• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.41-41
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• 2015

BSA-seq technologies, combined Bulked Segregant Analysis (BSA) and Next-Generation Sequencing (NGS), are making it faster and more efficient to establish the association of agronomic traits with molecular markers or candidate genes, which is the requirement for marker-assisted selection in molecular breeding. Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Brassica crops. Even we have breed new clubroot resistant varieties of Chinese cabbage (B. rapa ssp. pekinesis), the underlying genetic mechanism is unclear. In this study, an $F_2$ population of 340 plants were inoculated with P. brassicae from Xinye (Pathotype 2 on the differentials of Williams). Resistance phenotype segregation ratio for the populations fit a 3:1 (R:S) segregation model, consistent with a single dominant gene model. Super-BSA, using re-sequencing the parents, extremely R and S DNA pools with each 50 plants, revealed 3 potential candidate regions on the chromosome A03, with the most significant region falling between 24.30 Mb and 24.75 Mb. A linkage map with 31 markers in this region was constructed with several closely linked markers identified. A Major QTL for clubroot resistance, CRq, which was identified with the peak LOD score at 169.3, explaining 89.9% of the phenotypic variation. And we developed a new co-segregated InDel marker BrQ-2. Joint BSA-seq and traditional QTL analysis delimited CRq to an 250 kb genomic region, where four TIR-NBS-LRR genes (Bra019409, Bra019410, Bra019412 and Bra019413) clustered. The CR gene CRq and closely linked markers will be highly useful for breeding new resistant Chinese cabbage cultivars.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.7
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• pp.986-993
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• 2001

In balance experiments with rats either 25 or 50% of the casein protein in the control diet was replaced with one of the following eight sun-dried tropical biomass products: water spinach plants (WS) (Ipomoea aquatica Forsk), leucaena leaves (LL) (Leuceana leucocephala), duckweed plants (DW) (Lemma minor L.), groundnut foliage (OF) (Arachis hypogaea L.), trichantera leaves (Tric) (Trichantera gigantea), indicago leaves (Ind) (Indigofera hirsuta), mungbean foliage (Mb) (Phaseolus aureus), and cassava leaves (CL) (Manihot esculenta Crantz). The experiment included 102 rats with six individuals per treatment group. In three of the 16 biomass treatment groups, feed intake and weight gain of the rats were unacceptably low, and therefore they were excluded from the statistical evaluation, The crude protein (CP) content of the biomass products varied between 20.9% (Tric) and 33.2% (DW), whereas the content of NDF varied between 18.5% (Ind) and 32.2% (DW) of dry matter (DM). The total content of essential amino acids (g/16 g N) was comparable with that of alfalfa meal, except for GF and Tric, which were inferior. Between plant species, differences in dietary digestibility of organic matter (dOM) and CP (dCP) were observed (p<0.001). Also, the replacement level negatively influenced dOM and dCP (p<0.001). The lowest values for dOM (p<0.001) were observed for diets including biomass products with the highest content of NDF (OF, Tric, Mb, LL). Digestibility of CP was negatively affected by level of protein replacement. Significant (p<0.001) differences were found in N-retention and biological value among diets with different biomass products. The most favourable overall results were obtained for DW, WS and CL. The main factors affecting the nutritive value of the diets tested were their NDF content, dCP and AA profile of the biomass. Also antinutritive component(s) may have influenced the process of digestion and metabolism of some of the biomass products.

• Journal of Soil and Groundwater Environment
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• v.16 no.4
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• pp.38-52
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• 2011

Sorption and sequential desorption experiments were conducted for Zn using a natural soil (NS) in background status by aging (1, 30 and 100 days). The sorption isotherm showed that Zn had high sorption capacity but low sorption affinity in NS. Sequential desorption was biphasic with appreciable amount of sorbed Zn residing in the desorption-resistant fraction after several desorption steps. The biphasic desorption behavior of Zn was characterized by a biphasic desorption model that includes a linear term to represent labile or easily-desorbing fraction and a Langmuirian-type term to represent desorption-resistant fraction. The biphasic desorption model indicated that the size of the maximum capacity of desorption-resistant fraction ($q^{irr}_{max}$) increased with aging in NS. Desorption kinetics and desorption-resistance of Zn in the soils collected from mine tailings (MA, MB and MC collected from surface, subsurface soils and mine waste, respectively) were investigated and compared to the bioavailability to earthworm (Eisenia fetida). Desorption kinetic data of Zn were fitted to several desorption kinetic models. The ratio ($q_{e,d}/q_0$) of remaining Zn at desorption equilibrium ($q_{e,d}$) to initial sorbed concentration ($q_0$) was in the range of 0.53~0.90 in the mine tailings which was higher than that in NS, except MA. The sequential desorption from the mine tailings with 0.01M Na$NO_3$ and 0.01M $CaCl_2$ showed that appreciable amounts of Zn are resistant to desorption due to aging or sequestration. The SM&T (Standard Measurements and Testing Programme of European Union) analysis showed that the sum of oxidizable (Step III) and residual (Step IV) fractions of Zn was linearly related with its desorption-resistance ($q^{irr}_{max}$) determined by the sequential desorption with 0.01M Na$NO_3$ ($R^2$= 0.9998) and 0.01M $CaCl_2$ ($R^2$= 0.8580). The earthworm uptake of Zn and the desorbed amount of Zn ($q_{desorbed}$ = $q_0-q_{e,d}$) in MB soil were also linearly related ($R^2$ = 0.899). Our results implicate that the ecological risk assessment of heavy metals would be possible considering the relation between desorption behaviors and bioavailability to earthworm.

