• The Korea Journal of Herbology
• /
• v.20 no.2
• /
• pp.115-125
• /
• 2005

Objectives : Polygalae Radix (PR) from Polygalae tenuifolia (Polygalaceae) has been clinically used as a sedative, anti-inflammatory, and anti-bacterial agent. To extend pharmacological effects of PR in the central nervous system (CNS) on the basis of its CNS protective effect, the present study was conducted to identify the effect of PR, whether it shows the neuroprotective action against excitatory neurotoxicity. Methods : To identify the protective effect of PR to excitatory neuro-toxic agent, the present study was focused on the PR effect on cell death, that was caused by applying NMDA to nerve cell, elevation of $(Ca^{2+})_i$, releasement of glutamate, and ROS generation. Result : 1. PR methanol extract, at the concentration range of 0.05 to 5 g/ml, significantly inhibited NMDA (1 mM)-induced neuronal cell death as well as MK-801 (non competitive NMDA antagonist). 2. PR methanol extract $(0.5\;{\mu}g/ml)$ inhibited NMDA (1 mM)-induced elevation of cytosolic calcium concentration $[Ca^{2+}]_i$. NMDA application in the presence of MK-801 $(10\;{\mu}M)$ failed to produce the increase of $[Ca^{2+}]_i$ through all the measurement time. 3. PR methanol extract $(0.5\;{\mu}g/ml)$ inhibited the NMDA-induced elevation of glutamate release. Also, MK-801 showed similar protective effects. 4. PR methanol extract $(0.5\;{\mu}g/ml)$ inhibited the NMDA-induced elevation of ROS generation. Also, MK-801 showed similar protective effects. Conclusion : The present study provides the availability of PR to exert its protective effect on the neuronal cell death in various neurodegenerative pathophysiological conditions.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.34 no.6
• /
• pp.880-884
• /
• 2005

Yellow pigment of Gardenia jasminoides was converted into new pigment by whole-cell biotransformation of thirteen different microbial species. The color value of the biotransformed pigment, which was produced by Streptococcus mutans MK-34, was higher than those of other biotransformed pigments. The biotransformed pigment produced by S. mutans MK-34 dispalyed an characteristic absorption peak at 588 nm and the absorption value increased during the incubation in a jar fermentor. The effects of light and temperature $(60^{\circ}C)$ on storage stability of the biotransformed pigment were investigated. As a result, the biotransformed pigments produced by Streptococcus mutans and Bacillus subtilis were more stable than Gardenia jasminoides yellow pigment during storage.

• Journal of Veterinary Science
• /
• v.22 no.4
• /
• pp.51.1-51.10
• /
• 2021

Background: Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). Objectives: This study aimed to determine the prevalence of OvHV-2 in sheep, goats, cattle, and buffalo in Rawalpindi and Islamabad, Pakistan, by applying molecular and phylogenetic methods. Methods: Blood samples were aspirated from sheep (n = 54), goat (n = 50), cattle (n = 46) and buffalo (n= 50) at a slaughterhouse and several farms. The samples were subjected to heminested polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of the OvHV-2 POL gene and the OvHV-2 ORF75 tegument protein gene. Results: The highest percentage of MCF positive samples was in sheep (13%), whereas goat, cattle, and buffalo had lower positive percentages, 11%, 9%, and 6.5%, respectively. Four OvHV-2-positive PCR products obtained from sheep samples were sequenced. The sequences obtained were submitted to the NCBI GenBank database (MK852173 for the POL gene; MK840962, MK852171, and MK852172 for the ORF75 tegument protein gene). Phylogenetic analysis revealed a close similarity of study sequences with those of worldwide samples. Conclusions: This study is the first cross-sectional study on the prevalence and molecular detection of OvHV-2 in apparently healthy cattle and buffalo that could be carrying OvHV-2 acquired from OvHV-2-positive sheep and goats. The results indicate that OvHV-2 is circulating in Pakistan. Further studies are needed to characterize OvHV-2 and elucidate further its prevalence.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.19 no.4
• /
• pp.345-351
• /
• 2013

