• Journal of Veterinary Clinics
• /
• v.29 no.1
• /
• pp.27-32
• /
• 2012

The purpose of this study was to evaluate survival rates of vitrified mouse blastocysts in various vitrification solutions (cryoprotectants) and apparatuses. The mouse blastocysts were harvested from culture of mouse 2 cell embryo and were divided into three group (i) untreated (control); (ii) exposed to cryoprotectant agents; or (iii) cryopreserved by various vitrification apparatuses. Vitrification solutions are 40% ethylene glycol (EG) + 5.8 mg/mL ficoll + 0.5M sucrose (EFS solution), 3M glycerol + 3M EG (ES solution), 20% EG + 20% dimethyl sulfoxide (ED solution), 3M EG + 1.0 m sucrose (ES solution). Vitrification apparatuses consisted of 5 groups ; closed plastic straw (CPS), electron microscope (EM) grid, cryoloop, open pulled straw (OPS), and glass micropippete in plastic straw (GPS). The survival rates of control were 88.0%. The survival rates of exposed blastocysts in EFS, GE, ED, and ES solutions were 70.8%, 43.5% (P<0.01), 83.3% and 65.2%, respectively. The survival rates of vitrified blastocysts in CPS, EM grid, cryoloop, OPS and GPS were 56.5% (P< 0.01), 72.7%, 83.3%, 60.9% (P<0.05) and 54.2% (P<0.01), respectively. Among the vitrification solutions, the highest survival rate was seen in blastocysts vitrified in EG + DMSO (83.3%). The survival rate was not significantly different from that of the control (88%). Blastocysts cryopreserved with glycerol in all groups had an overall low survival rate of 43.5%. Survival rate of mouse blastocysts between vitrification apparatuses showed higher in cryoloop.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.14 no.4
• /
• pp.929-934
• /
• 2010

Improvements in characteristics of a single junction AlGaAs/GaAs solar cell are observed by capping a PMMA lens on it. In order to show the effect of the lens, characteristics of a single junction AlGaAs/GaAs solar cell before and after the lens formation are compared under the one-sun illumination condition ($100mW/cm^2$). Characteristics of the solar cell under very weak illumination condition (about 1200 lux) is also measured with the lighting of a fluorescent desk lamp. About 5% of cell efficiency is improved after the capping of PMMA lens on the single junction AlGaAs solar cell and $83\;{\mu}m/cm^2$ of electrical power was generated with the lighting of a desk lamp.

• Biomolecules & Therapeutics
• /
• v.30 no.2
• /
• pp.184-190
• /
• 2022

Targeting the cystine/glutamate exchange transporter, system x_c^-, is a promising anticancer strategy that induces ferroptosis, which is a distinct form of cell death mediated by iron-dependent lipid peroxidation. The concentration of ⳑ-cystine in culture medium is higher than the physiological level. This study was aimed to evaluate the effects of ⳑ-cystine concentration on the efficacy of ferroptosis inducers in hepatocellular carcinoma cells. This study showed that treatment with sulfasalazine or erastin, a system x_c^- inhibitor, decreased the viability of Huh6 and Huh7 cells in a dose-dependent manner, and the degree of growth inhibition was greater in medium containing a physiological ⳑ-cystine concentration of 83 µM than in commercial medium with a concentration of 200 µM ⳑ-cystine. However, RSL3, a glutathione peroxidase 4 inhibitor, decreased cell viability to a similar extent in media containing both ⳑ-cystine concentrations. Sulfasalazine and erastin significantly increased the percentages of propidium iodide-positive cells in media with 83 µM ⳑ-cystine, but not in media with 200 µM ⳑ-cystine. Sulfasalazine- or erastin-induced accumulation of lipid peroxidation as monitored by C11-BODIPY probe was higher in media with 83 µM ⳑ-cystine than in media with 200 µM ⳑ-cystine. In contrast, the changes in the percentages of propidium iodide-positive cells and lipid peroxidation by RSL3 were similar in both media. These results showed that sulfasalazine and erastin, but not RSL3, were efficacious under conditions of physiological ⳑ-cystine concentration, suggesting that medium conditions would be crucial for the design of a bioassay for system x_c^- inhibitors.

