• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.136-142
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• 1992

Bacillus stearothemophilus isolated from soil was identified to express multiple extracellular xylanases. Two HindIII restriction fragments of 5.4 and 6.4 kb from B. stearothermophilus genomic DNA were cloned into pBR322 to obtain recombinant plasmids pMG0l and pMG02, respectively, which enabled E. coli HBlOl cells to produce $\beta$-D-xylosidase activity. By subcloning into pUC18 and Southern blotting, the loci of the $\beta$-D-xyiosidase genes were elucidated to be on non-homologous DNA fragments of 2.2 kb from pMGOl(pMG1) and 1.0 kb from pMG02(pMG2), respectively. The two enzymes produced in E. coli cleaved xylobiose, xylotriose, xylotetrose and xylotetrose to produce xylose as a major end product. The gene on pMG1, distinct from that on pMG2 was observed to encode a bifunctional protein that displayed both P-D-xylosidase (EC.3.2.1.37) and a-L-arabinofuranosidase activities (EC.3.2.1.55).

• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.120-122
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• 2007

All living organisms use numerous signal-transduction pathways to sense and respond to their environments and thereby survive and proliferate in a range of biological niches. Molecular dissection of these signalling networks has increased our understanding of these communication processes and provides a platform for therapeutic intervention when these pathways malfunction in disease states, including infection. Owing to the expanding availability of sequenced genomes, a wealth of genetic and molecular tools and the conservation of signalling networks, members of the fungal kingdom serve as excellent model systems for more complex, multicellular organisms. Here, we employed Cryptococcus neoformans as a model system to understand how fungal-signalling circuits operate at the molecular level to sense and respond to a plethora of environmental stresses, including osmoticshock, UV, high temperature, oxidative stress and toxic drugs/metabolites. The stress-activated p38/Hog1 MAPK pathway is structurally conserved in many organisms as diverse as yeast and mammals, but its regulation is uniquely specialized in a majority of clinical Cryptococcus neoformans serotype A and D strains to control differentiation and virulence factor regulation. C. neoformans Hog1 MAPK is controlled by Pbs2 MAPK kinase (MAPKK). The Pbs2-Hog1 MAPK cascade is controlled by the fungal "two-component" system that is composed of a response regulator, Ssk1, and multiple sensor kinases, including two-component.like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. We also identified and characterized the Ssk2 MAPKKK upstream of the MAPKK Pbs2 and the MAPK Hog1 in C. neoformans. The SSK2 gene was identified as a potential component responsible for differential Hog1 regulation between the serotype D sibling f1 strains B3501 and B3502 through comparative analysis of their meiotic map with the meiotic segregation of Hog1-dependent sensitivity to the fungicide fludioxonil. Ssk2 is the only polymorphic component in the Hog1 MAPK module, including two coding sequence changes between the SSK2 alleles in B3501 and B3502 strains. To further support this finding, the SSK2 allele exchange completely swapped Hog1-related phenotypes between B3501 and B3502 strains. In the serotype A strain H99, disruption of the SSK2 gene dramatically enhanced capsule biosynthesis and mating efficiency, similar to pbs2 and hog1 mutations. Furthermore, ssk2, pbs2, and hog1 mutants are all hypersensitive to a variety of stresses and completely resistant to fludioxonil. Taken together, these findings indicate that Ssk2 is the critical interface protein connecting the two-component system and the Pbs2-Hog1 pathway in C. neoformans.