The IWRAP program (Inland Waterway Risk Assessment Program) is a useful program for risk assessment of a waterway. However, in the basic version, the function which is used to import AIS data is not included. So users have to prepare the data and input to the program manually. And not all waterways have enough statistical data about passing vessels especially in developing countries as Vietnam. This paper studies the development of a program to pre-process AIS data for using the IWRAP Mk2 program basic version. In addition, it provides users basic information about marine traffic in a waterway such as routes layout, number of passages at a gate classified by type, size and time. The developed program, named TOAIS (Total AIS), was successfully used to pre-process AIS data collected in the Vung Tau waterway-Vietnam. As a result, the IWRAP Mk2 program basic version using data pre-processed from TOAIS could effectively assess the risk of collision in this waterway.

• Honam Mathematical Journal
• /
• v.34 no.4
• /
• pp.519-531
• /
• 2012

Let ${\sigma}_s(N)={\sum}_{d{\mid}N}d^s$. Next, the convolution sums ${\sum}_{k<N/3}{\sigma}_1(3^mk){\sigma}_1(2^n(N-3k))$, ${\sum}_{<N/3}{\sigma}_1(2^mk){\sigma}_1(3^n(N-3k))$, etc., are evaluated for all $N{\in}\mathbb{N}$ with $m$, $n{\in}\mathbb{N}{\cup}\{0\}$.

• Journal of Microbiology and Biotechnology
• /
• v.16 no.7
• /
• pp.1111-1119
• /
• 2006

Beauvericins and enniatins are cyclohexadepsipeptides exhibiting various biological activities on animal systems, including humans. Fusarium oxysporum FB1501 (KFCC 11363P) that produces four different cyclohexadepsipeptides was isolated from soil in Korea and the structures of the four cyclohexadepsipeptides elucidated by HPLC, MS, IR, and NMR analyses. The molecular weights for compounds 1,2,3, and 4 were determined to be 654.5, 784.5, 668.6, and 682.5, respectively, on the basis of ESI-MS measurements. The IR spectra for all the compounds exhibited absorptions for ester $(1,733-1,743\;cm^{-1})$ and amide $(1,649-1,655\;cm^{-1})$ bonds that were very similar to those for beauvericin and enniatins with ester and amide absorptions. The results of the NMR analysis $(^{1}H,\;^{13}C,\;135-DEPT,\;COSY,\;HMQC,\;and\;HMBC;\;in\;COCl_{3})$ revealed that compounds 1,3, and 4 consisted of $_{L}-N-methyl\;valine$ (N-MeVal), $_{D}-{\alpha}-hydroxyisovaleic\;acid$ (Hiv), and 2-hydroxy-3-methylpentanoic acid (Hmp) residues (compound 1: three N-MeVal residues, two Hiv residues, and one Hmp residue; compound 3: three N-MeVal residues, one Hiv, and two Hmp residues; compound 4: three N-MeVal residues and three Hmp residues). Therefore, the compounds were identified as enniatin H (compound 1), enniatin I (compound 3), and enniatin MK1688 (compound 4). Compound 2 was analyzed as beauvericin according to 1D and 2D NMR analyses. This study is the first report related to the co-production of beauvericin with other unusual enniatins, such as enniatin H, enniatin I, and enniatin MK1688, by Fusarium oxysporum.

