• Journal of Food Hygiene and Safety
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• v.11 no.2
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• pp.123-127
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• 1996

The isolation and chemical characterization of yellow food pigments from Monascus purpureus were studied according to the compositions of media. Monacus yellow pigments were isolated and purified by solvent fractionation, silicagel column chromatography, TLC and HPLC. The retention time of Monascus yellow pigments isolated by HPLC was respectively 5 min(I) and 9 min(II) as the yeast malt extract agar(YMA) media and was respectively 4.6 min(III), 5 min(I) 5.7 min(IV), 8.3 min(V), 9 min(II) and 10.7 min(Ⅵ) at the malt extract agar(MEA) media. The structure of monascin(I), ankaflavin(II), 6,11-dihydrorubropunctatin(III), 6,11-dihydromonascorubrin(V) and unknown compounds(IV,Ⅵ) was elucidated by EI-Mass, H and C NMR, UV-visible spectrometer. Therefore, it was suggested that 6,11-dihydrorubropunctatin(III) and 6,11-dihydromonascorubrin(V) are new intermediates of Monascus yellow pigments.

• The Korean Journal of Nuclear Medicine
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• v.14 no.1
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• pp.1-8
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• 1980

Serum TSH levels were measured by radioimmunoassay before and after intravenous administration of synthetic thyrotropin-releasing hormone (TRH) to 15 normal subjects and 55 patients with primary thyroid disease (14 patients with euthyroidism, 24 patients with thyrotoxicosis and 17 patients with hypothyroidism) to evaluate pituitary TSH reserve and its diagnostic availability. The observed results were as follows. 1. In normal subjects, serum TSH responses to synthetic TRH were $3.2{\pm}1.0$ at 0min (baseline TSH level), $8.0{\pm}4.0$ at 10min, $11.7{\pm}5.0$ at 20min, $13.7{\pm}7.1$ at 30min, $9.7{\pm}5.0$ at 60min., $5.2{\pm}2.0$ at 120min. and $3.6{\pm}0.4{\mu}U/ml$ at 180 min. Serum TSH peaked at $20{\sim}30$ minutes and returned nearly to baseline at 180minutes. 2. In euthyroid group, serum TSH responses to synthetic TRH were $3.3{\pm}1.6$ at 0min, $8.6{\pm}8.0$ at 10min, $10.9{\pm}8.5$ at 20min, $12.5{\pm}8.4$ at 30min, $9.0{\pm}5.9$ at 60min, $5.6{\pm}2.6$ at 120min and $3.5{\pm}1.3{\mu}U/ml$ at 180min. No significant difference revealed between euthyroid group and normal subjects (p>0.05). 3. In hyperthyroid group, serum TSH responses to synthetic TRH were $1.5{\pm}0.6$ at 0min, $2.2{\pm}0.8$ at 10min., $2.3{\pm}1.0$ at 20min., $2.4{\pm}1.5$ at 30min., $2.1{\pm}1.1$ at 60min., $1.9{\pm}0.2$ at 120min. and $1.5{\pm}0.8{\mu}U/ml$ at 180min., No response to TRH showed. 4. In hypothyroid group, mean values of serum TSH response to synthetic TRH were 42.0 at 0min., 60.6 at 10min., 124.8 at 20min., 123.0 at 30min., 101.6 at 60min., 64.3 at 120min. and $15.5{\mu}U/ml$ at 180 min., Patients with primary hypothyroidism showed an exaggerated TSH response to synthetic TRH despite their high basal TSH. 5. Side effects attending synthetic TRH administration were transient nausea (59.0%), desire to micturate (59.0%), feeling of flushing(19.7%), dizziness (45.9%), metallic taste (9.8%) and headache (19.7%). Any side effect didn't show in 16.4%. These symptoms began almost immediately after TRH intravenous injection and lasted several minutes, and not related to dose or response in the person experiencing it.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.5
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• pp.543-548
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• 2006

This paper is to be researched into ozone generation character of Bi-Ti-Si type high dielectric ceramic catalyst discharge tube. And conditions of basic experiment are the outside diameter of discharge tube : 52 mm, the length of discharge tube : 350 mm, the frequence : 900 Hz, the temperature of cooling water : $25^{\circ}C$, quantity of flow : 5, 10, 20 L/min, pressure : 1.2, 1.4, 1.6 atm, and distance of discharge gap : 0.4, 0.6, 0.8 mm. Ozone generation characteristics were measured to consumption power. At quantity of flow : 20 L/min, discharge gap : 0.6 mm, pressure : 1.6, and consumption power : 150 W, Maximum ozone generation efficiency of 175 g/kWh was obtained. And a range of maximum ozone generation efficiency was measured below the flow quantity of 20 L/min at below pressure of 1.6 atm. However, Maximum ozone generation efficiency was measured over the flow quantity of 20 L/min at over pressure of 1.6 atm.

