• 한국버섯학회지
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• 제15권3호
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• pp.111-117
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• 2017

본 연구는 밀리타리스 동충하초의 교배형 유전자인 MAT1-1과 MAT1-2 두 종류에 특이적인 프라이머를 사용하여 multiplex PCR을 실시하였고, 그 결과 형성된 MAT1-1과 MAT1-2에 대한 233-bp와 191-bp에서 DNA 밴드를 통해 교배형 및 교배여부를 확인하였고 자실체 특성검정을 통해 품종특성이 우수한 새로운 동충하초 '도원홍초 2호'를 개발하였다. 신품종 '도원홍초 2호'의 자실체는 곤봉형이고 밝은 주황색을 띠었으며, 코디세핀 함량은 0.33%였고 자좌의 굵기와 길이는 각각 3.5 mm와 7.1 cm였다. '도원홍초'와 비교할 때, 새로운 '도원홍초 2호'의 수량은 7%가 증수되었고 경도가 높은 특징을 보였다. 균사생장의 적온은 $20{\sim}25^{\circ}C$였고 버섯 발생의 적온은 $18{\sim}22^{\circ}C$였으며, 접종에서부터 자실체 발생까지의 기간은 49.7일이 소요되었다. 신품종 '도원홍초2호'는 교배형 분자마커를 육종과정에 이용하여 효율성을 높였으며, 우수한 재배적 특성으로 동충하초 인공재배 및 산업적 생산에 기여할 것으로 판단된다.

• 한국균학회지
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• 제28권1호
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• pp.22-25
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• 2000

Cosmid clone으로부터 mating activity를 가지는 pSC13의 양 말단은 1-71 $A{\alpha}3$ 교배유전자좌 Z3부위 2,430 bp와 Y3 region 8,160 bp 부근에서 homology를 지니는 것으로 나타나 교배활성에 필요한 모든 부위를 함유하는 것으로 사료되었다. 그리고 pSCE2는 1-71 $A{\alpha}3$의 Y region의 6,080 bp 부근에서 거의 완전한 homology를 가지는 것으로 보아 $A{\alpha}3$의 Y region을 함유하고 있음을 알 수 있으며, 또한 pSCE1도 MEP의 일부 sequence와도 거의 완전한 homology를 가지는 것으로 나타나 1-71 $A{\alpha}3$ 교배유전자좌와 모두 유사한 염기서열를 모두 지니면서 북미자생 치마버섯의 교배유전자좌의 염기배열이 남미자생의 것과 거의 유사한 분자적 구조를 지니고 있음을 나타내었다. 결정된 DNA sequence는 3265 bp로서 남미산 치마버섯 1-71 stain의 mating활성을 나타내는 $A{\alpha}3$ locus 염기서열중에 Z region과 거의 완전한 약 96%의 homology를 나타내었다. 또한 Polypeptide sequence비교에서도 약 82%의 높은 homology를 나타내었으며, 특히 transcription regulator로 알려진 homeodomain 및 acidic region에서는 각각 약 74%. 82%의 상당히 높은 비율의 homology를 지니고 있음이 확인되었다. 이러한 결과로 보아 남미와 북미의 대륙간에 자생하는 같은 allele type간에도 상당히 높은 비율의 교배유전자좌의 보존이 이루어지고 있음을 알 수 있다.

• 한국응용곤충학회지
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• 제46권2호
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• pp.269-274
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• 2007

본 연구는 복숭아순나방(Grapholita molesta (Busck))의 상용 교미교란제가 사과원에서 미치는 교미교란 효과를 분석하기 위해 먹이트랩으로 암컷을 채집하여 교미율을 결정하였다. 복숭아순나방 월동집단을 대상으로 세 종류의 교미교란제가 비교되었으며, 교미교란 효과는 수컷 유인력 감소, 신초피해 및 암컷 교미율을 상호 비교하였다. 야외 암컷은 테르피닐아세테이트 트랩에 수컷과 함께 유인되었다. 수컷의 정자주머니는 암컷의 교미낭과 유사한 크기를 가졌다. 교미교란제가 처리된 지역은 95% 이상의 수컷 유인력 저하를 나타냈으나, 사과의 신초 피해가 발생하였다. 한편 교미교란제가 처리된 지역에서 채집된 암컷의 교미율은 약 35% 이상을 나타내었다. 본 연구는 교미교란제 효능 분석에 수컷의 유인력 감소보다는 암컷의 교미율 판정이 보다 교미교란 효과를 작물피해로 연결시키는데 의미가 있다는 것을 보여주었다.

• 약학회지
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• 제46권6호
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• pp.452-458
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• 2002

Dehydroevodiamine-HCl (DHED), which is a component separated from Evodia rutaecarpa Bentham, has novel anticholinesterase and antiamnesic activities in a scopolamine-induced amnesia model. Several studies suggest that DHED might be an effective drug for Alzheimer's disease and a vascular type of dementia. DHED was at dose levels of 0, 50, 100 and 200 mg/kg/day administered intraperitoneally to Sprague-Dawley male rats for 60 days before mating and to females from 14 days before mating to 7 days after mating. Effects of the DHED on general symptom and reproductive performance of parent animals and embryonic development were examined. In male parents, whereas no death was observed, reduction in the increase rate of body weight was found at 200 mg/kg. In female parents, both of the mating performance and the fertility of parent animals were decreased at 200 mg/kg, but not significantly. In 200 mg/kg treated group, the fetal death rate was increased but total fetuses showed no changes compared to the control group. There were no malformed F1 fetuses in all groups.

