• Natural Product Sciences
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• 제25권4호
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• pp.298-303
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• 2019

This study investigated the cytotoxic effects and mechanism of action of asiatic acid in pancreatic cancer cell lines. First, we confirmed the cell viability of MIA PaCa-2 and PANC-1 cells after asiatic acid administration for 48 and 72 h. The viability of MIA PaCa-2 and PANC-1 cells decreased in a dose-dependent manner following asiatic acid administration. To investigate the underlying mechanism, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, annexin V assay, and western blotting. Asiatic acid induced apoptosis and autophagy through activation of AMP-activated protein kinase (AMPK) and inhibition of mammalian target of rapamycin (mTOR) in MIA PaCa-2 cells. Finally, the expression of miR-17 and miR-21, known as oncogenes in pancreatic cancer, was decreased by asiatic acid. These results indicate that asiatic acid has potential as a new therapeutic agent against pancreatic cancer.

• 동의생리병리학회지
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• 제22권2호
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• pp.390-396
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• 2008

Osteoarthritis(OA) diseases are characterized by joint pain, tenderness, limitation of movement, crepitus, occasional effusion, and variable degrees of inflammation without systemic effects. We investigated the effects of Achyrantis radixs cream treatment and low intensity ultrasound in monosodium iodoacetate(MIA) induced experimental osteoarthritis rat. Sprague-Dawley 40 rats of 7-8 weeks, weight $250\;{\pm}\;50$ g were divided into four groups including the control group and ostoarthritis group(30 rats). Histopathological examination, Mankin's score, and immunohistochemical were performed. Histological findings in control group that are similar to those observed in human osteoarthritis, such as disorganization of chondrocytes, erosion and fibrillation of cartilage surface, and subchondral bone exposure. Safranin O-fast green staining revealed that marked diffuse reduction of proteoglycans and chondrocyte treated with MIA. The Mankin's score were closely correlated to the grade of histological findings. The level of Bax and caspase-3 expression decreased experimental groups. This study shows that a Acyranthes Radix cream treatment and low intensity ultrasound exerts a beneficial influence on the severity of chondral lesion in osteoarthritis rats. This treatments could related to a reduced level of chondrocyte apoptosis through anti-apoptotoc capacities of MIA-induced apoptotic protein overexpression.

• 대한본초학회지
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• 제34권4호
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• pp.27-35
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• 2019

Objectives : Osteoarthritis is characterized by degeneration of articular cartilage, which is characterized by chronic pain, stiffness and decrease range of motion. The present study was designed to compare the therapeutic effect of Dioscoreae Rhizoma water extract (DRW) and Dioscoreae Rhizoma 30% ethanol extract (DRE) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : Osteoarthritis was induced by injection of MIA ($50{\mu}{\ell}$ with $80mg/m{\ell}$) into the knee joint cavity of rats. After adaptation period for seven days, rats were divided by 5 groups (n=10/group): normal group, control group, positive control (indomethacin 5 mg/kg), DRW 200 mg/kg treated group, DRE 200 mg/kg treated group (n=10/group). The hind paw weight distribution was measured with the changes of reactive oxygen species (ROS), peroxynitrite ($ONOO^-$) in articulation tissue. Also, the cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factoralpha ($TNF{\alpha}$), interleukin-6 (IL-6), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were investigated by western blot analysis. Results : The administration of DRW and DRE significantly decreased the hind paw weight distribution. The ROS and $ONOO^-$ levels of cartilaginous tissue were significantly decreased in DRW and DRE compared to control group. The results showed that DRE decreased inflammatory cytokines such as iNOS and $TNF{\alpha}$. Also DRE decreased MMP-1 and increased TIMP-1. Conclusions : Based on the above results, Dioscoreae Rhizoma extract seems to have the therapeutic effect on osteoarthritis via suppression of inflammation.

• 대한본초학회지
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• 제35권1호
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• pp.35-44
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• 2020

Objectives : The objective of this study was to investigate the therapeutic effect of Corni Fructus 30% ethanol extract (CFE) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : The subjects were divided into 4 groups ; Normal group (N, n=10), MIA-induced osteoarthritis control group (Con, n=10), indomethacin 5 mg/kg treated group (INDO, n=10), CFE 200 mg/kg treated group (CFE, n=10). Blood and articulation tissues were collected after two weeks of drug administration. Oxidative stress was analyzed with reactive oxygen species (ROS), peroxynitrite (ONOO-). And the Nuclear factor erythroid-2 (Nrf2), heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, glutathione peroxidase-1/2 (GPx-1/2), Nuclear Factor Kappa B p65 (NF-κBp65), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6), Interleukin 1β (IL-1β), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were investigated by western blot. Results : The administration of CFE showed a significant reduction of changes in relative hind paw weight distribution. Reactive oxygen species (ROS) and peroxy nitrite (ONOO-) levels of articulation tissues were significantly decreased in CFE compared to the control group. Western blot measurements of Nrf2, HO-1, SOD, catalase, GPx-1/2 showed that the CFE group was increased compared to the Con group. And western blot measurements of NF-κBp65, COX-2, iNOS, TNFα, IL-6, IL-1β showed that the CFE group was reduced compared to the Con group. Also CFE group decreased MMP-1 and increased TIMP-1. Conclusion : Based on the above results, it can be seen that osteoarthritis is improved when Corni Fructus 30% ethanol extract treated.