• Journal of Chest Surgery
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• v.37 no.7
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• pp.553-558
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• 2004

Avoiding cardiopulmonary bypass (CPB) in coronary artery bypass grafting (CAB G) has been known to reduce early mortality and morbidity. Diabetes Mellitus is a significant risk factor for adverse early and late outcomes after CABG. We compared the clinical results of off-pump CABG versus on-pump CABG in diabetes patients. Material and Method: 682 patients (424 off-pump CABG and 258 on-pump CABG) underwent isolated coronary artery bypass grafting between January 2001 and June 2003. Data were collected 242 patient who had diabetes. Among them, 154 patients underwent off-pump CABG and 90 patients underwent on-pump CABG. We analyzed the preoperative risk factors and postoperative results between 2 groups. Result: Two groups did not show statistical differences in age, sex, coronary and operative risk factors. Operative time was significantly shorter in off-pump CABG, however, number of grafts was fewer in off-pump CABG. Postoperative inotropic usage was lower in off-pump CABG. Postoperative CK-MB level was lower in off-pump CABG, and ICU stay and ventilation time was significantly shorter in off-pump CABG. However, there was no statistical difference between 2 groups in operative mortality, reoperation rate, perioperative myocardial infarction, wound infection, renal failure, neurological complications and hospital stay. Conclusion: Off-pump CABG group showed less myocardial damage and early recovery. We concluded that off-pump CABG is the more reasonable technique in diabetes patients although two techniques showed good results. The long-term follow up and prospective study may be warranted.

• Journal of Society of Preventive Korean Medicine
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• v.7 no.1
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• pp.79-86
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• 2003

This study was carried out to evaluate cytotoxic effects of Houttuynia cordata $T_{HUNB}$ extracts on A549(lung cancer), B16(mouse melanoma), MDA-MB231(breast cancer) and SNU-C4(colon cancer) cell lines. We have determined by 3-(4, 5-dimethylthiazol-2-Yl)-2, 5-diphenyl-2H-tetrazoliumbromide(MTT) assay. The $150{\mu}g/ml$ concentration of chloroform extract of Houttuynia cordata $T_{HUNB}$ was shown significantly antitoxic activity on MDA-MB231$(65.96{\pm}5.68%)$ and SNU-C4$(55.94{\pm}7.39%)$ cell lines.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.95-95
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• 2002

Human embryonic stem (hES) cells can be induced to differentiate into tyrosine hydroxylase expressing (TH+) cells that may serve as an alternative for cell replacement therapy for Parkinson's disease (PD). To examine in vitro differentiation of hES (MB03, registered in NIH) cells into TH+ cells, hES cells were induced to differentiate according to the 4-/4+ protocol using retinoic acid (RA), ascorbic acid (AA), and/or lithium chloride (LiCl) followed by culture in N2 medium for 14 days, during which time the differentiation occurs. Immunocytochemical stainings of the cells revealed that approximately 21.1% of cells treated with RA plus AA expressed TH protein that is higher than the ratio of TH+ cells seen in any other treatment groups (RA, RA+LiCl or RA+AA+LiCl). In order to see the differentiation pattern in vivo and the ability of in vitro differentiation-induced cells in easing symptomatic motor function of PD animal model, cells (2 $\times$ 10$^{5}$ cells/2${mu}ell$) undergone 4-/4+ protocol using RA plus AA without any further treatment were transplanted into unilateral striatum of MPTP-lesioned PD animal model (C57BL/6). Following the surgery, motor behavior of the animals was examined by measuring the retention time on an accelerating rotar-rod far next 10 weeks. No significant differences in retention time of the animals were noticed until 2 weeks post-graft; however, it increased markedly at 6 weeks and 10 weeks time point after the surgery. Immunohistochemical studies confirmed that a reasonable number of TH+ cells were found at the graft site as well as other remote sites, showing the migrating nature of embryonic stem cells. These results suggest that in viかo differentiated hES cells relieve symptomatic motor behavior of PD animal model and should be considered as a promising alternative for the treatment of PD.

