• 생명과학회지
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• 제19권6호
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• pp.793-798
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• 2009

본 연구는 제주마에서 빈번하게 관찰되는 전신성 백모색 발생의 유전 양상과 유전적 변이와의 상관을 구명하기 위해 수행하였다. 백모색은 표현형과 MC1R 유전자형 분석 자료의 조합을 근거로 결정한 가라, 유마, 적다 등 모든 기본 모색에서 관찰되었다. 제주마에서는 타 품종들에서 KIT 유전자의 이형 접합성에 의해 발생하는 선천성 백색에 대한 잠재적 돌연변이 들은 발견되지 않았다. STX17 유전자의 intron 6 에서 4.6-kb 중복을 보유한 개체들에서 특이적으로 탈색된 백모색이 관찰되었다. 관찰기록과 STX17 유전자형에 따라 제주마에서 관찰되는 탈색된 백화현상은 총마(점진적 백화증, Gray) 로 확인되었다. 가계도 분석에서 총마 형질은 상동염색체성 우성유전형 질로 나타났으며 상동염색체성 열성형질인 albinism과도 구분되었다. 제주마에서 총마 모색이 자마 시기에는 명확하게 발현되는 않으며, 종종 다른 표현형들과 혼동을 일으키기도 하기 때문에, 총마와 이와 유사한 표현형으로 출생 시부터 혼합 모색을 나타내는 조모색, 상처 치료 후 백화, 백반 유사피부 백색증 등에 대한 추가 연구가 요구된다고 하겠다. 그럼에도 불구하고 총마와 유전적 배경의 관계를 구명한 본 연구결과는 제주마에서 분자육종을 위한 유용한 정보를 제공할 것으로 사료된다.

• Journal of Korean Neurosurgical Society
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• 제49권1호
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• pp.13-19
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• 2011

Objective: The purpose of this study is to investigate the combined effects of ginkgo biloba extract, ginkgolide A and B and aspirin on SK-N-MC, human neuroblastoma cell viability and mRNA expression of growth associated protein43 (GAP43), Microtubule-associated protein 2 (MAP2), B-cell lymphoma2 (Bcl2) and protein53 (p53) gene in hypoxia and reperfusion condition. Methods: SK-N-MC cells were cultured with Dulbecco's Modified Eagle's Medium (DMEM) media in $37^{\circ}C$, 5% $CO_2$ incubator. The cells were cultured for 8 hours in non-glucose media and hypoxic condition and for 12 hours in normal media and $O_2$ concentration. Cell survival rate was measured with Cell Counting Kit-8 (CCK-8) reagent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate mRNA levels of GAP43, MAP2, Bcl2, and p53 genes. Results: The ginkgolide A and B increased viable cell number decreased in hypoxic and reperfused condition. The co-treatment of ginkgolide B with aspirin also increased the number of viable cells, however, there was no additive effect. Although there was no increase of mRNA expression of GAP43, MAP2, and Bcl2 in SK-N-MC cells with individual treatment of ginkgolide A, B or aspirin in hypoxic and reperfused condition, the co-treatment of ginkgolide A or B with aspirin significantly increased GAP43 and Bcl2 mRNA levels. In MAP2, only the co-treatment of ginkgolide A and aspirin showed increasing effect. The mRNA expression of p53 had no change in all treating conditions. Conclusion: This study suggests that the combined treatments of Ginkgo biloba extracts and aspirin increase the regeneration of neuroblastoma cells injured by hypoxia and reperfusion.

• 드라이브 ㆍ 컨트롤
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• 제21권1호
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• pp.31-37
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• 2024

In this study, to prevent accidents in underground facilities during excavation, we developed a Lv.3 automated control system that can be configured as an electronic control system without changing the existing hydraulic system in a general excavator and utilized digital map information of underground facilities. We aimed to develop a strategy to prevent accidents caused by operator error. To implement this, a real-time excavator bucket end position recognition and control system was developed through angle measurement of the boom, arm, and bucket using an electronic joystick, RTK-GPS, and angle sensors. In addition, excavators are large, machine-based equipment, and it is difficult to control overshoot due to inertia with feedback control using position recognition information of the bucket tip. Therefore, feed-forward control is used to calculate the moving speed of the bucket tip in real-time to determine the target position. We developed a technology that can converge and verified the performance of the developed system through actual vehicle installation and field tests.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1987년도 한국자동제어학술회의논문집; 한국과학기술대학, 충남; 16-17 Oct. 1987
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• pp.10-13
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• 1987

The main work of this paper are the manufacture of six degree of freedom industrial robot control hardware of 16 bit CPU and the development of five motion control software. The work would draw on KIT of Robotics Laboratory whose extensive experience in these areas; in particular the 68000 assembler and Modula-2 languages, and existing robot control systems. We found that this controller is good for the robot controller of PID types. But, for the use of self-tuning algorithms and real time calculations we need 32 bit CPU robot controller such as MC 68020 microprocessor.