• Journal of the Korean Magnetics Society
• /
• v.9 no.6
• /
• pp.278-284
• /
• 1999

The effect of TiO$_2$addition has been studied on the power loss, density, initial permeability, resistivity, and microstructure of Mn-Zn ferrites. TiO$_2$addition increased the density of sintered body and decreased of the initial permeability. Activation energy for electron hopping as well as electrical resistivity increased with TiO$_2$addition. The toroidal core sintered at 1150 $^{\circ}C$ with 1.5 wt% of TiO$_2$demonstrated the power loss of 83 mW/㎤ at 1 MHz, 25 mT, 8$0^{\circ}C$. However, the same specimen sintered at 120$0^{\circ}C$ lead to the power loss, 1168 mW/㎤ at 1 MHz, 25 mT, 8$0^{\circ}C$ and developed an exaggerated grain growth.

• Korean Journal of Mathematics
• /
• v.4 no.2
• /
• pp.83-88
• /
• 1996

In 1994 Z.Liang constructed an iterative method for the solution of nonlinear equations involving m-accretive operators in uniformly smooth Banach spaces. In this paper we apply the slight variants of Liang's iterative methods and generalize the results of Z.Liang. Moreover our proof is more simple than Liang's proof.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.37 no.4
• /
• pp.467-471
• /
• 2010

Amelogenesis imperfecta, one of the dental genetic disease, is clinically and genetically complex disease. Amelogenesis imperfecta can be classified into three major categories according to clinical phenotype; hypoplastic, hypomaturation, and hypocalcification. Recently a novel gene, Fam83h, was identified to cause autosomal dominant hypocalcification amelogenesis imperfecta, however its functional role in the pathogenesis of enamel defect is not known yet. So this study was aimed to identify the knockdown effect of Fam83h gene on the amelogenin mRNA expression via shRNA transfection into immortalized ameloblast cell line. The result showed that the knockdown of Fam83h did not influence the amelogenin expression. Further study of the functional role of Fam83h gene should be performed to understand the complex nature of amelogenesis as well as molecular pathogenesis of amelogenesis imperfecta.

• Korean Journal of Clinical Laboratory Science
• /
• v.45 no.3
• /
• pp.102-107
• /
• 2013

Hepatitis A (HA) is an acute infectious disease of the liver caused by the Hepatitis A virus (HAV). In acute HA, the presence of anti-HAV IgM is detectable and about 3 weeks after exposure, its titre increases over 4 to 6 weeks. Anti-HAV IgG is detectable within a few days of the onset of symptoms. IgG antibodies continue to last for years after infection and provide lifelong immunity to the host. This study was performed to investigate the current seroprevalence of anti-HAV antibodies in Jeonbuk province, South Korea. A total of 591 (male 322, female 269) serum samples were collected in July 2011 to June 2012. We tested the antibodies of anti-HAV IgG and IgM using a Modular E170 (Roche Diagnostics, Germany), and analysed the serum alanine aminotransferase (ALT) levels by HITACH 7600-100 (HITACH, Japan). The overall seroprevalence of anti-HAV IgG was 84.6% (500/591), and the rate of females (85.9%) was higher than males (83.5%). According to the decade of age, seroprevalence of anti-HAV IgG were as follows; 68.8% (11/16) in the under 10 years old category, 100% (19/19) in the 10~19 category, 96% (48/50) in the 20~29 category, 83.6% (56/67) in the 30~39 category, 84.3% (123/146) in the 40~49 category, 83.3% (135/162) in the 50~59 category, 83.1% (54/65) in the 60~69 category, 78.1% (32/41) in the 70~79 category, and 88% (22/25) in the over 80 category. Total seroprevalence of anti-HAV IgM was 3.4% (20/591), and according to gender, the seroprevalence of male (3.1%) was very similar to that of female (3.7%). Through this study, we know that the seroprevalence of anti-HAV antibody in north-west Jeonbuk province, South Korea, was high. Only children under the age of 10 remain susceptible to HAV infection. Vaccination against HAV is not needed at the present time for the people of Jeonbuk province, South Korea, but a vaccination should be recommended and the improvement in sanitary conditions and personal hygiene should be highlighted.

