• Title/Summary/Keyword: M49

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Detection of Babesia spp. in Free-Ranging Pukus, Kobus vardonii, on a Game Ranch in Zambia

  • Munang'andu, Hetron Mweemba;Munyeme, Musso;Nambota, Andrew Mubila;Nalubamba, King Shimumbo;Siamudaala, Victor M.
    • Parasites, Hosts and Diseases
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    • v.49 no.4
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    • pp.437-440
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    • 2011
  • Babesia spp. were detected from 4 asymptomatic pukus captured on a game ranch in central Zambia in October 2008. Blood smears were examined in 4 species of aymptomatic free-ranging antelopes, namely the puku (Kobus vordanii), reedbuck (Redunca arundinum), bushbuck (Tragelaphus sylvaticus), and kudu (Tragelaphus strepsiceros), and showed the presence of Babesia parasites only in the puku. In the puku, the prevalence of babesiosis was estimated at 33.3% (n=12), while the overall prevalence in all examined animals was 8.5% (n=47). The parasites showed morphological characteristics of paired ring-like stages with the length varying between $1.61{\mu}m$ and $3.02{\mu}m$ ($mean=2.12{\mu}m$, n=27; $SD=0.76{\mu}m$). Both the infected and non-infected pukus showed good body condition scores (BCS), while the dominant tick species detected from all animals were Rhipicephalus appendiculatus, Rhipicephalus spp., and Boophilus spp. To our knowledge this is the first report of Babesia spp. infection in pukus in Zambia. These findings suggest that wildlife could play an important role in the epidemiology of babesiosis in Zambia.

Radiographic manifestations of fibroblastic osteosarcoma: A diagnostic challenge

  • Tahmasbi-Arashlow, Mehrnaz;Barnts, Kelcie Louise;Nair, Madhu K.;Cheng, Yi-Shing Lisa;Reddy, Likith V.
    • Imaging Science in Dentistry
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    • v.49 no.3
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    • pp.235-240
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    • 2019
  • Osteosarcoma is the most common primary bone tumor after plasma cell neoplasms. Osteosarcoma has diverse histological features and is characterized by the presence of malignant spindle cells and pluripotent neoplastic mesenchymal cells that produce immature bone, cartilage, and fibrous tissue. Osteosarcoma most frequently develops in the extremities of long bones, but can occur in the jaw in rare cases. The clinical and biological behavior of osteosarcoma of the jaw slightly differs from that of long-bone osteosarcoma. The incidence of jaw osteosarcoma is greater in the third to fourth decades of life, whereas long-bone osteosarcoma mostly occurs in the second decade of life. Osteosarcoma of the jaw has a lower tendency to metastasize and a better prognosis than long-bone osteosarcoma. Radiographically, osteosarcoma can present as a poorly-defined lytic, sclerotic, or mixed-density lesion with periosteal bone reaction response. Multi-detector computed tomography is useful for identifying the extent of bone destruction, as well as soft tissue involvement of the lesion. The current case report presents a fibroblastic osteosarcoma involving the left hemimandible with very unusual radiographic features.

Treatment Study of Textile Wastewater by Fenton's Oxidation (펜톤 산화반응에 의한 염색폐수처리 연구)

  • 박영규
    • Journal of environmental and Sanitary engineering
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    • v.15 no.4
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    • pp.20-25
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    • 2000
  • The wastewater treatment for the purpose of water-recycling was performed using Fenton's & ozone's methods. These methods were used to increase the treatment efficiency of textile wastewater and to search for the optimal operating conditions. The optimal conditions by Fenton process were determined so that input amounts of $FeSO_4{\cdot}7H_2O$ and $H_2O_2$ were $7.2mM/{\;}{\ell}$ and $49.0m/{\;}{\ell}$ respectively, treatment by ozone process had 92% removal efficiency at ozone concentration of 9.73g/min and $130mM/{\;}{\ell}$ of $H_2O_2$.

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Effect of Mercuric Chloride (In Vitro) on the Function of Human Polylnorphonuclear Leukocytes(PMNs) (수은이 시험관내 사람 다형핵백혈구의 기능에 미치는 영향)

  • 한형미;윤은이;김순한;김옥연;김효정;선우연
    • Biomolecules & Therapeutics
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    • v.1 no.2
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    • pp.131-136
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    • 1993
  • In the present study, the effect of $HgCl_2$on the function of human peripheral polymorphonuclear leukocytes(PMNs) was examined. PMNs were isolated from human peripheral blood with density centrifugation in Ficoll-Paque. The cells were then incubated with $0.5{\sim}5{\mu}M\;HgCl_2$and glass adherence, chemotactic activity and erythrocyte-antibody rosette forming activity were measured. $HgCl_2$ decreased the function of PMNs in all three aspects tested. $HgCl_2$significantly diminished glass adherence(40.5 {\mu}M: 92{\pm}12%$ (percentage of control, $mean{\pm}$ S.D.); 41 {\mu}M: 46{\pm}11%,$ P<0.01; $3{\mu}M: 35{\pm}7%,$P<0.01;$5{\mu}M:49{\pm}10%,$ P<0.01). Similarly, significant differences were observed in chemotactic activity after $HgCl_2$treatment compared with control (control: $0.95{\pm}0.14mm; 0.5 {\mu}M: 0.91{\pm}0.11 mm; 1 {\mu}M: 0.77{\pm}0.16mm, P<0.05; 3{\mu}M: 0.61{\pm} 0.06mm, P<0.01; 5{\mu}M: 0.15{\pm}0.03 mm, P<0.01).$ Also, 4HgCl_2$decreased the percentage of rosette-forming PMNs, indicating diminished phagocytic activity of PMNs upon $HgCl_2$ exposure compared with control (control: $58{\pm}4%; 1{\mu}M: 53{\pm}4%, p<0.05; 3{\mu}M: 49{\pm}3%, P<0.01; 5{\mu}M: 46{\pm}3%, P<0.01).$ Cell viability was not antered after $HgCl_2$treatment (483{\pm}5%$ viability in control PMNs versus $81{\pm}8%$ viability in $5{\mu}M$ Hg-treated PMNs), suggesting that the impaired PMN function after $HgCl_2$treatment was not due to nonspecific cytotoxicity induced by $HgCl_2$. $HgCl_2$-induced decrease in the function of PMNs may have some implications in depressed host susceptibilityupon bacterial challenge after mercury exposure.