• 한국환경생태학회지
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• 제25권5호
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• pp.658-665
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• 2011

제주도에서는 절멸된 것으로 간주되었던 멧돼지들이 최근 한라산 인근지역에서 발견되었다. 본 연구는 분자유전학적 실험기법을 바탕으로 한라산 멧돼지들이 가축돼지들과 이종교배된 것들인지를 조사하였다. 또한 동일 종내에서의 유전적 유연관계와 분자 성판별을 시험하였다. 가축돼지 품종들(Landrace, Large White, Berkshire, Hampshire, Duroc)과의 교배여부는 핵 DNA와 미토콘드리아 DNA에서 4 종류의 분자 표지인자(MC1R, KIT, 조절영역, ND2)를 적용하여 시험하였다. 야생멧돼지 집단의 모든 개체들이 동일한 mtDNA 조절영역 서열을 나타내었고, 그 서열들은 중국 동북부 재래돼지들과 동일하였으나 기존에 보고된 한반도 멧돼지의 서열들과는 다른 것으로 확인되었다. 이상의 연구결과는 한라산 멧돼지집단이 중국 재래돼지 품종들과 근연이면서, 기존에 연구되지 않았던 유전적 계통에서 유래한 것으로 사료된다. 분자 성판별 결과 수컷에 비해 암컷이 2 배 이상으로 확인되어, 한라산 야생멧돼지 집단이 팽창하고 있으며, 조절하지 않으면 집단 규모는 극적으로 증가할 것이다.

• Restorative Dentistry and Endodontics
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• 제44권2호
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• pp.17.1-17.10
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• 2019

Objectives: Root resorption is an unexpected complication after replantation procedures. Combining anti-osteoclastic medicaments with retrograde root filling materials may avert this resorptive activity. The purpose of this study was to assess effects of a cathepsin K inhibitor with calcium silicate-based cements on osteoclastic activity. Methods: MC3T3-E1 cells were cultured for biocompatibility analyses. RAW 264.7 cells were cultured in the presence of the receptor activator of nuclear factor-kappa B and lipopolysaccharide, followed by treatment with Biodentine (BIOD) or ProRoot MTA with or without medicaments (Odanacatib [ODN], a cathepsin inhibitor and alendronate, a bisphosphonate). After drug treatment, the cell counting kit-8 assay and Alizarin red staining were performed to evaluate biocompatibility in MC3T3-E1 cells. Reverse-transcription polymerase chain reaction, tartrate-resistant acid phosphatase (TRAP) staining and enzyme-linked immunosorbent assays were performed in RAW 264.7 cells to determine the expression levels of inflammatory cytokines, interleukin $(IL)-1{\beta}$, IL-6, tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and prostaglandin E2 (PGE2). Data were analyzed by one-way analysis of variance and Tukey's post hoc test (p < 0.05). Results: Biocompatibility results showed that there were no significant differences among any of the groups. RAW 264.7 cells treated with BIOD and ODN showed the lowest levels of $TNF-{\alpha}$ and PGE2. Treatments with BIOD + ODN were more potent suppressors of inflammatory cytokine expression (p < 0.05). Conclusion: The cathepsin K inhibitor with calcium silicate-based cement inhibits osteoclastic activity. This may have clinical application in preventing inflammatory root resorption in replanted teeth.

• 대한임상검사과학회지
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• 제45권4호
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• pp.149-153
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• 2013

Candidiasis is a fungal infection of the most common causes; generally, opportunistic infections occur often in patients with weakened immune systems. Because of high rates in fungal infection patients and increasing frequency of being isolated from clinical materials, quickly identifying of Candida albicans is critical. By identifying 404 yeast cell strains of referred samples via API 20C kits, NGL and PRO tests and Germ tube (GT) test were conducted and compared. In the 3.0 McFarland yeast cells, 0.1% ${\rho}-nitrophenyl-N-acetyl-{\beta}-D-galactosaminide$ (NGL) and 0.04% ${\small{L}}$-proline ${\beta}$-naphtylamide (PRO) were each put in test tubes and incubated at $35^{\circ}C$ for 15, 30, 60 and 90 minutes. Afterwards, 1 drop of 2% NaOH was applied, and if the color turned yellow; it was positive for NGL test. Afterwards, 1% ${\rho}$-dimethylaminocinnamaldehyde was applied, and if the upper layer turned pink or red, it was positive for PRO test. NGL and PRO tests were conducted for all C. albicans and identified accurately within 30 minutes. In NGL, PRO test, false-positive, negative were not seen, whereas, GT test showed false-positive in 1 strain and false-negative in 3 strains. Therefore, sensitivity and specificity of NGL, PRO tests were 100% and 99.5%, respectively, and positive and negative predictive rate were 99.5% and 100%, respectively. However, GT test sensitivity and specificity were 98.5% and 99.5%, respectively, and positive and negative predictive rates were 99.5% and 98.5%, respectively. In conclusion, NGL, PRO tests are better than GT tests for sensitivity and specificity, therefore, these reliable tests will be useful in clinical laboratories.

