• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.387.1-387.1
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• 2002

Aging and estrogen cleficiency after menopause induce bone loss and result in osteoporosis. This study was investigated effects of n-hexane fraction (Hx) extracted from Astragali Radix on osteoporosis with osteoblast-like cell line (MG-63 and Saos-2) and an ovariectomized (OVX) rat model. Proliferation of osteoblast-like cells. MG-63 and Saos-2. was tested with MTT and alkaline phosphatase (ALP) assays. (omitted)

• Preventive Nutrition and Food Science
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• 제2권2호
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• pp.167-173
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• 1997

The inhibitory effects of kale juice on the growh and DNA incorporation of human cancer cells, using HT-29 colon cancer cells, MG-63 osteosarcoma cells, AGS gastric adenocarcinoma cells and K-562 leukemia cells, were studied. The growth of human cancer cells were inhibited in the presence of kale juice (10, 20 nd 40$\mu$l/ml) and the effects were the juice concentration- and incubation time-dependent up to 6 days. When 20$\mu$l/ml of kale juice was added to the media of HT-29, MG-63, AGS and K-562 cancer cells, the cell growth after 6 or 4 days of incubation was retarded by 83~95% of control group. Morphological changes of HT-29 colon cancer cells wre studied under inverted microscope. As the concentration of kale juice increased up to 20$\mu$l/ml, degree of cell aggregation was decreased. Moreover, the DNA incorporation o AGS gastric adenocarcinoma cells and MG-63 osteosarcoma cells which were labeled with [$^3$H] thymidine was significantly reduced after 2 days of incubation at 37$^{\circ}C$ with kale juice. Therefore, we concluded that kale juice strongly decreased the growth of various human cancer cells.

• 한국재료학회지
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• 제16권10호
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• pp.606-613
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• 2006

This study was purposed to evaluate the influence of anodically oxidized film on titanium (Ti) onto MG-63 osteoblast-like cell attachment and activity. Only scratch lines created by polishing were seen in ASR and ANO-1 groups. About $1.5{\mu}m$-thick homogeneous oxide film which has pores of about $0.5{\mu}m$ diameter were formed in ANO-12. The crystalline structure of the oxide films formed by anodization in phosphoric acid electrolyte was $TiP_2O_7$. The total protein amounts of ANO-1 and ANO-12 groups showed higher values of maximum protein amount than that of AS-R group. At 3 days of incubation, total protein amount showed higher value in ANO-2 when comparing to that of AS-R (p<0.05). Based on the results of ALPase activity test, the degree of MG-63 cell differentiation for initial mineralization matrix formation was similar. For all the test groups after 1 day of incubation, MG-63 cells grew healthily in mono-layer with dendritic extensions. After incubation for 3 days, the specimen surfaces were covered more densely by cells, and numerous micro filaments were extruding to the extracellular matrix.

• Molecular & Cellular Toxicology
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• 제2권2호
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• pp.97-105
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• 2006

Several features of the implant surface, such as roughness, topography, and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of ceramic-coatings on Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on Zr (Zrconium-coated surface), Nb (Niobium-coated surface), and control (Uncoated Titanium) Ti. The morphology of these cells was assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1,152 elements). The appearances of the surfaces observed by SEM were different on each of the three dental substrate types. MG63 cells cultured on Zr, Nb and control exhibited cell-matrix interactions. In the expression of several genes were up-, and down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.

• Molecular & Cellular Toxicology
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• 제3권1호
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• pp.36-45
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• 2007

Several features of the implant surface, such as topography, roughness, and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of different-coatings on Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on HA (Hydroxyapatite coating on Titanium), Ano (HA coating on anodized surface Titanium), Zr (zirconium-coating on Titanium), and control (non-coating on Titanium). The morphology of these cells was assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1,152 elements). The appearances of the surfaces observed by SEM were different on each of the three dental substrate types. MG63 cells cultured on HA, Ano, Zr, and control exhibited cell-matrix interactions. In the expression of several genes were up-, and down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2001년도 춘계연합학술대회 초록
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• pp.302.1-302
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• 2001

• 융합정보논문지
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• 제11권7호
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• pp.264-271
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• 2021

본 연구에서는 쌍발이모자반 추출물의 항산화능 및 항염능을 확인하였다. 항산화능 실험에는 폴리페놀 농도측정, 플라보노이드 농도 측정, DPPH 실험, ABTS 실험, NO 실험, FRAP 실험을 실시하였다. 폴리페놀의 경우 18.99±0.69 mg/g으로 나타났다. 플라보노이드의 경우 11.89±1.16 mg/g으로 나타났다. DPPH 실험에서는 19.78 mg ascorbic acid / g extract의 항산화능을 나타내었으며, ABTS 실험에서는 63.64 mg ascorbic acid / g extract의 항산화능을 나타내었으며, NO 실험에서는 7.966 mg ascorbic acid / g extract의 항산화능을 나타내었다. FRAP에서는 쌍발이모자반 추출물의 1 mg이 ascorbic acid 2.089 ㎍의 환원력을 보였다. 한편 세포실험에서는 세포 독성과 LPS로 유도된 염증에 대한 항염능을 알아보았다. 세포독성의 경우 모든 농도에서 80%이상의 세포 생존률을 보였으며, NO 생성 억제능의 경우 100 ㎍/mL 농도에서 30.64±0.23%의 염증 억제능을 보여 쌍발이모자반 추출물이 항염능을 가진 화장품 원료로서 사용가능함을 보였다.