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A Weak Signal Detection Algorithm in Clutter Environment for Indoor Location Estimation based on IR-UWB Radar (IR-UWB 레이더 기반의 실내 위치 추정을 위한 클러터 환경에서 미약신호 검출 알고리즘)

  • Younguk Yun;Jung-woo Sohn;Youngok Kim
    • Journal of the Society of Disaster Information
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    • v.19 no.1
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    • pp.10-17
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    • 2023
  • Purpose: In this paper, a distance estimation technique for indoor location estimation using IR-UWB is proposed and researched. We propose an algorithm that can increase the SNR lowered due to clutter or noise in an indoor environment. Method: In order to clutter suppression and detect weak signals, we analyze the existing studies of background remover, correlation, and singular vector decomposition techniques and propose an algorithm. Result: The proposed algorithm, the average error was 0.57m up to 11.5m, and the error were 0.49m from 6m to 11.5m. the average error rate was reduced by about 1m compared to the previous study. Conclusion: It can be used as a technique for detecting weak signals in clutter and noise environments for distance or location estimation, and can also be used as a human life detection technique to reduce damage to people in a disaster situation by using UWB radar which has highly transparent.

Simple and Rapid Extraction of a Bacteriocin Produced by Streptococcus parauberis Z49 from Fermented Cultures (발효배양액에서 Streptococcus parauberis Z49균주가 생산하는 Bacteriocin의 간편한 추출)

  • Park, Hong-Je;Khang, Yong-Ho
    • Korean Journal of Microbiology
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    • v.46 no.3
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    • pp.291-295
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    • 2010
  • A novel bacteriocin produced by Streptococcus parauberis Z49 strain was characterized and efficiently extracted from fermented cultures by use of aqueous two-phase systems. The nisin-like bacteriocin, which was active even after a heat treatment at $121^{\circ}C$ for 15 min and in the broad pH range from 2 to 12, showed inhibition of bacterial growth of Micrococcus luteus, Lactobacillus spp., Lactobacillus fermentum, Enterococcus faecium, Listereia monocytogenes, and Pseudomonas fluorescens. Optimal conditions of PEG 600/$Na_2SO_4$ aqueous two-phase systems for the simple and rapid extraction of a novel bacteriocin were determined to be PEG 600 15%, $Na_2SO_4$ 30%, and NaCl 8%, where the bacteriocin was concentrated in PEG layer.

Short -term changes of microbial communities after control of Cochlodinium polykrikoides by yellow clay and chemical compound dosing in microcosm experiments (황토와 화학물질 살포에 의한 적조생물Cochlodinium polykrikoides 제어에 따른 미소생물그룹의 단주기변화)

  • Baek, Seung Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.4
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    • pp.2971-2977
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    • 2015
  • This study aimed to understand the changes in microbial community after algicide dosing to control the fish-killing dinoflagellate Cochlodinium polykrikoides in 10L microcosm. Based on our microcosm experiments, the algicidal activity for C. polykrikoides of yellow clay at the concentrations of 4g and 10g per 10 L was < 20%. At $0.8{\mu}M$ concentration of thiazolidinedione(TD49), the population of C. polykrikoides was controlled to be > 85%. In microbial community, a significant increase in heterotrophic bacterial (HB) abundance was observed at day 1 in the TD49 and yellow clay treatments including control. The HB remained high for 2 days and then gradually decreased. In contrast, the abundance of heterotrophic nanoflagellates (HNFs) increased significantly on days 3 and 5 in the TD49 treatments, indicating that the decline in HB was probably a result of predation by the high density of HNFs. In addition, fluctuations in the aloricate ciliate Uronema sp., which feed on bacteria, was clearly correlated with fluctuations in HB abundance, with a lag period of 1-3 days. Therefore, the short-term responses of the HNF and Uronema sp. may have been a result of the rapidly increasing of HB abundance, which is related to degradation of the dense C. polykrikoides bloom, particularly in the TD49 treatment. In addition, large aloricate ciliate Euplotes sp. was significantly increased after reproduction of HNFs and Uronema sp. Consequently, the algicide TD49 had positive effect on the microbial communities, which indicates that the microbial loop was temporarily enhanced in the microcosm by energy flow from HB through HNFs to ciliate.