• Molecules and Cells
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• v.39 no.12
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• pp.841-846
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• 2016

Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.

• Journal of Magnetics
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• v.18 no.3
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• pp.308-310
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• 2013

Translation induced by the field-gradient force is being observed for a single ferromagnetic iron grain and a ferrimagnetic grain of a ferrite sample ($CuFe_2O_4$). From measurements on the translation, precise saturated magnetization of $M_S$ is possible for a single grain. The method is based on the energy conservation rule assumed for the grain during its translation and the grain is translated through a diffuse area under microgravity conditions. The results of the two materials indicate that a field-induced translation of grain bearing spontaneous moment is generally determined by a field-induced potential $-mM_SH(x)$ where m denotes the mass of sample. According to the above translations, the detection of $M_S$ is not interfered by any signals from the sample holder. The $M_S$ measurement does not require m value. By observing translations resulting from fieldinduced volume forces, the magnetization of a single grain is measurable irrespective of its size; the principle is also applicable to measuring susceptibility of diamagnetic and paramagnetic materials.

• BMB Reports
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• v.43 no.5
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• pp.344-348
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• 2010

Messenger ribonucleoprotein particles (mRNPs) are used to transport mRNAs along neuronal dendrites to their site of translation. Staufen2 is an mRNA-binding protein expressed in the cell bodies and cellular processes of different brain cells. It is notably involved in the transport of dendritic mRNAs along microtubules. Its knockdown expression was shown to change spine morphology and impair synaptic functions. However, the identity of Staufen2-bound mRNAs in brain cells is still completely unknown. As a mean to identify these mRNAs, we immunoprecipitated Staufen2-containing mRNPs from embryonic rat brains and used a genome wide approach to identify Staufen2-associated mRNAs. The genome wide approach identified 1780 mRNAs in Staufen2-containing mRNPs that code for proteins involved in cellular processes such as post-translational protein modifications, RNA metabolism, intracellular transport and translation. These results represent an additional and important step in the characterization of Staufen2- mediated neuronal functions in rat brains.

• ETRI Journal
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• v.30 no.2
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• pp.326-334
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• 2008

This paper proposes a method for generating a basis translation matrix between isomorphic extension fields. To generate a basis translation matrix, we need the equality correspondence of a basis between the isomorphic extension fields. Consider an extension field $F_{p^m}$ where p is characteristic. As a brute force method, when $p^m$ is small, we can check the equality correspondence by using the minimal polynomial of a basis element; however, when $p^m$ is large, it becomes too difficult. The proposed methods are based on the fact that Type I and Type II optimal normal bases (ONBs) can be easily identified in each isomorphic extension field. The proposed methods efficiently use Type I and Type II ONBs and can generate a pair of basis translation matrices within 15 ms on Pentium 4 (3.6 GHz) when $mlog_2p$ = 160.

• Journal of Microbiology and Biotechnology
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• v.16 no.3
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• pp.355-359
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• 2006

The functional stability of mRNA is one of the crucial factors affecting the efficiency of in vitro translation. As the rapid degradation of mRNA in the cell extract (S30 extract) causes early termination of the translational reactions, extending the mRNA half-life will improve the productivity of the in vitro protein synthesis. Thus, a simple PCR-based method is introduced to increase the stability of mRNA in an S30 extract. The target genes are PCR-amplified with primers designed to make the ends of the transcribed mRNA molecule anneal to each other. When compared with normal mRNA, the mRNA with the annealing sequences resulted in an approximately 2-fold increase of protein synthesis in an in vitro translation reaction. In addition, sequential transcription and translation reactions in a single tube enabled direct protein expression from the PCR-amplified genes without any separate purification of the mRNA.

• BMB Reports
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• v.33 no.2
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• pp.103-111
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• 2000

Phosphorylation of the eukaryotic elongation factor 2 (eEF-2) blocks the elongation step of translation and stops overall protein synthesis. Although the overall rate of protein synthesis in mitosis reduces to 20% of that in S phase, it is unclear how the protein translation procedure is regulated during the cell cycle, especially in the stage of peptide elongation. To delineate the regulation of the elongation step through eEF-2 function, the changes in phosphorylation of eEF-2, and in activity of corresponding $Ca^{2+}$/calmodulin (CaM)-dependent protein kinase III (CaMK-III) during the cell cycle of NIH 3T3 cells, were determined. The in vivo level of phosphorylated eEF-2 showed an 80% and 40% increase in the cells arrested at G1 and M, respectively. The activity of CaMK-III also changed in a similar pattern, more than a 2-fold increase when arrested at G1 and M. The activity change of the kinase during one turn of the cell cycle also demonstrated the activation at G1 and M phases. The activity change of cAMP-dependent protein kinase (PKA) was reciprocal to that of CaMK-III. These results indicated: (1) the activity of CaMK-III was cell cycle-dependent and (2) the level of eEF-2 phosphorylation followed the kinase activity change. Therefore, the elongation step of protein synthesis might be cell cycle dependently regulated.