• The Korea Journal of Herbology
• /
• v.35 no.5
• /
• pp.23-31
• /
• 2020

Objectives : This study was conducted to evaluate the anti-hyperlipidemia effect of Scutellariae Radix, Aucklandiae Radix and Bupleuri Radix(SAB). Methods : FL83B cells were mouse liver hepatocytes, and we used this cell line. FL83B cells were treated with 0.5 mM oleic acid(OA) for 24 h, SAB extract was treated. After OA treatment, intracellular triglyceride (TG) and free fatty acid contents were measured with AdiopoRed™ assay and Free Fatty Acid Quantitation assay kit, respectively. Further, we evaluated several lipogenesis and metabolic markers such as sterol regulatory element-binding transcription factor-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), 3-hydroxy3-methyl-glutaryl CoA reductase (HMGCR), hormone-sensitive lipase (HSL), carnitine palmitoyltransferase (CPT-1), peroxisome proliferator activated receptor alpha (PPARα), and cluster of differentiation (CD36) using RT-PCR and Western-blot analysis. Results : OA markedly increased intracellular TG and free fatty acid, which plays a key role in reducing hepatic lipid accumulation, in FL83B cells. These increases were alleviated by SAB extract. The mRNA and protein expression of Fatty acid(FA) oxidation factors (CPT-1, PPARα), lipolysis factor(HSL), FA transporter(CD36), cholesterol synthesis factors (HMGCoA) and Lipodenesis (SREBP-1c, FAS, and ACC-1) were significantly increased by treatment of SAB extract in the OA-induced fatty liver cell model. Conclusions : In summary, the treat of SAB extract showed a significant reduction of the influx of fatty acids into hepatocytes, promoted the oxidation of fatty acids, and regulated fat synthesis-related factors, thereby regulating the accumulation of TG and free fatty acids.

• Journal of The Korean Astronomical Society
• /
• v.49 no.3
• /
• pp.83-92
• /
• 2016

The Toothbrush radio relic associated with the merging cluster 1RXS J060303.3 is presumed to be produced by relativistic electrons accelerated at merger-driven shocks. Since the shock Mach number inferred from the observed radio spectral index, M_radio ≈ 2.8, is larger than that estimated from X-ray observations, M_X ≲ 1.5, we consider the re-acceleration model in which a weak shock of M_s ≈ 1.2 - 1.5 sweeps through the intracluster plasma with a preshock population of relativistic electrons. We find the models with a power-law momentum spectrum with the slope, s ≈ 4.6, and the cutoff Lorentz factor, γ_e,c ≈ 7-8×10⁴ can reproduce reasonably well the observed profiles of radio uxes and integrated radio spectrum of the head portion of the Toothbrush relic. This study confirms the strong connection between the ubiquitous presence of fossil relativistic plasma originated from AGNs and the shock-acceleration model of radio relics in the intracluster medium.

• Proceedings of the KSMRM Conference
• /
• 2002.11a
• /
• pp.83-83
• /
• 2002

목적： 현재 임상적으로 사용되고 있는 확산경사자장의 세기 이상( > 1200)의 강한 확산 경사자장이 가해진 경우 뇌구조물들의 확산계수값을 조사하고 고자장(Bo = 3T)에서 이러한 강한 확산 경사자장을 이용하는 경우 1.5T에서 시행하는 경우와의 차이점을 비교 연구해보고자 하였다. 대상 및 방법： 정상 성인 3명을 대상으로 b-value를 0부터 3500까지 변화시키며 확산강조영상을 최대경사자장 40 mT/m, slew rate 150 T/m/s의 경사자장계가 장착된 3T MR scanner (General Electric, USA)에서 획득하였다. 사용한 펄스열은 Stejskal-Tanner type의 확산강조 경사자장이 포함된 single-shot SE EPI를 사용하였으며 영상획득시 사용한 파라미터는 다음과 같다. TR/TE= 10000/95.1, Thickness/space=512mm, FOV=24$\times$21cm, Matrix=128$\times$128, NEX=1 뇌구조물들에 관심 영역을 설정하고 b-value에 따른 신호감소를 측정하였다. 측정된 데이터를 hi-exponential decay 모델을 이용하여 분석하였다.