• Investigative Magnetic Resonance Imaging
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• v.15 no.1
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• pp.22-31
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• 2011

Purpose : To compare free-breathing and respiratory-triggered diffusion-weighted imaging on 1.5-T MR system in the detection of hepatic lesions. Materials and Methods: This single-institution study was approved by our institutional review board. Forty-seven patients (mean 57.9 year; M:F = 25:22) underwent hepatic MR imaging on 1.5-T MR system using both free-breathing and respiratory-triggered diffusion-weighted imaging (DWI) at a single examination. Two radiologists retrospectively reviewed respiratory-triggered and free-breathing sets (B50, B400, B800 diffusion weighted images and ADC map) in random order with a time interval of 2 weeks. Liver SNR and lesion-to-liver CNR of DWI were calculated measuring ROI. Results : Total of 62 lesions (53 benign, 9 malignant) that included 32 cysts, 13 hemangiomas, 7 hepatocellular carcinomas (HCCs), 5 eosinophilic infiltration, 2 metastases, 1 eosinophilic abscess, focal nodular hyperplasia, and pseudolipoma of Glisson's capsule were reviewed by two reviewers. Though not reaching statistical significance, the overall lesion sensitivities were increased in respiratory-triggered DWI [reviewer1: reviewer2, 47/62(75.81%):45/62(72.58%)] than free-breathing DWI [44/62(70.97%):41/62(66.13%)]. Especially for smaller than 1 cm hepatic lesions, sensitivity of respiratory-triggered DWI [24/30(80%):21/30(70%)] was superior to free-breathing DWI [17/30(56.7%):15/30(50%)]. The diagnostic accuracy measuring the area under the ROC curve (Az value) of free-breathing and respiratory-triggered DWI was not statistically different. Liver SNR and lesion-to-liver CNR of respiratory-triggered DWI ($87.6{\pm}41.4$, $41.2{\pm}62.5$) were higher than free-breathing DWI ($38.8:{\pm}13.6$, $24.8{\pm}36.8$) (p value < 0.001, respectively). Conclusion: Respiratory-triggered diffusion-weighted MR imaging seemed to be better than free-breathing diffusion-weighted MR imaging on 1.5-T MR system for the detection of smaller than 1 cm lesions by providing high SNR and CNR.

• International Journal of Automotive Technology
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• v.8 no.6
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• pp.675-685
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• 2007

A single cylinder SI engine with a VVA system is modeled by the coupling of a commercial 1D simulation package and an additional combustion model and validated by comparison with experimental data. A number of simulations are carried out to investigate the effects of five different VVA systems on the performance and fuel efficiency of the baseline engine. Finally, the simulation model is applied to an extensive computational study to map out the strategies to operate the engines with dual CVVT and dual CVVT-2 step VVL systems in a fuel-efficient manner.

• Bulletin of the Korean Mathematical Society
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• v.38 no.2
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• pp.347-355
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• 2001

Let ($X, \omega$) be a closed symplectic 4-manifold. Let a finite cyclic group G act semifreely, holomorphically on X as isometries with fixed point set $\Sigma$(may be empty) which is a 2-dimension submanifold. Then there is a smooth structure on the quotient X'=X/G such that the projection $\pi$:X$\rightarrow$X' is a Lipschitz map. Let L$\rightarrow$X be the Spin$^c$ -structure on X pulled back from a Spin$^c$-structure L'$\rightarrow$X' and b_2^$+(X')>1. If the Seiberg-Witten invariant SW(L')$\neq$0 of L' is non-zero and $L=E\bigotimesK^-1\bigotimesE$ then there is a G-invariant pseudo-holomorphic curve u:$C\rightarrowX$,/TEX> such that the image u(C) represents the fundamental class of the Poincare dual $c_1$(E). This is an equivariant version of the Taubes' Theorem.

