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Probabilistic Anatomical Labeling of Brain Structures Using Statistical Probabilistic Anatomical Maps (확률 뇌 지도를 이용한 뇌 영역의 위치 정보 추출)

  • Kim, Jin-Su;Lee, Dong-Soo;Lee, Byung-Il;Lee, Jae-Sung;Shin, Hee-Won;Chung, June-Key;Lee, Myung-Chul
    • The Korean Journal of Nuclear Medicine
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    • v.36 no.6
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    • pp.317-324
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    • 2002
  • Purpose: The use of statistical parametric mapping (SPM) program has increased for the analysis of brain PET and SPECT images. Montreal Neurological Institute (MNI) coordinate is used in SPM program as a standard anatomical framework. While the most researchers look up Talairach atlas to report the localization of the activations detected in SPM program, there is significant disparity between MNI templates and Talairach atlas. That disparity between Talairach and MNI coordinates makes the interpretation of SPM result time consuming, subjective and inaccurate. The purpose of this study was to develop a program to provide objective anatomical information of each x-y-z position in ICBM coordinate. Materials and Methods: Program was designed to provide the anatomical information for the given x-y-z position in MNI coordinate based on the Statistical Probabilistic Anatomical Map (SPAM) images of ICBM. When x-y-z position was given to the program, names of the anatomical structures with non-zero probability and the probabilities that the given position belongs to the structures were tabulated. The program was coded using IDL and JAVA language for 4he easy transplantation to any operating system or platform. Utility of this program was shown by comparing the results of this program to those of SPM program. Preliminary validation study was peformed by applying this program to the analysis of PET brain activation study of human memory in which the anatomical information on the activated areas are previously known. Results: Real time retrieval of probabilistic information with 1 mm spatial resolution was archived using the programs. Validation study showed the relevance of this program: probability that the activated area for memory belonged to hippocampal formation was more than 80%. Conclusion: These programs will be useful for the result interpretation of the image analysis peformed on MNI coordinate, as done in SPM program.

Combined Effects of Filter-feeding Bivalve and Zooplankton on the Growth Inhibition of Cyanobacterium Microcystis aeruginosa (남세균 제어를 위한 동물플랑크톤(Daphnia magna)과 패류(Unio douglasiae)의 단독 및 혼합적용)

  • Kim, Nan-Young;Park, Myung-Hwan;Hwang, Su-Ok;Kim, Baik-Ho;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.48 no.2
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    • pp.108-114
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    • 2015
  • Single - and combined effects of a domestic freshwater bivalve Unio douglasiae (7.6~8.6 cm in shell length) and zooplankton Daphnia magna (1~2 mm in body size) were examined to understand whether they inhibit the growth of harmful cyanobacterial bloom (i.e. Microcystis aeruginosa) in a eutrophic lake. The experiments were triplicated with twelve glass aquaria (40 L in volume); three aquaria without mussel and zooplankton, served as a control, three zooplankton aquaria (Z, density=40 indiv. $L^{-1}$), three mussel aquaria (M, density=0.5 indiv. $L^{-1}$), and three mussel plus zooplankton aquarium (ZM, density=40 indiv.Z $L^{-1}$ plus 0.5 indiv.M/L), respectively. Algal growth inhibition (%) calculated as a difference in the concentration of chlorophyll-a (Chl-a) before and after treatment. Chl-a in all aquaria decreased with the time, while a greatest algal inhibition was seen in the ZM aquaria. After 24 hrs of incubation, Chl-a concentration at the mid-depth (ca. 15 cm) in ZM aquaria reduced by 90.8% of the control, while 63.2% and 79.8% in Z and M aquaria, respectively. Interestingly, during the same period, the surface Chl-a was diminished by 51.9% and 65.4% relative to the control in Z and ZM aquaria, while 27.4% of initial concentration decreased in M aquarium, respectively. These results suggest that 1) this domestic freshwater filter-feeding bivalve plays a significant role in the control of cyanobacterial bloom (M. aeruginosa), and 2) the combination with zooplankton and mussel has a synergistic effect to diminish them, compared to the single treatment of zooplankton and mussel.

