• Title/Summary/Keyword: MKP4

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Inhibition of The Stem Cell Factor-Induced Migration of Mast Cells by Dexamethasone

  • Jeong, Hyun-Ja;Hong, Seung-Heon;Park, Rae-Kil;Kim, Hyung-Min
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2003.11a
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    • pp.76-76
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    • 2003
  • Mast cells accumulation can be causally related with several allergic inflammations. Previous work has demonstrated that glucocorticoids decreased tissue mast cell number and stem cell factor (SCF)-induced migration of mast cells required p38 mitogen-activated protein kinase (MAPK) activation. In the present study, we investigated the effects of dexamethasone on SCF-induced migration of rat peritoneal mast cells (RPMCs). SCF significantly induced migration of RPMCs at 4 h. Dexamethasone dose-dependently inhibited SCF-induced migration of RPMCs (about 90.1% at 100 nM, P<0.05). MAPK p38 inhibitor, SB203580 (20 ${\mu}$M) also inhibited the SCF-induced migration. The ability of SCF to enhance morphological alteration and F -actin formation was also abolished by treatment of dexamethasone. Dexamethasone inhibited SCF-induced p38 MAPK activation to near basal level and induced the MKP-1 expression. In addition, SCF-induced inflammatory cytokine production was significantly inhibited by treatment of dexamethasone or SB203580 (p<0.01). Our results show that dexamethasone potently regulates SCF -induced migration, p38 MAPK activation and inflammatory cytokine production through expression of MKP-l protein in RPMCs. Such modulation may have functional consequences during dexamethasone treatment, especially mast cell-mediated allergic inflammation disorders.

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Comparative study on growth of leafy vegetables grown in a hybrid BFT-aquaponics using Japanese eel, Anguilla japonica and hydroponics

  • Lee, Dong-Hoon;Kim, Jeong-Dae
    • Fisheries and Aquatic Sciences
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    • v.24 no.7
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    • pp.260-275
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    • 2021
  • Aquaponics is a cultivation system that combines aquaculture and agricultural hydroponics. This study investigated the productivity of leafy vegetables cultivated in the hybrid biofloc technology-aquaponics (HBFT-AP) using Japanese eel fed two kinds of diets (Mash, commercial powdered feed and extruded pellet [EP]) and hydroponics (HP).The mash was fed to the fish in a type of a dough mixed with water for 6 weeks (Exp1) and switched to the EP containing 2.7% monobasic potassium phosphate (MKP) for subsequent 6 weeks (Exp2). Leafy vegetables of 8 cultivar were employed in the experiment and water quality [dissolved oxygen (DO, mg/L), pH, water temperature (℃), electrical conductivity (EC; ㎲/cm), turbidity (NTU), TAN (NH3 + NH4+) (mg /L), NO2-N (mg/L), NO3-N (mg/L) and PO4-P (mg/L)] was measured 6 times a week. Leafy vegetable productivity (HBFT-AP vs HP) was compared in respective experiment, which was similar or somewhat higher in HBFT-AP. During the 12-week feeding trial, concentrations of nitrite (NO2-N) and phosphorus (PO4-P) were kept low in variability while total ammonia nitrogen (TAN) and NO3-N levels increased with time in HBFT-AP. At the end of two feeding trials, values of weight gain (WG, %), feed efficiency (FE, %), specific growth rate (SGR, %) and protein efficiency ratio (PER) were higher in Exp2 than in Exp1. As well, higher values in hematocrit (PCV, %), plasma K (mEq/L) and inorganic phosphorus (mg/dL) were found (p < 0.05) in Exp2 where fish were fed the EP (EP: 38.60%, 2.80 mEq/L and 7.04 g/dL; Mash: 33.20%, 1.95 mEq/L and 5.50 g/dL). Leafy vegetables in HBFT-AP using Japanese eel fed the EP with MKP 2.7% had a productivity similar (4 kinds of cultivar) or somewhat higher (4 kinds of cultivar) compared to those in HP. Also, Japanese eel fed the EP showed higher values of Pi (m/dL) and K (mEq/L) in plasma compared to those fed commercial powder diet.

Information on Movement of the Phosphorus(P) Fertilizers in the Turfgrass Soils of Golf Course (골프장의 잔디 토양에서 인산 비료의 이동성 평가를 위한 정보 구축)

  • Chung, Keun-Yook;Baek, Ki-Tae;Ko, Seong-Hwan;Noh, Jae-Goan;Lee, Kyung-Ho;Woo, Sun-Hee
    • The Journal of the Korea Contents Association
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    • v.8 no.8
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    • pp.285-292
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    • 2008
  • This study was initiated to evaluate the Phosphorus(P) leaching potential in the putting green soils and P uptake by the turfgrass in the golf course using the P fertilizers. The turfgrass, Floradwarf bermudagrass(Cynodon dactylon L. PERS,) was planted and grown in the mixture of sand and peat moss in this lysimeter study. Five representative P fertilizers, such as, ammonium polyphosphate (APP), monopotassium phosphate (MKP), MAP(monoammonium phosphate), 0-20-20(liquid), and concentrated superphosphate(CSP, solid) were used in this study. Based on the total P quantity of leachate collected during the whole 12 weeks, MKP and APP are the first group of P fertilizers contributing to the leaching of P, then MAP and 0-20-20 are the second group of P fertilizers causing the P leaching. Finally, CSP is the third group of P fertilizer resulting in the P leaching. However, most of P applied and collected in the lysimeter were leached during the first period of two and four weeks, compared to that of P leached during the second period of six, eight, ten, and twelve weeks. Applications of MAP, APP and CSP, MKP and 0-20-20 in order produced the largest amount of total dry matter. However, APP, MKP and MAP, CSP and 0-20-20 in order showed the largest amount of P uptake. Therefore, based on the data of P leaching, dry matter production, and plant P uptake, it appears that CSP, 0-20-20, and MAP are the environmentally sound fertilizers recommended in the turfgrass putting green soil of golf course.