• YAKHAK HOEJI
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• v.54 no.6
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• pp.461-465
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• 2010

We investigated the role of L-type $Ca^{2+}$ channel in receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclast formation. BayK 8644, a L-type $Ca^{2+}$ channel agonist, was shown to increase the RANKLinduced osteoclastogenesis and actin ring formation in mouse bone marrow-dereived macrophage (BMM) culture system. BayK 8644 stimulated RANKL-induced extracellular signal-regulated kinase (ERK) and p38 MAP kinase (MAPK) activation, which leads to increased nuclear factor of activated T cells (NFAT)c1 expression. Taken together, these data indicate that L-type $Ca^{2+}$ channel regulates osteoclast formation possibly through ERK- and p38-mediated NFATc1 expression.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.10a
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• pp.139-141
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• 2007

Compression tests were carried out to investigate morphologies of compressed specimen, deformation microstructure and stress-strain relation in high-nitrogen austenite stainless steel. Tests were performed under a wide range of temperature and, with true strain rates up to $\dot{\varepsilon}$ =0.05, 0.1, 0.5 and $1.0s^{-1}$. The activation energy of loading force was equal to plastic deformation energy within the temperature range of $900^{\circ}C$ to $1250^{\circ}C$. Dynamically recrystallized grain size decreased with an increasing strain rate and temperature. Flow stresses and deformation microstructures, were used to quantify the critical strain rate and recrystallized grain size. The grain size versus strain rate-temperature map obtained in the study was in good agreement with the deformation microstructures of compressed specimens.

• Bulletin of the Korean Mathematical Society
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• v.57 no.6
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• pp.1409-1426
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• 2020

Let 𝒜_n be the class of analytic functions f(z) of the form f(z) = z + ∑^∞_k=n+1 α_kz^k, n ∈ ℕ defined on the open unit disk 𝔻, and let $${\Omega}_n:=\{f{\in}{\mathcal{A}}_n:\|zf^{\prime}(z)-f(z)\|<{\frac{1}{2}},\;z{\in}{\mathbb{D}}\}$$. In this paper, we make use of differential subordination technique to obtain sufficient conditions for the class Ω_n. Writing Ω := Ω₁, we obtain inclusion properties of Ω with respect to functions which map 𝔻 onto certain parabolic regions and as a consequence, establish a relation connecting the parabolic starlike class 𝒮_P and the uniformly starlike UST. Various radius problems for the class Ω are considered and the sharpness of the radii estimates is obtained analytically besides graphical illustrations.

• The Journal of Korea Robotics Society
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• v.14 no.4
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• pp.293-300
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• 2019

In this paper, an Embedded solution for fast navigation and precise positioning of mobile robots by floor features is introduced. Most of navigation systems tend to require high-performance computing unit and high quality sensor data. They can produce high accuracy navigation systems but have limited application due to their high cost. The introduced navigation system is designed to be a low cost solution for a wide range of applications such as toys, mobile service robots and education. The key design idea of the system is a simple localization approach using line features of the floor and delayed localization strategy using topological map. It differs from typical navigation approaches which usually use Simultaneous Localization and Mapping (SLAM) technique with high latency localization. This navigation system is implemented on single board Raspberry Pi B+ computer which has 1.4 GHz processor and Redone mobile robot which has maximum speed of 1.1 m/s.

• Bulletin of the Korean Mathematical Society
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• v.56 no.5
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• pp.1297-1314
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• 2019

In this work, we investigate the following fractional boundary value problems $$\{_tD^{\alpha}_T({\mid}_0D^{\alpha}_t(u(t)){\mid}^{p-2}_0D^{\alpha}_tu(t))\\={\nabla}W(t,u(t))+{\lambda}g(t){\mid}u(t){\mid}^{q-2}u(t),\;t{\in}(0,T),\\u(0)=u(T)=0,$$ where ${\nabla}W(t,u)$ is the gradient of W(t, u) at u and $W{\in}C([0,T]{\times}{\mathbb{R}}^n,{\mathbb{R}})$ is homogeneous of degree r, ${\lambda}$ is a positive parameter, $g{\in}C([0,T])$, 1 < r < p < q and ${\frac{1}{p}}<{\alpha}<1$. Using the Fibering map and Nehari manifold, for some positive constant ${\lambda}_0$ such that $0<{\lambda}<{\lambda}_0$, we prove the existence of at least two non-trivial solutions