• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6047-6052
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• 2014

Background: Weight loss during chemotherapy has not been exclusively investigated. Macrophage inhibitory cytokine-1 (MIC-1) might play a role in its etiology. Here, we investigated the prognostic value of weight loss before chemotherapy and its relationship with MIC-1 concentration and its occurrence during chemotherapy in patients with advanced esophageal squamous cell carcinoma (ESCC). Materials and Methods: We analyzed 157 inoperable locally advanced or metastatic ESCC patients receiving first-line chemotherapy. Serum MIC-1 concentrations were assessed before chemotherapy. Patients were assigned into two groups according to their weight loss before or during chemotherapy:>5% weight loss group and ${\leq}5%$ weight loss group. Results: Patients with weight loss>5% before chemotherapy had shorter progression-free survival period (5.8 months vs. 8.7 months; p=0.027) and overall survival (10.8 months vs. 20.0 months; p=0.010). Patients with weight loss >5% during chemotherapy tended to have shorter progression-free survival (6.0 months vs. 8.1 months; p=0.062) and overall survival (8.6 months vs. 18.0 months; p=0.022), and if weight loss was reversed during chemotherapy, survival rates improved. Furthermore, serum MIC-1 concentration was closely related to weight loss before chemotherapy (p=0.001) Conclusions: Weight loss both before and during chemotherapy predicted poor outcome in advanced ESCC patients, and MIC-1 might be involved in the development of weight loss in such patients.

• 생명과학회지
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• 제21권3호
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• pp.375-384
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• 2011

허브류의 하나인 로즈마리를 추출, 분획하여 각 용매별 항산화와 항균효과를 관찰하였다. 로즈마리의 항산화효과를 측정하기 위해 DPPH radical 소거능과, ABTS radical 소거능 및 FRAP 활성을 측정하였다. DPPH radical 소거능을 측정한 결과 ethyl acetate분획물의 $RC_{50}$값이 $3.22\;{\mu}g/ml$로 강력한 항산화능을 나타냈으며, water 분획물을 제외한 나머지 분획물(methanol: $5.05\;{\mu}g/ml$, hexane: $6.28\;{\mu}g/ml$, chlorofrom: $5.32\;{\mu}g/ml$, buthanol: $5.74\;{\mu}g/ml$)에서도 강력한 항산화능을 보였다. FRAP활성 또한 ethyl acetate 분획물이 $5.9{\pm}0.3\;{\mu}M/{\mu}g$의 높은 $Fe^{2+}$함량을 나타났으며 water 분획물을 제외한 나머지 분획물에서 높은 $Fe^{2+}$함량을 나타내어 DPPH radical 소거능과 유사한 경향을 보였다. ABTS radical 소거능은 ethyl acetate분획물의 $RC_{50}$값이 $5.05\;{\mu}g/ml$, methanol 추출물의 $RC_{50}$값이 $8.72\;{\mu}g/ml$으로 강력한 항산화능을 나타냈으며 그 외 나머지 분획물들은 DPPH radical 소거능보다 상대적으로 낮은 항산화능을 나타냈다. 로즈마리 단일물질로는 rosmarinic acid, carnosic acid가 DPPH radical 소거능과 FRAP활성에 유사한 경향을 보인 반면 ABTS radical 소거능은 rosmarinic acid만이 $43.16\;{\mu}M$에서 $RC_{50}$값을 나타내었다. 로즈마리의 항균 활성은 Paper disc method를 이용한 Inhibition zone과 MIC (최소 저해 농도)를 측정하여 항균력을 알아본 후 hand plate와 resazurin 환원 실험을 통해 항균제와 식품 보존제로서의 이용 가능성을 알아보았다. 항균 활성 검색 결과 methanol 추출물과 chloroform 분획물이 S. aureus에 대해서 2.5 mg/disc의 농도에서 inhibition zone이 각각 15.3 mm, 15.5 mm로 높게 나타났으며 E. coli에 대해서 14.3 mm, 13.7 mm로 나타나 강력한 항균 효과를 나타내었다. MIC는 S. aureaus 균주와 E. coli 균주 모두 methanol 추출물에서 5 mg/ml의 MIC값을 나타내었고 chloroform 분획물은 2 mg/ml에서 MIC값을 나타내었다. 항균활성의 경우 methanol 추출물과 소수성의 hexane, chloroform 분획물에서 높은 활성을 보였다. 위의 결과를 통해 항균제와 식품 보존제 등의 상품화는 경제적이고 그 활성이 우수한 methanol 추출물의 이용이 가장 이상적인 것으로 판단되어 methanol 추출물을 이용하여 항균제와 식품 보존제로의 이용 가능성을 측정한 결과 항균제로는 hand plate를 통해 5 mg/ml에서 식품 보존제로는 resazurin 환원 실험을 통해 1 mg/ml에서 이용이 가능하리라 생각된다.

