• Bulletin of the Korean Chemical Society
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• v.30 no.9
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• pp.2021-2026
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• 2009

Toluene, xylene and styrene are volatile organic solvents that are commonly used in mixtures in many industries. Because these solvents are metabolized and then excreted in urine, their urinary metabolites are thought to be biomarkers of occupational exposure to these solvents. Therefore, a simple, rapid, and yet reliable analytical method for determining the metabolites is required for accurate biological monitoring. In the present study, a simple and rapid HPLC-UV method was developed for the simultaneous determination of eight major metabolites of toluene, xylene and styrene: hippuric acid (HA), mandelic acid (MA), o-, m- and p-methylhippuric acids (o-, m- and p-MHAs), and o-, m- and p-cresols. A monolithic column was employed as the stationary phase and several conditions, including flow rate, composition of mobile phase and column temperature, were variables for the optimization of the chromatographic resolution. All eight metabolites were successfully resolved within 5 minutes in 10% aqueous ethanol containing 0.3% acetic acid and 1.6% $\beta$-cyclodextrin, using a flow rate gradient of 1.0 - 5.0 mL/min at 25 ${^{\circ}C}$. The performance of this method was validated by linearity, intra- and inter-day accuracy, and precision. The linearity was observed with correlation coefficients of 0.9998 for HA, 0.9999 for MA, 0.9989 for o-MHA, 0.9998 for m-MHA, 0.9991 for p-MHA, 0.9997 for o-cresol, 0.9998 for m-cresol, and 0.9986 for p-cresol. The intra- and inter-day precision of the method were less than 5.89% (CV) and the accuracy ranged from 92.95 to 106.62%. The validity was further confirmed by analysis of reference samples that were prepared by the inter-laboratory quality assurance program of the Korea Occupational Safety and Health Agency (KOSHA, Seoul, Korea). All measured concentrations of the analytes agreed with the certified values.

• 한국기계연구소 소보
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• s.16
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• pp.75-81
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• 1986

본 논문에서는 기계부품 요소의 설계와 분석에 사용되는 대화식 Graphics package KIMMPAK 을 설계.구현하였다. 2차원의 Plotting 루틴과 Device driver로 구성된 KIMMPAK은 TEKTRONIX 그래픽 터미널을 Drive할 수 있는 PLOT10-TCS와, IBM-PC용의 Multi-HALO(MHA¬LO), 그리고 HP Plotter의 PLOT-21 그래픽 라이브러리를 이용하여 Device Driver를 구축하였다. 이로 인한 KIMMPAK의 Device 독립성의 증가는 패키지 자체의 호환성 및 신뢰성을 높이고 있다. KIMMPAK은 VAX/VMS V4 . 2 운영체제하에서 FORTRAN-77의 부프로그램 형태로 구현되어 있으며, IBM-PC에서 사용 가능하도록 MS-FORTRAN으로 변화된 PC용 KIMMPAK이 따로 설계.구현되었다.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.2
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• pp.223-229
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• 1996

This experiment was conducted to investigate the protein sparing effect of rumen protected lysine(RPLys) and methionine hydroxyl analogue(MHA) in sheep. The treatments were $T_1$ (CP 15% + RPLys 0%), $T_2$ (CP 12% + RPLys 0%), $T_3$ (CP 12% + RPLys 0.4%) and $T_4$ (CP 12% + RPLys 0.4% + MHA 0.3%). Ruminal characteristics, in situ and in vitro digestibility and nitrogen retention rate were measured in sheep receiving different combinations of dietary supplement. The results are summarized as follows: 1. Ruminal pH and VFA concentrations were not affected by the treatments. Ruminal ammonia-N concentration was high in sheep fed diets $T_2$, $T_3$ and $T_4$ with the highest value in the $T_4$ treatment(p<0.05). 2. The digestibilities of dry matter and organic matter were not affected by the treatments. 3. Nitrogen losses through feces and urine were the highest with $T_1$ (p<0.05) and nitrogen retention rates of groups $T_1$, $T_2$, $T_3$ and $T_4$ were 18.6, 32.4, 35.5 and 27.5% of nitrogen intake, respectively, indicating that RPLys supplementation improved nitrogen retention in sheep.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.2-5
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• 1996

Orissa is one of the major rice growing states of India. The state covers an area of 15.54 mha with 6.00mha cultivable holding is 1.46 ha. The power input is much below the national average of 0.87 hp/ha. Recently, the Government of Orissa has accorded equal status to Agriculture as given to the Industry with determination to increase the power input for agricultural production to2.54 hp/ha so as to obtain an average rice productivity of 3.0t/ha. Appropriate power units so as to be available with in the purchasing power of the small size farm owners in lieu of a pair of bullocks appear to be the right approach and is found to be in line with the highly successful Japanese and Korean model of small farm mechanization . The state needs 0.25m units of small tillers and tractors per year. The present population of tractors and power tillers in the state is about 11000nos. In India, two manufactures in collaboration with Japanese firms started manufacturing of power tiller sin 197 . The present production of power tillers in the country is about 1000 unit per year which is inadequate to meet the demands of all the stats. To meet the demands of the Orissa state, three Chinese model power tillers imported by local agencies are being supplied to the farmers during the past two years. Encouraged by the demand of power tillers in the state, three local manufacturing units located at Bhubanewar have come up with their own model of light weight power tillers(1.5 to 3.0 hp) during the past one year. These machine have been evaluated in the farms of the university and have been found to be promising . There is a target of supplying during 1996-97. The local manufacturers are in need of international collaboration for technical knowhow to manufacture these machine economically to meet the requirement of the farmers.

