 사람들을 발견하고, 상호 정보를 교환하고 모이며, 또 네트워크를 형성해서 일치된 협동 행동을 조정할 수 있는 사람들’로 정의된다. 산업화 이후 대량생산과 대량소비의 대상으로 전락했던 ‘군중’ 이 네트워크 기기로 무장해 똑똑하고 능동적인 주체로 거듭난 것이다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2018.11a
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• pp.125-126
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• 2018

최근 해상에서 외부충격으로 정신을 잃고 바다에 빠져 익사하는 사고가 발생하고 있어 해양안전 및 공익을 위해 해양수산부에서는 선박에서 해상으로 추락 시 해로드 앱과 연계되어 조난자의 위치를 자동으로 발신하여 구조요청을 할 수 있는 장치를 개발하였다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2012.10a
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• pp.396-398
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• 2012

선박용 AIS를 비롯한 해양교통시설용 AIS, AIS-SART에 대한 내용을 살펴보고 최근 국제적으로 거론되고 있는 다양한 AIS 관련 응용기술과 국제기구의 논의과정을 살펴본다. 이러한 검토를 통해 국내 AIS 관련 기술의 시장과 국내 관련기관에서의 대응책을 모색하고자 한다.

• The Journal of the Korea Contents Association
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• v.21 no.10
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• pp.476-493
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• 2021

This study is on the simulation conditions of the tentative 'mobilization simulation mode'(MOBSM) and the setting option of major simulation elements. The MOBSM is a training module that practices mobilization of various institutions through a simulation computer similar to actual situations. So far, mobilization exercise(Mob-Ex) is a message simulation method, so it is necessary to convert into a MOBSM because many problems such as fragmentary and practice only by some institutions are raised. Therefore, the theoretical background and previous studies on Mob-Ex and simulation were reviewed to derive the requirements and simulated elements of the MOBSM to meet the purpose of government level exercise and to suggest the critical concepts and the direction of application. The basic requirement is to simulate the main mobilization practices by institution and provide information on the mobilization execution in a nationwide scope. The simulation elements are simulated events and flow charts by mobilization type, simulated range and level by object, simulated contents of material mobilization by institution, key simulated items, DB application, and simulated period, etc. This study will be useful for policy establishment and follow-up research for technology development of MOBSM in the future, and will accelerate the transition to practical mobilization exercise by MOBSM.