• Fisheries and Aquatic Sciences
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• 제5권2호
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• pp.97-102
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• 2002

Pyricularia oryzae (P. oryzae), the cause of rice blast, is one of the most important fungal pathogens of rice. Seventy strains of marine fungi were isolated from marine algae, and it was measured antifungal activity against P. oryzae. Metabolites of marine fungus A-248 which isolated from marine algae showed strong antifungal activity against P. oryzae. The antifungal substance from the metabolites of marine fungus A-248 was extracted with ethylacetate, and then purified by preparative TLC and reverse-phase HPLC. The minimum inhibitory concentration (MIC) value was $0.18\mu g/mL$ for the antifungal activity of the substance purified from A-248 metabolites. The purified substance was similar to antifungal activity of rhizoxin, which is a commercial antifungal agent.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.497-500
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• 1998

A new strain of Bacillus capable of growing upto pH 11 was isolated from a local pond polluted with detergents. This strain elicited unimpaired growth in media supplemented with heavy metals such as As, Cu, Cd, Hg, Ni and Zn. A MIC value of 200, 3, 1.60, 2.25, 7.50 and 3.70 mM was noticed for As, Cu, Cd, Hg, Ni and Zn respectively. Analysis of total DNA revealed the presence of a plasmid of 26 kb. This plasmid was lost by acriflavine treatment to the cultures. Such cured strains were found sensitive to heavy metals. Our findings suggest that incidence of heavy metal resistance is widely distributed and resistant strains could be isolated from heavy metal unpolluted sites.

• Bulletin of the Korean Chemical Society
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• 제31권7호
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• pp.2003-2010
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• 2010

A new class of halobenzyloxy or alkoxy 1,2,4-triazoles and their hydrochlorides were synthesized through cyclization starting from commercially available phenylhydrazine. The structures were characterized by MS, IR and $^1H$ NMR spectra as well as elemental analyses. All the synthesized compounds were screened for their antibacterial activities in vitro against Staphylococcus aureus (ATCC29213), methicillin-resistant Staphylococcus aureus (N315), Bacillus subtilis, Escherichia coli (ATCC25922), Pseudomonas aeruginosa, Shigella dysenteriae, Eberthella typhosa, and antifungal activities against Candida albicans (ATCC76615), Aspergillus fumigatus by broth microdilution assay method. The results of preliminary bioassay indicated that 3-(2,4-difluorobenzyloxy)-1-phenyl-1H-1,2,4-triazole hydrochloride exhibited the best inhibitory activity with an MIC value of 56.25 ${\mu}M$ against P. aeruginosa superior to Chloramphenicol, and showed comparable activity with Chloramphenicol against E. coli (ATCC25922).

• 융합보안논문지
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• 제3권2호
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• pp.57-65
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• 2003

보호프로파일은(Protection Profile)은 특정형태의 제품군이 지녀야 할 보안 목적을 사용자 그룹에서 요구한 명세서이며 보호프로파일의 가정사항은 TOE(Target Of Evaluation)의 물리적, 인적, 네트워크적 관점을 포함하는 사용 환경과 TOE의 사용제한, 잠재적인 자산 가치, 추가적인 적용의 관점을 포함하는 TOE 사용 방법상의 내용을 기술한 것이다. 본 논문에서는 NSA(National Security Agency) 침입탐지 시스템 보호프로파일과 국가기관용 침입탐지 시스템 보호프로파일의 가정사항 항목을 비교 분석하였다.