Comparative Study on Growth and Yield of Far Eastern Catfish Silurus asotus and Leafy Vegetables Grown in Hybrid BFT-Aquaponics, Semi-RAS and Hydroponics (메기(Silurus asotus) 및 엽채류의 성장과 생산을 위한 Hybrid BFT 아쿠아포닉스(HBFT-AP), 반순환 양식(Semi-RAS) 및 수경재배(Hydroponics)의 비교 연구)

  • Lee, Dong-Hoon;Kim, Jin-Young;Lim, Seong-Ryul;Kim, Dal-Young;Kim, Kwang-Bae;Kim, Joo-Min;Kim, Jeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.5
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    • pp.482-495
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    • 2019
  • This study compared the productivity of fish and vegetables grown using the hybrid biofloc technology-aquaponics (HBFT-AP), a semi-recirculating aquaculture system (SRAS), and hydroponics (HP). For the study of fish productivity (HBFT-AP vs. SRAS), fish were provided feed containing 3.0% monobasic potassium phosphate (MKP) for 18 weeks. After the 18-week feeding trial, the average weight of the sampled population (n=100) was not significantly different (P>0.05), while hematocrit (PCV, %), hemoglobin (Hb, g/dL), and plasma K (mEq/L) were significantly different (P<0.05) between the two groups (HBFT-AP: 47.83%, 15.48 g/dL, and 1.39 mEq/L; SRAS 34.83%, 11.81 g/dL, and 2.48 mEq/L). Leaf vegetable productivity (HBFT-AP vs. HP) was compared in three experiments (EXP 1-3), and slower growth was observed in both groups in EXP 2, in which pH was maintained at 5.0 or less throughout the experiment. During the 18-week feeding trial, total ammonia nitrogen (TAN), $NO_3-N$, and $PO_4-P$ levels increased with time in the HBFT-AP system, while the concentration of $NO_2-N$ remained below 0.1 mg/L throughout the study.

Physical and Environmental Properties According to Borax Addition Ratios of Inorganic Filling Adhesive using Magnesia Silicate Phosphate (마그네시아 실리케이트 인산염을 활용한 무기충전 접착재의 붕사 첨가율에 따른 물리·환경적 특성)

  • Kim, Tae-Hyun;Shin, Jin-Hyun;Lee, Sang-So
    • Journal of the Korea Institute of Building Construction
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    • v.17 no.2
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    • pp.141-147
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    • 2017
  • The purpose of this study is to develop an inorganic filling adhesive using MKP and borax based on Dead-burn magnesia and fly ash. First, basic experiments was conducted to derive the proper addition rate of MKP. And this experiment was carried out according to addition ratio of borax. The test items are measured for pot life, flexural strength, compressive strength, adhesive strength, tensile strength, ratio of temperature change, ratio of hardening shrinkage, radon gas and formaldehyde emission. As a result, the proper addition rate of phosphate was 35%. The pot time is about 10minutes, 15minutes and 25minutes according to addition rate of borax. The flexural strength and compressive strength were obtained at 12hours for minimum flexural strength of 8.0MPa and minimum compressive strength of 31.0MPa. The tensile strength was the least 4.1MPa, and the ratio of hardening shrinkage was maximum 2.4% and ratio of heat change was maximum - 0.3%, which satisfied all of the quality standards of 'KS F 4923' (epoxy resin for repairing concrete structures). Both Radon gas and formaldehyde emission was not detected.

Intervention for Reducing Dental Fear and Anxiety of Dental Patients (치과내원 환자의 치과공포 감소를 위한 중재법 적용)

  • Shin, Sun-Jung;Shin, Bo-Mi;Koh, Boo-Il;Bae, Soo-Myoung
    • Journal of dental hygiene science
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    • v.15 no.3
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    • pp.369-376
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    • 2015
  • The purposes of this study were to assess dental fear and anxiety level of patients attending a dental clinic using the dental fear survey (DFS) scale, to apply interventions chosen by patients for reducing dental fear and anxiety and measure their effects. This study surveyed 34 patients who visited a dental clinic in Seoul about their self-rated health and their experience of dental fear, and measured the level of dental fear using DFS. Trained dental hygienists applied interventions desired by the patients for reducing dental fear and anxiety and, for each intervention, examined the patients' satisfaction (very helpful [5 point]~not helpful at all [1 point]). Collected data were analyzed using IBM SPSS Statistics ver. 21.0 through independent t-test or one-way ANOVA for difference in level of fear according to related characteristics, and through Wilcoxon signed rank test for comparison between before and after the intervention. The subjects' mean level of fear (DFS score) was 44.53, which was an average level, and the level of dental fear was relatively high for stimulus-response (2.72). The level of dental fear was higher in those who had experienced pains or indirect dental fear from dental treatment in the past, and those whose subjectively perceived health state was poor (p<0.05). With regard to the applied dental fear intervention, 'Helpful (3.57)' was the most common answer. Overall satisfaction before and after the application of dental fear intervention was generally high as 4.37 and 4.35, respectively, but it decreased slightly after the intervention. In order to lower the level of dental fear, it is considered effective to survey not only the level of dental fear but also patients' need of dental fear interventions and to apply a suitable intervention. It is also required to educate dental workers and to develop related manuals.