• 한국언론정보학보
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• 제27권
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• pp.183-212
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• 2004

이 연구는 맥루한의 '인간 확장' 개념으로부터 출발해, 이동전화가 매개하고 있는 인간 커뮤니케이션 확장 개념이 어떤 차원들로 구성되어 있는지를 논증해보고, 이들 각 차원의 상호작용에 의한 구체적인 이동전화 이용행태와 사회적 관계의 확장의 양상을 실증적 자료를 바탕으로 검증하고자 했다. '이동성'과 '개별성'이라는 이동전화의 매체적 특성은 인간 커뮤니케이션의 '기능 확장'과 '자아 확장'이라는 두 개의 차원 흑은 영역을 견인하고 있으며, 이는 이동전화의 도구적 이용동기와 표현적 이용동기에 각각 조응하고 있음을 밝혀 보았다. 나아가 설문조사방법을 통해 수집된 자료를 분석한 결과, 이용동기의 두 차원의 상호작용 결과, 이동전화 이용자들의 이용행태와 사회적 관계의 확장은 다양한 양상으로 전개되는 것으로 나타났다. 결론적으로 이동전화가 견인하고 있는 인간 커뮤니케이션의 확장은 일률적인 내용과 방향으로 전개되는 것이 아니라, 확장 개념의 두 차원이 상호작용함으로써 불균등한 양상으로 진행되는 것을 알 수 있었다.

• Archives of Plastic Surgery
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• 제37권5호
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• pp.531-534
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• 2010

Purpose: Lipobean$^{(R)}$s, widely used in lipodissolving techniques, contain phosphatidylcholine and sodium deoxycholate as its main substances. They have been approved only as medication for liver disease by the FDA. However, they have been used under various clinical settings without exact knowledge of its action mechanism. The authors designed an in vitro study to analyze the effects of different concentrations of phosphatidylcholine and sodium deoxycholate on adipocytes and other types of cells. Methods: Human adipose-derived stem cell were cultured and induced to differentiate into adipocytes. Fibroblasts extracted from human inferior turbinate tissue, and MC3T3-E1 osteoblast lines were cultured. Phosphatidylcholine solution dissolved with ethanol was applied to the culture medium at differing concentrations (1, 4, 7, 10 mg/mL). The sodium deoxycholate solution dissolved in DMSO applied to the medium at differing concentrations (0.07, 0.1. 0.4. 0.7 mg/mL). Cells were dispersed at a concentration of $5{\times}10^3$ cells/well in 24 well plates, and surviving cells were calculated 1 day after the application using a CCK-8 kit. Results: The number of surviving cells of adipocytes, fibroblasts and osteoblasts decreased as the concentration of sodium deoxycholate increased. However, all types of cells that had been processed in a phosphatidylcholine showed a cell survival rate of over 70% at all concentrations. Conclusion: This study shows that sodium deoxycholate is the more major factor in destroying adipocytes, and it is also toxic to the other cells. Therefore, we conclude that care must be taken when using Lipobean$^{(R)}$s as a method of reducing adipose tissue, for its toxicity may destroy other nontarget cells existing in the subcutaneous tissue layer.

• 한국균학회지
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• 제38권2호
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• pp.146-151
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• 2010

검체는 2005년 1월1일부터 2009년 12월31일까지 C 병원 진단 검사의학과에 의뢰된 환자의 임상검체 총 366,661예로부터 총 167,955 미생물이 분리되었고 이 중 6,517주의 Candida spp. 분리균을 자동화기기로 동정한 것을 후향적으로 처리 하였다. 배양균주는 smooth하고, 크림색이며, 약간 mucoid하고, 혼탁한 집락을 0.45% salin에 McFarland No 1.6-2.0되게 탁도를 맞춘 후 반응시약이 들어 있는 ID-YST card에 접종하여, VITEK II(bioMerieux Duham NC, USA)의 자동화기기에 넣어 동정하였고, 그 결과는 다음과 같다. Candida spp.의 분리율은 2005년도에 비하여 2009년이 1.9배 증가하였다. 분리빈도가 높은 Candida spp.는 C. albicans 56.4%, C. tropicalis 17.7%, C. glabrata 10.7%, C. parapsilosis 9.5%였고, Candida spp가 10%이상 분리된 검체는 객담 30.1%, 무작위소변 25.0%, 혈액 15.8%, 카테타소변 13.5%였으며, 7%이상 분리된 부서는 호흡기내과 20.5%, 신장내과 11.0%, 감염내과 10.4%, 응급의학과 10.0%, 혈액종양내과 9.6%, 외과 7.5%, 소화기내과 7.4%였다. Candida spp가 증가된 달은 7월이 10.6%였고, 가장 낮게 분리된 달은 2월이 6.1%였다. Candida spp.가 증가된 연령군은 70세 이상 군이 40.1%였고, 가장 낮게 분리된 연령군은 10대가 1.3%였고, 0-49세군(1.3-7.5%)대 50세 이상 군(16.7-40.1%)의 비교에서는 1 : 4.7로 50세 이상 군이 월등히 높았다.