• Analytical Science and Technology
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• v.19 no.5
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• pp.407-414
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• 2006

An X-ray diffractometer for spatially resolved X-ray diffraction measurements was developed to identify phase in the narrow (micron-scaled) region of high burn-up fuels and some nuclear materials. The micro-XRD was composed of an X-ray microbeam alignment system and a sample micro translation system instead of a normal slit and a fixed sample stage in a commercial XRD. The X-ray microbeam alignment system was fabricated with a microbeam concentrator having two Ni deposited mirrors, a vertical positioner, and a tilt table for the generation of a concentrated microbeam. The sample micro translation system was made with a sample holder and a horizontal translator, allowing movement of a specimen at $5{\mu}m$ steps. The angular intensity profile of the microbeam generated through a concentrator was symmetric and not distorted. The size of the microbeam was $4,000{\times}20{\mu}m$ and the spatial resolution of the beam was $47{\mu}m$ at the sample position. When the diffraction peaks were measured for a $UO_2$ pellet specimen by this system, the reproducibility ($2{\Theta}={\pm}0.01^{\circ}$) of the peaks was as good as a conventional X-ray diffractometer. For the cross section of oxidized titanium metal, not only $TiO_2$ in an outer layer but also TiO near an oxide-metal interface was observed.

• Animal cells and systems
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• v.14 no.3
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• pp.147-154
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• 2010

It is well-established that phosphoinositide 3-kinase (PI3-kinase) regulates myogenesis by inducing transcription of myogenin, a key muscle regulatory factor, at the initiation of myoblast differentiation. In this study, we investigated the role of PI3-kinase in cells that have committed to differentiation. PI3-kinase activity increases during myogenesis, and this increase is sustained during the myogenic process; however, its function after the induction of differentiation has not been investigated. We show that LY294002, a PI3-kinase inhibitor, blocked myoblast fusion even after myogenin expression initially increased. In contrast to the inhibitory effects of LY294002 on myogenin mRNA levels during the initiation of differentiation, LY294002 blocked the accumulation of myogenin protein without affecting its mRNA level after differentiation was induced. Treatment with cycloheximide, a translation inhibitor, or actinomycin D, a transcription inhibitor, indicated that the stability of myogenin protein is lower than that of its mRNA. LY294002 inhibited the activities of several important translation factors, including eukaryotic elongation factor-2(eEF2), by altering their phosphorylation status. In addition, LY294002 blocked the incorporation of [$^{35}S$]methionine into newly synthesized proteins. Since myogenin has a relatively short half-life, LY294002-mediated inhibition of post-transcriptional processes resulted in a rapid depletion of myogenin protein. In summary, these results suggest that PI3-kinase plays an important role in regulating the expression of myogenin through post-transcriptional mechanisms after differentiation has been induced.

• Journal of Microbiology
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• v.44 no.2
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• pp.200-205
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• 2006

The tributyltin (TBT)-resistant bacterium, Pseudomonas aeruginosa 25W, which was isolated in seawater from the Arabian Sea, was subjected to transcriptome analysis in the presence of high concentrations of TBT. Only slight effects were observed at TBT concentration of $50{\mu}M$, but exposure to $500{\mu}M$ resulted in the upregulation of 6 genes and the downregulation of 75. Among the 75 downregulated genes, 53% (40 out of 75) were of hypothetical function, followed by 14 transcriptional regulation- and translation-associated genes. The results of this study indicated that although the 25W strain was highly resistant to TBT, high concentrations of TBT result in toxic effect on the transcriptional and translational levels. The target genes likely belong to a specific category of transcription- and translation-associated genes rather than to other gene categories.

• Communications of the Korean Mathematical Society
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• v.19 no.1
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• pp.119-127
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• 2004

Suppose that $\psi{\;}\in{\;}L^2(\mathbb{R})$ generates a wavelet frame (resp. Riesz basis) with bounds A and B. If $\phi{\;}\in{\;}L^2(\mathbb{R})$ satisfies $$\mid$\^{\psi}(\xi)\;\^{\phi}(\xi)$\mid${\;}<{\;}{\lambda}\frac{$\mid$\xi$\mid$^{\alpha}}{(1+$\mid$\xi$\mid$)^{\gamma}}$ for some positive constants $\alpha,{\;}\gamma,{\;}\lambda$ such that $1{\;}<1{\;}+{\;}\alpha{\;}<{\;}\gamma{\;}and{\;}{\lambda}^2M{\;}<{\;}A$, then $\phi$ also generates a wavelet frame (resp. Riesz basis) with bounds $A(1{\;}-{\;}{\lambda}\sqrt{M/A})^2{\;}and{\;}B(1{\;}+{\;}{\lambda}\sqrt{M/A})^2$, where M is a constant depending only on $\alpha,{\;}\gamma$ the dilation step a, and the translation step b.

• Journal of Sasang Constitutional Medicine
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• v.19 no.3
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• pp.99-125
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• 2007

1. Objectives This study focuses on the Revision and Translation of the Mongolian's Sasang constitution Diagnosis Questionnaire (SDQ-M) 2. Methods 03'SDQ's questionnaire analysis study have been performed based on the absolutely diagnosed group of 423 cases who have respond to the Sasang constitution Diagnosis Questionnaire (SDQ) from June to November, 2003. 3. Results and Conclusions Revised 05'SDQ-M's traits, which result from on the basis of 03'SDQ's questionnaire analysis study results and socio-cultural understanding via Mongolian physical constitution diagnosis as well as Mongolian translators' interchange, are as follows. (1) 05'SDQ-M is mainly adjusted in terms of external appearances and the nature of illness symptoms. (2) 05'SDQ-M's physical constitution questionnaires are equally adjusted. (3) 05'SDQ-M is re-used, deleted, or created on the basis of 03'SDQ's questionnaire analysis results. (4) 05'SDQ-M is translated to be easy for Mongolians.

