Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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THE EFFECT OF Fam83h KNOCKDOWN ON THE AMELOGENIN GENE EXPRESSION IN THE AMELOBLAST CELL LINE

Fam83h 발현 억제에 의한 조법랑세포 Amelogenin 발현 변화

  • Lee, Sook-Kyung (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Lee, Kyung-Eun (Department of Pediatric Dentistry, School of Dentistry, Seoul National University) ;
  • Kim, Jung-Wook (Department of Pediatric Dentistry, School of Dentistry, Seoul National University)
  • 이숙경 (서울대학교 치과대학 소아치과학교실) ;
  • 이경은 (서울대학교 치과대학 소아치과학교실) ;
  • 김정욱 (서울대학교 치과대학 소아치과학교실)
  • Received : 2010.09.05
  • Accepted : 2010.11.04
  • Published : 2010.11.30
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Abstract

Amelogenesis imperfecta, one of the dental genetic disease, is clinically and genetically complex disease. Amelogenesis imperfecta can be classified into three major categories according to clinical phenotype; hypoplastic, hypomaturation, and hypocalcification. Recently a novel gene, Fam83h, was identified to cause autosomal dominant hypocalcification amelogenesis imperfecta, however its functional role in the pathogenesis of enamel defect is not known yet. So this study was aimed to identify the knockdown effect of Fam83h gene on the amelogenin mRNA expression via shRNA transfection into immortalized ameloblast cell line. The result showed that the knockdown of Fam83h did not influence the amelogenin expression. Further study of the functional role of Fam83h gene should be performed to understand the complex nature of amelogenesis as well as molecular pathogenesis of amelogenesis imperfecta.

치과유전질환의 하나인 법랑질 형성부전증은 유전적인 원인이 복잡할 뿐만 아니라 임상적인 양상 또한 다양하다. 법랑질 형성부전증은 임상적 양상에 따라서 크게 저형성형, 저성숙형, 저석회화형의 3 종류로 분류된다. 최근 상염색체 우성 저석회화 법랑질 형성부전증의 원인 유전자로 밝혀진 Fam83h의 기능에 관하여 알려진 바가 없어, Fam83h의 발현억제가 조법랑 세포의 아멜로제닌 발현에 미치는 영향을 불멸화된 조법랑세포주를 이용하여 분석한 결과, Fam83h의 발현이 억제되더라도 아멜로제닌의 발현에 영향을 주지 않는 것으로 확인되었으며, 향후 추가적인 연구를 통한 전체 유전자의 발현양상 변화 등을 통한 유전자 기능의 연구가 필요하리라 생각된다.

Keywords

References

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