• Proceedings of the Korean Geotechical Society Conference
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• 2002.10a
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• pp.213-227
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• 2002

절토사면의 붕괴원인은 토질 및 지질조건, 지형, 강우, 지하수 및 지표수, 사면형상, 굴착 및 발파와 같은 인위적인 조건, 사면보호공 등과 같이 다양한 영향이 있을 수 있으나 가장 많은 영향을 주는 원인으로 토질 및 지질적인 조건이라고 할 수 있다. 본 논문은 이러한 지질조건에서 붕괴가 발생될 경우의 활동면에 대한 전단강도 추정하는 방법에 있어 역해석법에 의한 활동면의 전단강도 추정을 연구하였다 연구결과 붕괴된 사면에서 화성암은 마찰각 20$^{\circ}$~30$^{\circ}$, 점착력 0~2t/$m^2$의 범위를 가지며 퇴적암에서는 마찰각 $10^{\circ}$~17$^{\circ}$, 점착력 0~2.5t/$m^2$의 범위, 변성암에서는 마찰각 $10^{\circ}$~40$^{\circ}$, 점착력 0~4.0t/$m^2$의 범위가 우세한 것으로 나타났다. 그리고 지질구별 구분에 의하면, 절리에 의해 붕괴가 발생된 경우에는 마찰각 30$^{\circ}$~40$^{\circ}$, 점착력 0~3.5t/$m^2$, 엽리면은 마찰각 30$^{\circ}$~35$^{\circ}$, 점착력 0.5~3.0t/$m^2$, 단층면은 마찰각 11$^{\circ}$~38$^{\circ}$, 점착력 0~3.0t/$m^2$, 층리면은 마찰각 $10^{\circ}$~17$^{\circ}$, 점착력 0~2.5t/$m^2$ 정도의 범위를 갖는 것으로 나타났다.

• Korean Journal of Microbiology
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• v.27 no.4
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• pp.422-429
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• 1989

Auxotrophic mutant were isolated from wild types by the treatment with NTG as a mutagen, and the conditions of protoplast formation for them were established. The protoplasts of killer yeast Saccharomyces cerevisiae K52 were formed to the level of above 70% when cells grown for 20 hr in PM medium were treated with 200 unit/ml Lyticase 50,000 at $30^{\circ}C$ for 60 min after pretreatment of 50 mM 2-mercaptoethanol in 10mM potassium phosphate buffer (pH 7.5) containing EDTA and 0.6 M sorbitol for 15 min. Also, the protoplast of the recipient S. cerevisiae S 29 were formed to the level of above 85% as it was cultured to the log phase of 24 hr in PM medium under the same conditions. The fusion frequency between the protoplast of killer yeast S. cerevisiae K 52 and the protoplast of recipient S. cerevisiae S 29 was reached to $8.2\times 10^{-6}$ when the hypertonic regeneration medium embeded with the fused protoplasts after mixing the parental protoplasts to 10$^{8}$ cells/ml in SP buffer containing 20 mM $CaCl_{2}$ and 30% PEG 6,000 for 15 min at $30^{\circ}C$ were incubated.

• Korean Journal of Environmental Agriculture
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• v.21 no.2
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• pp.102-108
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• 2002

In order to investigate the mechanism of growth inhibition by salt stress, lettuces were grown hydroponically in three different nutrient solutions, normal and 30 mM or 50 mM $KNO_3$-added nutrient solutions, and the electrical conductivities of these solutions were 1.0, 4.5, and 6.5 dS/m, respectively. The activities of plasma and vacuolar $H^+$-ATPases in the root tissue of lettuce were measured by specific inhibitors, 100 ${\mu}M$ vanadate and 50 mM $NO_3^-$, respectively. Microsomal ATPase activity of lettuce grown in the normal nutrient solution was $356\pm1.5$ nmol/min/mg protein. When lettuces were grown in 30 mM and 50 mM $KNO_3$-added nutrient solutions, total activities of microsomal ATPases were increased by 1.6 and 1.9 times, respectively, and the increases were mainly mediated by vacuolar $H^+$-ATPase. These results show that lettuces adapt themselves to salt-stressed condition by increasing the activities of $H^+$-ATPases. Effects of various heavy metal ions were investigated on the microsomal ATPases and various metal ions at 100 $\mu M$ inhibited the activities by 10$\sim$25%. $Cu^{2+}$ showed the highest inhibitory effect on the vacuolar $H^+$-ATPase. These results suggest that lettuce increases the activities of root ATPases, specially that of vacuolar $H^+$-ATPase, in salt-stressed growth conditions and $Cu^{2+}$ could be a useful tool to control the activity of vacuolar $H^+$-ATPase.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.50.2-50.2
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• 2015

