• 환경생물
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• 제22권1호
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• pp.177-183
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• 2004

유기주석화합물인 tributyltin chloride (TBTC), tributyltin oxide (TBTO)와 triphenyltin chloride (TPTC)를 넙치 간장으로 만든 미크로좀에 in vitro적으로 노출시켜서 이들 화합물의 대사에 관여하는 mixed function oxidase (MFO) 중 cytochrome P450 (CYP) 농도와 7-ethoxyresorufin deethylase (EROD) 활성의 변화를 조사하였으며, 또한 in vivo 실험에서는 TPTC를 넙치에게 복강주사(7.5 mg $kg^{-1}$ BW)하여 간장의 MFO (CYP농도, NADPH cytochrome c 환원효소 활성, NADH chtochrome b5 환원 효소 활성, EROD 활성) 반응을 경시적으로 조사하였다. 그 결과, in vitro에서는 TBTC, TBTO 및 TPTC가 모두 CYP 농도와 EROD 활성을 저해하였으며, 저해력은 TPTC가 가장 컸고 이어서 TBTO, TBTC의 순이었다. 유기주석화합물의 노출농도와 노출시간과 비례하면서 저해정도가 커졌으며, 특히 EROD활성의 저해는 노출농도에 크게 의존적이었다. 그리고 in vivo실험에서도 유기주석 화합물은 CYP농도, NADPH cytochrome c 환원효소 활성, NADH cytochrome b5 환원효소 활성, EROD 활성을 억제하였다. EROD 활성은 오염물질에 의한 반응이 민감하고 재현성도 있어 바람직한 측정지표로 이용될 수가 있을 것이다.

• 유기물자원화
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• 제27권4호
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• pp.5-13
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• 2019

본 연구는 음식물류 폐기물 건조분말(FWP)이 처리된 작물의 생육 효과와 FWP와 유기질비료(OF) 혼합물(MFOs)의 이화학적 특성 및 작물생육효과를 평가하기 위해 수행되었다. 3.11%의 NaCl을 함유하는 FWP를 250, 500 및 1,000 kg/10a 씩 상추와 배추에 처리하였을 때, 작물의 엽수, 엽장, 엽폭, 생물중 및 건물중 등과 같은 작물의 생육지수는 대조구보다 감소하였다. MFOs의 이화학적 특성 분석에서 FWP의 배합비율이 증가할수록 MFOs의 질소 및 인산 함량은 감소하였고, 염분함량은 증가하였다. 상추와 배추에 MFOs와 OF를 각각 처리한 후 작물의 건물중으로 생육을 평가할 때, 상추에서는 FWP 10~30% 범위에서, 배추는 FWP 10% 범위에서 유기질비료처리구(대조구)와 유사한 생육을 나타내었다.

• 한국식품영양과학회지
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• 제29권1호
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• pp.168-173
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• 2000

The purpose of this study was to investigate the changes of microsomal mixed function oxidase (MFO) and xanthine oxidase activities (XOD) in the liver of rats exposed to microwave. Sprague-Dawley male rats weighing 200$\pm$10g body weight were randomly assigned to a normal and microwave exposed(MW) groups; microwave exposed groups were divided into two groups; microwave (MW) group and green tea(GT) gropu which were fed distilled water and green tea extracts during experimental periods, respectively. Rats were irradiated with microwave at the frequency of 2.45 GHz for 15min and rats wre sacrificed at the 4th day of the microwave irradiaton. The hemoglobin level of GT group was higher than that of the normal gropu and MW group, but the hematocrit value was not significantly different among all experimental gropus. The activity of serum GOT of MW group was significantly increased but that of GT group was similar to normal group. Activities of GPT were not significantly different among all experimental groups. Liver XOD activity was significantly increased in the microwave exposed groups but green tea normalized the XOD activity. The activity of hepatic microsomal cytochrome P450 was significantly increased in MW group compared to normal group and that of GT group was similar to that of the normal group. The activity of hepatic microsomal NADPH-cytochrome P450 reductase was also significantly increased in MW group compared to normal group, but that of GT group was similar to that fo the normal group. In conclusion, the activities of MFO and XOD were elevated by microwave irradiaton, but the activation of MFO system as well as the damage of the liver by microwave were reduced by green tea supplementation.

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2001년도 학술대회
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• pp.121-122
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• 2001

• 한국환경생물학회:학술대회논문집
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• 한국환경생물학회 2001년도 학술대회
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• pp.119-120
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• 2001

• 한국양식학회:학술대회논문집
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• 한국양식학회 2004년도 수산관련학회 공동학술대회 발표요지집
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• pp.260-261
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• 2004

• 한국양식학회:학술대회논문집
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• 한국양식학회 2004년도 수산관련학회 공동학술대회 발표요지집
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• pp.258-259
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• 2004

• 환경생물
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• 제24권2호
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• pp.195-200
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• 2006

The response of hepatic mixed function oxygenase (MFO) system was investigated in olive flounder exposed to formalin. Hepatic microsome of olive flounder incubated in vitro with formalin demonstrated the induction of cytochrome P450 (CYP), ethoxyresorufin deethylase (EROD), cytochrome P450 reductase (P450R) and cytochrome b5 reductase (b5R) activity. In addition, olive flounder was exposed to 100, 300 and 500 ppm of formalin for 1 h and then transferred to a flow-through type of 1000 L aquarium. Hepatic MFO enzyme activity was determined for 72 h. As the result, hepatic CYP, P450R and EROD activities increased following exposure of formalin, but b5R and GST showed no significant change. These results imply that CYP and P450R can be considered as main hepatic enzymes involving in detoxification of formalin.

• 한국수산과학회지
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• 제36권6호
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• pp.573-577
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• 2003

Effects of 4-nonylphenol (4-NP) on mixed function oxygenase (MFO) in the kidneies of rockfish (Sebastes schlegeli) were investigated. The cytochrome P45O (CYP) contents and NADPH cytochrome P450 reductase, NADH cytochrome b5 reductase and 7-ethoxyredorufin-O-deethylase (EROD) activities of microsome, glutathione S-transferase (GST) activity of cytosol in rockfish exposed to 4-NP for 7 days using an intraperitoneal injection (25 mg/kg) were quantitatively determined. The GST activity of rockfish exposed to 4-NP were higher, up to 5.2 times higher, than those in the control fish. The activities of NADPH-and NADH-dependent reductases were inhibited. On the other hand, CYP contents and EROD activity of the 4-NP exposed fish demonstrated neither an increasing or decreasing trend.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제17권4호
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• pp.1100-1122
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• 2023

Scheduling user-submitted cloud tasks to the appropriate virtual machine (VM) in cloud computing is critical for cloud providers. However, as the demand for cloud resources from user tasks continues to grow, current evolutionary algorithms (EAs) cannot satisfy the optimal solution of large-scale cloud task scheduling problems. In this paper, we first construct a large- scale multi-objective cloud task problem considering the time and cost functions. Second, a multi-objective optimization algorithm based on multi-factor optimization (MFO) is proposed to solve the established problem. This algorithm solves by decomposing the large-scale optimization problem into multiple optimization subproblems. This reduces the computational burden of the algorithm. Later, the introduction of the MFO strategy provides the algorithm with a parallel evolutionary paradigm for multiple subpopulations of implicit knowledge transfer. Finally, simulation experiments and comparisons are performed on a large-scale task scheduling test set on the CloudSim platform. Experimental results show that our algorithm can obtain the best scheduling solution while maintaining good results of the objective function compared with other optimization algorithms.