• Korean Journal of Acupuncture
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• 제39권4호
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• pp.152-171
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• 2022

Objectives : This study was designed to investigate the effects of Sunbanghwalmyung-eum gamibang on Monosodium iodoacetate-induced osteoarthritis rats. Methods : Forty Sprague-Dawley (SD) rats were divided into 5 groups of 8 rats each. Osteoarthritis (OA) was induced by injecting MIA (2 mg/50 µl) into the joint cavity of the left knee of SD rats belonging to the experimental group, and normal saline was injected into the joint cavity of the left knee instead of MIA in the normal group. To the normal group and the controlled group (OA group), 2 ml of distilled water was orally administered. To the positive control group (Indomethacin group), indomethacin 2 ml at a concentration of 2 mg/kg, to the low concentration group of SHG (Low group), 2 ml of SHG at a concentration of 2 mg/kg, and to the high concentration group of SHG (High group), 2 ml of SHG at a concentration of 4 mg/kg ml was orally administered. The drug was administered for a total of 4 weeks, and histological changes were analyzed by Hematoxylin-Eosin staining and Safranin-O staining. In addition, inflammatory cytokines such as TNF-α, IL-1β, and IL-6, and MMP-13, TIMP-1, and GAGs were immunohistochemically analyzed. Finally, hematological examination, blood biochemical examination, and liver and kidney biopsy were performed. Results : SHG groups (Low and High) inhibited the matrix destruction and damage of the knee joint cartilage in SD rat model, and significantly prevented the reduction in cartilage thickness. In SHG groups, the expressions of TNF-α, IL-1β, IL-6 and MMP-13 were significantly decreased, and the expressions of TIMP-1, GAGs were significantly increased compared with OA group. The safety indicators had no significant differences among five groups. Conclusions : These results show that SHG has cartilage protection capacity, anti-inflammatory effect.

• 한국조경학회지
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• 제43권1호
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• pp.1-15
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• 2015

본 연구는 미국에 있는 베트남전쟁 메모리얼 중 외부공간에 다양한 요소들이 도입되어 공간적 개념을 가지고 만들어진 87개 메모리얼을 대상으로 설계개념, 공간적 특성, 조경디테일의 특성, 기념조각의 특성, 그리고 표현내용을 분석하고, 이에 기초하여 메모리얼에 나타난 기념성을 고찰하였으며, 연구의 주요결과는 다음과 같다. 1. 베트남전쟁 메모리얼 85개소(97.7%)에서는 희생자를 추모하고 국가를 위한 헌신이 강조되었고, 84개소(96.6%)에서 지역 부대 집단의 명예를 고양하는 것이 기본적 설계개념으로 적용되었다. 2. 메모리얼의 공간적 형태는 59개소(67.8%)에서 원형이나 축적 구성을 통하여 독립적인 공간의 완성이 추구되었고, 11개소(12.6%)에서 넓은 부지에 다양한 도입요소나 대지조형을 통하여 공간이 구성되었으며, 기념벽을 주요소로 한 것도 12개소(13.8%)에서 나타났다. 3. 희생자를 추모하기 위해 주로 기념벽이 사용된 곳이 65개소(74.7%)였고, 대부분의 메모리얼에서 다른 전쟁메모리얼처럼 국가 및 지역, 부대의 명예 고양을 위해 기와 엠블렘이 사용되었다. 이 밖에 빈 의자, 기념비나 마커, 기둥 및 열주, 폰드 및 수로, 헬리콥터 등이 일부 메모리얼에 도입되었다. 4. 메모리얼에서는 V자형 검은색 기념벽을 자주 사용하였기 때문에, 기념조각은 상대적으로 적은 21개소(24.1%)에서 도입되었다. 이 중에서 승리를 고무하는 영웅적인 전투 장면보다는 병사상이나 부상 장면을 묘사한 사실적 조각이 16개소(18.4%)로 대부분이었고, 일부에서 추상조각 및 은유조각이 사용되었다. 5. 전사자 및 실종자 등 희생자를 추모하기 위해 기념벽에 전사자 및 실종자 이름을 새기거나, 일부에서는 전사자 및 실종자의 사진 및 추모시를 음각하였고, 'DUTY, HONOR, COUNTRY'와 같은 상징적 문구를 적었으며, 전쟁으로 인하여 사회적으로 큰 이슈가 되었던 전쟁포로 및 실종자에 대한 관심을 나타내는 MIA/POW 이미지를 활용하였다. 6. 미국에서는 기념벽을 중요한 요소로 활용하여 전사자 실종자 참전군인의 이름을 새기고, 성조기 부대기 주기 등을 게양하며, 'ALL GAVE SOME, SOME GAVE ALL' 및 'DUTY, HONOR, COUNTRY'를 자주 표현하여 희생자를 추모하고, 명예를 고양하는 민주주의적 애국주의를 강조하였다. 반면, 베트남에서는 전쟁승리 및 민족 저항정신을 강조하였고, 사회주의의 대표적 양식인 기념조각 및 기념탑을 자주 사용하였으며, '조국은 당신을 기억한다'는 의미의 'TO-QUOC-GHI-CONG(토-국-기-공(土-國-記-功))'을 새기어, 민족주의, 사회주의, 애국주의를 강조하였다는 점에서 대조적이다. 향후 베트남 및 미국 이외에 베트남전쟁 참전국인 한국 및 오스트레일리아에 있는 베트남전쟁 메모리얼에 대한 분석을 통하여 국가적 기념성에 대한 비교 연구가 필요하다.

• 대한치과교정학회:학술대회논문집
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• 대한치과교정학회 2004년도 제37회 학술대회
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• pp.81.3-82
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• 2004

• 대한치과교정학회:학술대회논문집
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• 대한치과교정학회 2001년도 제34회 학술대회
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• pp.77.2-77.2
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• 2001

• 대한치과교정학회:학술대회논문집
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• 대한치과교정학회 2002년도 제35회 학술대회
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• pp.29.2-29.2
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• 2002

• 대한치과교정학회:학술대회논문집
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• 대한치과교정학회 2005년도 제38회 학술대회
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• pp.65.2-65.2
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• 2005