• 한국버섯학회지
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• 제10권3호
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• pp.101-108
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• 2012

Pleurotus ostreatus 'Miso' is a mutant strain showing white color in pileus from the known parent strain 'Wonhyeong 1'. Shape and several other characters also vary with culture conditions. Mating experiments were performed to understand interstrain mating relationship using monokaryons of the parent and the mutant strains. All monokaryons were grown from single spores isolated from freshly collected fruit bodies. Pairings were performed in 90 mm petri dishes on PDA. They were allowed to grow at 25 until two fronts of the advancing mycelia met and developed a conspicuous contact zone. The contact zone and the outer edges of paired colonies on each plate were examined for clamp connections. The parent and the mutant resulted in tetrapolar incompatibility in intrastrain crosses. In interstrain crosses, each monokaryotic tester strain of the parent strain was out-crossed to monokaryotic tester strains of the mutant. As a result of these crosses it was found that both strains share the same A and B incompatibility factors yielding 25% compatibility.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.115-123
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• 2003

Oviposition and egg laying is an important physiological and behavioural event in the life cycle of the silkworm, Bombyx mori (L). Oviposition and egg laying is dependent on a number of intrinsic and extrinsic factors viz., neural, hormonal, environmental, physical, behavioral etc for the perpetuation of population. Although, the virgin female moths have fully developed embryos but active egg laying begins under the influence of mating which provides essential copulation stimulus for oviposition. After mating drastic biochemical changes occurred that incites egg laying under the influence of optimum environmental conditions. Weight of pupae as well as larval density has significant role on oviposition and egg laying behaviour in the silkworm wherein high pupal weight and inadequate rearing space affects not only the biology, morphology and physiology but also the oviposition and egg laying. Surface topography, plane of inclination, mating length, age of male moth, temperature and photoperiod etc has significant effect on reproductive physiology of silkworm. An attempt has been made in this review article to elucidate briefly the works carried out on mating behaviour, direct and indirect copulation stimulus, vitellogenesis, influence of environmental factors besides effect of weight of pupae and or pharate adult and larval density on oviposition and egg laying behavior in the silkworm, B. mori and its significance in silkworm seed production.

• 대한의생명과학회지
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• 제19권3호
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• pp.213-223
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• 2013

The objectives of this study are to develop a molecular diagnosis to differentiate serotypes and mating-types of C. neoformans/C. gattii complex isolates from pigeon droppings in Korea and to elucidate molecular epidemiology of the isolates. Phenotypes and genotypes of C. neoformans/C. gattii complex isolates were identified by biochemical properties and PCR using specific CNLAC1 gene, respectively. To classify serotypes and mating-types of C. neoformans/C. gattii complex isolates, the five reference strains and thirty-three isolates in Korea were investigated by restriction fragment length polymorphism (RFLP) analysis using CNLAC1 gene for varieties, by random amplified polymorphic DNA (RAPD) for serotyping, and by PCR using specific primer sets for mating typing. All isolates in Korea were belonged to C. neoformans var. grubii (serotype A) by RFLP and RAPD patterns which showed high sensitivity and specificity. Therefore, RFLP and RFLP were available to differentiate varieties and serotypes of C. neoformans. Amplification patterns of the five reference strains by specific PCR for mating typing were differentiable, and all isolates were classified into $MAT{\alpha}$. All C. neoformans environmental isolates in Korea were Cr. neoformans serotype A and $MAT{\alpha}$ which is a more virulent pathogen. This study suggests that RFLP and RAPD are rapid and correct molecular diagnosis tools for epidemiology of C. neoformans/C. gattii complex isolates.

• 대한기계학회논문집
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• 제12권4호
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• pp.747-754
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• 1988

본 연구에서는 우리나라 자동차업계에서 널리 사용되는 복합조직인 고장력 강 판(high strength steel sheet, HS)과 아연도금강판(galvanized steel sheet, GA)을 선택하여 각 강판간의 점용접 피로특성을 규명하기 위하여 아래와 같은 4가지 조건하 의 단점 점용접 시험편을 제작하였다.

• 대한기계학회논문집
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• 제13권1호
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• pp.48-58
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• 1989

이 논문의 목적은 사용자가 부품간의 연결정보로는 단지 만남조건 (mating condition)만을 입력함으로써 조립체가 생성이 되고 또 그로부터 동역학 또는 기구학적 해석 프그램과의 연결을 위한 정보가 유도되는 조립체 모델링 시스템(assembly modeling system)을 개발하는 것이다. 만남조건을 통해 조립체를 생성하는 방법은 이미 Rocheleau와 Lee에 의해 개발되었으나 그들은 주로 각 부품의 위치를 찾아내는 데에만 관심을 두었고 본 논문에서는 그것의 확장으로 해석 프로그램과 연결시키는데 필요한 정보의 유도를 추가로 고려하였다.

• 대한수의학회지
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• 제15권2호
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• pp.215-222
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• 1975

Infertility and breeding status of Korean cows which are reared in a extensive alpine ranches of Daegwanryeong area was investigated. The results obtained were summarized as follows, 1. Infertility frequency of the cows was 25.9% against a total examined number of 675 heads, and was 17.6% against a total rearing number. 2. The distributions of infertile cows 44.6% of Granular vaginitis-like syndrome, 36% of repeat breeder, 8.6% of symptom of ovary, 6.8% of symptom of uterus and 4% of other symptoms. 3. According to its age were found by 51.4% upon 2 years of age, 30.3% upon 3 years of age, 11.4% upon 4 years of age and 6.9% upon S years of age. 4. Breeding status of Korean cows showed 74.1% of conception rate in the cases of pre-treatment, while breeding status increased markedely in the cases of post-treatment by the 85.8%. 5. The conception rate by the artificial insemination mainly on the first mating was 36.1%. on the second mating 23.0% and on the third mating 18.0%. Total conception rate between first and third mating was 77.1%. 6. The therapeutic effect by treatment for granular vaginitis-like syndrome was 87.1%.