Growth Inhibitory Factors of Italian Ryegrass (Lolium multiflorum Lam.) after Broadcasting under Growing Rice from 2014 to 2015 (2014 / 2015년 이탈리안 라이그라스 (Lolium multiflorum Lam.)의 벼 입모 중 파종 재배시 생육저해 요인 분석)

  • Kim, Young-Jin;Choi, Ki-Choon;Lee, Sang-Hak;Jung, Jeong-Sung;Park, Hyung-Soo;Kim, Ki-Yong;Ji, Hee-Chung;Lee, Sang-Hoon;Choi, Gi-Jun;Kim, Won-Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.1
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    • pp.1-6
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    • 2016
  • The growth of Italian ryegrass (IRG) after wintering was very low in 2015 when IRG was broadcasted under growing rice in fall of 2014. To determine growth inhibitory factors of IRG, we examined the growth conditions of IRG in Nonsan region and meteorological conditions in Daejeon nearby Nonsan. Minimum temperature and maximum instantaneous wind speed on Feb. $8^{th}$ and $9^{th}$ of 2015 after wintering of IRG were $8.8^{\circ}C$, 10.7 m/s and $12.4^{\circ}C$, 9.6m/s, respectively. Air temperature was suddenly dropped due to strong wind with snow showers, which had unfavorable effect on root growth of IRG exposed at the soil surface. The minimum temperature and maximum instantaneous wind speed on Feb. $12^{th}$, $13^{th}$, and $14^{th}$ of 2015 were $4.1^{\circ}C$, 11.6 m/s, $-5.6^{\circ}C$, 10.3 m/s, and $-4.7^{\circ}C$, 7.5 m/s, respectively. The growth circumstance of IRG was not good because soil was dried due to drought continued from January. The minimum temperature and maximum instantaneous wind speed on Feb. $26^{th}$, $27^{th}$, and $28^{th}$ of 2015 were $1.8^{\circ}C$, 13.7 m/s, $-3.5^{\circ}C$, 10.6 m/s, and $4.1^{\circ}C$, 6.8 m/s, respectively. The number of wilting of IRG was more than 59% until Mar. $3^{rd}$ of 2015. IRG faced irreparable environment (low minimum temperatures and extreme instantaneous wind speeds) for 9 days from Mar. $4^{th}$ to Mar. $12^{th}$ of 2015. The main reason for the decrease of IRG productivity was collection delay of rice straw after rice harvest because there was continuous rain between Oct. and Nov. of 2014. For this reason, weakly grown IRG under rice straw was withered after wintering. IRG was withered by frost heaving, drought, and instantaneous wind speed in the spring. Furthermore, the root of IRG was damaged while growing in excess moisture in the surface of paddy soil during the winter season due to rain.

Changes in Moisture Contents of Rice-hull Based Root Media and Growth Responses of 'Seolhyang' Strawberry during Vegetative Propagation (육묘 과정 중 포트에 충진된 팽연왕겨 혼합상토의 함수량 변화와 '설향' 딸기의 생장 반응)

  • Park, Gab Soon;Kim, Yeoung Chil;Ann, Seoung Won;Kang, Hee Kyoung;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.33 no.1
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    • pp.47-54
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    • 2015
  • This research was conducted to investigate the changes in moisture retention capacities of expanded rice-hull (ERH)-based root media and their influences on the growth of mother and daughter plants in vegetative propagation of 'Seolhyang' strawberry. The proportion of water at the container capacity of ERH medium was in the range of 20 to 23%. This range was lower than the 60 to 66% of strawberry-specialized medium, the 30 to 34% of soil mother material (SMM) and the 30 to 35% of loamy sand. The moisture content of ERH was reduced to 10 to 12% at 8 hours after irrigation, and there were large variations among replications of ERH medium. Among four kinds of root media formulated to contain ERH, the medium of ERH + coir dust (CD) (55 + 45%, v/v) had 26.5 and 32.5% water contents at 20 and 40 days after irrigation to daughter plants, respectively. The m edia o f ERH + sandy loam (S L) and E RH + S MM showed similar trends i n moisture r etention. The pH and EC i n the all root media tested were in the range of 6.7 to 7.1 and 0.03 to $0.08dS{\cdot}m^{-1}$, respectively. The pHs and ECs measured at 20 and 40 days after irrigation were not significantly different in each root medium. Among the root media formulated to contain ERH, the growth of daughter plants was the highest in the treatment of ERH + SL (55 + 45%, v/v). As the blending rate of coir dust was elevated in the ERH + CD media, moisture retention capacity increased gradually, but the growth of daughter plants became worse even though the medium showed higher moisture retention capacity than other root media tested. The growth of roots and aboveground tissues of daughter plants deteriorated in the root media formulated by blending ERH + perlite (PE) at various ratios. The results of this research suggest the optimum formulations of root media and management of moisture content in raising of strawberry daughter plants when ERH is a component of root media.