• Journal of the Korean Society of Food Culture
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• v.31 no.6
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• pp.643-651
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• 2016

This study was investigated the difference in the quality characteristics of the fermented soybean roasted with different conditions for making chungyukjang. Roasted condition of soybean was decided by pre-test, which was on $140^{\circ}C$ for 21 min, $180^{\circ}C$ 9.5 min, and $220^{\circ}C$ 6 min. Fermented soybean with different roasted condition and conventionally made cheonggukjang were measured for proximate composition, color, pH, amino nitrogen, ammonia nitrogen, free sugar and amino acids. The lightness and yellowness of soybean fermented by conventional method was higher than those of the fermented soybean with roasted soybean. The contents of free sugar of fermented soybean roasted on $220^{\circ}C$ for 6 min (FS220) was the highest among the group and followed by fermented soybean roasted on $180^{\circ}C$ for 9.5 min (FS180), $140^{\circ}C$ 21 min (FS140) and conventional cheonggukjang (FS0). The contents of total free amino acid was highest on fermented soybean roasted on $140^{\circ}C$ for 21 min (FS140) and followed by fermented soybean roasted on $180^{\circ}C$ for 9.5 min (FS180), $220^{\circ}C$ 6 min (FS220) and conventional cheonggukjang (FS0).

• Korean Journal of Veterinary Research
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• v.53 no.2
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• pp.89-93
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• 2013

This study was performed to investigate the effect of Nei Guan (PC-6) moxibustion stimulation on artificial bradycardia of dogs. Xylazine was injected for inducing bradycardia. Rectal temperature, systolic blood pressure, respiratory rate, heart rate were recorded every 10 minutes for 120 minutes. Systolic blood pressure significantly increased on 40 min (p < 0.05) after xylazine injection, compared with those of control group. Heart rate significantly increased on 40 min (p < 0.01), 50 min (p < 0.01), 60 min (p < 0.01), 70 min (p < 0.01), 80 min (p < 0.01), 100 min (p < 0.01), 120min (p < 0.01) after xylazine injection, compared with those of control group. In conclusion, moxibustion of Nei Guan (PC-6) showed recovery effect in xylazine induced bradycardia in dogs.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.4
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• pp.435-441
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• 1998

We examined effects of interleukin $1{\alpha}$ ($IL1{\alpha}$) and phorbol 12, 13 dibutyrate (PDB), an activator of protein kinase C, on mRNA for Prostaglandin H synthase (PGHS) and prostanoid production in cultured ovine meningeal fibroblasts. Immuno- and morphologically-identified fibroblasts were derived from cerebral cortex and white matter from fetal lambs (approximately 120 days gestation) and grown to confluence on glass coverslips in 12 well plates. Levels of prostaglandin $F_{2{\alpha}}$ and the stable hydrolysis product of prostacyclin (i.e., $6-keto-PGF_{1{\alpha}}$) were determined using enzyme immunoassay. Relative amounts of mRNA were determined by in situ hybridization using ovine cDNA for PGHS1. $IL1{\alpha}$ (10 ng/ml) increased mRNA levels over baseline by $62{\pm}19%$ (p＜0.05) at 60 min., $37{\pm}12%$ (NS) at 120 min., and $36{\pm}18%$ (NS) at 240 min (n=12). Levels of $6-keto-PGF_{1{\alpha}}$ were $148{\pm}18%$ pg/ml during baseline, $246{\pm}41%$ pg/ml at 60 min., $248{\pm}40%$ pg/ml at 120 min., and $259{\pm}62%$ pg/ml at 240 min (all p＜0.05) (n=12). $PGF_{2{\alpha}}$ was increased although it wasn't statistically significant. However, $IL1{\alpha}$ decreased $PGE_2$ level significantly (all p＜0.05). PDB $(10^{-6}M)$ increased mRNA levels over baseline by $25{\pm}6%$ after 30 min., $40{\pm}6%$ after 60 min., and $20{\pm}8%$ after 90 min. (n=9) (all p＜0.05). Levels of $6-keto-PGF_{1{\alpha}}$ were $200{\pm}43%$ pg/ml during baseline, $202{\pm}43%$ pg/ml after 30 min. (NS), $268{\pm}58%$ pg/ml after 60 min. (p＜0.05), and $296{\pm}60%$ pg/ml after 90 min. (p＜0.05) (n=9). Levels of $PGF_{2{\alpha}}$ were $178{\pm}26%$ pg/ml during baseline, $300{\pm}30%$ pg/ml after 30 min., $299{\pm}35%$ pg/ml after 60 min., and $355{\pm}32%$ pg/ml after 90 min (all p＜0.05) (n=6). Actinomycin-D (1 mg/ml) prevented increases in mRNA, $6-keto-PGF_{1{\alpha}}$, and $PGF_{2{\alpha}}$ at 60 min. for both $IL1{\alpha}$ and PDB. We conclude that cerebral fibroblasts are avid producers of prostanoids, and that enhanced production of PGHS is responsible for augmented $PGF_{2{\alpha}}$ and prostacyclin production in the presence of an activator of protein kinase C and for decreased $PGE_2$ and increased prostacyclin production in the presence of $IL1{\alpha}$.