전파천문학의 불모지였던 우리나라에 전파천문학이 잉태된 것은 1980년으로 국립천문대가 발족한지 불과 6년이 지난 시점으로, 소백산 61cm 광학망원경의 광전측광관측이 궤도에 진입하던 시기였다. 우리나라에서 현대천문학이 겨우 걸음마를 할 시기에 mm파 전파망원경의 도입은 용감한 도전으로, 5년여 간 많은 우여곡절을 겪으며 1985년에 전파망원경이 설치되었다. 1985년 대덕에 14m 전파망원경을 건설하며 시작된 우리나라의 전파천문학은 지난 30년간 발전을 거듭하며 전파간섭계인 한국우주전파관측망(KVN) 건설과 태양전파망원경 설치로 이어져 AGN, 원시성 및 만기형성의 미세구조 연구, 태양폭발현상 연구 등 연구 영역을 확장하고 있다. 2015년은 14m 전파망원경 건설 30주년을 맞는 해이다. 본 발표에서는 대덕전파천문대가 지난 30년간 걸어온 발자취를 살펴보고, 이를 기반으로 한 발전방향을 논의하고자 한다.

• Journal of Environmental Science International
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• v.5 no.4
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• pp.441-451
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• 1996

The surface meteorological and upper layer meteorological observation carried out to investigate influences of sea breeze effect on lower layer atmosphere at Gori nuclear power plant for 29∼30 July, 1996. According to surface meteorological data, the inflow of sea breeze was occurred 11:30 on 29 July, 10:30-on 30 July, respectively, at observation site. And the meteorological tower data showed that wind direction of sea breeze was identified as south-westerly, and wind speed of 58 m was 2 times stronger than that of 10 m. It is notworthy that surface inversion layer which built from the night time to daybreak of next day was not broken off by seab reeze's inflow for daytime, and strong inversion layer observed at 47∼243 m with moderately stable class (F) by URC. It was found that strong stable layer of potential temperature appeared at that layer, maximum relative humidity observed at the bottom of inversion layer and maximum mixing ratio observed in the low of inversion layer.

• Environmental Analysis Health and Toxicology
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• v.18 no.3
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• pp.231-235
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• 2003

To determine whether or not bisphenol A affects the Immune system, female ICR mice were treated bisphenol A (BPA) orally at the doses of 100, 500 and 1,000 mg/kg for 30 consecutive days. Four days before enumerating Plaque -forming cells (PFCs) mice were immunized intraperitoneally with sheep red blood cells (SRBCs). The spleen cellularity and PFC/spleen were significantly reduced by 30-day exposure to BPA (1,000 mg/kg/day), but the PFC/10$\^$6/ spleen cells was slightly decreased.. When splenocytes isolated from the mice exposed to BPA for 30 days were cultured in the presence of LPS, Con A or PHA with IL-2, the lymphocyte proliferation ex vivo was not significantly suppressed by BPA. Our present results indicated that 30-day exposure of mice to BPA might have mild immunotoxic potential.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.983-988
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• 2007

The aim of this study was to examine the correlation between bovine casein (CN) mRNA expression levels in mammary epithelial cells and lactation period, the yields of milk proteins and other parameters. The cells were collected from each cow's milk, which contained somatic cell counts (SCC) of less than 100,000 cells/ml. The levels of ${\alpha}s1-$, ${\alpha}s2-$, ${\beta}$- and ${\kappa}$-CN mRNA expression were significantly correlated with each other in mammary epithelial cells (p<0.01). All cows produced either less than 30 kg/day/cow or a over 30 kg/day/cow level of milk yield (MY). It was shown that the CN mRNA expression levels decreased gradually from the calving period to late lactation, when MY was over 30 kg/day/cow. The SCC tended to increase gradually during the course of lactation, but it was negatively correlated with milk protein and CN yields (p<0.01) when MY was less than 30 kg/day/cow. Moreover, there was a tendency for a negative correlation between SCC and ${\alpha}s1$-CN and ${\beta}$-CN mRNA expression level, when MY was less than 30 kg/day/cow (p<0.05).