The Effects of Ethylene Absorbent on the Quality of 'Fuyu' Persimmon Fruits in MA Package (MA 포장내 에틸렌 흡착 처리가 단감 '부유'의 선도유지에 미치는 영향)

  • Ahn, Gwang-Hwan;Ha, Yeong-Le;Shon, Gil-Man;Song, Won-Doo;Seo, Kwang-Ki;Choi, Seong-Jin
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1278-1284
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    • 2000
  • The study was performed to elucidate the effects of ethylene-absorbent on the quality of 'Fuyu' persimmon fruits in the MA package. Five persimmons were packed in a MA package film (low density polyethylene, 0.055 mm film thickness), and stored at $-0.5^{\circ}C$ for 60 days. Two persimmons were repacked in a MA package with or without ethylene absorbent $(1\;M\;KMnO_4+zeolite)$ and stored at $-0.5^{\circ}C$. Ten days later, these packages was moved to $2^{\circ}C$ or $25^{\circ}C$ storage room to examine the effect of the ethylene-absorbent on the quality of the fruits. Ethylene removal by enclosed ethylene absorbent in MA packaging reduced the rate of fruit respiration at $25^{\circ}C$, so that $O_2$ and $CO_2$ concentration in packing were maintained higher and lower, respectively, compared to control. These effects were not observed, however, in $2^{\circ}C$ post-storage. Fruit firmness and sugar composition were also influenced by ethylene absorbent, showing more delayed flesh softening and higher sucrose concentration in ethylene absorbent treated fruits than control. But ethylene-absorbent treatment lowered glucose and fructose concentration. That shows that ethylene could influence on sugar composition by inhibiting sucrose inversion to glucose and fructose. The production of ethanol and acetaldehyde was reduced by ethylene removal, but the effect was not so high as other quality indices.

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The effect of tumor necrosis factor (TNF)-α to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells (사람의 치수, 치은, 치주인대 세포에 tumor necrosis factor (TNF)-α로 자극 시 matrix metalloproteinase (MMPs)의 분비에 관한 연구)

  • Rhim, Eun-Mi;Park, Sang-Hyuk;Kim, Duck-Su;Kim, Sun-Young;Choi, Kyoung-Kyu;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.36 no.1
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    • pp.26-36
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    • 2011
  • Objectives: In the present study, three kinds of tissues cells (pulp, gingiva, and periodontal ligament) were investigated if those cells express MMP and TIMP when they were stimulated with neuropeptides (substance P, CGRP) or proinflammatory cytokine, TNF-$\alpha$. Materials and Methods: The cells cultured from human dental pulp (PF), gingiva (GF) and periodontal ligament were (PDLF) stimulated with Mock, SP, TNF-$\alpha$, and CGRP for 24 hrs and 48 hrs. for an RNase protection assay and Enzyme Linked Immunosorbent Assay. Cells (PF, GF and PDLF) seeded in 100 mm culture dish were stimulated with SP ($10^{-5}$, $10^{-8}\;M$) or only with medium (Mock stimulation) for 4hrs and for 24 hrs for RNase Protection Assay, and they were stimulated with CGRP ($10^{-5}\;M$) and TNF-$\alpha$(2 ng/mL) for 24 hrs and with various concentraion of TNF-$\alpha$(2, 10, and 100 ng/mL) for Rnase Protection Assay with a human MMP-1 probe set including MMP 1, 2, 8, 7, 8, 9, 12, and TIMP 2, 3. In addition, cells (PF, GF and PDLF) were stimulated with Mock and various concentraion of TNF-$\alpha$(2, 10, and 100 ng/mL) for 24 hrs and with TNF-$\alpha$(10 ng/mL) for 48 hrs, and the supernatents from the cells were collected for Enzyme Linked Immunosorbent Assay (ELISA) for MMP-1 and MMP-13. Results: The expression of MMPs in PF, GF, PDLF after stimulation with SP and CGRP were not changed compared with Mock stimulation for 4 hrs and 24 hrs. The expression of MMP-1, -12, -13 24 hrs after stimulation with TNF-$\alpha$ were upregulated, however the expression of TIMP-3 in PF, GF, PDLF after stimulation with TNF-$\alpha$ were downregulated. TNF-$\alpha$(2 ng/mL, 10 ng/mL, 100 ng/mL) increased MMP-1 and MMP-12 expression in PF dose dependently for 24 hrs. Conclusions: TNF-$\alpha$ in the area of inflammation may play an important role in regulating the remodeling of dentin, cementum, and alveolar bone.