• 한국해양학회지
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• v.22 no.3
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• pp.143-152
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• 1987

The digital data were obtained using Kennedy 9000 magnetic tape deck which was connected to the SMS960 side scan sonar during the field operations. The data of three consecutive survey tracks near Seongsan-po, Cheju were used for the development of this study. The softwares were mainly written in Fortran-77 using VAX 11/780 MINI-COMPUTER (CPU Memory; 4MB). The established mapping system consists of the pretreatment and the digital processing of seafloor image data. The pretreatment was necessary because the raw digital data format of the field magnetic tapes was not compatible to the VAX system. Therefore the raw data were read by the personal computer using the Assembler language and the data format was converted to IBM compatible, and next data were communicated to the VAX system. The digital processing includes geometrical correction for slant range, statistical analysis and cartography of the seafloor image. The sound speed in the water column was assumed 1,500 m/sec for the slant range correction and the moving average method was used for the signal trace smoothing. Histograms and cumulative curves were established for the statistical analysis, that was purposed to classify the backscattering strength from the sea-bottom. The seafloor image was displayed on the color screen of the TEKTRONIX 4113B terminal. According to the brief interpretation of the result image map, rocky and sedimentary bottoms were very well discriminated. Also it was shown that the backscattered acoustic pressurecorrelateswith the grain size and sorting of surface sediments.

• Journal of the Institute of Convergence Signal Processing
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• v.19 no.2
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• pp.47-53
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• 2018

This paper proposes a method to express an MP3 audio file by a series of color QR codes which can be printed on the paper. Moreover, the method can compress the data considerably. Firstly, an MP3 file is divided into many small files which have maximum capacity of binary file of a QR code. Secondly, the multiple files are converted to multiple black-and-white QR codes. Lastly, every three QR codes are combined into color QR codes. When combining, each of three black-and-white QR codes are regarded as red, green, blue components respectively. In this method, the areas of a color QR code where two QR codes are overlapped are expressed by the colors Cyan, Magenta and Yellow. And the areas where three components are overlapped are expressed by white color. Contrarily, the areas that no components are overlapped are expressed by white color. Experimentation result shows that an MP3 file with 8.5MB the original MP3 files are compressed with the compression rate around 15.7. This method has the advantage that can be used in the environments that the internet access is impossible.

• Convergence Security Journal
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• v.5 no.2
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• pp.19-27
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• 2005

As home network is increasing to use, home network industry is developing too. Also, it is to be a popular subject in the network's topics. In this reasons, home network become a important thing because home gateway function is working between access network and home network. In the home network, it relates on the personal computer, home pad, and digital television. But, home gateway is not prepared standard point about techniques. Therefore, many kind of technique want to try for developing of home gateway's functions. Usually, we use ID/PASSWORD method in network control system. But, we found a lot of problems about classical network system while we experienced Jan/25 big trouble. We are considering about that home network system are using same network net. Therefore, seriously we have to check about security and safety at the home network's environment. This report focus on the home network's environment to control for using and efficiency and then it wants to find ways to protect from the internal and external attacks. Existing ID/PASSWORD method it used a electronic signature and the security against the approach from of external watch, the MIB structure of role base and the security of the Home network which leads the authority control which is safe even from the Home network inside it strengthened it used compared to it proposed.

• Journal of Korean Physical Therapy Science
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• v.11 no.3
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• pp.14-27
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• 2004

This study approaches the effects of Balance Training on excercise performance and the prevention from the injuries caused by excercise. The subjects of the Balance Training program are female exercise beginners and the research period covers 8 weeks from January 10 to March 7, 2003. The research objects are 19 female golf beginners in 30s to 40s, who live in Seoul or Seongnam in Gyeonggi province and have played golf less than 6 months. The programs of the Balance Training and exercise performance were conducted to an 11 experimental group among the 19 research objects at the same time, and only the exercise performance program was applied to an 8 control group for 8 weeks. Before and after 8 weeks' application of the research programs to each group, the research subjects were examined, especially the components of their bodies, the balance and the performance capability were measured both before and after the test. The frequency of injuries by exercise was measured after the test, and the difference of the frequency was compared with the frequency before exercise. First, the experimental group, in a measurement of balance, showed that SN, MB, SAr and SAg of static balance decreased in a situation of MEO, MEC, GEO, GEC, TBEO, TBO, FHEO, FEO(p <0.05), but the control group increased. Second, the analysis on the change of exercise performance indicated better improvement in distance, ball speed, and accuracy of the experimental group than the control group(p<0.05). Third, the experience of injuries showed that there were 2 injuries in the experimental group and 11 injuries in the control group. The injured parts were 2 cases in the hands and fingers of the experimental group, and 1 case in the shoulder, 4 in the elbows, 4 in the hands and fingers and 2 in the lumber of the control group. From the above-mentioned results, it is recognized that the Balance Training program improved the exercise performance of female golf beginners and had good effects on the prevention from injuries. Accordingly, if this program is applied to sports-beginners, it will contribute to the improvement of the public health.