• Journal of Korean Ophthalmic Optics Society
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• v.5 no.1
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• pp.83-87
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• 2000

To investigate exhausted-medium-induced apoptosis in human corneal epithelial(HCE) cells, this study was performed DNA gel electrophoresis, M30 CytoDEATH staining and FAS-FAS ligand ELISA. SV-40 transfected cells were grown to confluency in culture for 7days. The supernatant was harvested and filtered with $0.22{\mu}m$ filter paper. Fresh HCE cells were exposed to the filtered exhausted medium for 1~2 days. Apoptotic cells were prepared for DNA extraction and run the agarose gel for DNA ladder pattern. M30 CytoDEATH was used a tool for easy and reliable determination of very early apoptosis in HCE cells. The control and exhausted medium were assayed for soluble FAS/FAS ligand protein by ELISA. HCE cells exposed to exhausted medium showed a typical DNA ladder pattern. Sporadic M30 CytoDEATH positive cells were detected among HCE cells exposed to exhausted medium. Soluble FAS/FAS ligand levels were not elevated in the exhausted medium compared to the fresh medium control. This study suggests that possible mechanism of exhausted medium induced apoptosis does not include the FAS-FAS ligand system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.3
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• pp.2029-2037
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• 2015

The purpose of this study was to investigate the effect the interferential current (IFC) stimulation level according to autonomic nerve system (ANS) of healthy adults. IFC measured with adhesive 2-pole electrodes pad on T1~T4 spinal cord segment level that electrode stimulation of 20 min after. A total of 45 healthy adults participated in the study, who were randomly divided into three groups: 15 of sensory level stimulus group (100 bps, 10~12 mA), 15 of exercise level stimulus group (5 bps, 45~50 mA), 15 of noxious level stimulus group (100 bps, 80~90 mA). Measured items were sympathetic activity and parasympathetic activity. In order to identify the changes in measurement variables before electrode stimulation and after and 30 min stimulation. The comparison of sympathetic and parasympathetic activity in each group revealed that they was changes of after vs 30 min in after electrical stimulation time(p<.05).

• Environmental Engineering Research
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• v.25 no.1
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• pp.96-103
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• 2020

The aim of this study is to investigate the effect of five different combinations including: sand 70%, bentonite 30% (S₇₀B₃₀)- sand 80%, bentonite 20% (S₈₀B₂₀)- sand 80%, organophilic 20% (S₈₀M₂₀)- sand 60%, bentonite 20%, organophilic 20% (S₆₀B₃₀M₂₀) and sand 75% - bentonite 15% - organophilic 10% (S₇₅B₁₅M₁₀) on landfill linear structure in order to decrease phenol leaching. Hydraulic conductivity and adsorption behavior of the samples were investigated. The results demonstrated that the lowest hydraulic conductivity coefficient ($1.16{\times}10^{-11}{\frac{m}{s}}$) was obtained for S₇₀B₃₀. Furthermore, adding more than 20% of bentonite had no significant effect on reducing permeability. Moreover, Freundlich isotherm was introduced as the best model explaining adsorption behaviour due to its highest determination coefficient (0.945). The best samples for adsorption capacity of phenol and for both permeability and adsorption are S₈₀M₂₀ and S₆₀B₃₀M₂₀, respectively. Although the presence of bentonite was effective in reducing hydraulic conductivity, organic clay had no considerable impact on reducing permeability. Though, it's an exceptional role in adsorbing organic contaminants including phenol cannot be ignored. To meet all regulatory constraints, the optimal compound is made up of 10.2% of bentonite and 2.8% of organophilic clays with a minimized cost of 13.64 ($/ton).