• Fibers and Polymers
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• v.7 no.2
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• pp.158-163
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• 2006

Standard extraction procedure for examining chromophoric substances of turmeric was investigated. Acetone and methanol were used as extracting solvents with different extraction procedures and pH levels. GC-MS analysis identified curcumene 2 (6.7 min), feruloylmethane 3 (8.3 min), coumaran 4 (6.09 min), vanillin 5 (6.2 min), and zingiberene 6 (10.5 min) as the major products. Curcumin 1 which has been known as the major chromophoric substance of turmeric was not detected in any samples. The maximum amount of curcumene 2, which was used as the fingerprint product for turmeric dye, was obtained by utilizing presoaking and decanting step with methanol prior to actual extraction step using a waterbath shaker (WMM). The highest relative abundance of curcumene 2 was detected in pH 6 sample followed by pH 5 indicating that the most appropriate pH level was in the range of pH 6-5.

• Exercise Science
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• v.21 no.2
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• pp.231-242
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• 2012

The purpose of this study was to develop the prediction model of VO₂max using submaximal metabolic responses from the Bruce protocol, HR responses at several stages and 1,200 m running record, and to compare and analyse the validity of these prediction models. The subjects were consisted of 255 male(133 male for 1,200 m running). They were participated maximal exercise testing with Bruce protocol, and the metabolic responses were measured in the end of the first(3 minute), second stage(6 minute), and 1,200 m running record. Measurement items were VO₂(㎖/kg/min), VCO₂(㎖/kg/min), VE(L/min), HR(bpm) of 3 and 6 minute, time to HR 150 bpm and 170 bpm, HR difference between Bruce protocol 6 and 3 minute, 1,200 m running record. Analyzing with all variables using enter method, the multiple R of total variable model was 0.642(p<.01), SEE was 4.38 ㎖/kg/min, CV was 10.8 %, but multicolinearity was appeared. The multiple R of 3 minutes model 1 and model 2 were 0.341 and 0.461, SEE was 6.05 and 5.72 ㎖/kg/min, CV was 14.9 and 14.1%, and multicolinearity did not appeared. The multiple R of 6 minutes model 1 and model 2 were 0.350 and 0.456, SEE was 6.03 and 5.74 ㎖/kg/min, CV was 14.9 and 14.2%, and multicolinearity did not appeared. The R of HR 170 and HR 170 model were 0.151 and 0.154, SEE were 6.36~6.37 ㎖/kg/min, CV were 15.7%. The R of 1,200 m running model was 0.444, SEE was 4.82 ㎖/kg/min, CV were 11.9%. In conclusion, with considering usefulness and convenience through the validity of these prediction models, the prediction model of VO₂max recommended 6 and 3 minute model, and the validity of HR model and 1,200 m running model were moderately low.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.5
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• pp.905-910
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• 2004

This study was carried out to observe the change of oriental melon juice quality during manufacture by heating condition and enzyme treatment. To use over-production oriental melon effectively, oriental melon juice was prepared with change of heating temperature and heating time. The heating conditions for minimum filtration time were 98.57$^{\circ}C$ and 11.29 min. The optimum conditions Predicted for separation ratio of clear solution, filtration time and browning color intensity of oriental melon juice were 98$^{\circ}C$, 13 min. In the pectin-degrading enzyme treatment test, turbidity showed minimum value in 4.40 mg％(pectin-degrading enzyme content)， 39.72 mg％ (gelatin content) and 24.09 min (treatment time)．Browning color intensity showed minimum value in 9.33 mg％ (pectin-degrading enzyme content)，44.70 mg％ (gelatin content) and 115.56 min (treatment time). The optimum conditions predicted for turbidity and browning color intensity of oriental melon concentration Juice were 6.6 mg％ (pectin-degrading enzyme content), 33.6 mg％ (gelatin content) and 70 min (treatment time).

• Korean Journal of Environmental Agriculture
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• v.20 no.4
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• pp.225-231
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• 2001

This study was conducted to find out the optimal aeration rates for minimizing odor emission and for increasing biological activities during composting of livestock manure in the enclosed bench-scale reactor system. It was treated with the mixture of poultry manure and sawdust controlled the initial water content of 60%, then aerated continuously at four different aeration rates (0.1, 0.2, 0.4 and 0.6 L/min/kg dry-solids). The average emitted concentration of ammonia in 0.6 L/min/kg dry-solids during composting reached the level of 40% in comparison with that of 0.2 L/min/kg dry-solids. In cases of sulfur compounds such as hydrogen sulfide, methylmercaptan and ethylmercaptan, their concentrations decreased with increasing aeration rates and the emission time was shortened. But they didn't detect in the treatment of 0.6 L/min/kg dry-solids. The biological activity for composting showed a trend of increasing as aeration rates increased. The treatment of 0.6 L/min/kg dry-solids gave the highest biological activity and the best compost quality.