• The Bulletin of The Korean Astronomical Society
• /
• v.42 no.2
• /
• pp.85.2-85.2
• /
• 2017

The coronal electron density is a fundamental and important physical quantity in solar physics for estimating coronal magnetic fields and analyzing solar radio bursts. To check a validation of coronal electron density distributions (CEDDs) from polarized brightness (pB) measurements with Van de Hulst inversions, we compare CEDDs derived from a polarized brightness (pB) observation [MLSO/MK4 coronameter] and one spectroscopic observation [SOHO/UVCS]. For this, we consider data observed in 2005 with the following conditions: (1) the observation time differences from each other are less than 1 minutes; and (2) O VI doublet (O VI $1031.9{\AA}$ and $1037.6{\AA}$) is well identified. In the pB observation, the CEDDs can be estimated by using Van de Hulst inversion methods. In the spectroscopic observation, we use the ratio of radiative and collisional components of the O VI doublet to estimate the CEDDs. We find that the CEDDs obtained from pB measurements are higher than those based on UVCS observations at the heights between 1.6 and 1.8 Rs (${\times}1.9$ for coronal streamer, 1.2 ~ 1.8 for background corona, and 1.5 for coronal hole), while they are lower than those based on UVCS at the heights between 1.9 and 2.6 Rs (${\times}0.1{\sim}0.6$ for coronal streamer, 0.5 ~ 0.7 for background corona, and 0.6 for coronal hole). The CEDDs of coronal streamers are higher than those of background corona at the between 1.6 and 2.0 Rs: ${\times}1.2{\sim}2.4$ for MK4 and 1.5 ~ 1.9 for UVCS.

• The Bulletin of The Korean Astronomical Society
• /
• v.32 no.1
• /
• pp.33.2-33.2
• /
• 2007

• The Korean Journal of Physiology and Pharmacology
• /
• v.14 no.3
• /
• pp.151-156
• /
• 2010

This study was performed to investigate the role of glutamate neurotransmitter system on gastrointestinal motility in a middle cerebral artery occlusion (MCAO) model of rats. The right middle cerebral artery was occluded by surgical operation, and intestinal transit and geometric center as a parameter of gastrointestinal motility and expression of c-Fos protein in the insular cortex and cingulate cortex were measured at 2 and 12 h after MCAO. Intestinal transit was $66.3{\pm}7.5%$ and $62.3{\pm}5.7%$ 2 and 12 h after sham operation, respectively, and MCAO significantly decreased intestinal transit to $39.0{\pm}3.5%$ and $47.0{\pm}5.1%$ at 2 and 12 h after the occlusion, respectively (p<0.01). The geometric center was $5.6{\pm}0.4$ and $5.2{\pm}0.9$ at 2 and 12 h after sham operation, respectively, and MCAO significantly decreased geometric center to $2.9{\pm}0.8$ and $3.0{\pm}0.3$ at 2 and 12 h after the occlusion, respectively (p<0.01). In control animals, injection of atropine decreased intestinal transit to $35.9{\pm}5.2%$, and injection of glutamate NMDA receptor antagonist, MK-801, decreased intestinal transit to $28.8{\pm}9.5%$. Pretreatment with MK-801, a glutamate NMDA receptor antagonist, in the MCAO group decreased intestinal transit to $11.8{\pm}3.2%$, which was significantly decreased compared to MCAO group (p<0.01). MCAO markedly increased the expression of c-Fos protein in the insular cortex and cingulate cortex ipsilateral to the occlusion 2 h after MCAO, and pretreatment with MK-801 produced marked reduction of c-Fos protein expression compared to MCAO group (p<0.01). These results suggest that modulation of gastrointestinal motility after MCAO might be partially mediated through a glutamate NMDA receptor system.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.43 no.2 s.344
• /
• pp.84-95
• /
• 2006

In H/W implementation for the finite field the use of normal basis has several advantages, especially, the optimal normal basis is the most efficient to H/W implementation in $GF(2^m)$. In this paper, we propose a new, simpler, parallel multiplier over $GF(2^m)$ having a Gaussian normal basis of type k, which performs multiplication over $GF(2^m)$ in the extension field $GF(2^{mk})$ containing a type-I optimal normal basis. For k=2,4,6 the time and area complexity of the proposed multiplier is the same as tha of the best known Reyhani-Masoleh and Hasan multiplier.