Arthroscopic Rotator Cuff Repair For Partial Articular-Surface Tendon Avulsion (PASTA) Lesion (회전근 개 관절내 부분 파열 환자의 관절경적 봉합술)

  • Lee, Bong-Gun;Cho, Nam-Su;Park, Keun-Ho;Moon, Seong-Cheol;Rhee, Yong-Girl
    • Journal of the Korean Arthroscopy Society
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    • v.13 no.3
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    • pp.242-248
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    • 2009
  • Purpose: To evaluate the clinical and radiologic results of the arthroscopic rotator cuff repair for partial articular surface tendon avulsion lesion. Materials and Methods: Twelve patients with symptomatic, partial articular surface tendon avulsion underwent arthroscopic rotator cuff repair between Mar. 2006 and Sep. 2008. The mean follow-up period was 18.3 months(12~36 months), and the mean age at the time of surgery was 46.9-year-old(19~64 years). Three cases had underwent rotator cuff repair after conversion to full-thickness tear and nine cases had transtendon repair with preserving bursal side cuff. Results: The mean VAS during motion was 6.2 before treatment and 2.0 at final follow-up (p<0.001). The passive forward flexion improved from $163.3^{\circ}$ preoperatively to $169.8^{\circ}$ postoperatively (p=0.038). The mean UCLA score improved from 18.4 preoperatively to 30.1 with 2 excellent, 8 good and 2 fair results at final follow-up. The mean KSS improved from 61.8 preoperatively to 76.8 at final follow-up. By examining the postoperative MR images of 5 patients, complete healing was observed in all of them. Conclusion: Arthroscopic rotator cuff repair may be an effective procedure for partial articular surface tendon avulsion in pain relief and improvement of the range of motion. If the remaining bursal side cuff fibers are intact, transtendon repair procedure with preserving the intact bursal layer of the tendon can be considered. If the remaining bursal side cuff fibers are friable or little, completion from partial-thickness to full-thickness tears with subsequent cuff repair can be considered.

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Anatomical Studies on the Features of Rays in Compression Wood of Korean Red Pine(Pinus densiflora S. et Z.) (소나무(Pinus densiflora S. et Z.) 압축이상재(壓縮異常材)의 방사조직(放射組織) 특성(特性)에 관한 해부학적(解剖學的) 연구(硏究))

  • Chung, Youn Jib;Lee, Phil Woo
    • Journal of Korean Society of Forest Science
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    • v.78 no.2
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    • pp.119-131
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    • 1989
  • This experiment was executed to investigate and compare qualitative and quantitative anatomical features in compression wood, opposite wood, and side wood formed in a bent stem, a straight branch, and an exposed horizontal root of Korean red pine(Pinus densiflora S. et Z.). The respective four discs containing compression wood taken at 20cm interval both in stem and branch as well as a disc containing well developed compression wood from horizontal root were analyzed. Percentage of compression wood and eccentricity showed decreasing tendency with the increasing distance in height direction of stem and length direction of branch. The qualitative anatomical features of compression wood appeared to differ from those of side and opposite wood in very gradual tracheid transition from earlywood to latewood, roundish tracheid shape on cross surface, tracheid distortion at tip on radial surface, existence of intercellular space, and helical cavity in tracheid wall. And the differences in these qualitative features among the compression wood, opposite wood, and side wood became less intensive with the decreasing trends in percentage of compression wood and eccentricity. The quantitative anatomical features in compression wood also appeared to be wider in that respective widths of fusiform and uniseriate ray than those of opposite and side wood, but the heights of fusiform and uniseriate ray in compression wood were smaller than in opposite and side wood. The number of horizontal resin canal(fusiform ray) and uniseriate ray, however, showed no differences among the compression wood, opposite wood, and side wood. And the number of vertical resin canal in unit area, $4{\pi}mm^2$ of compression wood was fewer than that in opposite wood, whereas numerous vertical resin canals contained in a growth ring. These rays of compression wood seemed to be characterized by smaller height and wider width than those of opposite and side wood.

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Comparison of Antioxidant Activities of Enzymatic and Methanolic Extracts from Ecklonia cava Stem and Leave (감태(Ecklonia cava) 줄기 및 잎의 효소적 추출물과 메탄올 추출물에 의한 항산화 활성비교)