• The Korean Society of Law and Medicine
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• v.19 no.2
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• pp.99-137
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• 2018

Under the Korean law, there are two routes of involuntary civil commitment of the mentally-ill: involuntary commitment process under the Mental Health Act (MHA) and the guardian's commitment backed up by family court approval under article 947-2 (2) of the Civil Code. Despite of the recent fundamental revision of MHA in 2016, the Korean involuntary commitment law has still serious flaws, especially the lack of due process like prior notice, hearing, and independent guardian ad litem for the mentally-ill, which has been pointed out also by the Korean Constitutional Court. Thus, a re-revision is inevitable, and this time, we should proceed to rebuild the underlying structure of involuntary commitment. In this regard, it is crucial to eliminate the old-fashioned and unjustifiable burden as well as power of the so-called responsible person to protect the mentally-ill and to readjust the causes and standings to petition of the various types of involuntary commitment process. Also it is necessary to repeal involuntary commitment by guardian under the Civil Code, article 947-2 (2), which can no longer harmonize with modern involuntary commitment system.

• Microbiology and Biotechnology Letters
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• v.50 no.3
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• pp.328-336
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• 2022

Aptamers are short, chemically synthesized, single-stranded DNA or RNA oligonucleotides that fold into unique three-dimensional structures. In this study, we aim to determine the antibiofilm activity and binding specificity of the six polyclonal DNA aptamers (S15K3, S15K4, S15K6, S15K13, S15K15, and S15K20) on Staphylococcus aureus BPA-12 and Escherichia coli EPEC 4. Aptamer S15K6 showed the highest percentage of antibiofilm activity against S. aureus BPA-12 (37.4%) as shown by the lowest OD570 value of 0.313. Aptamer S15K20 showed the highest percentage of antibiofilm activity against E. coli EPEC 4 (15.4%) as shown by the lowest OD570 value of 0.515. Aptamers S15K13 and S15K20 showed antibiofilm activities against both S. aureus BPA-12 and E. coli EPEC4, and thus potentially have broad reactivity. Furthermore, based on the binding capacity and Kd values from our previous study, the binding specificity assay of selected polyclonal DNA aptamers (S15K3 and S15K15) against S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, S. agalactiae, E. coli MHA-6, and Listeria monocytogenes were performed using qPCR. Aptamers S15K3 and S15K15 showed specific binding to S. aureus BPA-12, E. coli EPEC 4, S. aureus BPA-6, and S. agalactiae, but could not bind to E. coli MHA-6 and L. monocytogenes. Therefore, this study showed that the polyclonal DNA aptamers have antibiofilm activity and were able to bind to S. aureus BPA-12 and E. coli EPEC 4 bacteria.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.233-233
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• 1996

배지의 영향에 대하여 검토하기 위하여 SDA, MHA, NA 및 BHIA를 사용하였으며, 시험균주 중 Pseudomonas aeruginosa에 대하여만 NA에서의 MIC 값이 낮게 나타났을 뿐 나머지 균주에 대하여는 배지에 따른 항균력 변화가 관찰되지 않았으며, 배지의 pH를 5.4-8.4까지 변화시켰을 때 pH 변화에 따른 DA-1131의 유의적인 항균력 변화와 접종균량에 따른 항균력 변화가 관찰되지 않았다. Methicillin-resistant Staphylococcus aureus(MRSA)에 대하여는 DA-1131과 vancomycin 간의 상승 및 상가작용이 관찰되었고, P. aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae 및 Serratia marcescens 등의 균종에 대하여는 ciprofloxacin의 상가작용이 확인되었다. Sisomicin은 P. aeruginosa에 대한 상승 및 상가작용이 인정되었으나 ceftazidime은 DA-1131에 의한 길항작용이 관찰되었다.

• Journal of KIBIM
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• v.3 no.2
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• pp.10-18
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• 2013

In the architectural design industry recent BIM system was introduced to the vast amount of information generated by the process, who need it and require the need to manage the construction of information related to production, storage, delivery. In addition, for the project to perform the work involved collaboration between organizations is essential. The purpose of this study was to BIM standard for the system to effectively utilize BIM technology and development of the BIM business support collaboration system, to establish a BIM application environment, a variety of information in the field of architectural design that can be used practically efficient transcription.

• Proceedings of the KIEE Conference
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• 2003.07b
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• pp.1000-1002
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• 2003

The characteristics of eddy current sensor has been analyzed by using numerical analysis which is performed by modeling eddy current sensor and analytic object into three-dimension axis-symmetry in this paper. The eddy current sensor is modeled as cylindrical shape with variation of inside and outside diameter and frequency range between 1[kHz] and 1[MHa] for numerical analysis. The value of impendence on eddy current sensor depending on frequency variation was calculated through numerical analysis. The characteristics of eddy current sensor can be studied by normalized impendence which is gained from the calculated impendence. Therefore, sensitivity of eddy current sensor depending on frequency, inside and outside diameter can be known by investigating diameter of half circle impendence and its locus.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2006.05a
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• pp.997-1000
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• 2006

In this paper, we have used the 900Mha RF band and produced the transmitter-receivers to measure the distance of moving range of a crane. We selected the analog signal of 450Khz middle frequency band of the RF Baseset module, and the property data of phase response is analysed to estimate the range distance. We have earned the good results from the experiment.