• Korean Journal of Applied Biomechanics
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• v.31 no.3
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• pp.176-182
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• 2021

Objective: The purpose of this study is to investigate whether the athletic knee show greater rotation and translation movement than non-athletic knee during the treadmill walking with their preferred speed in a complete gait cycle. Method: Thirty young and healthy male subjects participated in the study, fifteen handball players (mean age: 19.6 ± 1.4 years old, mean weight: 85 ± 11.9 Kg, mean height: 179.8 ± 4.7) and fifteen non-athletes (mean age: 22.8 ± 1.2 years old, mean weight: 74.5 ± 8.6 Kg, mean height: 175 ± 5.9). Three-dimensional positional coordinate of lower limb during treadmill walking were analyzed. Results: There were significant differences (t (22.014)=1.585, p=0.127 in the range of internal and external rotation with mean value for handball player (M=14.4513, SD=2.3839) was higher than non-athletes (M=13.3327, SD=1.337). The magnitude of the difference in the means (mean difference=1.11867, 95% CI: -0.34489 to 2.5822) was significant. There were also significant differences (t (17.956)=1.654, p=0.116 in the max abduction and adduction with mean value for handball player (M=5.7160, SD=2.49281) was higher than non-athletes (M=4.5773, SD=0.94667). The magnitude of the difference in the means (mean difference=1.138, 95% CI: -0.30805 to 2.58539) was significant. At significance level 0.05. Conclusion: Finding of this study suggest that to understand the actual characteristic of knee motion studies have to be done in different walking and running trial at variable speed.

• Journal of Internet Computing and Services
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• v.5 no.6
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• pp.45-58
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• 2004

This paper consists of two parts, One is the ECC( GF(2/sup m/)) algorithm to improve the security strength and the processing time of SSL VPN and the other is the WLCAPT algorithm instead of LSNAT for the security strength of virtual server. In general when corporates use SSL protocol in order to build VPN, they use RSA algorithm with the problem of security and processing time about authentication and confidentiality, In this paper, a shared public key is generated with ECSPK which uses ECC( GF(2/sup m/)) algorithm to improve the security and processing time instead of RSA In addition, WLCAPT algorithm proposed in this paper is applied to virtual server which resides in the server side and then after NAT translation, the actual server of headquarter is securely communicated with it.

• Journal of Integrative Natural Science
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• v.12 no.4
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• pp.127-132
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• 2019

Programmed cell death 4 (PDCD4) is a novel tumor suppressor that function in the nucleus and the cytoplasm and appears to be involved in the regulation of transcription and translation. Stress granules (SGs) are cytoplasmic foci at which untranslated mRNAs accumulate when cells exposed to environmental stresses. Since PDCD4 has implicated in translation repression through direct interaction with eukaryotic translation initiation factor 4A (eIF4A), we here investigated if PDCD4 has a functional role in the process of SG assembly under oxidative stresses. Using immunofluorescence microscopy, we found that PDCD4 is localized to SGs under oxidative stresses. Next, we tested if knockdown of PDCD4 has an effect on the assembly of SG using PDCD4-specific siRNA. Interestingly, SG assembly was accelerated and this effect was caused by sensitization of phosphorylation of eIF2α and dephosphorylation of eIF4E binding protein (4E-BP). These results suggest that PDCD4 has an effect on SG dynamics and possibly involved in cap-dependent translation repression under stress conditions.

• Applied Biological Chemistry
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• v.40 no.5
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• pp.375-379
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• 1997

Two ribosome-inactivating proteins, PRIP 1 and PRIP 2 have been isolated from the leaves of $Cucurbita\;moschata\;D_{UCHESNE}$. Crude extracts were purified through ammonium sulfate precipitation and column chromatography using DE-52 cellulose, S-Sepharose, FPLC Suprose 12 HR and FPLC Mono-S. The molecular weights of PRIP 1 and PRIP 2 were 31,000 and 30,500, respectively. PRIP 2 was thermostabe and maintained its activity even after the incubation of the protein at $50^{\circ}C$ for 30 min. In a cell free in vitro translation system using rabbit reticulocyte lysate, protein synthesis was inhibited by the addition of PRIP 1 and PRIP 2. The $IC_{50}$ of PRIP 1 and PRIP 2 were 0.82 nM and 0.79 nM, respectively. The comparison of N-terminal amino acid sequences of the PRIP 1 and PRIP 2 with known RIPs revealed that PRIP 1 shows sequence similarity with Luffin B from Luffa cylindrica and Trichokirin from Trichosanthes kirilowii Maximowicz and PRH) 2 has sequence similarity with Momordin II and MAP 30 from Momordica charantia.

• BMB Reports
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• v.47 no.10
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• pp.558-562
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• 2014

OASL1 is a member of the 2'-5'-oligoadenylate synthetase (OAS) family and promotes viral clearance by activating RNase L. OASL1 interacts with the 5'-untranslated region (UTR) of interferon regulatory factor 7 (Irf7) and inhibits its translation. To identify the secondary structure required for OASL1 binding, we examined the 5'-UTR of the Irf7 transcript using "selective 2'-hydroxyl acylation analyzed by primer extension" (SHAPE). SHAPE takes advantage of the selective acylation of residues in single-stranded regions by 1-methyl-7-nitroisatoic anhydride (1M7). We found five major acylation sites located in, or next to, predicted single-stranded regions of the Irf7 5'-UTR. These results demonstrate the involvement of the stem structure of the Irf7 5'-UTR in the regulation of Irf7 translation, mediated by OASL1.