  • Lee, Seung-Hong;Kim, Kil-Nam;Cha, Seon-Heui;Ahn, Gin-Nae;Jeon, You-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.9
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    • pp.1139-1145
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    • 2006
  • In this study, antioxidant activities of enzymatic and methanolic extracts from E. cava stem and leave were evaluated by measuring the scavenging activities on 1,1 diphenyl 2 picrylhydrazyl (DPPH), hydroxyl radical, hydrogen peroxide and the inhibitory effects on DNA damage induced by oxidative stress of cells. Enzymatic extracts were prepared by enzymatic hydrolysis of both stem and leave using food grade five different carbohydrases (Viscozyme, Celluclast, AMG, Termamyl, Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase). The enzymatic extracts were lower than methanolic extracts in polyphenol contents, but higher in extraction yield by approximately 30%. The enzymatic extracts were superior to methanolic extracts in DPPH and H2O2 scavenging activities and DNA damage protective effect. There were no significant antioxidant activity difference between stem and leave, but the extracts of leave were relatively better than those of stem. In this study it is suggested that E. cava stem as well as its leave would be a good raw materials for antioxidants compound extraction and enzymatic hydrolysis would be a good strategy to prepare antioxidant extracts from seaweeds.

A Study of Satisfaction About Dental technician's Post and Laboratory in Great Cities (일부(一部) 대도시(大都市) 지역(地域) 치과기공사(齒科機工士)의 직업(職業) 및 직업장(職業場) 만족도(滿足度)에 관한 조사연구(調査硏究))

  • Lee, Tea-Jung;Lee, Myung-Kon
    • Journal of Technologic Dentistry
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    • v.7 no.1
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    • pp.41-51
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    • 1985
  • This study has been to survey the present situation of satisfaction to the occupation and dental laboratory by means of selscting 200 dental technicians who are woked in Pusan, Deagu, Kwangju, Deajun. Questionaires were distributed to them and the summerized results were as follows; 1. In general, the dental technician's satisfaction of the occpation was revealed as moderate(Mn, 2.83), the dental technician's satisfaction of the laboratory was revealas moderate(Mn, 2.80). The highest degree item out of the satisfaction of occpation was the outlook(Mn, 3.23), and the lowest degree item was the advancement and the rise to a high position(Mn, 2.22). The highest degree item out of the satisfaction of laboratory was dental material(Mn, 3.07), and the lowest degree item was the equipment of dust collection(Mn, 2.49) 2. By age, the highest degree item out of the satisfaction of occpation was 30 to 43 group(Mn, 3.28), 50 and over group was the lowest degree item(Mn, 2.89). The highest degree item out of the satisfaction of laboratory was 40 to 50 group(Mn, 3.10), the lowest degree item was 35 to 39 group(Mn, 2.88). 3. By school career, the satisfaction of occpation and dental laboratory were higher in the graduated college group than the graduated highschool group. 4. By personal health state, the highest degree item of the satisfaction of occpation was the health group(Mn, 3.25). The highest degree item of the satisfaction of laboratory was the unhealthy group(Mn, 3.07). 5. By religion, the satisfaction if iccupation of laboratory ws higher in the religious group than the non-religious group. 6. By job career, the highest degree item of occupation satisfaction was 15 to 20 years group(Mn, 3.27), the lowest degree item was 20 and over group(Mn, 3.02). The highest degree item out of the satisfaction of laboratory was 15 to 20 years group(Mn, 3.05), The lowest degree item was 7 to 10 years group (Mn, 2.90). 7. By job position, the highest degree item out of satisfaction of occupation was the chief of laboratory group(Mn, 3.33), the head of laboratory group was the lowest degree item(Mn, 3.07). The hightest degree item out of the satisfaction of laboratory was the head of laboratory group(Mn, 3.10), the lowest degree item was the chief of laboratory group(Mn, 2.88). 8. By the place of employment, the degree of occpational satisfaction by items was found that the highest degree in the group engagel in general hospital(Mm, 3.43), the group engaged in dental clinic and hospital(Mn. 3.24), and the dental laboratory group(Mn. 3.14) in order. The difference among the group was significant emough(P<0.05). The highest degree item out of the satisfaction of laboratory was dental laboratory group(Mn. 2.70). The difference among the group was significant emough(P<0.01). 9. By monthly salary, the highest degree item out of the satisfaction of occupation was ₩500,000 to 600,000 group(Mn. 3.32), the lowest degree item was ₩600,000 and over group(Mn, 3.07). The highest degree item out of the laboratory satisfaction was ₩100,000 to 200,000 group(Mn, 3.03), the lowest degree group was ₩300,000 to 400,000 group(Mn, 2.95). 10. As compared with the degree of satisfaction according to job satisfaction, the highest degree item out of the occupational satisfaction was much satisfied group(Mn, 3.65), the lowest degree item was non-satisfied group(Mn, 2.68). The difference among the group was significant enough(P<0.05). The highest degree item out of the satisfaction of laboratory was non-satisfied group(Mn, 30.9), the lowest degree item was satisfied group(Mn, 2.84). The difference among the group was significant enough(P<0.01).

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