• 한국염색가공학회지
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• 제11권6호
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• pp.36-42
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• 1999

The antimicrobial activities of the copolymer of N,N'-dimethyl- N,N'-diallyl ammonium chloride(DMDAAC) and diallyl amino(DA) were investigated. The copolymer of DMDAAC and DA was prepared by free radical Polymerization through an intra-intermolecular propagation mechanism ie, cyclopolymerization. The copolymer was, then, reacted with cyanuric chloride for reactivity with hydroxyl group of cellulose. Cotton fabrics were finished by synthesized polymer, and their antimicrobial activities and fastness to launderings were tested. Dichlorotriazinyl DMDAAC-DA copolymer has MIC value of 1ppm against S. aureus and 10ppm against K pneumoniae. The antimicrobial fastness of the finished cotton to launderings were good enough to show colony reduction above 70% against S. aureus and K pneumoniae after 50 repeated laundering in anionic commercial detergent. Optimum treatment concentrations of the polymer were 0.5% in cold pad-batch method, and 0.1% in pad-dry method.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1997년도 추계학술대회
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• pp.19-25
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• 1997

Bicyclomycin(BCM)은 1972년에 일본 Hokkaido, Sapporo지방의 토양균중의 하나인 Streptomyces Sapporoneosis의 배양액으로부터 최초로 분리된 항생물질로서 특히 Gram(-) 박테리아인 Escherichia coli. Klebsiella, Shigella, Salmonella, Citrobacter, Enterobacter cloacae, Neisseria gonorrhoeae등에 대한 선택적인 항균효과를 가지고 있고 Streptomycin, Kanamycin, Chloramphenicol, Tetracyclin, Aminobenzylpenicillin 및 naldixic acid 등에 대해 교차내성을 가지고 있지 않다. BCM은 구조적인 면에서 [4.2.2]bicyclic구조를 가지고있는 특이한 항생물질로서 C(1)-triol부분, diketopiperazine부분 그리고 C(5)-C(5a)exomethylene의 3 부분으로 크게 나눌수 있다(Fig. 1). BGM은 E. coli에 대한 항균력으로서 250-500 $\mu\textrm{g}$/mL의 MIC value를 가지고 있고 급성독성으로서 LD$_{50}$가 4g/kg 이상으로 거의 독성이 없는 것이 특징이다. 현재 Fujisawa pharmaceutical Ltd.로부터 Bicozamycin이라는 상품명으로 설사치료제 용도로 시판되고 있다.

• 산업기술연구
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• 제31권B호
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• pp.113-117
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• 2011

The purpose of this study is to determine the antimicrobial effect of tea tree oil a against pathogens relate to acne. Tea tree oil extracted from Australian Melaleuca alterifolia has been studied extensively for their antimicrobial properties against different type of bacteria and fungi. Tea tree oil has been reported to have antibacterial, antiinflammatory and antitumor activities, among others. By evaluating the antibacterial effect of Tea Tree Oil of Australia, the MIC value of Tea Tree oil against P. acnes was 0.05% and 0.5% against aerobic bacteria. This study showed that Tea Tree Oil has antibacterial effect against bacteria which induce acne. Tea Tree Oil had better antibacterial effect against P. acnes compared to aerobic bacteria. It is promising to develop a nature-based functional material for makeup product, which can cure and prevent acne.

• Food Science and Biotechnology
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• 제14권2호
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• pp.286-289
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• 2005

Active antibacterial compound against periodontitis-causing bacterium Porphyromonas gingivalis was isolated from Kaempferia pandurata and identified as panduratin A. Minimum inhibitory concentration (MIC) value of panduratin A was $4\;{\mu}g/mL$, much lower than those of other natural antibacterial agents. Panduratin A also showed antibacterial activity against Prevotella intermedia ($2\;{\mu}g/mL$), P. loescheii ($4\;{\mu}g/mL$), and cariogenic Streptococcus mutans ($4\;{\mu}g/mL$). Damage on cell wall and perturbation of cytoplasmic membrane of panduratin A-treated P. gingivalis were visualized through transmission electron microscopy. These results suggest panduratin A, exhibiting strong and preferential antiperiodontal and anticariogenic activities, may be utilized in functional foods for prevention of oral diseases.