• Transactions of the Korean Society of Automotive Engineers
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• v.2 no.6
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• pp.94-101
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• 1994

About vibration model of Six-degrees-of-freedom(DOF), in mass load, examined results for knowing dynamic interference and response variation is as follows; In case of putting mass load upon the object, experimented results on two-degrees-of-freedom of the translation-1 direction and the rotation-1 direction at open-loop-control system, about 0.19 arcsed in input of the translation-$0.1{\mu}m$ and $0.022{\mu}m$ on input of the rotation-0.5 arcsec, the justicse of motion equation is acknowledged as confirming the appearance of the interference-$0.022{\mu}m$. In establishing calculation of transformation matrix by using analogue circuit, as simulating results that used incomplete differentiation, interference is $1.7{\times}10^{-3}$ arcsec on input of the translation-$0.1{\mu}m$ and $1.4{\times}10^{4}{\mu}m$ on input of the rotation-0.5 arcsec in open-loop-control system. Also it is $4.2{\times}10^{-4}$ arcsec on input of the translation-$0.1{\mu}m$ and $5.6{\times}10^{-5}{\mu}m$ on input of the rotation-0.5 arcesc in closed-loop-control system. As closed-loop-control system is better than open-loop-control system, equivalent accordance is confirmed on original response. Finally, fundamental validity of this theory is acknowledged.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.3B
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• pp.275-286
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• 2011

As the 21st century paradigm of healthcare service changes from post-therapeutic treatment to disease prevention and management in advance, the M2M-based u-healthcare application service is increasingly important. M2M-based u-healthcare application service uses mobile handsets equipped with sensors to measure vital information, and the medical staff in remote locations can manage the health of the patient or the public in real time. In this paper, IEEE/HL7 translation gateway, utilizing the gateway based on M2M u-healthcare application service structure, which is based on international standards, has been designed and implemented. Specifically, the gateway between ISO/IEEE 11073 standards and ANSI HL7 has been developed. The former defines the protocol for the exchange of information between the agent device and the manger devices for measuring and handling biological signal, and the latter defines the application layer protocol for the exchange of various healthcare information systems. Finally, in order to demonstrate the functionality of the proposed architecture, the M2M-based U-healthcare application service based on IEEE/HL7 translation gateway, utilizing the gateway, has been developed which can measure, collect and process a variety of vital signs such as ECG or SpO2.

• BMB Reports
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• v.50 no.11
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• pp.539-545
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• 2017

The Integrated Stress Response (ISR) refers to a signaling pathway initiated by stress-activated $eIF2{\alpha}$ kinases. Once activated, the pathway causes attenuation of global mRNA translation while also paradoxically inducing stress response gene expression. A detailed analysis of this pathway has helped us better understand how stressed cells coordinate gene expression at translational and transcriptional levels. The translational attenuation associated with this pathway has been largely attributed to the phosphorylation of the translational initiation factor $eIF2{\alpha}$. However, independent studies are now pointing to a second translational regulation step involving a downstream ISR target, 4E-BP, in the inhibition of eIF4E and specifically cap-dependent translation. The activation of 4E-BP is consistent with previous reports implicating the roles of 4E-BP resistant, Internal Ribosome Entry Site (IRES) dependent translation in ISR active cells. In this review, we provide an overview of the translation inhibition mechanisms engaged by the ISR and how they impact the translation of stress response genes.

• Wind and Structures
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• v.26 no.3
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• pp.179-190
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• 2018

Translation of tornadoes is an important feature in replicating the near-ground tornado flow field which has been simulated in previous studies based on Ward-type tornado simulators using relative motion of the ground plane. In this laboratory investigation, effects of translation on the near-ground tornado flow field were studied using the ISU Tornado Simulator that can physically translate over a ground plane. Two translation speeds, 0.15 m/s and 0.50 m/s, that scale up to those corresponding to slowly-moving tornadoes in the field were selected for this study. Compared with the flow field of a stationary tornado, the simulated tornado with translation had an influence on the spatial distribution and magnitude of the horizontal velocities, early reversal of the radial inflow, and expansion of the core radius. Maximum horizontal velocities were observed to occur behind the center of the translating tornado and on the right side of its mean path. An increase in translation speed, resulted in reduction of maximum horizontal velocities at all heights. Comparison of the results with previous studies that used relative motion of the ground plane for simulating translating tornadoes, showed that translation has similar effects on the flow field at smaller radial distances (~2 core radius), but different effects at larger radial distances (~4 core radius). Further, it showed that the effect of translation on velocity profiles is noticeable at and above an elevation of ~0.6 core radius, unlike those in studies based on the relative motion of the ground plane.