• 약학회지
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• 제53권4호
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• pp.201-205
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• 2009

In an ongoing investigation into anti-inflammatory compounds from natural products, the $CH_2Cl_2$ soluble fraction of Patrinia scabiosaefolia F. (Valerianaceae) was found to inhibit IL-6 production in TNF-$\alpha$ stimulated MG-63 cell line. By means of a bioassay-directed chromatographic separation technique, lappaol E (1), and nortrachelogenin (2) were isolated. These compounds have been isolated from this plant for the first time. Compounds $1{\sim}2$ showed potent antioxidative activity using NBT superoxide scavenging assay. Moreover, compound 1 decreased IL-6 production in TNF-$\alpha$ stimulated MG-63 cell line.

• 한국식품영양과학회지
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• 제28권4호
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• pp.917-923
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• 1999

개불 당단백질의 sarcom 180 cell 고형암 성장 저지능은 4mg/kg에서 43.63%로 나타났으나, 20mg/kg이상의 농도에서는 고형암 성장 저지능을 보이지 않았다. Sarcom 180 cell에 대한 수명 연장 실험에서도 2mg/kg와 4mg/kg의 저농도에서는 상당한 수명연장 효과를 보였으나, 2Omg/kg 이상의 고농도 투여군에서는 수명연장 효과를 나타내지 않았다. 개불 당단백질의 sarcoma 180 cell에 대한 직접적인 세포 독성 효과는 농도의 증가에 따라 약간의 종양세포 살해효과를 보였으나, 그 효과는 미약하였다. 개불 당단백질의 면역능 증진효과는 체중에 대한 간과 비장의 중량비 증가, 총 복강세포수의 증가 그리고, 당단백질을 고농도 투여시 30.78%~46.30%정도의 백혈구 수 증가 등으로 확인되었다. 이러한 결과로서 개불에서 추출한 당단백질의 항암효과는 면역활성의 증진과 면역자극의 효과라고 볼 수 있었으나, 전반적인 면역활성의 증가에도 불구하고, sarcoma 180 cell에 대한 고형암 성장 저지실험과 수명연장실험에서 고농도로 투여시에는 오히려 활성이 나타나지 않았다. 따라서 개불 당단백질의 최적투여 농도와 고농도 투여시의 부작용에 관한 더 많은 연구가 필요하였다. 이상의 결과에서 개불에서 추출한 당단백질의 면역자극에 의한 항종양 활성을 확인 되므로, 일반적으로 사용되어지는 항암 화학요법제와 더 불어 병행될 수 있는 면역증진제로서의 개발 가능성이 있다고 보여진다.

• 한국식품영양과학회지
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• 제39권6호
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• pp.864-871
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• 2010

흰 마늘/흑 마늘 첨가에 따른 김치 젖산균을 이용한 발효물을 제조하여 품질 및 기능성 평가를 수행하였다. 고형분 함량은 17% 흰 마늘 습식분쇄물과 22.63% 흑 마늘 습식분쇄물이다. 마늘 혼합물에 Leuconostoc mesenteroides 균주를 이용하여 24시간 동안 젖산 발효시킨 발효물의 생균수와 점도는 흑 마늘의 첨가농도가 증가함에 따라 감소하였으며, 흑 마늘을 10% 수준으로 첨가한 경우에 생균수는 $1.6\times10^{11}$ cfu/mL, 점도는 9.31 $Pa{\cdot}s^n$를 나타내었다. 마늘 발효물의 물과 70% EtOH 추출물의 polyphenol 함량은 각각 6.29 mg/mL, 5.99 mg/mL이며, DPPH 소거 활성은 $IC_{50}$ 값이 1.03 mg/mL, 1.07 mg/mL, ACE 저해 활성의 $IC_{50}$값은 68.54 mg/mL과 72.78 mg/mL이다. ACE 저해활성은 흑 마늘이 많이 첨가될수록 높게 나타났으며 그중에서 70% EtOH 추출보다 물 추출에서 높은 활성을 나타내는 것으로 나타났다. 마늘 발효물의 sucrose 전환율은 흑 마늘 첨가 농도가 낮을수록 급격하게 증가하면서 흑 마늘 20%, 10%, 0% 첨가에서 각각 20%, 65%, 85%를 보였다. 흑 마늘 10% 함유 발효물의 저장 중 점도는 완만하게 감소하였으며, 생균수는 냉장저장 1주 후 $1.6\times10^{11}$ cfu/mL에서 $6.5\times10^{10}$ cfu/mL로 감소하였으며, 냉동저장이 냉장저장보다 높은 생균수를 나타내었다.